Analysis of extracellular polymeric substance (EPS) release in anaerobic sludge holding tank and its effects on membrane fouling in a membrane bioreactor (MBR)

2014 ◽  
Vol 70 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Byeong-Cheol Kim ◽  
Duck-Hyun Nam ◽  
Ji-Hun Na ◽  
Ki-Hoon Kang

Amongst sludge reduction strategies, the anaerobic side-stream sludge holding tank (SHT) is of particular interest because it has shown significant sludge reduction efficiency. However, due to the anaerobic and starving environment of the SHT, the release of extracellular polymeric substance (EPS) may be stimulated, and it may hamper the application of the SHT to the membrane bioreactor. In order to investigate the effect of sludge storage on EPS release, sludge samples from a pilot-scale sequencing batch reactor coupled with SHT was incubated in a series of bench-scale SHT reactors for different periods of time (0–24 h). The increase in EPS was not significant until 12 h of incubation (9.3%), while 40.9% of the increase was observed in the sample incubated for 24 h. The rapid increase in EPS concentration after 12 h indicates a greater rate of cell lysis than that with EPS consumption as substrate. Since inducing the initial stage of the endogenous phase within microorganisms is a key factor for the successful operation of the SHT for sludge reduction, the retention time for the SHT should be shorter than the time for the sudden increase in EPS release.

2006 ◽  
Vol 1 (3) ◽  
Author(s):  
S.R. Chae ◽  
H.S. Chin

The main objective of this study was to investigate reasons of mitigated membrane fouling based on a mathematical prediction of daily excess sludge (DES) and extracellular polymeric substance (EPS) accumulation in a laboratory-scale vertical submerged membrane bioreactor (VSMBR) fed with glucose. Yield coefficient, growth rate, and decay rate of autotrophic and heterotrophic micro-organisms were estimated by measuring oxygen uptake rate (OUR). From kinetic estimation, it was concluded that the DES from the VSMBR was only 4% of the total DES produced by the activated sludge process (ASP). This resulted from the lower yield biomass and the higher decay of heterotrophic biomass in the VSMBR. In part of the experiment, transmembrane pressure (TMP) was affected by a rapid increase in EPS concentration. Later, a second rapid increase of TMP was observed although EPS concentration was stable indicating that the second increase of TMP was caused by interfiber clogging other than EPS accumulation.


2004 ◽  
Vol 4 (1) ◽  
pp. 135-142 ◽  
Author(s):  
H. Shin ◽  
S. Kang ◽  
C. Lee ◽  
J. Lim

The submerged membrane bioreactor is one of the recent technologies for domestic wastewater treatment. In this study, the performance of the pilot-scale submerged membrane bioreactor coupled with sequencing batch reactor (SM-SBR) was investigated. The reactor was operated in sequencing batch modes with a 3-hour cycles consisting of anoxic and aerobic conditions to treat organics, nitrogen and phosphorus. Despite large fluctuations in influent conditions, COD removal was found to be higher than 95%. Sufficient nitrification was obtained within a few weeks after start-up and during the stable period. Moreover, complete nitrification occurred despite of short aeration time. Total nitrogen (TN) removal efficiency was up to 85%. The insufficient organic loading caused by the membrane fouling led to the increase of HRT, leading to endogenous respiration and/or deactivation of nitrifying microorganisms. DGGE patterns confirmed the shift in microbial community structure. The ammonia-oxidizers (i.e. Nitrospira) became dominant in the mixed liquor during long-term operations. Nitrification and denitrification processes were greatly affected by the temperature, while organic removal and phosphorus removal efficiencies were relatively stable below 15°C.


2003 ◽  
Vol 47 (1) ◽  
pp. 139-144 ◽  
Author(s):  
H. Shin ◽  
S. Kang

The performance of the pilot-scale submerged membrane coupled with sequencing batch reactor (SM-SBR) for upgrading effluent quality was investigated in this study. The reactor was operated with 3-hour cycle with alternating anoxic and aerobic conditions to treat organics, nitrogen and phosphate. Despite various influent characteristics, COD removal was always higher than 95%. Sufficient nitrification was obtained within a few weeks after start-up and during the stable period, complete nitrification occurred despite short aeration time. Total nitrogen (TN) removal efficiency was reached up to 85%. Membrane flux was critical for TN removal so that the decrease of flux by membrane fouling led to increase of HRT, and it caused the endogenous respiration of microorganisms such as nitrifying bacteria. The stirred cell test revealed the significant role of the soluble fraction in membrane permeability and dissolved solids played a major role in the short-term fouling mechanism. The cake resistance by the soluble COD fraction of supernatant or soluble microbial products (SMP) was investigated as a major part of total resistance.


2014 ◽  
Vol 1065-1069 ◽  
pp. 3131-3135 ◽  
Author(s):  
Ying Zhang Wang ◽  
Xin Yue Zhu ◽  
Bai Ge Su ◽  
Zhi Qiang Liu ◽  
Hu Liu

Sludge Concentration (MLSS) is one of major parameters for operation of Anoxic/Aerobic-Membrane Bioreactors(A/O-MBR)and it influences the removal efficiency of pollutants and the rate of membrane fouling as well. This paper focuses on the effects of different MLSS on the treatment of domestic wastewater, the quantity and composition of extracellular polymeric substance (EPS), and the variation of the rate of membrane fouling. It also includes a preliminary analysis of the correlation between EPS and membrane fouling to provide reference for future studies of the mechanism of membrane fouling.


2021 ◽  
Vol 16 (1) ◽  
pp. 329-341
Author(s):  
Tukaram P. Chavan ◽  
Ganpat B. More ◽  
Sanjaykumar R. Thorat

The present investigation was carried out to assess the operation of a pilot-scale submerged membrane bioreactor (SMBR) for the treatment of reactive dye and textile wastewater. The operation of SMBR model was conducted by using a polyethersulfone (PES) hollow fibre membrane with continuous flow mode at different HRTs at 8, 6 and 4 h, for 90 days. During the entire operation, the average permeate flux, TMP, F/M ratio and OLR was found to be 19 (L/m²/h), 2.6 (psi), 0.10 (g BOD/(g MLSS•d) and 0.89 (kg BOD/m³.d), respectively. The variations in the permeate flux, TMP, F/M ratio and OLR have not adversely effects on the operation of the SMBR model. Throughout the entire operation, despite the TP, TDS and conductivity, the high amount of COD (82%), BOD (86%), NO3-N (79%), TSS (98%), turbidity (97%) and colour (79%), removal was achieved. The permeate flux was declined by membrane fouling and it was recovered by chemical cleaning as well as regular backwashing during the entire operation. The results obtained from the study concluded that the hollow fibre ultrafiltration polyethersulfone (PES) membrane shows good performance while treating textile wastewater along with reactive dye solution.


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