Effect of using different proportions of inoculum during bioleaching on sludge dewaterability

2018 ◽  
Vol 2017 (3) ◽  
pp. 802-811 ◽  
Author(s):  
Qingfang Zhang ◽  
Wenfeng Yang ◽  
Qiyong Yang ◽  
Tianfeng Wang ◽  
Shenliang Chen ◽  
...  
Keyword(s):  
Crucial Role ◽  
Extracellular Polymeric Substances ◽  
Reduction Potential ◽  
Specific Resistance ◽  
Sludge Dewatering ◽  
Capillary Suction ◽  
Geological Materials ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Sludge Dewaterability

Abstract Bioleaching, the addition of bacteria to geological materials, has been applied to sludge to remove metals and improve upon sludge dewaterability. This paper investigates the effect of using different quantities of inoculum (bacteria) during bioleaching on sludge dewaterability. The analysis was based on bioleaching experiments conducted in a 20 L bio-reactor using different quantities of inoculum (20%, 10%, 5%, 2%, 0%). Changes in pH, oxidation reduction potential (ORP), capillary suction time (CST), specific resistance to filtration (SRF) and extracellular polymeric substances (EPS) were determined to gauge sludge dewatering. Results indicate that sludge dewaterability during the 2%, 10%, and 20% inoculum experiments declined through time. Decreased dewaterability is attributed to increases in the quantity of proteins and polysaccharides in slime EPS. Dewaterability improved during the 5% inoculum experiment, and reached a maximum when pH was 2.3. During this latter experiment, CST and SRF were reduced by 74% and 62%, respectively, in comparison to control conditions, while total EPS content decreased by 71%. The decrease in total EPS was primarily due to a decrease in proteins associated with tightly bound EPS (TB-EPS). Thus, changes in the amount of proteins in TB-EPS and sludge pH played a crucial role in sludge dewaterability.

scholarly journals The influence of different sludge concentrations on its dewaterability during bioleaching

2020 ◽  
Vol 81 (12) ◽  
pp. 2585-2598
Author(s):  
Wenfeng Yang ◽  
Liyuan Zeng ◽  
Weihao Zhang ◽  
Qiyong Yang ◽  
Tianfeng Wang ◽  
...  
Keyword(s):  
Extracellular Polymeric Substances ◽  
Reduction Potential ◽  
High Efficiency ◽  
Specific Resistance ◽  
Principal Component ◽  
Buffering Capacity ◽  
Capillary Suction ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Sludge Dewaterability

Abstract Bioleaching, a technologically and economically feasible technology, is considered as the high efficiency method to improve dewaterability in sewage sludge. The objective of this study was to investigate the effect of different sludge concentrations on bioleaching dewaterability and understand the mechanism of the effect of bioleaching on sludge dewaterability. Variation in pH, oxidation-reduction potential (ORP), capillary suction time (CST), specific resistance to filtration (SRF) and different fractions of extracellular polymeric substances (EPS) including slime EPS (S-EPS), loosely bound EPS (LB-EPS), and tightly bound EPS (TB-EPS) were determined. Different sludge concentrations (5, 10, 15, 20 and 30 g·L−1) were selected to investigate during bioleaching. Results indicated that sludge buffering capacity significantly inhibited bioleaching efficiency as sludge concentrations increased. Optimum enhancements in sludge dewaterability were observed during the 10 g·L−1 sludge concentration treatment, and reached a maximum when the pH was 2.11. The variation of different fractions of EPS revealed that the ratio of S-EPS/TB-EPS significantly affected sludge dewaterability. Principal component analysis and Pearson's correlation analysis both provided evidence that the higher TB-EPS followed by a very large reduction was positively correlated with sludge dewaterability. However, the increase of protein and DNA in S-EPS content was negatively correlated with sludge dewaterability.

scholarly journals Synergetic Effect of Combined CaO2 and Microwave Treatment on Waste Active Sludge Dewaterability and Organic Contaminants’ Removal

2021 ◽  
Vol 9 ◽  
Author(s):  
Jie Wang ◽  
Kankan Shang ◽  
Liangjun Da ◽  
Xingguo Liu ◽  
Yongjing Zhao ◽  
...  
Keyword(s):  
Organic Contaminants ◽  
Superoxide Anion ◽  
Extracellular Polymeric Substances ◽  
Synergetic Effect ◽  
Microwave Treatment ◽  
Sludge Dewatering ◽  
Active Sludge ◽  
Capillary Suction ◽  
Contaminants Removal ◽  
Sludge Dewaterability

This study investigated the synergetic effect of the combined calcium peroxide (CaO2) and microwave (MW) treatment on waste active sludge dewatering properties and organic contaminants’ removal. The optimal sludge dewaterability was obtained at CaO2 (20 mg/gVSS)/MW (70°C), and the capillary suction time decreased by 52% compared with raw sludge. Further investigation indicated that total extracellular polymeric substances (EPS), tightly bound EPS, total protein, and protein present in tightly bound EPS were closely correlated with sludge dewaterability. Tryptophan, aromatic protein–like substances and humic acid–like substances were the key compounds that affect sludge dewaterability. The charge neutralization and bridge effect of cation ions were strengthened when combined with MW irradiation. In addition, it was revealed that MW facilitated CaO2 to produce more hydroxyl and superoxide anion radicals. This study confirmed CaO2/MW to be an effective way to improve sludge dewatering and remove organic pollutants from sludge.

Enhancement of sewage sludge dewaterability by fungal conditioning with Penicillium simplicissimum NJ12: From bench- to pilot-scale consecutive multi-batch investigations

2021 ◽  
Author(s):  
Neng Tao ◽  
Xiu Wu ◽  
Feng Zhang ◽  
Zilei Pi ◽  
Jiaqi Wen ◽  
...  
Keyword(s):  
Extracellular Polymeric Substances ◽  
Volume Fraction ◽  
Linear Regression Analysis ◽  
Pilot Scale ◽  
Recycling Rate ◽  
Penicillium Simplicissimum ◽  
Fungal Treatment ◽  
Sludge Dewatering ◽  
Capillary Suction ◽  
Sludge Dewaterability

Abstract Bench- and pilot-scale successive multi-batch trials were conducted to investigate the performance and sustainability of fungal conditioning with Penicillium simplicissimum NJ12 for improving sludge dewatering. The dominant factors affecting the sludge dewaterability improvement by P. simplicissimum NJ12 were also identified. Fungal treatment with P. simplicissimum NJ12 at a volume fraction of 5% of the inoculum greatly improved the sludge dewaterability. This improvement was characterized by sharp decreases in the specific resistance to filtration from 1.97 × 1013 to 3.52 × 1011 m/kg and capillary suction time from 32 to 12 s within 3 days. Stepwise multiple linear regression analysis showed that a marked decrease (58.8%) in the protein content in slime extracellular polymeric substances and an increase in the zeta potential of the sludge (from − 35 to − 10 mV) were the most important factors that improved the dewaterability of sludge after fungal treatment. Consecutive processes of fungal treatment could be realized by recirculating the fungal-treated sludge with a recycling rate of 1:2 (Vbiotreated sludge/Vtotal sludge). The treatment effectiveness was maintained only over three successive cycles, but replenishment with fresh P. simplicissimum NJ12 would be provided periodically at set batch intervals. These findings demonstrate the possibility of P. simplicissimum NJ12-assisted fungal treatment for enhancing sludge dewatering.

Correlation between oxidation-reduction potential values and sludge dewaterability during pre-oxidation

2019 ◽  
Vol 155 ◽  
pp. 96-105 ◽  
Author(s):  
Yang Lv ◽  
Keke Xiao ◽  
Jiakuan Yang ◽  
Yuwei Zhu ◽  
Kangyue Pei ◽  
...  
Keyword(s):  
Reduction Potential ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Sludge Dewaterability

scholarly journals Improvement of sludge dewaterability and disintegration efficiency using electrolytic zero-valent iron activated peroxymonosulfate

2021 ◽  
Author(s):  
Shaodong Guo ◽  
Yuxin Huang ◽  
Long Zhou ◽  
Xinghu Huang
Keyword(s):  
Extracellular Polymeric Substances ◽  
Specific Resistance ◽  
Three Dimensional ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Zero Valent Iron ◽  
Organic Substances ◽  
Fluorescence Excitation ◽  
Capillary Suction ◽  
Sludge Dewaterability

Abstract Electrolysis zero-valent iron activated peroxymonosulfate (EZVI-PMS) was applied to enhance sludge dewaterability and disintegration performance. Sludge dewaterability was characterized by capillary suction time (CST), specific resistance to filtration (SRF), and disintegration performance was explored by measuring sludge DNA content, ammonia nitrogen, chemical oxygen demand (COD), extracellular polymeric substances (EPS) and dissolved organic carbon (DOC). EPS including soluble EPS (SB-EPS), loosely bound EPS (LB-EPS), tightly bound EPS (TB-EPS) were analyzed by three dimensional fluorescence excitation-emission spectrum (3D-EEM) to confirm the proteins transformation tendency. DOC, protein and polysaccharide in EPSs were quantified to investigate the conditioning mechanism. The results showed that sludge CST and SRF were reduced significantly when the current was 0.2 A and PMS dosage was 130 mg/gDS with the reductions of 43.8% and 74.1%, respectively, and DNA was released from sludge cells to liquid phase. Mechanically, sludge TB-EPS converted to SB-EPS with DOC in TB-EPS decreasing from 367.0 mg/L to 210 mg/L, while DOC in SB-EPS increased from 44 mg/L to 167.4 mg/L. Besides, the changes of proteins and polysaccharides contents in SB-EPS and TB-EPS were similar to DOC, and protein in TB-EPS transformed to other protein-like or organic substances obviously.

scholarly journals Pyrolusite Bioleaching by an Indigenous Acidithiobacillus sp KL3 Isolated from an Indonesian Sulfurous River Sediment

2019 ◽  
Vol 19 (3) ◽  
pp. 712
Author(s):  
Endah Retnaningrum ◽  
Wahyu Wilopo
Keyword(s):  
Extracellular Polymeric Substances ◽  
River Sediment ◽  
Reduction Potential ◽  
Cell Attachment ◽  
Manganese Concentration ◽  
X Ray ◽  
Ph Values ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Scanning Electron

The manganese bioleaching process of pyrolusite from Kliripan, Indonesia using Acidithiobacillus sp KL3 was investigated. The influence pulp densities of pyrolusite (0.01, 0.02, 0.03 and 0.05 g/cm3) on the bioleaching processes were studied for 16 days. The reduction on pH values, the increasing of oxidation-reduction potential (ORP), sulfate and manganese concentration were analyzed. The manganese bioleaching mechanism of pyrolusite by the strain was monitored using Scanning Electron Microscope-Energy Dispersive-X-ray Spectroscopy (SEM-EDX). The results indicated that 0.02 g/cm3 of pyrolusite was considered to be the optimal pulp density for manganese bioleaching process. During this process, pH values decreased, furthermore resulted in increasing of ORP, the concentration of sulfate and manganese. SEM-EDX analysis clearly showed the evidence of directly bacterial cell attachment into the surface of pyrolusite. Extracellular polymeric substances (EPSs) were further founded on that surface. Sulfur elemental was oxidized by the strain which was then confirmed of resulting in solubilized manganese.

Comparison of lime and alum as oily sludge conditioners

1997 ◽  
Vol 36 (12) ◽  
pp. 117-124 ◽  
Author(s):  
Tay Joo Hwa ◽  
S. Jeyaseelan
Keyword(s):  
Specific Resistance ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Oily Sludge ◽  
Sludge Dewatering ◽  
Capillary Suction ◽  
Sludge Cake ◽  
Solids Content ◽  
Optimum Chemical

Chemical conditioning improves sludge dewatering. Choice of chemical conditioners is very much dependent on the characteristics of the sludges and the type of dewatering devices. Lime, alum, ferric chloride and polyelectrolytes are commonly used chemical conditioners. Anaerobic digested sludge samples collected from a sewage treatment plant with different oil contents varying from 1.8% to 8.0% by weight have been examined in the laboratory to find out their specific resistance, capillary suction time and filter yield. Lime and alum were used separately as conditioners. Different dosages of conditioner varying from 2% to 12% by weight were used to determine the optimum chemical dosage for varying oil contents. Lime dosages of about 6% were found to yield favourable characteristics. Addition of alum decreases the specific resistances and capillary suction times of oily sludges rapidly up to 4% dosages. Alum dosages beyond 4% only increase the solids content in the sludge cake and increase the sludge volume to be handled. A correlation between CST and specific resistance to filtration was established. CST can be measured easily and quickly in the laboratories. Using the CST and the correlation a quick prediction on dewaterability can be established.

scholarly journals In situ measurements of oxidation–reduction potential and hydrogen peroxide concentration as tools for revealing LPMO inactivation during enzymatic saccharification of cellulose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Adnan Kadić ◽  
Anikó Várnai ◽  
Vincent G. H. Eijsink ◽  
Svein Jarle Horn ◽  
Gunnar Lidén
Keyword(s):  
Reduction Potential ◽  
Enzymatic Saccharification ◽  
The State ◽  
Enzyme Inactivation ◽  
Ex Situ ◽  
Process Economics ◽  
Complete Inactivation ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential

Abstract Background Biochemical conversion of lignocellulosic biomass to simple sugars at commercial scale is hampered by the high cost of saccharifying enzymes. Lytic polysaccharide monooxygenases (LPMOs) may hold the key to overcome economic barriers. Recent studies have shown that controlled activation of LPMOs by a continuous H2O2 supply can boost saccharification yields, while overdosing H2O2 may lead to enzyme inactivation and reduce overall sugar yields. While following LPMO action by ex situ analysis of LPMO products confirms enzyme inactivation, currently no preventive measures are available to intervene before complete inactivation. Results Here, we carried out enzymatic saccharification of the model cellulose Avicel with an LPMO-containing enzyme preparation (Cellic CTec3) and H2O2 feed at 1 L bioreactor scale and followed the oxidation–reduction potential and H2O2 concentration in situ with corresponding electrode probes. The rate of oxidation of the reductant as well as the estimation of the amount of H2O2 consumed by LPMOs indicate that, in addition to oxidative depolymerization of cellulose, LPMOs consume H2O2 in a futile non-catalytic cycle, and that inactivation of LPMOs happens gradually and starts long before the accumulation of LPMO-generated oxidative products comes to a halt. Conclusion Our results indicate that, in this model system, the collapse of the LPMO-catalyzed reaction may be predicted by the rate of oxidation of the reductant, the accumulation of H2O2 in the reactor or, indirectly, by a clear increase in the oxidation–reduction potential. Being able to monitor the state of the LPMO activity in situ may help maximizing the benefit of LPMO action during saccharification. Overcoming enzyme inactivation could allow improving overall saccharification yields beyond the state of the art while lowering LPMO and, potentially, cellulase loads, both of which would have beneficial consequences on process economics.

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