Seeds as Economical Production Platform for Recombinant Proteins

2020 ◽  
Vol 27 (2) ◽  
pp. 89-104 ◽  
Author(s):  
Muhammad Sarwar Khan ◽  
Faiz Ahmad Joyia ◽  
Ghulam Mustafa

: The cost-effective production of high-quality and biologically active recombinant molecules especially proteins is extremely desirable. Seed-based recombinant protein production platforms are considered as superior choice owing to lack of human/animal pathogenic organisms, lack of cold chain requirements for transportation and long-term storage, easy scalability and development of edible biopharmaceuticals in plants with objective to be used in purified or partially processed form is desirable. This review article summarizes the exceptional features of seed-based biopharming and highlights the needs of exploiting it for commercial purposes. Plant seeds offer a perfect production platform for high-value molecules of industrial as well as therapeutic nature owing to lower water contents, high protein storage capacity, weak protease activity and long-term storage ability at ambient temperature. Exploiting extraordinarily high protein accumulation potential, vaccine antigens, antibodies and other therapeutic proteins can be stored without effecting their stability and functionality up to years in seeds. Moreover, ability of direct oral consumption and post-harvest stabilizing effect of seeds offer unique feature of oral delivery of pharmaceutical proteins and vaccine antigens for immunization and disease treatment through mucosal as well as oral route.

Vaccines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 252
Author(s):  
Ah-Young Kim ◽  
Hyejin Kim ◽  
Sun Young Park ◽  
Sang Hyun Park ◽  
Jae-Seok Kim ◽  
...  

A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. In contrast to O JC vaccine antigens stored in routinely used Tris-buffered or phosphate-buffered saline, those stored in Tris-KCl buffer showed extended shelf-life at both 4 °C and −70 °C. Additionally, the combined application of 10% sucrose and 5% lactalbumin hydrolysate could protect O JC 146S particles from massive structural breakdown in an aqueous state for up to one year. The stabilizer composition was also effective for other FMDV strains, including serotypes A and Asia 1. With this stabilizer composition, FMD vaccine antigens could be flexibly preserved during the general production process, pending status under refrigeration and banking under ultrafreezing.


Author(s):  
О.К. НИКУЛИНА ◽  
Л.И. ЧЕРНЯВСКАЯ

Необходимость длительного хранения большого количества сахарной свеклы на сахарных предприятиях приводит к высоким потерям свекломассы и сахара, а также к снижению технологических качеств сырья. Для снижения потерь сырья при хранении широкое распространение получили химические способы защиты, для оценки эффективности которых не выработано единого комплексного критерия. С целью разработки такого критерия исследована эффективность влияния обработки биологически активными препаратами Гембар, Полидез Плюс и КСД-2 на сохранность сахарсодержащего сырья. Для исследований использовали корнеплоды сахарной свеклы, выращенной в Республике Беларусь, хорошего технологического качества, которые обрабатывали одним из препаратов – КСД-2 концентрацией 5%, Гембар и Полидез Плюс концентрацией 0,15% при расходе 2 дм3 рабочего раствора на 1 т свеклы – и закладывали на краткосрочное, среднесрочное или длительное хранение в производственном кагате. Исследования фитопатологических показателей обработанных препаратами образцов корнеплодов и контрольных – необработанных корнеплодов и расчет потери массы и сахарозы после различной продолжительности хранения проводили в течение двух производственных сезонов. Установлено, что обработка позволяет снизить содержание гнилой массы и массу ростков соответственно на 73–100% и 81% при обработке препаратом Гембар, на 40–42% и 54% при обработке Полидезом Плюс, на 87–100% и 90–92% при обработке КСД-2; уменьшить потери массы свеклы и среднесуточные потери сахарозы соответственно на 6–46% и 22,2–53,3% при обработке препаратом Гембар, на 6–77% и 5,6–29,4% при обработке Полидез Плюс, на 19–76% и 33,3–69,2% при обработке КСД-2 в зависимости от продолжительности хранения и производственного сезона. Также установлено, что в контрольном образце содержание сахарозы в зависимости от продолжительности хранения снижалось, % к массе свеклы, на 0,19 при краткосрочном хранении, на 0,48 при среднесрочном и на 0,83 при длительном хранении, но после обработки препаратом КСД-2 образцы корнеплодов потеряли соответственно 0,05; 0,19 и 0,47% сахарозы к массе свеклы, что в 3,8; 2,5 и 1,8 раза меньше по сравнению с контролем. На основе результатов исследований для оценки эффективности влияния применяемого препарата на снижение деградации сахарной свеклы при различной продолжительности хранения (сохранность технологических качеств) предложено использование двух комплексных показателей – индекса технологического качества сахарной свеклы и индекса сохранности сахарной свеклы. По разработанным формулам комплексных показателей рассчитаны индексы сохранности сахарной свеклы для исследованных вариантов хранения. The need for long-term storage of a large amount of sugar beet at sugar enterprises leads to high losses of beet pulp and sugar, as well as to a decrease in the technological qualities of raw materials. To reduce the loss of raw materials during storage, chemical methods of protection have become widespread, for evaluating the effectiveness of which no single complex criterion has been developed. In order to develop such a criterion, the effectiveness of the effect of treatment with biologically active preparations Gembar, Polydez Plus and KSD-2 on the safety of sugar-containing raw materials was studied. For the research, we used sugar beet roots grown in the Republic of Belarus, of good technological quality, which were treated with one of the preparations – KSD-2 with a concentration of 5%, Gembar and Polydez Plus with a concentration of 0,15% at a consumption of 2 dm3of the working solution per 1 ton of beet – and laid for short-term, medium-term or long-term storage in a production cahat. Studies of phytopathological parameters of samples of root crops treated with preparations and control samples of untreated root crops and calculation of weight loss and sucrose after different storage periods were carried out during two production seasons. It was found that the treatment allows to reduce the content of rotten mass and the mass of sprouts respectively by 73–100% and 81% when treated with Gembar, by 40–42% and 54% when treated with Polydez Plus, by 87–100% and 90–92% when treated with KSD-2; to reduce beet mass losses and average daily sucrose losses, respectively, by 6–46% and 22,2–53,3% when treated with Gembar, by 6–77% and 5,6–29,4% when treated with Polydez Plus, by 19–76% and 33,3–69,2% when processing KSD-2 depending on the duration of storage and the production season. It was also found that in the control sample, the sucrose content, depending on the duration of storage, decreased, % by weight of beet: by 0,19 for short-term storage, by 0,48 for medium-term, and by 0,83 for long-term storage, but after treatment with KSD-2 the root crop samples lost 0,05; 0,19 and 0,47% by weight of beet respectively, which is 3,8; 2,5 and 1,8 times less compared to the control. Based on the research results, the use of two complex indicators – the index of technological quality of sugar beet and the index of safety of sugar beet-is proposed to assess the effectiveness of the effect of the drug used on reducing the degradation of sugar beet with different storage periods (preservation of technological qualities). According to the developed formulas of complex indicators, the indices of the safety of sugar beet for the studied storage options are calculated.


2009 ◽  
Vol 5 (7) ◽  
pp. 467-474 ◽  
Author(s):  
David Le Tallec ◽  
Diane Doucet ◽  
Abdelatif Elouahabi ◽  
Pol Harvengt ◽  
Michel Deschuyteneer ◽  
...  

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.


Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  

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