Accumulation Effects of Salicylic acid Saccharide Carboxylate on the Alkaloids in Catharanthus roseus Cells

An Experiment carried out in Al-Yarmook Area in Baghdad for the growing season 2014 to study the effects of foliar application of Salicylic acid and Humic acid on Vincristine and NPK content in Catharanthus roseus. A factorial experiment was applied including two factors according to The Complete Randomized Block Design, the first factor included four levels of salicylic acid 0, 25, 50, and 100 mg.L-1 which were represented with S0, S1, S2, and S3 sequentially, the second factor included three levels of Humic acid 0, 0.75, and 1.5 ml.L-1 and were represented with O0, O1, and O2 sequentially to make up 12 factors in total that were distributed randomly upon three replicates and each experimental unit included 8 plants. The results were analyzed using Genestat and the differences were tested according to (LSD) with a level of probability 5% and the results were as follows: Vincristine levels increased in the leaves of Catharanthus roseus as a result of foliar application of Salicylic acid and Humic as well NPK levels in leaves. Vinblastine was not detected in all treatments. The interaction effect between 100 mg.L-1 Salicylic acid and 1.5 ml.L-1Humic acid had an exceeding effect for all the studied parameters. It may be concluded that Salicylic and Humic acid may be used to increase Vincristine alkaloid content in Periwinkle plants at the levels 100 ml.L-1 and 1.5 mg.L-1 subsequently.

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Influence of L-Tryptophan and salicylic acid on secondary metabolites production from leaves induced callus of Catharanthus roseus L.G.Don in vitro

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2014.8.2.326 ◽

2014 ◽

Vol 8 (2) ◽

pp. 35-43

Author(s):

Emad H. Jassim ◽

Sami K. M. Ameen

Keyword(s):

Salicylic Acid ◽

Secondary Metabolites ◽

Catharanthus Roseus ◽

Leaf Explants ◽

Ms Medium ◽

Plant Leaves ◽

Fresh Weight ◽

Metabolites Production ◽

Different Levels

An experiment was conducted to steady the effect of L-Tryptophan and salicylic acid on callus induced on leaf explants of Catharanthus roseus. Callus induction was achieved by culturing true leaves of the plant on Murashige and Skoog (MS) medium supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin. The best medium to maintain callus was MS medium supplemented with 0.5 mg /L 2,4-D and 1.5 mg / L Kin. Different levels added to MS medium for each L-Tryptophan 0,200,300or400 mg /L and salicylic acid 0, 0.5,1or1.5mg /L. The medium supplemented with 30 g/ L sucrose was used as a control. Results showed the medium supplemented with 200 mgL of L-Tryptophan gave the highest quantity of Vincristine reached 48.66 µg/100 fresh weight of callus. MS medium content at the concentration 1 mg L of salicylic acid gave the highest level of Vinblastine recording 50.98 µg/100 fresh weight of callus. While MS medium supplemented with 0.5 mg /L of salicylic acid gave the highest level of Serpentine 24.76 µg/100 fresh weight of callus. The concentrations of active compound derived from plant leaves were much less than the concentrations produced by the callus grown in vitro. The concentration of Serpentine was 0.059 while Vinblastine was 0.183 and the concentration of Vincristine was 0.064.

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