Pyrogallol Induces Antimicrobial Effect and Cell Membrane Disruption on Methicillin Resistant Staphylococcus aureus (MRSA)

2021 ◽  
Vol 17 ◽  
Author(s):  
Yik-Ling Chew ◽  
Chairunnisa Arasi ◽  
Joo-Kheng Goh
Keyword(s):  
Antibacterial Activity ◽  
Ir Spectroscopy ◽  
Cell Membrane ◽  
Crystal Violet ◽  
Resistant Bacteria ◽  
Antibacterial Mechanism ◽  
Kinetic Assay ◽  
Crystal Violet Assay ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Background: Pyrogallol is present naturally in numerous plants and is also an important functional group in many polyphenol compounds. Objectives: The antibacterial activity, efficacy and mechanism of pyrogallol towards MRSA strains were evaluated in this study. Methods: Microbroth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Time-kill kinetic assay was adopted to determine the killing pattern of pyrogallol towards MRSA. The antibacterial mechanism was determined using scanning electron microscopy (SEM), Fourier Transform-Infrared (FT-IR) spectroscopy and crystal violet assay. Results: Pyrogallol exhibited strong antibacterial activity against MRSA with MIC and MBC 15.6 µg/mL. Pyrogallol could inhibit the exponential growth of MRSA and kill the bacterial cells at higher concentrations. Pyrogallol was found targeting the cell membrane fatty acids, proteins/peptides, polysaccharides/carbohydrates and peptidoglycan of cell walls in the antibacterial mechanism. This has been confirmed through SEM, FT-IR spectroscopy and crystal violet assay. Conclusion : Overall, the findings suggest that pyrogallol has the potential to be used as antibiotics which are used to treat multidrug-resistant bacteria.

scholarly journals Enhancing solubility and antibacterial activity using multi-component crystals of trimethoprim and malic acid

2021 ◽  
pp. 296-304
Author(s):  
Yori Yuliandra ◽  
Limson Jaya Hutabarat ◽  
Resty Ardila ◽  
Maria Dona Octavia ◽  
Erizal Zaini
Keyword(s):  
Antibacterial Activity ◽  
Solid State ◽  
Binary System ◽  
Ir Spectroscopy ◽  
Malic Acid ◽  
Diffusion Method ◽  
Crystal Formation ◽  
Pxrd Pattern ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Aim: To improve the solubility and antibacterial activity of trimethoprim (TMP) by preparing its multicomponent crystals with malic acid (MA). Methods: Multicomponent crystals of TMP-MA were prepared by solvent co-evaporation. The solid-state properties were characterised by powder X-ray diffraction (PXRD), differential thermal analysis (DTA), Fourier transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) analyses. The solubility was investigated in an aqueous medium, while the antibacterial activity against Escherichia coli was investigated using the agar disk diffusion method. Results: The PXRD pattern of the TMP-MA binary system differed from the starting materials, supporting the formation of a new crystalline phase (equimolar ratio). The DTA thermogram showed a single, sharp, endothermic peak at 212.5 °C attributable to the TMP-MA multicomponent crystal's melting point. FT-IR spectroscopy showed a solid-state interaction involving proton transfer between TMP and MA. The multicomponent crystal displayed a 2.5-fold higher solubility and had increased antibacterial activity compared to TMP alone. Conclusions: The TMP-MA binary system forms salt-type multicomponent crystals that significantly increase solubility and antibacterial activity. Multicomponent crystal formation is a viable technique for modifying the physicochemical properties of active pharmaceutical ingredients.

Multiscale exploration of hydrocarbons adsorption and hopping through the ZSM-5 channels - from Monte Carlo modelling to experiment

2021 ◽  
Author(s):  
Maciej Strzempek ◽  
Karolina A. Tarach ◽  
Kinga Góra-Marek ◽  
Fernando Rey ◽  
Miguel Palomino ◽  
...  
Keyword(s):  
Monte Carlo ◽  
Ir Spectroscopy ◽  
Aliphatic Hydrocarbons ◽  
Adsorption Studies ◽  
Ft Ir ◽  
Gravimetric Adsorption ◽  
Hydrocarbons Adsorption ◽  
Ft Ir Spectroscopy

Abstract In this article the results of the statistical MC modelling corroborated by the FT-IR spectroscopy and gravimetric adsorption studies of the low aliphatic hydrocarbons in ZSM-5 (Si/Al =28 or...

scholarly journals Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application

2021 ◽  
Vol 22 (4) ◽  
pp. 2191
Author(s):  
Jing Huang ◽  
Nairveen Ali ◽  
Elsie Quansah ◽  
Shuxia Guo ◽  
Michel Noutsias ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Outer Ring ◽  
Imaging Method ◽  
Fluorescence Lifetime Imaging ◽  
Drying Process ◽  
Vibrational Signal ◽  
Coating Deposition ◽  
Ft Ir ◽  
Sample Droplet ◽  
Ft Ir Spectroscopy

In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy.

FT-IR Spectroscopy As A Tool For The Study Of Metal Ions/D- Galactose Complexes

1989 ◽  
Author(s):  
Ning Xi ◽  
Shifu Weng ◽  
Jinguang Wu ◽  
Guangxian Xu
Keyword(s):  
Ir Spectroscopy ◽  
Metal Ions ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Calculation of the development of the Portlandite content based on FT-IR spectroscopy data

2020 ◽  
Author(s):  
Milena Pavlíková ◽  
Lenka Scheinherrová ◽  
Martina Záleská ◽  
Zbyšek Pavlík
Keyword(s):  
Ir Spectroscopy ◽  
Spectroscopy Data ◽  
Ft Ir ◽  
Ft Ir Spectroscopy
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