HPLC-DAD-Q-TOF/MS-Based Screening and Analysis of the Multiple Absorbed Bioactive Components in Rat Serum after Oral Administration of Xiexin Tang

2020 ◽  
Vol 16 (6) ◽  
pp. 712-723
Author(s):  
Xiaoyu Wang ◽  
Shujiao Li ◽  
Yuqing Wang ◽  
Rui Hu
Keyword(s):  
Intragastric Administration ◽  
Herbal Extracts ◽  
Bioactive Components ◽  
Rat Serum ◽  
Scutellariae Radix ◽  
Mechanism Research ◽  
Coptidis Rhizoma ◽  
Set Up ◽  
Reliable Analytical Method ◽  
Tof Ms

Background: Xiexin Tang (XXT) is a classic Traditional Chinese Medicine (TCM) formula that has been used in herbal clinics for more than 1800 years. Recently, many studies have investigated the pharmacological effects and chemical composition of XXT. However, there is little information about systematic studies on the material basis of its efficacy. In the present study, the serum pharmacochemistry technique and HPLC-DAD-Q-TOF/MS were performed to screen and analyze the multiple absorbed bioactive components and metabolites of orally dosed XXT in rat serum. Methods: Bio-samples and herbal extracts were analyzed and detected by HPLC-DAD-Q-TOF/MS. Upon comparison of the chromatograms of the single-constituent decoctions with that of the XXT formulation, the peak quantity and peak intensity of the formulated decoction showed some variation from those of the single-constituent decoctions. Results: Twenty-one serum-adsorbed constituents were identified after intragastric administration of herbal extracts, of which 8 originated from Rhei Radix et Rhizoma (RRR), 5 from Coptidis Rhizoma (CR), and 8 from Scutellariae Radix (SR). The results showed that the main adsorbed constituents in the serum were anthraquinones, anthrones, chromones, and butyrophenones, alkaloids, and flavonoids. Conclusion: The results demonstrate that an effective and reliable analytical method is set up for screening the bioactive components of Chinese herbal medicine, which provided a meaningful basis for further pharmacology and active mechanism research of XXT.

Inhibitory effects of Coptidis Rhizoma on the intestinal absorption and metabolism of Scutellariae Radix

2021 ◽  
pp. 113785
Author(s):  
Wei Zheng ◽  
Guixia Sun ◽  
Jianhua Chen ◽  
Zhihui Li ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Inhibitory Effects ◽  
Scutellariae Radix ◽  
Coptidis Rhizoma

scholarly journals Rapid identification of direct-acting pancreatic protectants from Cyclocarya paliurus leaves tea by the method of serum pharmacochemistry combined with target cell extraction

2020 ◽  
Vol 18 (1) ◽  
pp. 265-274
Author(s):  
Wei-hong Chen ◽  
Zhen Luo ◽  
Zi-Wan Ning ◽  
Jiao Peng ◽  
Xiao-peng Hu ◽  
...  
Keyword(s):  
Target Cell ◽  
Rapid Identification ◽  
Target Cells ◽  
Bioactive Components ◽  
Β Cell ◽  
Cyclocarya Paliurus ◽  
Cell Extraction ◽  
Direct Acting ◽  
Apoptotic Effects ◽  
Tof Ms

AbstractExtracts of Cyclocarya paliurus (CP) leaves, a popular sweet tea, inhibit pancreatic β cell apoptosis and have potent hypoglycemic effects, but the identities of the anti-apoptotic bioactive components are still unknown. In the present study, a method using UPLC-Q-TOF/MS based on serum pharmacochemistry combined with target cell extraction was established to rapidly identify direct-acting pancreatic protectants from CP. After orally administering a set amount of CP extract to rats, blood samples were collected to characterize the components that can be absorbed into the blood using UPLC-Q-TOF/MS. Also, target cells (pancreatic β NIT-1 cells) were incubated with CP extract for 24 hours, and cells were collected to identify the components that can bind to the cells using UPLC-Q-TOF/MS. Finally, to evaluate the protective effect of the bioactive components of CP, MTT and TUNEL assays were performed on treated NIT-1 cell induced by streptozotocin (STZ). Three potential direct-acting pancreatic protectants -- kaempferol, quercetin, quadranoside IV -- were identified, and anti-apoptotic effects of kaempferol and quercetin were confirmed in STZ-induced NIT-1 cells. The findings indicate that this combined approach is a feasible, rapid, and expedient tool for capturing potential direct-acting components from natural products such as those from CP leaves.

scholarly journals Rapid Classification of Clostridioides difficile Strains Using MALDI-TOF MS Peak-Based Assay in Comparison with PCR-Ribotyping

2021 ◽  
Vol 9 (3) ◽  
pp. 661
Author(s):  
Adriana Calderaro ◽  
Mirko Buttrini ◽  
Monica Martinelli ◽  
Benedetta Farina ◽  
Tiziano Moro ◽  
...  
Keyword(s):  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Clostridioides Difficile ◽  
Pcr Ribotyping ◽  
Typing Methods ◽  
Set Up ◽  
Tof Ms

Typing methods are needed for epidemiological tracking of new emerging and hypervirulent strains because of the growing incidence, severity and mortality of Clostridioides difficile infections (CDI). The aim of this study was the evaluation of a typing Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS (T-MALDI)) method for the rapid classification of the circulating C. difficile strains in comparison with polymerase chain reaction (PCR)-ribotyping results. Among 95 C. difficile strains, 10 ribotypes (PR1–PR10) were identified by PCR-ribotyping. In particular, 93.7% of the isolates (89/95) were grouped in five ribotypes (PR1–PR5). For T-MALDI, two classifying algorithm models (CAM) were tested: the first CAM involved all 10 ribotypes whereas the second one only the PR1–PR5 ribotypes. Better performance was obtained using the second CAM: recognition capability of 100%, cross-validation of 96.6% and agreement of 98.4% (60 correctly typed strains, limited to PR1–PR5 classification, out of 61 examined strains) with PCR-ribotyping results. T-MALDI seems to represent an alternative to PCR-ribotyping in terms of reproducibility, set up time and costs, as well as a useful tool in epidemiological investigation for the detection of C. difficile clusters (either among CAM included ribotypes or out-of-CAM ribotypes) involved in outbreaks.

Metabolomics-based mechanisms exploration of Huang-Lian Jie-Du decoction on cerebral ischemia via UPLC-Q-TOF/MS analysis on rat serum

2018 ◽  
Vol 216 ◽  
pp. 147-156 ◽  
Author(s):  
Baojie Zhu ◽  
Huiting Cao ◽  
Limin Sun ◽  
Bo Li ◽  
Liwei Guo ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Rat Serum ◽  
Ms Analysis ◽  
Tof Ms

scholarly journals Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Fang Geng ◽  
Fashan Wang ◽  
Taobo Zou ◽  
Kuiyuan Zhu ◽  
Wei Ma ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ammonium Acetate ◽  
Gradient Elution ◽  
Rapid Identification ◽  
Ionization Mass ◽  
Pharmacological Effects ◽  
Bioactive Components ◽  
Rat Serum ◽  
Esi Ms ◽  
Acquity Uplc

To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) under a gradient elution. The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V). Twelve absorbed compounds and four metabolites were found. Seven of the absorbed compounds were identified by ESI-MS. The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.

scholarly journals Challenges for Biomarker Discovery in Body Fluids Using SELDI-TOF-MS

2010 ◽  
Vol 2010 ◽  
pp. 1-15 ◽  
Author(s):  
Muriel De Bock ◽  
Dominique de Seny ◽  
Marie-Alice Meuwis ◽  
Jean-Paul Chapelle ◽  
Edouard Louis ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Biological Fluids ◽  
Protein Profiling ◽  
Sample Collection ◽  
Sample Handling ◽  
Biomarker Identification ◽  
The Past ◽  
Set Up ◽  
And Storage ◽  
Tof Ms

Protein profiling using SELDI-TOF-MS has gained over the past few years an increasing interest in the field of biomarker discovery. The technology presents great potential if some parameters, such as sample handling, SELDI settings, and data analysis, are strictly controlled. Practical considerations to set up a robust and sensitive strategy for biomarker discovery are presented. This paper also reviews biological fluids generally available including a description of their peculiar properties and the preanalytical challenges inherent to sample collection and storage. Finally, some new insights for biomarker identification and validation challenges are provided.

Analysis of six bioactive components in Semen Ziziphi Spinosae by UPLC-ELSD and UPLC-Q/TOF-MS

2014 ◽  
Vol 6 (15) ◽  
pp. 5856-5864 ◽  
Author(s):  
Xiangsheng Zhao ◽  
Liwei Xie ◽  
Haifeng Wu ◽  
Weijun Kong ◽  
Meihua Yang
Keyword(s):  
Analytical Method ◽  
Ultrasonic Extraction ◽  
Bioactive Components ◽  
Tof Ms

A fast and validated multicomponent analytical method was developed based on ultrasonic extraction, detection by UPLC-ELSD and confirmation by UPLC-Q/TOF-MS.

Sign in / Sign up

Export Citation Format

Share Document