Multiple neonicotinoids in children’s cerebro-spinal fluid, plasma, and urine

Background Neonicotinoids (NN) are selective neurotoxic pesticides that bind to insect but also mammal nicotinic acetycholine receptors (nAChRs). As the most widely used class of insecticides worldwide, they are ubiquitously found in the environment, wildlife, and foods, and thus of special concern for their impacts on the environment and human health. nAChRs are vital to proper brain organization during the prenatal period and play important roles in various motor, emotional, and cognitive functions. Little is known on children’s contamination by NN. In a pilot study we tested the hypothesis that children’s cerebro-spinal fluid (CSF) can be contaminated by NN. Methods NN were analysed in leftover CSF, blood, and urine samples from children treated for leukaemias and lymphomas and undergoing therapeutic lumbar punctions. We monitored all neonicotinoids approved on the global market and some of their most common metabolites by ultra-high performance liquid chromatography-tandem mass spectrometry. Results From August to December 2020, 14 children were consecutively included in the study. Median age was 8 years (range 3–18). All CSF and plasma samples were positive for at least one NN. Nine (64%) CSF samples and 13 (93%) plasma samples contained more than one NN. Thirteen (93%) CSF samples had N-desmethyl-acetamiprid (median concentration 0.0123, range 0.0024–0.1068 ng/mL), the major metabolite of acetamiprid. All but one urine samples were positive for ≥ one NN. A statistically significant linear relationship was found between plasma/urine and CSF N-desmethyl-acetamiprid concentrations. Conclusions We have developed a reliable analytical method that revealed multiple NN and/or their metabolites in children’s CSF, plasma, and urine. Our data suggest that contamination by multiple NN is not only an environmental hazard for non-target insects such as bees but also potentially for children.

ANALYTICAL DETERMINATION OF THE MICRORELIEF PARAMETERS FORMED AS A RESULT OF CENTERLESS GRINDING OF THE FULL SPHERE

При технологической подготовке операций чистовой и отделочной обработки деталей абразивными инструментами очень важными задачами являются прогнозирование ее результатов в зависимости от назначенных режимов и назначение режимов в зависимости от требуемого качества обработанных поверхностей. Имеющиеся многочисленные справочные материалы такого характера получены в лабораторных условиях, носят дискретный характер, не учитывают специфическую топографию поверхностей абразивных инструментов и множества других условий и поэтому недостаточно достоверны. На их основе возможно планировать технологический процесс только предварительно, а затем неизбежно требуется уточнять его параметры экспериментальным путем, что приводит к значительному удорожанию процесса подготовки производства. Наличие достоверной аналитической методики позволяет кратно снизить эти затраты, и поэтому она настоятельно необходима не только и даже не столько при выполнении научных исследований, сколько в реальном производстве. В статье аналитически определены параметры микрорельефа, образующегося в результате бесцентрового шлифования полной сферы. Уточнено влияние процесса выхаживания на формирование шероховатости сферы. Приведена методика расчета среднеарифметического отклонения микропрофиля сферической поверхности In the technological preparation of the operations of finishing processing of parts with abrasive tools, it is very important to predict its results depending on the assigned modes and the purpose of modes depending on the required quality of the processed surfaces. The available numerous reference materials of this nature were obtained in laboratory conditions, are discrete in nature, do not take into account the specific topography of the surfaces of abrasive tools and many other conditions, and therefore are not reliable enough. On their basis, it is possible to plan the technological process only in advance, and then inevitably it is necessary to refine its parameters experimentally, which leads to a significant increase in the cost of the production preparation process. The availability of a reliable analytical method allows you to multiply these costs, and therefore it is urgently needed not only and even not so much when performing scientific research, but in real production. In the article, we analytically determine the parameters of the microrelief formed as a result of centerless grinding of a full sphere. We determined the influence of the nursing process on the formation of the sphere roughness. We present a method for calculating the arithmetic mean deviation of the micro-profile of a spherical surface

Doping control analysis for small peptides in human urine using LC-HRMS with parallel reaction monitoring mode: screening and confirmation

This study described a reliable analytical method, which combines solid-phase extraction (SPE) with liquid chromatography-high resolution mass spectrometry (LC-HRMS) employing the parallel reaction monitoring (PRM) mode, for screening 41 small...

A multi-residue analytical method for extraction and analysis of pharmaceuticals and other selected emerging contaminants in sewage sludge

The present study developed and applied a reliable analytical method for the analysis of 44 ECs in sewage sludge. The most abundant compounds were antibiotics, anti-inflammatories and antihypertensives that showed seasonal variability.

Application of LC–MS/MS method for determination of dihydroartemisin in human plasma in a pharmacokinetic study

Aim: Dihydroartemisinin (DHA) was also found therapeutic potential for the treatment of systemic lupus erythematosus (SLE). To assess the pharmacokinetic profile of DHA, the concentration of DHA in plasma of SLE patients needed be accurately determined based on a rapid and reliable analytical method. Experimental method & results: Developed method utilizes stable isotope-labeled internal standards and SPE method for sample preparation, applied XBridge C18 column (2.1 × 50 mm, 3.5 μm) for chromatography separation. Detection of the analytes was achieved by an AB Sciex 4000 mass spectrometer under positive electrospray ionization mode. The method was validated in accordance with international guidelines on bioanalytical methods validations. Conclusion: DHA concentrations in human plasma of Chinese SLE patients were quantified by developed LC–MS/MS (no. 2016L02562).

Determination of morphine and its metabolites in the biological samples: an updated review

Morphine (MO) as an opioid analgesic is used for the treatment of moderate-to-severe pains, particularly cancer-related pains. Pharmacologic studies on MO are complicated due to drugs binding to the protein or metabolization to active metabolites, and even inter-individual variability. This necessitates the selection of a reliable analytical method for monitoring MO and the concentrations of its metabolites in the biological samples for the pharmacokinetic or pharmacodynamic investigations. Therefore, this study was conducted to review all the analytical research carried out on MO and its metabolites in the biological samples during 2007–2019 as an update to the study by Bosch et al. (2007).

HPLC-DAD-Q-TOF/MS-Based Screening and Analysis of the Multiple Absorbed Bioactive Components in Rat Serum after Oral Administration of Xiexin Tang

Background: Xiexin Tang (XXT) is a classic Traditional Chinese Medicine (TCM) formula that has been used in herbal clinics for more than 1800 years. Recently, many studies have investigated the pharmacological effects and chemical composition of XXT. However, there is little information about systematic studies on the material basis of its efficacy. In the present study, the serum pharmacochemistry technique and HPLC-DAD-Q-TOF/MS were performed to screen and analyze the multiple absorbed bioactive components and metabolites of orally dosed XXT in rat serum. Methods: Bio-samples and herbal extracts were analyzed and detected by HPLC-DAD-Q-TOF/MS. Upon comparison of the chromatograms of the single-constituent decoctions with that of the XXT formulation, the peak quantity and peak intensity of the formulated decoction showed some variation from those of the single-constituent decoctions. Results: Twenty-one serum-adsorbed constituents were identified after intragastric administration of herbal extracts, of which 8 originated from Rhei Radix et Rhizoma (RRR), 5 from Coptidis Rhizoma (CR), and 8 from Scutellariae Radix (SR). The results showed that the main adsorbed constituents in the serum were anthraquinones, anthrones, chromones, and butyrophenones, alkaloids, and flavonoids. Conclusion: The results demonstrate that an effective and reliable analytical method is set up for screening the bioactive components of Chinese herbal medicine, which provided a meaningful basis for further pharmacology and active mechanism research of XXT.

UHPLC–MS/MS method to determine FP-208 in human plasma and its application to a pharmacokinetic study

Aim: FP-208 is a novel and effective small-molecule inhibitor blocking the mammalian target of rapamycin complex-1/mammalian target of rapamycin complex-2/PI3Ka. To investigate the pharmacokinetic profile of FP-208, a rapid and reliable analytical method was needed to be established to determine FP-208 in the plasma of patients with solid tumors. Materials & methods: FP208 was separated on a charged surface hybrid (CSH) C18 column (2.1 mm × 50 mm, 1.7 μm) after the plasma samples were purified using a protein precipitation method. Detection was performed on an AB Sciex 5500 mass spectrometer in the positive electrospray ionization mode. The established method was validated according to the bioanalytical guidelines. Conclusion: For the first time, the developed and validated method was successfully applied in the first-in-human study for FP-208 in patients with solid tumors after oral administration (Number: CTR20180683).

Use of IHF-QD Microscopic Analysis for the Detection of Food Allergenic Components: Peanuts and Wheat Protein

The aim of the study was to analytically evaluate quantum dots in immunohistofluorescence (IHF-QD) microscopic imaging as detectors of food allergens—peanut and wheat. The experiment was designed as two in silico experiments or simulations: (a) models of pastry samples were prepared with the addition of allergenic components (peanut and wheat protein components) and without the addition of allergenic components, and (b) positive and negative commercial samples underwent food allergen detection. The samples from both simulations were tested by the ELISA and IHF-QD microscopic methods. The primary antibodies (secondary antibodies to a rabbit Fc fragment with labeled CdSe/ZnS QD) were labelled at 525, 585, and 655 nm emissions. The use of quantum dots (QDs) has expanded to many science areas and they are also finding use in food allergen detection, as shown in the study. The study indicated that differences between the ELISA and IHF-QD microscopic methods were not observable among experimentally produced pastry samples with and without allergenic components, although differences were observed among commercial samples. The important value of the study is certainly the differences found in the application of different QD conjugates (525, 585, and 655). The highest contrast was found in the application of 585 QD conjugates that can serve for the possible quantification of present food allergens—peanuts and wheat. The study clearly emphasized that QD can be used for the qualitative detection of food allergens and can represent a reliable analytical method for food allergen detection in different food matrixes.