Tetrac-Conjugated Quantum Dots for Diagnosis and Treatment of Pancreatic Cancer: An In Vitro Evaluation

A nanotheranostic system is a new system that combines both diagnosis and treatment of a malignant disease, e.g. cancer, by exploitation of the unusual properties of nano-sized particles. In this project, we have developed a novel nanotheranostic system based on quantum dots (QDs) coated with drug-loaded polymer. We first synthesized a double-group functionalized amphiphilic triblock copolymer and loaded it with the anti-tumour drug paclitaxel (PTX) and lipoic acid (LA) to obtain the prodrug mPEG-block-PCL-(graft-PTX)-block-PLA-(graft-LA) (mPEG: methoxy polyethylene glycol, PLA: poly(norbornene-lactide)s). When exposed to UV light (365 nm), the disulfide bond of LA was broken into two sulfydryls, which could in turn adhere to the surface of CdSe/ZnS QDs to form a nanotheranostic system. This novel system exhibited good controlled drug release behaviour due to the ester linkage between the drug and polymer. By using typical cancer cell lines, we showed that this nanotheranostic system is promising in cancer diagnosis and treatment in vitro.

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Formulation, characterization, and in vitro evaluation of quantum dots loaded in poly(lactide)-vitamin E TPGS nanoparticles for cellular and molecular imaging

Biotechnology and Bioengineering ◽

10.1002/bit.21924 ◽

2008 ◽

Vol 101 (3) ◽

pp. 622-633 ◽

Cited By ~ 31

Author(s):

Jie Pan ◽

Yan Wang ◽

Si-Shen Feng

Keyword(s):

Quantum Dots ◽

Vitamin E ◽

Molecular Imaging ◽

In Vitro Evaluation ◽

Vitamin E Tpgs

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Preparation, characterization and in vitro evaluation of ε-polylysine-loaded polymer blend microparticles for potential pancreatic cancer therapy

Journal of Microencapsulation ◽

10.1080/02652048.2017.1370028 ◽

2017 ◽

Vol 34 (6) ◽

pp. 582-591 ◽

Cited By ~ 2

Author(s):

Merari T. Chevalier ◽

Mónica C. García ◽

Daniela Gonzalez ◽

Sandro M. Gomes-Filho ◽

Daniela S. Bassères ◽

...

Keyword(s):

Pancreatic Cancer ◽

Cancer Therapy ◽

Polymer Blend ◽

In Vitro Evaluation ◽

Pancreatic Cancer Therapy

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Synthesis and in vitro evaluation of a hyaluronic acid–quantum dots–melphalan conjugate

Carbohydrate Polymers ◽

10.1016/j.carbpol.2014.12.057 ◽

2015 ◽

Vol 121 ◽

pp. 132-139 ◽

Cited By ~ 10

Author(s):

Haixing Xu ◽

Jingbo He ◽

Yu Zhang ◽

Lihong Fan ◽

Yaqiong Zhao ◽

...

Keyword(s):

Quantum Dots ◽

Hyaluronic Acid ◽

In Vitro Evaluation

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Amino-Functionalized Silica Nanoparticles: In Vitro Evaluation for Targeted Delivery and Therapy of Pancreatic Cancer

Journal of Nanotechnology ◽

10.1155/2013/768724 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 19

Author(s):

Abbey Y. Kardys ◽

Dhruba J. Bharali ◽

Shaker A. Mousa

Keyword(s):

Pancreatic Cancer ◽

Silica Nanoparticles ◽

Targeted Delivery ◽

Functionalized Silica ◽

In Vitro Evaluation ◽

Pancreatic Cancer Cells ◽

Free Doxorubicin ◽

Preferential Uptake ◽

Functionalized Silica Nanoparticles

We report a method of synthesis and optimization of amino-functionalized silica nanoparticles (SiNPs) and their in vitro evaluation as targeted delivery vehicles for the potential treatment of pancreatic cancer. SiNPs can efficiently encapsulate doxorubicin and can be attached to a targeting moiety such as anti-Claudin-4 (CLN4). The preferential uptake in pancreatic cancer cells, where CLN4 is overexpressed, of SiNPs when conjugated to CLN4 antibody (compared to nonconjugated SiNPs) was confirmed by confocal microscopy. SiNPs encapsulating doxorubicin had greater efficacy in MTT assays than free doxorubicin, and when conjugated to CLN4, the efficacy was dramatically increased (at 1 μM). No apparent carrier toxicity was observed when void SiNPs were used. SiNPs carrying a chemotherapeutic drug have the potential to be used as a targeted therapy for lethal cancers, such as pancreatic cancer. Also, incorporation of fluorescent probes in these SiNPs creates the possibility of their use as an imaging probe for diagnostic purposes.

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