Nitric Oxide and Oral Diseases: Can We Talk About It?

The aim of this study was to investigate comparatively the concentration of a nitrogen radical, particularly nitric oxide (NO)/nitrite (NO2 -), in the tongue-coating and mixed saliva obtained from smokers/non-smokers and drinkers/non-drinkers, proving the cytotoxic and cytoprotective effect of these free radicals in oral metabolism and physiophatolology of oral diseases. Masticatory (stimulated) methods were used in order to determine the salivary flow rates of 100 patients. The tongue-coating was obtained through of tongue cleaning named Kolbe’s cleaner. Nitrite contents was measured spectophotometricaly by Griess method. Despite the NO been admittedly an important mediator of some physiological functions, when it is generated in excess it acts as the molecule-key of the cellular damage and tissue injury. The results point out that the association tobacco/alcohol points positive correlation between the increasing concentration of nitrogen radicals in the mixed saliva in order to favour tobacco/alcohol users, in comparison with the control group (not smokers/not drinkers). However, the NO/NO2 - concentration in tongue-coating is worthless, comparing to mixed saliva.

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The role of nitric oxide in oral diseases

Archives of Oral Biology ◽

10.1016/s0003-9969(02)00183-8 ◽

2003 ◽

Vol 48 (2) ◽

pp. 93-100 ◽

Cited By ~ 54

Author(s):

P.A Brennan ◽

G.J Thomas ◽

J.D Langdon

Keyword(s):

Nitric Oxide ◽

Oral Diseases

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Tongue-coating and mixed saliva obtained from smokers and/or drinkers: nitric oxide/nitrite contents

Cadernos UniFOA ◽

10.47385/cadunifoa.v1i1.1075 ◽

2017 ◽

Vol 1 (1) ◽

pp. 121

Author(s):

Marise Ramos de Souza ◽

Marcelo Genestra

Keyword(s):

Nitric Oxide ◽

Tissue Injury ◽

Salivary Flow ◽

Cellular Damage ◽

Control Group ◽

Tongue Coating ◽

Oral Diseases ◽

Tongue Cleaning ◽

Nitrogen Radicals ◽

Mixed Saliva

The aim of this study was to investigate comparatively the concentration of a nitrogen radical, particularly nitric oxide (NO)/nitrite (NO2 -), in the tongue-coating and mixed saliva obtained from smokers/non-smokers and drinkers/non-drinkers, proving the cytotoxic and cytoprotective effect of these free radicals in oral metabolism and physiophatolology of oral diseases. Masticatory (stimulated) methods were used in order to determine the salivary flow rates of 100 patients. The tongue-coating was obtained through of tongue cleaning named Kolbe’s cleaner. Nitrite contents was measured spectophotometricaly by Griess method. Despite the NO been admittedly an important mediator of some physiological functions, when it is generated in excess it acts as the molecule-key of the cellular damage and tissue injury. The results point out that the association tobacco/alcohol points positive correlation between the increasing concentration of nitrogen radicals in the mixed saliva in order to favour tobacco/alcohol users, in comparison with the control group (not smokers/not drinkers). However, the NO/NO2 - concentration in tongue-coating is worthless, comparing to mixed saliva.

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Ethanol Extract from Astilbe Chinensis Inflorescence Suppresses Inflammation in Macrophages and Growth of Key Oral Pathogenic Bacteria

10.21203/rs.3.rs-900522/v1 ◽

2021 ◽

Author(s):

Jong Min Han ◽

Ina Yun ◽

Kyung Mi Yang ◽

Hye-Sung Kim ◽

Young-Youn Kim ◽

...

Keyword(s):

Nitric Oxide ◽

Pathogenic Bacteria ◽

Inflammatory Responses ◽

Ethanol Extract ◽

Oral Diseases ◽

Protein Levels ◽

Flowering Season ◽

Dental Diseases ◽

Anti Inflammatory ◽

Oxide Synthase

Abstract Dysregulation of infection-derived inflammatory responses has been one of the crucial pathological causes of oral diseases. Even though the organic extracts of Astilbe chinensis have been frequently reported to have anti-inflammatory activity, the study on the extract of A. chinensis inflorescence has yet to be reported. Here, we evaluated the anti-inflammatory efficacy of A. chinensis collected from a variety of regions and seasons and successfully demonstrated that GA-13-6, an ethanol extract of A. chinensis inflorescence collected in a flowering season, inhibited the production of inflammatory mediators and proinflammatory cytokines, such as nitric oxide (NO), tumor necrosis factor (TNF), and interleukin-6 (IL-6) and suppressed the expression of cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS) both in mRNA and protein levels in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Importantly, we for the first time confirmed that GA-13-6 efficiently inhibited the growth of Porphyromonas gingivalis, Streptococcus sanguinis, and Streptococcus mutans, showing that GA-13-6 possesses antibacterial activity against these pivotal oral pathogens. Thus, GA-13-6 is a potential active ingredient not only for the treatment or prevention of periodontal and dental diseases but many other inflammation-related diseases.

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Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166397 ◽

1996 ◽

Vol 54 ◽

pp. 786-787

Author(s):

Chi-Ming Wei ◽

Margarita Bracamonte ◽

Shi-Wen Jiang ◽

Richard C. Daly ◽

Christopher G.A. McGregor ◽

...

Keyword(s):

Nitric Oxide ◽

Heart Failure ◽

Nitric Oxide Synthase ◽

Endothelial Nitric Oxide Synthase ◽

Cardiac Tissues ◽

Mammalian Tissues ◽

End Stage Heart Failure ◽

End Stage ◽

Oxide Synthase ◽

Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

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