Evaluation of the antidepressant-like effect of chronic administration of Nigella fixed oil versus fluoxetine in rats

2021 ◽  
Vol 20 ◽  
Author(s):  
Youness Kadil ◽  
Imane Tabyaoui ◽  
Latifa Badre ◽  
Nadia Tahiri Jouti ◽  
Houda Filali
Keyword(s):  
Nigella Sativa ◽  
Forced Swim Test ◽  
Chronic Mild Stress ◽  
Chronic Administration ◽  
Histopathological Analysis ◽  
Mild Stress ◽  
Behavioral Test ◽  
Histological Changes ◽  
Swim Test ◽  
Therapeutic Properties

Background: Depression is a group of syndromes characterized by notable and persistent mood disorders, and is one of the most prevalent psychiatric disorders, while the existing treatments have an altered risk-benefit balance. The therapeutic properties of Nigella have been confirmed, suggesting the reliance on phytotherapy. The objective of the present paper is to investigate the antidepressive-like effect of Nigella sativa on rats exposed to the Unpredictable Chronic Mild Stress procedure. Methods: Wistar rats were used to investigate the antidepressive-like effect. The stress procedure used in this study combined many stressful conditions. After 6 weeks of treatment, behavioral test (forced swim test) was conducted, and histological changes of the hippocampus were examined. Results: Treatment by nigella and fluoxetine significantly reduced the struggling time. Conclusion: Histopathological analysis showed that control treatments result in more loosely arranged cells, significant apoptotic neurons characterized by an irregular appearance, and pyknotic hyperchromatic. A reduction of the thickness of the pyramid layer was also observed in the groups treated with nigella and fluoxetine, suggesting that nigella could be used as a treatment or an adjuvant preventing depressive-like disorders.

scholarly journals The behavioral and neurochemical aspects of the interaction between antidepressants and unpredictable chronic mild stress

Acta Naturae ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 63-72
Author(s):  
Nikita V. Kudryashov ◽  
T. C. Kalinina ◽  
A. A. Shimshirt ◽  
A. V. Volkova ◽  
V. B. Narkevich ◽  
...  
Keyword(s):  
Single Injection ◽  
Forced Swim Test ◽  
Chronic Mild Stress ◽  
Chronic Administration ◽  
Mild Stress ◽  
Swim Test ◽  
Forced Swim ◽  
Adaptive Mechanisms ◽  
Outbred Mice ◽  
Stress Dependent

The behavioral and neurochemical effects of amitriptyline (10 mg/kg, i.p.) and fluoxetine (20 mg/kg, i.p.) after single and chronic administration in the setting of unpredictable mild stress in outbred mice ICR (CD-1) were studied. After 28-day exposure to stress we observed an increase in depressive reactions in forced swim test in mice and decrease in serotonin (5-HT) and 5-oxyindol acetic acid (5-HIAA) levels in hippocampus, an increase in noradrenaline (NA) concentration in hypothalamus. Single and chronic administration of amitriptyline and fluoxetine led to shortening of immobilization and increase in swimming in forced swim test. The antidepressant-like effect of fluoxetine, but not of amitriptyline, after single injection coincided with an increase of 5-HT turnover in hippocampus. The chronic administration of the antidepressants caused an increase in NA levels in hypothalamus. Thus, antidepressant-like effect of amitriptyline and fluoxetine may arise from enhancement of stress-dependent adaptive mechanisms depleted by chronic stress.

scholarly journals Studies of the effect of cerebroprotective substances on the course of stress-induced behavioral depression

2018 ◽  
Vol 4 (3) ◽  
pp. 43-48
Author(s):  
Tamara O. Zaika ◽  
Dmitriy V. Evdokimov ◽  
Igor I. Abramets
Keyword(s):  
Hippocampal Neurons ◽  
Sucrose Solution ◽  
Forced Swim Test ◽  
Hippocampal Slices ◽  
Chronic Administration ◽  
Behavioral Experiments ◽  
Behavioral Depression ◽  
Swim Test ◽  
Cerebroprotective Activity

Introduction. Atrophic disturbances of neurons of the limbic structures of the brain, which lead to insufficient regulation of emotions and mood, cause depression. Substances with cerebroprotective activity have the ability to inhibit further development and even reverse atrophic damage to neurons. Materials and methods. Using electrophysiological techniques, the cerebroprotective activity of piracetam, diacamf – (±)-cis-3-(2-benzimidazolyl)-1,2,2-trimethylcyclopentanone-carboxylic acid hydrochloride and the compound R-86, or 3,2’-spiro-pyrrolo-2-oxindole, was investigated in rat hippocampal slices. In behavioral experiments, there was studied the influence of the above substances, which had been administered for 20 days, on the most important manifestations of behavioral depression in rats caused by a five-day swim stress, such as the time of immobilization in the forced swim test and the indicator of preference for consuming sucrose solution. In addition, the influence of piracetam and diacamf was studied on the effects of the classic antidepressant imipramine. Results and discussion. It was found that piracetam, diacamf and the compound R-86 in in vitro studies reduced the damage to the pyramidal hippocampal neurons caused by anoxia and aglycemia, the excitotoxic activity of N-methyl-D-aspartate and oxidative stress when hydrogen peroxide was applied to the slices. Cerebroprotective activity of the test substances, when they are systemically administered for 20 days, is linked with their antidepressant-like effect, which was manifested in a decrease in the immobilization time in the swim test and an increase in the sucrose solution consumption indicator. Co-administration of piracetam in rats potentiated antidepressant activity of imipramine, and diacamf showed additive synergism with the antidepressant. Conclusion. Substances with cerebroprotective activity in their chronic administration may show an antidepressant-like effect. Those that potentiate the action of classical anidepressants can be used in conjunction with antidepressants during episodes of exacerbation of the disease. Less active cerebroprotective drugs can be recommended during remission for its prolongation.

scholarly journals PERUBAHAN PERILAKU GROOMING DAN IMOBILITAS MENCIT BALB/C TERINDUKSI DEPRESI YANG DISUPLEMENTASI TEMPE SEBAGAI SUMBER PARAPROBIOTIK

2020 ◽  
Vol 14 (01) ◽  
pp. 1
Author(s):  
Vanessa Ardianty ◽  
Brian Saputra Manurung
Keyword(s):  
Forced Swim Test ◽  
Chronic Mild Stress ◽  
Tail Suspension Test ◽  
Depression Symptoms ◽  
Depression Symptom ◽  
Mild Stress ◽  
Immobility Time ◽  
Depression Level ◽  
The Common ◽  
Probiotic Food

Depression, a mental disorder marked by sadness, anhedonia and increased fatigability, is becoming more common in this industry 4.0 era. Nowadays, depression affects approximately 322 million people around the world (more than 14 million in Indonesia) and gives major contribution to the rise of global burden of disease. Although antidepressant is considered the common treatment for depression, recent studies show a possibility to treat depression by altering gut microbiome of the patient, by giving them probiotic food. Tempeh is a globally well-known Indonesian paraprobiotic food which already proven to modulate gut microbiota. This research aimed to find out the effect of tempeh to depression symptom as expressed in Balb/c mice behaviors. The methods used was to feed tempeh or tempeh starter to mice which were depressed-induced by Unpredictable Chronic Mild Stress (UCMS) procedure. Parameter measured were depression level of the Balb/c mice based on immobility times and grooming durations acquired from tail suspension test, forced swim test, sucrose splash test and coat state score. The result showed that tempeh supplementation did not affect coat states and grooming durations but tend to improve immobility times of the Balb/c mice. Meanwhile, tempeh starter supplementation tends to improve the immobility time and kept coat state clean/tidy, but lowered grooming duration. In conclusion, supplementation of paraprobiotic tempeh, especially in starter form, tend to improve depression symptoms. Keywords: depression, mice, paraprobiotic, tempeh

Standardisation and Validation of Acute and Chronic Administration Ketamine at Different Doses to Produce Psychosis Like Behavioural Changes in Mice

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Monu Yadav ◽  
Milind Parle ◽  
Mamta Sachdeva ◽  
Sameer Dhingra
Keyword(s):  
Passive Avoidance ◽  
Treatment Period ◽  
Forced Swim Test ◽  
Chronic Administration ◽  
Passive Avoidance Test ◽  
Latency Time ◽  
Behavioural Changes ◽  
Avoidance Test ◽  
Swim Test ◽  
Step Down

Psychosis is a complex mental illness, characterised by positive, negative and cognitive symptoms. NMDA receptor antagonists have been established to induce behavioural as well as biochemical changes in rodents similar to psychotic patients. The aim of the present study was to investigate the effective dose and treatment period of ketamine to induce some behavioural changes. The results suggest that acute treatment of ketamine (50 and 100 mg/kg, i.p.) induced hyperlocomotor activity and reduced step down latency time in passive avoidance test, whereas in effective in forced swim test. Further, with the chronic administration of ketamine (50 and 100 mg/kg, i.p.) effective to produced hyperlocomotor activity, reduced the step down latency time in passive avoidance test and enhanced the immobility duration in forced swim test. Moreover, these behavioural changes persisted for 7 days after the treatment period. Thus, our findings suggest that the chronic administration of Ketamine (50 and 100 mg/kg, i.p.) potential to produce behavioural changes, would serve as an effective tool to screen antipsychotic drugs.

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