Antihyperglycemic, Antihyperlipidemic and Antioxidant Effects of Cotula cinerea (Del) in Normal and Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 (9) ◽  
pp. 1504-1513 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Glucose Tolerance ◽  
Oral Administration ◽  
Beneficial Effect ◽  
Aqueous Extract ◽  
Aerial Part ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Phytochemical Screening ◽  
Cotula Cinerea

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.

Beneficial Effect of Saharan Propolis on Glucose Metabolism in Streptozotocin-induced Diabetic Rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Mourad Akdad ◽  
Azzoui Bouchra ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Serum Lipid ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Phytochemical Screening ◽  
Antihyperglycemic Effect ◽  
Desert Region ◽  
Beneficial Action ◽  
Ancient Times

Aims: The aim of the study was to evaluate the antihyperglycemic activity of propolis from Saharan region of Morocco. Background: Propolis is known from ancient times for its beneficial action on health. Objective: The study aimed to evaluate the effect of Moroccan propolis aqueous extract from the desert region on glycemia and lipid metabolism. Methods: The antihyperglycemic and antidyslipidemic activities of Moroccan propolis aqueous extract were evaluated in streptozotocin-induced diabetic rats. Glycemia was monitored during acute (6h) and subchronic treatments. Histopathological analysis of the liver and the serum lipid profile was also evaluated in addition to the glucose tolerance. Results: This work demonstrated that the aqueous extract of Moroccan propolis exhibited a significant antihyperglycemic effect in diabetic rats. Histopathological data demonstrated that propolis acts positively on the liver of diabetic rats. In addition, the preliminary phytochemical screening revealed that Moroccan propolis contains several phytochemicals. Finally, Moroccan propolis had a potent antioxidant activity which may be due to the richness in polyphenols content (90.91 mg EAG/1 g of extract). Conclusion: The present study shows that Moroccan propolis from the hot desert region possesses a potent antihyperglycemic effect.

scholarly journals Leaf Aqueous Extract of Argania spiniosa Exhibits Antihyperglycemic Effect in Diabetic Rats

2019 ◽  
Vol 17 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Morad Hebi ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Argania Spinosa ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Leaf Aqueous Extract

Introduction: Argania Spinosa L. (Sapotaceae) is an endemic species from south-western Morocco. This plant has many traditional uses including its use in the treatment of diabetes. Objective: The objective of the study was to evaluate the antidiabetic activity of Argania Spinosa Leaf Aqueous Extract (A.S.L.A.E). Methods: The antidiabetic effect of A.S.L.A.E was evaluated in both normal and streptozotocin (STZ)-induced diabetic rats treated at a dose of 20 mg/kg body weight for 15 days. The histopathological changes in the liver were evaluated. In addition, the antioxidant activity of this extract was also studied. Results: Single oral administration of A.S.L.A.E (20 mg/kg) showed no significant change in blood glucose levels in both normal and STZ induced diabetic rats after 6 hours of administration. Furthermore, in normal rats, repeated oral administration of A.S.L.A.E reduced blood glucose levels. Moreover, blood glucose levels decreased in STZ diabetic rats after fifteen days of treatment. According to the oral glucose tolerance test, the A.S.L.A.E (20 mg/kg) was shown to prevent significantly the increase in blood glucose levels in normal treated rats. Moreover, A.S.L.A.E showed antioxidant activity. Conclusion: The results show that Argania spinosa leaf aqueous extract possesses significant antihyperglycemic activity.

Antihyperglycemic Effect of the Moroccan Collard Green (Brassica oleracea var. viridis) in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Brassica Oleracea ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Antihyperglycemic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Collard Green

Aims: The aim of the study was to assess the antihyperglycemic activity of Brassica oleracea. Background: Collard green or Brassica oleracea var. viridis which belongs to Brassicaceae family is ranked at 10th place of “powerhouse” vegetables, and it is commonly used traditionally for the treatment of diabetes in Morocco. Objective: This current investigation aimed to assess the antihyperglycemic capacity of the aqueous extract of Collard green leaves. Methods: The effect of a single (6 hours) and repeated (seven days of treatment) oral administration of Collard green aqueous extract (CGAE) at a dose of 60 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of liver was carried out according to the Hematoxylin-Eosin method. Furthermore, a preliminary phytochemical screening, and the quantification of phenolic, flavonoid and tannins contents as well as the antioxidant activity using DPPH assay were carried out. Results: The results demonstrated that Collard green A.E. (CGAE) exhibited a significant antihyperglycemic effect and positive improvement liver histology in diabetic rats. After a single oral administration of CGAE, blood glucose levels were lowered from 15±2 mM to 4.9±1 mM (p<0.0001) at the sixth hour in diabetic rats but no change was observed in normal rats. Repeated CGAE administration was able to reduce blood glucose levels from 15±2 mM to normal values (5±1 mM at the seventh day, p<0.0001) while the extract had no effect in normal rats. In addition, we revealed that CGAE is rich in several phytochemical compounds and exerts an important antioxidant activity. Conclusion: In conclusion, this study demonstrates that Collard green possesses a beneficial effect against diabetes, and can be used in the program diet of diabetic patients.

scholarly journals Effect of Terebinthus atlanticus on Glucose Metabolism in Diabetic Rats

2020 ◽  
Vol 20 (1) ◽  
pp. 31-40
Author(s):  
Fadwa El-Ouady ◽  
Lhoussaine Hajji ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Control Group ◽  
Histopathological Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background:: Terebinthus atlanticus (Anacardiaceae) is an important source of essential oil and phenolic compounds justifying its use in traditional medicine. Objective:: The present work aimed to evaluate the antidiabetic and the antioxidant activities of the aqueous extract of the leaves of Terebinthus atlanticus (T. atlanticus). Methods:: The current study evaluated the effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of the leaves of T. atlanticus (PALAE) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rats. Furthermore, the effect of PALAE on glucose tolerance and histopathological examination of the liver was carried out. Results:: A single oral administration of PALAE reduced blood glucose levels in normal (p<0.05), and STZ diabetic rats (p<0.0001), 6 and 4 hours after administration, respectively. Furthermore, this extract had an optimal effect (p<0.0001) in both normal and STZ diabetic rats at the 15th and 7th day of treatment. This extract was also shown to prevent significantly the increase on blood glucose levels 120 min after glucose administration, in both normal (p<0.05), and diabetic (p<0.01) treated rats when compared to the control group. In addition, the histopathological analysis highlighted the positive effect of T. atlanticus on pancreas and liver. Conclusion:: The study demonstrates the antihyperglycemic effect of the aqueous T. atlanticus extracts in diabetic rats which should be mediated through the amelioration of the oxidative stress as well as an improvement in liver histology.

Aqueous Asteriscus graveolens Extract Exhibits Antidiabetic and Hepatoprotective Effects in Diabetic Rats

2020 ◽  
Vol 10 (4) ◽  
pp. 459-466
Author(s):  
Fadwa El-Ouady ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Aerial Part ◽  
Diabetic Rats ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
In Vitro Antioxidant Activity

Background: Asteriscus graveolens (A. graveolens) is a medicinal plant with numerous applications in phytotherapy including diabetes management. However, the antidiabetic effect of this plant has never been investigated experimentally. Objective: The objective of this study was to evaluate the antidiabetic effect of aqueous extract prepared from the aerial part of Asteriscus graveolens (A. graveolens) in normal and STZ-induced diabetic rats. Method: The effect of a single dose and daily oral administration for 15 days of the aerial part aqueous extract of A. graveolens (AGAPE) (10 mg/kg) on blood glucose levels was evaluated. In addition, histopathological examination of the liver as well as the in vitro antioxidant activity AGAPE were performed. Results: The data illustrate that both single and repeated oral administration of AGAPE were able to reduce blood glucose levels in normal and diabetic rats. The extract ameliorated histopathological properties of liver in diabetic rats and it exhibited in vitro antioxidant activity. Conclusion: In conclusion, the present investigation revealed for the first time that A. graveolens possesses potent antidiabetic and hepatoprotective activities which support the traditional claim of the plant.

Antihyperglycemic Activity of Aqueous Extract of Euphorbia guyoniana in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Ayoub Amssayef ◽  
Bouchra Azzaoui ◽  
Ismail Bouadid ◽  
Mohamed Eddouks
Keyword(s):  
Oral Administration ◽  
Aqueous Extract ◽  
Extensor Digitorum Longus ◽  
Glycogen Content ◽  
Diabetic Rats ◽  
Extensor Digitorum ◽  
Histopathological Analysis ◽  
Glycogen Depletion ◽  
Traditional Use

Aims: This work assessed the antihyperglycemic effect of Euphorbia guyoniana. Background: Euphorbia guyoniana (Boss. and Reut.) is widely used in traditional medicine. Objective: This study was designed to confirm this traditional use by assessing its antihyperglycemic capacity in vivo. Methods: The effect of the aqueous extract of Euphorbia guyoniana (Boss. and Reut.) (60 mg/kg) on glycemia in both normal and diabetic rats was evaluated. The glycogen content in the liver and skeletal muscles (extensor digitorum longus and soleus) was measured. Furthermore, liver histopathological analysis was performed. Results: The findings showed that Euphorbia guyoniana (Boss. and Reut.) exhibited a significant decrease in glycaemia in diabetic rats (from 20±2 mmol/l to 5.5 mmol/l after 6 hours of oral administration; p<0.0001 and from 20±2 mmol/l to 4.5 mmol/l after 7 days of once-daily repeated oral administration of the aqueous Euphorbia guyoniana extract; p<0.0001). In addition, the extract increased the glycogen content in the liver (41±4 mg/g versus 70±5 mg/g in normal and diabetic rats respectively) and extensor digitorum longus (39±4 mg/g versus 60±1 mg/g in normal and diabetic rats, respectively), and partially restored corporal weight in diabetic rats. Furthermore, this aqueous extract has been shown to suppress hyperglycemia induced by glucose load in treated diabetic rats. Additionally, hepatic histology in diabetic rats has been improved. This plant revealed the presence of several phytochemical constituents and possessed antioxidant activity. Conclusion: The current study evidenced that Euphorbia guyoniana (Boss. and Reut.) has a beneficial effect on improving hyperglycemia and glycogen depletion in the diabetic state.

Evaluation of the Anti-Hypercholesterolemic and Antioxidant Activity of Mentha pulegium (L.) Aqueous Extract in Normal and Streptozotocin- Induced Diabetic Rats

2020 ◽  
Vol 10 (3) ◽  
pp. 236-243
Author(s):  
Omar Farid ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Plasma Cholesterol ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Scavenging Activity ◽  
Mentha Pulegium

Objective:Mentha pulegium L., an aromatic plant belonging to Lamiaceae family, is widely used by local population against diabetes, hypertension and cardiovascular disorders. The present study aimed to evaluate the in vivo antihypercholesterolemic effect of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) in normal and streptozotocin-induced diabetic rat. Additionally, the in vitro antioxidant activity of M. pulegium A.P.A.E has been estimated.Methods:The effect of M. pulegium (L.) A.P.A.E (20 mg of lyophilized A.P.A.E per kg body weight) on plasma lipid profile was investigated in normal and streptozotocin (STZ) diabetic rats (n=6) treated for 15 days with M. pulegium (L.) A.P.A.E oral administration. The antioxidant activity was evaluated using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity. Estimation of total polyphenol contents in A.P.A.E of M. pulegium was determined with the Folin- Ciocalteu reagent by the method using gallic acid as a standard phenolic compound. Also, flavonoids were estimated according to the method based on the formation of a complex flavonoidaluminium.Results:The results show that 15 days of M. pulegium A.P.A.E oral administration alleviated hyperlipidemia in diabetic rats by lowering significantly (p<0.01) the plasma cholesterol levels without affecting the triglycerides (TG) levels significantly. However, no significant decrease in plasma cholesterol and plasma triglycerides has been shown in diabetic control rats. In addition, A.P.A.E oral administration has exerted some increasing activity on plasma HDL-c level (54.84 %), whereas the HDL-c level, in diabetic control rats, has significantly decreased (p<0.05). On the other hand, M. pulegium A.P.A.E showed a high amount of flavonoid (83.07 ± 0.58 mg EQ / g of extract) and phenolic compounds (239.08 ± 35.40 mg EAG/g of extract). Also, according to the DPPH radical scavenging activity, this aqueous extract has demonstrated a significant antioxidant activity.Conclusion:We conclude that 15 days of M. pulegium A.P.A.E oral administration exhibited a significant antihypercholesterolemic effect and has also demonstrated a not negligent increase in HDL-c levels without affecting the triglycerides levels. Furthermore, A.P.A.E exhibited a potent antioxidant activity. Therefore, phytochemical compounds and antioxidant activity of M. pulegium A.P.A.E may be seemingly implicated in the antihypercholesterolemic effect demonstrated in this study.

Mentha pulegium Aqueous Extract Exhibits Antidiabetic and Hepatoprotective Effects in Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (3) ◽  
pp. 292-301
Author(s):  
Omar Farid ◽  
Naoufel Ali Zeggwagh ◽  
Fadwa EL Ouadi ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Aqueous Extract ◽  
Morphometric Analysis ◽  
Diabetic Rats ◽  
Mentha Pulegium ◽  
Glucose Levels ◽  
Histological Sections ◽  
Blood Glucose Levels ◽  
Liver And Pancreas

Objective: The aim of this work was to evaluate the antihyperglycemic activity of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rat. The glucose tolerance was evaluated in normal rats. Moreover, the histological sections and morphometric analysis at the liver and pancreas have been carried out in this investigation both in normal and STZ-diabetic rats. Methods: The effect of A.P.A.E of M. pulegium (20 mg/kg) on blood glucose levels was investigated in normal and diabetic rats (n=6). Histopathological changes in liver and pancreas were examined under phase contrast microscope and a preliminary screening for various bioactive constituents was realized according to standard methods. Key Findings: Both single and repeated oral administration of A.P.A.E (20 mg/kg) caused a significant reduction in blood glucose levels in STZ-diabetic rats (p<0.0001). The morphometric analysis and histological sections realized in pancreas and liver have showed the beneficial effect of the A.P.A.E in cellular population. According to oral glucose tolerance test (OGTT), the aqueous extract has revealed an improvement of glucose tolerance in normal rat. Furthermore, the preliminary phytochemical screening of A.P.A.E of M. pulegium has demonstrated the presence of various metabolite compounds including polyphenols, flavonoids, terpenoids tannins, cyanidins, sesquiterpenes, and glycosides. Conclusion: We conclude that the A.P.A.E of M. pulegium (20 mg/kg) exhibits a potent antihyperglycemic activity in STZ diabetic rats.

Chenopodium quinoa Exhibits Antihyperglycemic Activity in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Vol 19 ◽  
Author(s):  
Amine Azzane ◽  
Ayou Amssayef ◽  
Mohame Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Chenopodium Quinoa ◽  
Diabetic Rats ◽  
Favorable Effect ◽  
Antihyperglycemic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The aim of the study was to evaluate the antihyperglycemic effect of Chenopodium quinoa. Background: Chenopodium quinoa is a pseudocereal plant with several medicinal properties. Objective: The goal of this investigation was to determine the antihyperglycemic activity of Chenopodium quinoa in both normal and streptozotocin(STZ)-induced diabetic rats. Methods: In this study, the effect of the aqueous extract of Chenopodium quinoa seeds (AECQS) (60 mg/kg) on blood glucose levels was evaluated in both normal and diabetic rats after a single (6 hours) and repeated oral administration (7 days of treatment). The effect of this herb on glucose tolerance and lipid profile was also studied. Additionally, histopathological examination of liver was carried out using the Hematoxylin-Eosin method. Furthermore, the in vitro antioxidant activity as well as a preliminary phytochemical screening and quantification of some secondary metabolites (phenolic compounds, flavonoids and tannins) were performed according to standard methods. Results: AECQS produced a significant lowering effect on plasma glucose levels in STZ-induced diabetic rats. In addition, this extract exhibited a remarkable amelioration on hepatic histopathology in diabetic rats. In addition, the extract exerted a remarkable antioxidant activity which could be due to the presence of some compounds found in this herb. Conclusion: In conclusion, this study demonstrates that the aqueous extract of Chenopodium quinoa seeds has a favorable effect in controlling diabetes mellitus.

scholarly journals Physiological study to investigate the activity of an aqueous extract ofCinnamomum cassiabark on the blood glucose levels in healthy and diabetic rats induced by streptozotocin (stz)

2016 ◽  
Vol 13 (4) ◽  
pp. 681-693
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Physiological Study ◽  
Cinnamomum Cassia ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Oral Administration

The present study was investigated the activity of aqueous extract from Cinnamomum cassia bark on the blood glucose levels in healthy and diabetic rats induced by Streptozotcin (STZ). In healthy rats the blood glucose levels were slightly decreased after six hoursof single oral administration with dose (25 mg/kg) of body wight, as well as four weeks after twice daily repeated oral administration of aqueous extract of Cinnamomum cassia bark. In streptozotocin induced diabetic rats we absorved high significant decreased (p

Sign in / Sign up

Export Citation Format

Share Document