Beneficial Effect of Saharan Propolis on Glucose Metabolism in Streptozotocin-induced Diabetic Rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Mourad Akdad ◽  
Azzoui Bouchra ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Serum Lipid ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Phytochemical Screening ◽  
Antihyperglycemic Effect ◽  
Desert Region ◽  
Beneficial Action ◽  
Ancient Times

Aims: The aim of the study was to evaluate the antihyperglycemic activity of propolis from Saharan region of Morocco. Background: Propolis is known from ancient times for its beneficial action on health. Objective: The study aimed to evaluate the effect of Moroccan propolis aqueous extract from the desert region on glycemia and lipid metabolism. Methods: The antihyperglycemic and antidyslipidemic activities of Moroccan propolis aqueous extract were evaluated in streptozotocin-induced diabetic rats. Glycemia was monitored during acute (6h) and subchronic treatments. Histopathological analysis of the liver and the serum lipid profile was also evaluated in addition to the glucose tolerance. Results: This work demonstrated that the aqueous extract of Moroccan propolis exhibited a significant antihyperglycemic effect in diabetic rats. Histopathological data demonstrated that propolis acts positively on the liver of diabetic rats. In addition, the preliminary phytochemical screening revealed that Moroccan propolis contains several phytochemicals. Finally, Moroccan propolis had a potent antioxidant activity which may be due to the richness in polyphenols content (90.91 mg EAG/1 g of extract). Conclusion: The present study shows that Moroccan propolis from the hot desert region possesses a potent antihyperglycemic effect.

Antihyperglycemic, Antihyperlipidemic and Antioxidant Effects of Cotula cinerea (Del) in Normal and Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 (9) ◽  
pp. 1504-1513 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Glucose Tolerance ◽  
Oral Administration ◽  
Beneficial Effect ◽  
Aqueous Extract ◽  
Aerial Part ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Phytochemical Screening ◽  
Cotula Cinerea

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.

Antihyperglycemic Effect of the Moroccan Collard Green (Brassica oleracea var. viridis) in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Brassica Oleracea ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Antihyperglycemic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Collard Green

Aims: The aim of the study was to assess the antihyperglycemic activity of Brassica oleracea. Background: Collard green or Brassica oleracea var. viridis which belongs to Brassicaceae family is ranked at 10th place of “powerhouse” vegetables, and it is commonly used traditionally for the treatment of diabetes in Morocco. Objective: This current investigation aimed to assess the antihyperglycemic capacity of the aqueous extract of Collard green leaves. Methods: The effect of a single (6 hours) and repeated (seven days of treatment) oral administration of Collard green aqueous extract (CGAE) at a dose of 60 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of liver was carried out according to the Hematoxylin-Eosin method. Furthermore, a preliminary phytochemical screening, and the quantification of phenolic, flavonoid and tannins contents as well as the antioxidant activity using DPPH assay were carried out. Results: The results demonstrated that Collard green A.E. (CGAE) exhibited a significant antihyperglycemic effect and positive improvement liver histology in diabetic rats. After a single oral administration of CGAE, blood glucose levels were lowered from 15±2 mM to 4.9±1 mM (p<0.0001) at the sixth hour in diabetic rats but no change was observed in normal rats. Repeated CGAE administration was able to reduce blood glucose levels from 15±2 mM to normal values (5±1 mM at the seventh day, p<0.0001) while the extract had no effect in normal rats. In addition, we revealed that CGAE is rich in several phytochemical compounds and exerts an important antioxidant activity. Conclusion: In conclusion, this study demonstrates that Collard green possesses a beneficial effect against diabetes, and can be used in the program diet of diabetic patients.

Acute toxicity Analysis and Antidiabetic Effect of the Moroccan Spider Flower (Cleome Arabica L.) in Normal and Sreptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Antihyperglycemic Effect ◽  
Toxicity Analysis ◽  
Cleome Arabica ◽  
In Vitro Antioxidant Activity

Aims: The study aimed to assess the antihyperglycemic effect of Cleome arabica. Background: Cleome arabica L. or spider flower belongs to Capparidaceae family and it is used for treating inflammation and diabetes mellitus in traditional medicine. Objective: This study aimed to evaluate the antihyperglycemic activity and acute toxicity of the aqueous extract of Cleome arabica L (CAAE). Methods: The acute toxicity of CAAE was evaluated at the doses of 500, 1000, or 2000 mg/kg. Parallelly, body weight, signs of toxicity, and/or mortality were observed for 14 days. The effect of oral administration of Cleome arabica aqueous extract (CAAE) at a dose of 100 mg/kg on glycemia was performed in normal and diabetic rats. Additionally, histopathological structure of liver, phytochemical screening and in vitro antioxidant activity were studied. Results: The acute toxicity test revealed that all treated rats survived, and no change in body weight was observed. The results demonstrated that CAAE exhibited significant antihyperglycemic effect in diabetic rats. Furthermore, the plant extract ameliorated the liver histology in diabetic rats with a concomitant antioxidant activity.

Chenopodium quinoa Exhibits Antihyperglycemic Activity in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Vol 19 ◽  
Author(s):  
Amine Azzane ◽  
Ayou Amssayef ◽  
Mohame Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Chenopodium Quinoa ◽  
Diabetic Rats ◽  
Favorable Effect ◽  
Antihyperglycemic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The aim of the study was to evaluate the antihyperglycemic effect of Chenopodium quinoa. Background: Chenopodium quinoa is a pseudocereal plant with several medicinal properties. Objective: The goal of this investigation was to determine the antihyperglycemic activity of Chenopodium quinoa in both normal and streptozotocin(STZ)-induced diabetic rats. Methods: In this study, the effect of the aqueous extract of Chenopodium quinoa seeds (AECQS) (60 mg/kg) on blood glucose levels was evaluated in both normal and diabetic rats after a single (6 hours) and repeated oral administration (7 days of treatment). The effect of this herb on glucose tolerance and lipid profile was also studied. Additionally, histopathological examination of liver was carried out using the Hematoxylin-Eosin method. Furthermore, the in vitro antioxidant activity as well as a preliminary phytochemical screening and quantification of some secondary metabolites (phenolic compounds, flavonoids and tannins) were performed according to standard methods. Results: AECQS produced a significant lowering effect on plasma glucose levels in STZ-induced diabetic rats. In addition, this extract exhibited a remarkable amelioration on hepatic histopathology in diabetic rats. In addition, the extract exerted a remarkable antioxidant activity which could be due to the presence of some compounds found in this herb. Conclusion: In conclusion, this study demonstrates that the aqueous extract of Chenopodium quinoa seeds has a favorable effect in controlling diabetes mellitus.

scholarly journals Leaf Aqueous Extract of Argania spiniosa Exhibits Antihyperglycemic Effect in Diabetic Rats

2019 ◽  
Vol 17 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Morad Hebi ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Argania Spinosa ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Leaf Aqueous Extract

Introduction: Argania Spinosa L. (Sapotaceae) is an endemic species from south-western Morocco. This plant has many traditional uses including its use in the treatment of diabetes. Objective: The objective of the study was to evaluate the antidiabetic activity of Argania Spinosa Leaf Aqueous Extract (A.S.L.A.E). Methods: The antidiabetic effect of A.S.L.A.E was evaluated in both normal and streptozotocin (STZ)-induced diabetic rats treated at a dose of 20 mg/kg body weight for 15 days. The histopathological changes in the liver were evaluated. In addition, the antioxidant activity of this extract was also studied. Results: Single oral administration of A.S.L.A.E (20 mg/kg) showed no significant change in blood glucose levels in both normal and STZ induced diabetic rats after 6 hours of administration. Furthermore, in normal rats, repeated oral administration of A.S.L.A.E reduced blood glucose levels. Moreover, blood glucose levels decreased in STZ diabetic rats after fifteen days of treatment. According to the oral glucose tolerance test, the A.S.L.A.E (20 mg/kg) was shown to prevent significantly the increase in blood glucose levels in normal treated rats. Moreover, A.S.L.A.E showed antioxidant activity. Conclusion: The results show that Argania spinosa leaf aqueous extract possesses significant antihyperglycemic activity.

Potent Antihyperglycemic Effects of an Endemic Plant from Morocco (Matthiola maroccana Coss.) in Normal and Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Nadia Lahrach ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Endemic Plant ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The study aims to evaluate the antidiabetic effect of Matthiola maroccana (Coss.). Background: Matthiola maroccana (Coss.) belongs to Brassicaceae family is an endemic plant from Morocco. Objective: The objective of the study was to evaluate the effect of its aqueous extract on blood glucose levels in normal and diabetic rats. Method: Single dose (6 hours) and daily oral administration for seven days of the Aerial Part Aqueous Extract (A.P.A.E) of Matthiola maroccana (Coss.) (M. maroccana) at a dose of 20 mg/kg body weight on blood glucose levels both in normal and streptozotocin(STZ)-induced diabetic rats. Furthermore, body weight, oral glucose tolerance test, liver histopathological examination, phytochemical screening, and in vitro antioxidant activity of A.P.A.E were performed in this study. Results: The results showed that M. maroccana A.P.A.E exerts potent hypoglycemic and antihyperglycemic effects in normal and STZ-induced diabetic rats (p<0.0001). Also, it was able to restore body weight in diabetic rats (p<0.05). Furthermore, the aqueous extract has been shown to regenerate hepatic tissues in diabetic rats. In addition, A.P.A.E revealed the presence of several phytochemical constituents (polyphenols, flavonoids, tannins, saponins, alkaloids, sterols and terpenoids), and possessed an antioxidant activity. Conclusion: In conclusion, our findings showed that A.P.A.E of M. maroccana (A.P.A.E MM) possesses a significant antihyperglycemic and hypoglycemic activity.

Assessment of Antihyperglycemic Effect and Acute Toxicity of the Aqueous Scorzonera undulata Extract in Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammed Ajebli ◽  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Glycogen Content ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Antihyperglycemic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Phytochemical Investigation

Aims: The current study was carried out in order to assess the antihyperglycemic effect of Scorzonera undulata (S. undulata). Backgroud: Scorzonera undulata subsp. deliciosa (DC.) Maire is used in the Moroccan pharmacopeia for managing diabetes. Objective: The objective of the study was to evaluate the anithyperglycemic activity in normal and streptozotocin (STZ)- induced diabetic rats as well as the acute toxicity of the aqueous extract of the roots of Scorzonera undulata (S. undulata). Methods: This study investigated the effects of the root aqueous extract of Scorzonera undulata (AERSU) at a dose of 20 mg/kg on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rats and the acute toxicity of AERSU was examined. Histopathological examination, preliminary phytochemical investigation, determination of glycogen content and evaluation of α-amylase were also performed. Result: Both single and repeated oral doses of AERSU (20 mg/kg) produced a significant reduction of blood glucose levels in normal and diabetic rats. Furthermore, repeated oral administration of AERSU during 15 days increased the glycogen content in the liver in both normal and diabetic rats, inhibited α-amylase activity and improved the histological architecture of the liver and pancreas in treated diabetic rats and ameliorated some biochemical parameters such as ALT and AST. In addition, the preliminary phytochemical investigation showed the richness of the roots of S. undulata in certain phytochemicals particularly in polyphenols. Conclusion: AERSU exhibits an evident antihyperglycemic activity. This pharmacological effect may be due at least to protection of surviving pancreatic β cells, to the protection of hepatocytes, to α-amylase inhibitory effect and to the improvement of glycogen storage in the liver. The dose used in this study seems to be free of any toxicity.

A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera

2021 ◽  
Vol 3 (2) ◽  
pp. 43
Author(s):  
Awalul Fatiqin ◽  
Hanif Amrulloh ◽  
Ike Apriani ◽  
Aneke Lestari ◽  
Berta Erawanti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Leaf Part ◽  
Different Parts

In this work, we reported a comparative study on the phytochemical screening and antioxidant activity of aqueous extract from different parts of Moringa oleifera plant, i.e. leaves, twigs, stem barks, and woods. The phytochemical content of each extract was screened through qualitative analysis and followed by quantitative analysis for the determination of total phenolic content and total flavonoid content. The results showed that different parts of Moringa oleifera plant would give different extracted natural products. Either leaf or twig part of Moringa oleifera contained alkaloids, flavonoids, saponins, carbohydrates, polyphenols, proteins, amino acids, and phenolics. Meanwhile, only flavonoids, carbohydrates, and phenolics were found in the aqueous extract of the wood part of Moringa oleifera. In agreement to the phytochemical assay, the aqueous extract of leaf part of Moringa oleifera gave the highest total phenolic content (684 ± 16.7 µg GAE mL-1) and the highest flavonoid content (514 ± 26.1 µg CE mL-1) than other parts. Consequently, the leaf extract exhibited the highest antioxidant activity (64.6 ± 0.69%) which is close to the antioxidant activity of ascorbic acid (71.7 ± 0.56%) as the positive control. This finding is critical for the utilization of Moringa oleifera leaf part as the most potent source of antioxidant agents in the future.

Sign in / Sign up

Export Citation Format

Share Document