scholarly journals Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

2018 ◽  
Vol 12 (1) ◽  
pp. 123-134 ◽  
Author(s):  
Munawwar A. Khan ◽  
Poojabahen G. Patel ◽  
Arpitha G. Ganesh ◽  
Naushad Rais ◽  
Sultan M. Faheem ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
United Arab Emirates ◽  
Methane Production ◽  
Archaeal Community ◽  
Full Scale ◽  
Anaerobic Sludge ◽  
Fish Analysis ◽  
Anaerobic Digesters ◽  
Archaeal Communities

Introduction:Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescencein situhybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique.Methods & Materials:Fluorescencein situhybridization (FISH) was performed to detect and quantify the methanogenicArchaeain the sludge samples whereas qPCR was carried out to support the FISH analysis. Multiple probes targeting domain archaea, different orders and families of Archaea were used for the studies.Results and Discussion:In general, the aceticlastic organisms(Methanosarcinaceae & Methanosaetaceae)were more abundant than the hydrogenotrophic organisms(Methanobacteriales, Methanomicrobiales, Methanobacteriaceae & Methanococcales). Both FISH and qPCR indicated that familyMethanosaetaceaewas the most abundant suggesting that aceticlastic methanogenesis is probably the dominant methane production pathway in these digesters.Conclusion:Future work involving high-throughput sequencing methods and correlating archaeal communities with the main operational parameters of anaerobic digesters will help to obtain a better understanding of the dynamics of the methanogenic archaeal community in wastewater treatment plants in United Arab Emirates (UAE) which in turn would lead to improved performance of anaerobic sludge digesters.

Elevational characteristics of the archaeal community in full-scale activated sludge wastewater treatment plants at a 3,660-meter elevational scale

2017 ◽  
Vol 76 (3) ◽  
pp. 531-541 ◽  
Author(s):  
Lihua Niu ◽  
Xue Zhang ◽  
Yi Li ◽  
Peifang Wang ◽  
Wenlong Zhang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Archaeal Community ◽  
Full Scale ◽  
Anaerobic Reactors ◽  
Treatment Processes ◽  
Operational Variables ◽  
First Time ◽  
Archaeal Communities

Due to the important roles of archaea in wastewater treatment processes, archaeal communities have been studied extensively in various anaerobic reactors, but the knowledge of archaeal communities in full-scale activated sludge wastewater treatment plants (WWTPs) remains quite poor. In this study, 454-pyrosequencing was for the first time employed to investigate archaeal communities from 20 full-scale activated sludge WWTPs distributed at a 3,660-meter elevational scale in China. Results showed that archaeal communities from WWTPs were dominated by Methanosarcinales (84.6%). A core archaeal population (94.5%) composed of Methanosaeta, Methanosarcina, Methanogenium and Methanobrevibacter was shared among WWTPs. The elevational pattern of archaeal communities was observed in WWTPs, with an elevational threshold associated with archaeal community richness and structures at approximately 1,500 meters above sea level (masl). A declining trend in community richness with increasing elevation was observed at higher elevations, whereas no trend was presented at lower elevations. Spearman correlation analysis indicated that the archaeal community richness at higher elevations was associated with more environmental variables than that at lower elevations. Redundancy analysis indicated that wastewater variables were the dominant contributors to the variation of community structures at higher elevations, followed by operational variables and elevation.

scholarly journals Microbiota of anaerobic digesters in a full-scale wastewater treatment plant

2017 ◽  
Vol 43 (3) ◽  
pp. 53-60 ◽  
Author(s):  
Piotr Świątczak ◽  
Agnieszka Cydzik-Kwiatkowska ◽  
Paulina Rusanowska
Keyword(s):  
Microbial Community ◽  
Sewage Sludge ◽  
Methane Production ◽  
Treatment Plant ◽  
Full Scale ◽  
Successful Implementation ◽  
Anaerobic Digesters ◽  
Syntrophic Bacteria ◽  
The Stability ◽  
Microbial Groups

AbstractAnaerobic digestion is an important technology for the bio-based economy. The stability of the process is crucial for its successful implementation and depends on the structure and functional stability of the microbial community. In this study, the total microbial community was analyzed during mesophilic fermentation of sewage sludge in full-scale digesters.The digesters operated at 34–35°C, and a mixture of primary and excess sludge at a ratio of 2:1 was added to the digesters at 550 m3/d, for a sludge load of 0.054 m3/(m3·d). The amount and composition of biogas were determined. The microbial structure of the biomass from the digesters was investigated with use of next-generation sequencing.The percentage of methanogens in the biomass reached 21%, resulting in high quality biogas (over 61% methane content). The abundance of syntrophic bacteria was 4.47%, and stable methane production occurred at a Methanomicrobia to Synergistia ratio of 4.6:1.0. The two most numerous genera of methanogens (about 11% total) wereMethanosaetaandMethanolinea, indicating that, at the low substrate loading in the digester, the acetoclastic and hydrogenotrophic paths of methane production were equally important. The high abundance of the orderBacteroidetes, including the classCytophagia(11.6% of all sequences), indicated the high potential of the biomass for efficient degradation of lignocellulitic substances, and for degradation of protein and amino acids to acetate and ammonia.This study sheds light on the ecology of microbial groups that are involved in mesophilic fermentation in mature, stably-performing microbiota in full-scale reactors fed with sewage sludge under low substrate loading.

scholarly journals In situ visualisation of the abundant Chloroflexi populations in full-scale anaerobic digesters and the fate of immigrating species

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206255 ◽  
Author(s):  
Francesca Petriglieri ◽  
Marta Nierychlo ◽  
Per Halkjær Nielsen ◽  
Simon Jon McIlroy
Keyword(s):  
Full Scale ◽  
Anaerobic Digesters

Application of the IWA Anaerobic Digestion Model (ADM1) for simulating full-scale anaerobic sewage sludge digestion

2005 ◽  
Vol 52 (1-2) ◽  
pp. 487-492 ◽  
Author(s):  
Y. Shang ◽  
B.R. Johnson ◽  
R. Sieger
Keyword(s):  
Wastewater Treatment ◽  
Steady State ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Treatment Plant ◽  
Biochemical Parameter ◽  
Full Scale ◽  
Anaerobic Digesters ◽  
Biogas Yield ◽  
Volatile Solids

A steady-state implementation of the IWA Anaerobic Digestion Model No. 1 (ADM1) has been applied to the anaerobic digesters in two wastewater treatment plants. The two plants have a wastewater treatment capacity of 76,000 and 820,000 m3/day, respectively, with approximately 12 and 205 dry metric tons sludge fed to digesters per day. The main purpose of this study is to compare the ADM1 model results with full-scale anaerobic digestion performance. For both plants, the prediction of the steady-state ADM1 implementation using the suggested physico-chemical and biochemical parameter values was able to reflect the results from the actual digester operations to a reasonable degree of accuracy on all parameters. The predicted total solids (TS) and volatile solids (VS) concentration in the digested biosolids, as well as the digester volatile solids destruction (VSD), biogas production and biogas yield are within 10% of the actual digester data. This study demonstrated that the ADM1 is a powerful tool for predicting the steady-state behaviour of anaerobic digesters treating sewage sludges. In addition, it showed that the use of a whole wastewater treatment plant simulator for fractionating the digester influent into the ADM1 input parameters was successful.

Biological Treatment of High Ammonium Content Urban Landfill Leachate Using an Anaerobic-Aerobic Process

2013 ◽  
Vol 781-784 ◽  
pp. 2095-2099
Author(s):  
Hong Wei Sun ◽  
Yong Jun You ◽  
Ying Guo
Keyword(s):  
Landfill Leachate ◽  
Inhibitory Effect ◽  
Nitrifying Bacteria ◽  
Anaerobic Sludge ◽  
Nitrite Accumulation ◽  
Fish Analysis ◽  
Free Nitrous Acid ◽  
Nitrite Oxidizing Bacteria ◽  
Anaerobic Sludge Blanket

Biological system consisting of an up-flow anaerobic sludge blanket (UASB) and anoxic-oxic (A/O) reactor was applied to treat high ammonium content urban landfill leachate. Inhibitory effect of free ammonia (FA) and free nitrous acid (FNA) on nitrifying bacteria activity was used to achieve nitrogen removal via nitrite pathway in the A/O. Results demonstrated that removed efficiencies of COD, total nitrogen (TN) and NH4+-N were 95.3%, 84.6 %and 99.2%, respectively. Stable nitrite pathway with above 90% nitrite accumulation was successfully achieved in the A/O reactor by synergetic inhibition of FA and FNA on the activity of nitrite oxidizing bacteria (NOB). Moreover, Fluorescence in situ hybridization (FISH) analysis showed that AOB was dominant microorganism.

New Technologies for Anaerobic Wastewater Treatment

1986 ◽  
Vol 18 (12) ◽  
pp. 41-53 ◽  
Author(s):  
Look Hulshoff Pol ◽  
Gatze Lettinga
Keyword(s):  
Wastewater Treatment ◽  
New Technologies ◽  
Anaerobic Treatment ◽  
Full Scale ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Anaerobic Wastewater Treatment ◽  
Start Up ◽  
Uasb Reactors ◽  
Sludge Granulation

Presently anaerobic wastewater treatment is becoming an accepted simple technology for the treatment of a variety of wastewaters. Of the different treatment systems that have been developed the UASB process (Upflow Anaerobic Sludge Blanket) has found the widest application. Almost all of the more than 60 full scale UASB reactors in operation now, are running satisfactorily. The excellent sludge retention generally found in UASB-reactors is obtained by sludge granulation, which can be seen as a sludge immobilization process. The presently available insight into the sludge granulation process is briefly presented, together with the strategy to be applied for performing a proper first start-up and secondary start-up of UASB reactors, viz. using granular seed sludge. The effect of the presence of SS with regard to the loading potentials of anaerobic treatment systems will be discussed. The experiences obtained with some full scale applications of the UASB-process are presented.

scholarly journals Environmental performance of a full-scale wastewater treatment plant applying Life Cycle Assessment

2018 ◽  
Vol 13 (4) ◽  
pp. 1 ◽  
Author(s):  
Thaís Andrade de Sampaio Lopes ◽  
Luciano Matos Queiroz ◽  
Asher Kiperstok
Keyword(s):  
Wastewater Treatment ◽  
Life Cycle Assessment ◽  
Life Cycle ◽  
Environmental Impacts ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Anaerobic Sludge ◽  
Treated Effluent ◽  
Useful Life

Life Cycle Assessment (LCA) was applied to estimate and analyze the environmental impacts from the construction and operation phases of a full-scale wastewater treatment plant (WWTP) located in the municipality of Lauro de Freitas, Bahia, Brazil. The WWTP process consists of the association of an Upflow Anaerobic Sludge Blanket (UASB) reactor followed by four constructed wetlands (CWL) and a disinfection step. The functional unit was defined as one cubic meter of treated effluent during the useful life of this WWTP (20 years). The LCA was carried out using SimaPro® software and the Centre of Environmental Science (CML) assessment method. The environmental impacts during construction phase were mainly from the wooden forms for concrete and the use of reinforcing steel. During the operation phase, the chlorine used as effluent disinfectant caused the greatest impacts in the abiotic depletion and acidification categories. Macronutrient concentrations present in the treated effluent and the methane generated also caused significant environmental impacts during the WWTP’s useful life. The results obtained highlight the importance of the application of a methodology like LCA to assist in decision-making with regard to the implementation, construction and operation of a WWTP.

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