scholarly journals In situ visualisation of the abundant Chloroflexi populations in full-scale anaerobic digesters and the fate of immigrating species

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206255 ◽  
Author(s):  
Francesca Petriglieri ◽  
Marta Nierychlo ◽  
Per Halkjær Nielsen ◽  
Simon Jon McIlroy
Keyword(s):  
Full Scale ◽  
Anaerobic Digesters

scholarly journals Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

2018 ◽  
Vol 12 (1) ◽  
pp. 123-134 ◽  
Author(s):  
Munawwar A. Khan ◽  
Poojabahen G. Patel ◽  
Arpitha G. Ganesh ◽  
Naushad Rais ◽  
Sultan M. Faheem ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
United Arab Emirates ◽  
Methane Production ◽  
Archaeal Community ◽  
Full Scale ◽  
Anaerobic Sludge ◽  
Fish Analysis ◽  
Anaerobic Digesters ◽  
Archaeal Communities

Introduction:Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescencein situhybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique.Methods & Materials:Fluorescencein situhybridization (FISH) was performed to detect and quantify the methanogenicArchaeain the sludge samples whereas qPCR was carried out to support the FISH analysis. Multiple probes targeting domain archaea, different orders and families of Archaea were used for the studies.Results and Discussion:In general, the aceticlastic organisms(Methanosarcinaceae & Methanosaetaceae)were more abundant than the hydrogenotrophic organisms(Methanobacteriales, Methanomicrobiales, Methanobacteriaceae & Methanococcales). Both FISH and qPCR indicated that familyMethanosaetaceaewas the most abundant suggesting that aceticlastic methanogenesis is probably the dominant methane production pathway in these digesters.Conclusion:Future work involving high-throughput sequencing methods and correlating archaeal communities with the main operational parameters of anaerobic digesters will help to obtain a better understanding of the dynamics of the methanogenic archaeal community in wastewater treatment plants in United Arab Emirates (UAE) which in turn would lead to improved performance of anaerobic sludge digesters.

scholarly journals Upscaling THM modeling from small-scale to full-scale in-situ experiments in the Callovo-Oxfordian claystone

2021 ◽  
Vol 144 ◽  
pp. 104582
Author(s):  
D.M. Seyedi ◽  
C. Plúa ◽  
M. Vitel ◽  
G. Armand ◽  
J. Rutqvist ◽  
...  
Keyword(s):  
Full Scale ◽  
Small Scale ◽  
In Situ Experiments

Investigation of planing craft maneuverability using full-scale tests

2021 ◽  
pp. 147509022110303
Author(s):  
Kazem Sadati ◽  
Hamid Zeraatgar ◽  
Aliasghar Moghaddas
Keyword(s):  
Full Scale ◽  
Performance Characteristics ◽  
Forward Speed ◽  
Speed Reduction ◽  
Planing Craft ◽  
Full Scale Tests ◽  
Turning Maneuver

Maneuverability of planing craft is a complicated hydrodynamic subject that needs more studies to comprehend its characteristics. Planing craft drivers follow a common practice for maneuver of the craft that is fundamentally different from ship’s standards. In situ full-scale tests are normally necessary to understand the maneuverability characteristics of planing craft. In this paper, a study has been conducted to illustrate maneuverability characteristics of planing craft by full-scale tests. Accelerating and turning maneuver tests are conducted on two cases at different forward speeds and rudder angles. In each test, dynamic trim, trajectory, speed, roll of the craft are recorded. The tests are performed in planing mode, semi-planing mode, and transition between planing mode to semi-planing mode to study the effects of the craft forward speed and consequently running attitude on the maneuverability. Analysis of the data reveals that the Steady Turning Diameter (STD) of the planing craft may be as large as 40 L, while it rarely goes beyond 5 L for ships. Results also show that a turning maneuver starting at planing mode might end in semi-planing mode. This transition can remarkably improve the performance characteristics of the planing craft’s maneuverability. Therefore, an alternative practice is proposed instead of the classic turning maneuver. In this practice, the craft traveling in the planing mode is transitioned to the semi-planing mode by forward speed reduction first, and then the turning maneuver is executed.

scholarly journals Anaerobic Digestion and Removal of Sulfamethoxazole, Enrofloxacin, Ciprofloxacin and Their Antibiotic Resistance Genes in a Full-Scale Biogas Plant

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 502
Author(s):  
Andrea Visca ◽  
Anna Barra Caracciolo ◽  
Paola Grenni ◽  
Luisa Patrolecco ◽  
Jasmin Rauseo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Emerging Contaminants ◽  
Antibiotic Resistance Genes ◽  
Animal Manure ◽  
Full Scale ◽  
Antibiotic Residues ◽  
Anaerobic Digesters ◽  
One Year

Anaerobic digestion is one of the best ways to re-use animal manure and agricultural residues, through the production of combustible biogas and digestate. However, the use of antibiotics for preventing and treating animal diseases and, consequently, their residual concentrations in manure, could introduce them into anaerobic digesters. If the digestate is applied as a soil fertilizer, antibiotic residues and/or their corresponding antibiotic resistance genes (ARGs) could reach soil ecosystems. This work investigated three common soil emerging contaminants, i.e., sulfamethoxazole (SMX), ciprofloxacin (CIP), enrofloxacin (ENR), their ARGs sul1, sul2, qnrS, qepA, aac-(6′)-Ib-cr and the mobile genetic element intI1, for one year in a full scale anaerobic plant. Six samplings were performed in line with the 45-day hydraulic retention time (HRT) of the anaerobic plant, by collecting input and output samples. The overall results show both antibiotics and ARGs decreased during the anaerobic digestion process. In particular, SMX was degraded by up to 100%, ENR up to 84% and CIP up to 92%, depending on the sampling time. In a similar way, all ARGs declined significantly (up to 80%) in the digestate samples. This work shows how anaerobic digestion can be a promising practice for lowering antibiotic residues and ARGs in soil.

Coupled Thermo-Hydro-Geochemical Models of Engineered Barrier Systems: The Febex Project

2000 ◽  
Vol 663 ◽  
Author(s):  
J. Samper ◽  
R. Juncosa ◽  
V. Navarro ◽  
J. Delgado ◽  
L. Montenegro ◽  
...  
Keyword(s):  
Large Scale ◽  
Reference Model ◽  
Numerical Models ◽  
Full Scale ◽  
Small Scale ◽  
Model Parameters ◽  
In Situ Test ◽  
Wide Range ◽  
Engineered Barrier

ABSTRACTFEBEX (Full-scale Engineered Barrier EXperiment) is a demonstration and research project dealing with the bentonite engineered barrier designed for sealing and containment of waste in a high level radioactive waste repository (HLWR). It includes two main experiments: an situ full-scale test performed at Grimsel (GTS) and a mock-up test operating since February 1997 at CIEMAT facilities in Madrid (Spain) [1,2,3]. One of the objectives of FEBEX is the development and testing of conceptual and numerical models for the thermal, hydrodynamic, and geochemical (THG) processes expected to take place in engineered clay barriers. A significant improvement in coupled THG modeling of the clay barrier has been achieved both in terms of a better understanding of THG processes and more sophisticated THG computer codes. The ability of these models to reproduce the observed THG patterns in a wide range of THG conditions enhances the confidence in their prediction capabilities. Numerical THG models of heating and hydration experiments performed on small-scale lab cells provide excellent results for temperatures, water inflow and final water content in the cells [3]. Calculated concentrations at the end of the experiments reproduce most of the patterns of measured data. In general, the fit of concentrations of dissolved species is better than that of exchanged cations. These models were later used to simulate the evolution of the large-scale experiments (in situ and mock-up). Some thermo-hydrodynamic hypotheses and bentonite parameters were slightly revised during TH calibration of the mock-up test. The results of the reference model reproduce simultaneously the observed water inflows and bentonite temperatures and relative humidities. Although the model is highly sensitive to one-at-a-time variations in model parameters, the possibility of parameter combinations leading to similar fits cannot be precluded. The TH model of the “in situ” test is based on the same bentonite TH parameters and assumptions as for the “mock-up” test. Granite parameters were slightly modified during the calibration process in order to reproduce the observed thermal and hydrodynamic evolution. The reference model captures properly relative humidities and temperatures in the bentonite [3]. It also reproduces the observed spatial distribution of water pressures and temperatures in the granite. Once calibrated the TH aspects of the model, predictions of the THG evolution of both tests were performed. Data from the dismantling of the in situ test, which is planned for the summer of 2001, will provide a unique opportunity to test and validate current THG models of the EBS.

scholarly journals Microbiota of anaerobic digesters in a full-scale wastewater treatment plant

2017 ◽  
Vol 43 (3) ◽  
pp. 53-60 ◽  
Author(s):  
Piotr Świątczak ◽  
Agnieszka Cydzik-Kwiatkowska ◽  
Paulina Rusanowska
Keyword(s):  
Microbial Community ◽  
Sewage Sludge ◽  
Methane Production ◽  
Treatment Plant ◽  
Full Scale ◽  
Successful Implementation ◽  
Anaerobic Digesters ◽  
Syntrophic Bacteria ◽  
The Stability ◽  
Microbial Groups

AbstractAnaerobic digestion is an important technology for the bio-based economy. The stability of the process is crucial for its successful implementation and depends on the structure and functional stability of the microbial community. In this study, the total microbial community was analyzed during mesophilic fermentation of sewage sludge in full-scale digesters.The digesters operated at 34–35°C, and a mixture of primary and excess sludge at a ratio of 2:1 was added to the digesters at 550 m3/d, for a sludge load of 0.054 m3/(m3·d). The amount and composition of biogas were determined. The microbial structure of the biomass from the digesters was investigated with use of next-generation sequencing.The percentage of methanogens in the biomass reached 21%, resulting in high quality biogas (over 61% methane content). The abundance of syntrophic bacteria was 4.47%, and stable methane production occurred at a Methanomicrobia to Synergistia ratio of 4.6:1.0. The two most numerous genera of methanogens (about 11% total) wereMethanosaetaandMethanolinea, indicating that, at the low substrate loading in the digester, the acetoclastic and hydrogenotrophic paths of methane production were equally important. The high abundance of the orderBacteroidetes, including the classCytophagia(11.6% of all sequences), indicated the high potential of the biomass for efficient degradation of lignocellulitic substances, and for degradation of protein and amino acids to acetate and ammonia.This study sheds light on the ecology of microbial groups that are involved in mesophilic fermentation in mature, stably-performing microbiota in full-scale reactors fed with sewage sludge under low substrate loading.

scholarly journals The impact of immigration on microbial community composition in full-scale anaerobic digesters

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rasmus H. Kirkegaard ◽  
Simon J. McIlroy ◽  
Jannie M. Kristensen ◽  
Marta Nierychlo ◽  
Søren M. Karst ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Composition ◽  
Microbial Community Composition ◽  
Full Scale ◽  
Anaerobic Digesters ◽  
The Impact

Research on in situ continuous off-line chemical cleaning in full-scale membrane bioreactors

2018 ◽  
Vol 4 ◽  
pp. 186-192 ◽  
Author(s):  
Liwei Liu ◽  
Jin Tian ◽  
Chunhui Luo ◽  
Chunsheng Chen ◽  
Jicheng Liu ◽  
...  
Keyword(s):  
Membrane Bioreactors ◽  
Full Scale ◽  
Chemical Cleaning

Development of a Field Instrument to Quantify Frictional Drag of an In-Service Ship Hull

2015 ◽  
Author(s):  
J. Travis Hunsucker ◽  
Geoffrey Swain
Keyword(s):  
19Th Century ◽  
Detrimental Effect ◽  
Experimental Testing ◽  
Channel Length ◽  
Full Scale ◽  
Frictional Drag ◽  
Late 19Th Century ◽  
Marine Fouling ◽  
Accurate Quantification

It has been shown that the presence of marine fouling, even as a light slime, will cause a detrimental effect on the powering or speed of a full-scale ship. Studies from as early as the late 19th century have attempted to quantify the increase in power or decrease in speed imposed on a ship from the presence of hull roughness. The accurate quantification is limited and often difficult and expensive to obtain. The present study aims to develop an instrument that will remove some ambiguity by directly measuring the frictional drag of a ship in situ. Results from experimental testing of a prototype in the lab are presented and used to identify the channel length, height, and accuracy limitations of a field deployable prototype.

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