Crocin protects against beta-amyloid peptide-induced apoptosis in PC12 cells via PI3 K pathway

2020 ◽  
Vol 13 ◽  
Author(s):  
Reyhaneh Taheri ◽  
Elham Hadipour ◽  
Zahra Tayarani-Najaran
Keyword(s):  
Pc12 Cells ◽  
Caspase 3 ◽  
Beta Amyloid ◽  
Pi3 Kinase ◽  
Amyloid Peptide ◽  
Ros Generation ◽  
Protective Effects ◽  
Beta Amyloid Peptide ◽  
Cyt C ◽  
Induced Apoptosis

Background: Crocin is a known compound with antioxidant and anti-inflammatory property which many help to reduce the progression of neurological disorders. In this study, we aimed to investigate the protective effects of crocin on beta-amyloid peptide Aβ (1-40) and hydrogen peroxide (H2O2) induced neurotoxicity in PC12 cells. Methods: PC12 cells pretreated with crocin and donepezil (5 and 10 µM) for 2 h then treated with Aβ (1-40) (25 µM) for 24 h. In parallel after pretreatment with crocin (5 and 10 µM) and donepezil (5 and 10 µM) for 24 h, cells were treated with H2O2 (800 µM) for 4 h. Finally, the cell viability and intracellular reactive oxygen species (ROS) generation were evaluated using AlamarBlue® and 2', 7'-dichlorodihydrofluorescein diacetate (DCFH-DA), respectively. The western blot test was done to compare the protein level of phospho SAPK/JNK, SAPK/JNK, PI3 Kinase P85, Phospho-PI3 Kinase P85, caspase-3 and cytochrome c )cyt c). Results: Crocin and donepezil could significantly decrease the Aβ toxicity and ROS level. While treatment with Aβ increased Cyt c release from mitochondria to cytosol, cleaved form of caspase-3 (17 kDa) and activated form of SAPK/JNK p44/4 and decreased the activated form of PI3 Kinase P85 protein, crocin could significantly block the apoptosis initiated with Aβ. Conclusions: According to the results crocin could be a promising candidate for further evaluations against the development of Alzheimer's diseases through mitogen-activated protein kinases (MAPK) and the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling (PI3 K/AKT) pathways.

scholarly journals Ferulago Angulata Methanolic Extract Protects PC12 Cells Against Beta Amyloid Peptide Induced Toxicity

2021 ◽  
Vol 0 (0) ◽  
pp. 1-22
Author(s):  
Leila Hashemi ◽  
◽  
Maliheh Soodi ◽  
Homa Hajimehdipoor ◽  
Abolfazl Dashti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Medicinal Plants ◽  
Pc12 Cells ◽  
Caspase 3 ◽  
Methanolic Extract ◽  
Beta Amyloid ◽  
Amyloid Peptide ◽  
Neuroprotective Activity ◽  
Ros Production ◽  
Beta Amyloid Peptide

Alzheimer's disease (AD) is an age-dependent neurodegenerative disease. Beta-amyloid peptide (Aβ)- induce neurotoxicity has a pivotal role in AD pathogenesis, therefore modulation of Aβ toxicity is the promising therapeutic approach for control of disease progression. Medicinal plants for having multiple active ingredients are effective in complex diseases such as AD therefore; several studies have been focused on medicinal plants for finding an effective treatment for AD. Ferulago angulata is a medicinal plant with the antioxidant and neuroprotective activity. The present study aims to assess the protective effect of the methanolic extract of Ferulago angulate on Aβ-induced toxicity and oxidative stress in PC12 cells. Methanolic extract of aerial parts of the plant was prepared by maceration method. PC12 cells were cultured according to a standard protocol. PC12 cells were incubated for 24 hours with Aβ alone, and Aβ in combined with various concentrations of the Ferulago angulata extract. Cell viability was determined by the MTT assay. Also, ROS production and the activity of Acetylcholine esterase (AChE), glutathione peroxidase (GPx), and caspase-3 enzymes were measured. The extract dose-dependently protects PC12 cells against Aβ-induced cell death. Also, Aβ increases the ROS production, AChE, and caspase-3 activity and decreases the GPx activity, which all were ameliorated by Ferulago angulata extract. Results of the present study indicate that Ferulago angulata extract protects against Aβ-induced oxidative stress and apoptosis. These effects may be due to the antioxidant and anticholinesterase activity of the extract. It is recommended Ferulago angulata extract be investigated more as an anti-Alzheimer agent.

scholarly journals P1-384: Protective effects of alkaloids from polyalthia glauca on beta-amyloid peptide (1-42)-induced neurotoxicity and caspase-3 in rat cortical cell cultures

2013 ◽  
Vol 9 ◽  
pp. P299-P299
Author(s):  
Wipawan Thangnipon ◽  
Nicha Puangmalai ◽  
Vorapin Chinchalongporn ◽  
Narisorn Kitiyanant ◽  
Patoomratana Tuchinda ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Caspase 3 ◽  
Cortical Cell ◽  
Beta Amyloid ◽  
Amyloid Peptide ◽  
Protective Effects ◽  
Beta Amyloid Peptide ◽  
Cortical Cell Cultures

Genistein inhibits mitochondrial-targeted oxidative damage induced by beta-amyloid peptide 25–35 in PC12 cells

2011 ◽  
Vol 43 (4) ◽  
pp. 399-407 ◽  
Author(s):  
Yuan-Di Xi ◽  
Huan-Ling Yu ◽  
Wei-Wei Ma ◽  
Bing-Jie Ding ◽  
Juan Ding ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Pc12 Cells ◽  
Beta Amyloid ◽  
Amyloid Peptide ◽  
Beta Amyloid Peptide

scholarly journals Neuroprotective Effects of Coreopsis lanceolata Flower Extract against Oxidative Stress-Induced Apoptosis in Neuronal Cells and Mice

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 951
Author(s):  
Hyung Don Kim ◽  
Ji Yeon Lee ◽  
Jeong-Yong Park ◽  
Dong Hwi Kim ◽  
Min Hye Kang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Pc12 Cells ◽  
Caspase 3 ◽  
Antioxidant Activities ◽  
Neuronal Cells ◽  
Water Extract ◽  
Protective Effects ◽  
Neuroprotective Effects ◽  
Induced Apoptosis

(1) Background: Coreopsis lanceolata L. is a perennial plant of the family Asteraceae, and its flower is known to contain flavonoids with various bioactivities. We evaluated the effect of Coreopsis lanceolata L. flower (CLF) extracts on H2O2-induced oxidative stress (OS) in neuronal cells and mouse neurons. (2) Methods: The flowering part of CL was used as CLF1 (70% ethanol extract) and CLF2 (water extract), and 10 types of phenolic compounds were quantified using high-performance liquid chromatography. To evaluate the neuroprotective effects of CLF, the antioxidant activities of the extracts were measured, and the expression levels of antioxidant enzymes and proteins related to OS-induced apoptosis in neuronal cells and mouse neurons treated with the extracts were investigated. (3) Results: In the in vitro study, CLF ameliorated H2O2-induced oxidative stress and induced the expression of antioxidant enzymes in PC12 cells. Furthermore, CLF1 enhanced the expression of the Bcl-xL protein but reduced the expression of Bax and the cleavage of caspase-3. In the same manner, CLF1 showed neuroprotective effects against OS in vivo. Pretreatment with CLF1 (200 mg/kg) increased the Bcl-2 protein and decreased Bax compared with the 1-methyl-4-phenylpyridinium ion (MPP+)-treated C57BL/6 mice model group. Our results suggest that the protective effects of CLF1 on MPP+-induced apoptosis may be due to its anti-apoptotic activity, through regulating the expression of the Bcl-2 family. (4) Conclusions: CLF1 exerts neuroprotective effects against OS-induced apoptosis in PC12 cells in a Parkinson’s disease model mouse. This effect may be attributable to the upregulation of Bcl-2 protein expression, downregulation of Bax expression, and inhibition of caspase-3 activation. These data indicate that CLF may provide therapeutic value for the treatment of progressive neurodegenerative diseases.

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