scholarly journals Bio-hydrogen Production from Beer Wastewater in an Internal Circulation (IC) Reactor

2015 ◽  
Vol 8 (1) ◽  
pp. 81-85
Author(s):  
Su Caili ◽  
Ji baojie ◽  
Wang Qun ◽  
Zhu Lingfeng
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Granular Sludge ◽  
Production Equipment ◽  
Organic Loading Rate ◽  
Internal Circulation ◽  
Fermentation Products ◽  
High Hydrogen ◽  
Fermentative Hydrogen Production ◽  
Liquid Products

An internal circulation (IC) using hydrogen-producing anaerobic granular sludge as seed sludge and beer wastewater as substrate was employed to evaluate the effect of hydrogen production and the performance of reactor. Running at the temperature of 35±1 °C, the pH value of influent controlled is 5.5 and organic loading rate (OLR) from 30 kg COD/(m3●d) to 42 kg COD/(m3●d), the IC reactor presents a high hydrogen production ability as the hydrogen production rate (HPR) maximized at 6.0-6.83 m3/(m3●d). Hydrogen volume content was estimated to be 42-46% of the total biogas and the biogas was free of methane throughout the study. COD removal efficiency could reach 20-30% and the dissolved fermentation products were predominated by ethanol with the concentration of 900-950 mg/L, which accounts for 45%- 56% of the total liquid products. These values may imply that the IC reactor is a kind of feasible fermentative hydrogen production equipment.

Continuous Fermentative Hydrogen Production from Brown Sugar Using EGSB Reactor

2010 ◽  
Vol 113-116 ◽  
pp. 1132-1137 ◽  
Author(s):  
Yong Feng Li ◽  
Yong Ming Hui ◽  
Xin Yao ◽  
Lu Wang ◽  
Qian Wen Song ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Hydraulic Retention Time ◽  
Loading Rate ◽  
Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Hydrogen Production Rate ◽  
Brown Sugar ◽  
Fermentative Hydrogen Production ◽  
Fermentative Hydrogen

In this experiment, brown sugar was chosen as the substrate of continuous operation. A lab-scale expanded granular sludge blanket (EGSB) reactor was employed. Stable ethanol-type fermentation was formed by controlling the organic loading rate (OLR). The results showed a maximum hydrogen production rate of 5.73L / L reactor•d was achieved, under the condition that the hydraulic retention time (HRT) = 2h, OLR = 97.2kg COD/m3 reactor•d. The average hydrogen content in the biogas during the 73-day operation was 41.27%.

scholarly journals Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. M. Silva ◽  
A. A. Abreu ◽  
A. F. Salvador ◽  
M. M. Alves ◽  
I. C. Neves ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Anaerobic Bacteria ◽  
Dark Fermentation ◽  
Inorganic Materials ◽  
Biohydrogen Production ◽  
High Rate ◽  
Continuous Reactor ◽  
Organic Loading Rate ◽  
Fermentation Products ◽  
Zeolite Type

AbstractThermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065–0.26 g g−1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15–21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10–26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g−1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L−1 day−1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m−3 day−1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g−1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L−1 day−1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.

scholarly journals Anaerobic treatment of glycerol for methane and hydrogen production

2013 ◽  
Vol 14 (2) ◽  
pp. 149-156 ◽  
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Anaerobic Treatment ◽  
Microbial Culture ◽  
Batch Reactors ◽  
Continuous Stirred Tank Reactor ◽  
Organic Loading ◽  
Loading Rates ◽  
Fermentative Hydrogen Production ◽  
Initial Ph

This work focused on glycerol exploitation for biogas and hydrogen production. Anaerobic digestion of pure glycerol was studied in a continuous stirred tank reactor (CSTR), operated under mesophilic conditions (35oC) at various organic loading rates. The overall operation of the reactor showed that it could not withstand organic loading rates above 0.25 g COD L-1 d-1, where the maximum biogas (0.42 ± 0.05 L (g COD)-1) and methane (0.30 ± 0.04 L (g COD)-1) production were achieved. Fermentative hydrogen production was carried out in batch reactors under mesophilic conditions (35oC), using heat-pretreated anaerobic microbial culture as inoculum. The effects of initial concentration of glycerol and initial pH value on hydrogen production were studied. The highest yield obtained was 22.14 ± 0.46 mL H2 (g COD added)-1 for an initial pH of 6.5 and an initial glycerol concentration of 8.3 g COD L-1. The main metabolic product was 1.3 propanediol (PDO), while butyric and acetic acids as well as ethanol, at lower concentrations, were also determined.

Anaerobic bio-hydrogen production using pre-heated river sediments as seed sludge

2005 ◽  
Vol 52 (10-11) ◽  
pp. 31-39 ◽  
Author(s):  
J. Zuo ◽  
Y. Zuo ◽  
W. Zhang ◽  
J. Chen
Keyword(s):  
Hydrogen Production ◽  
Hydrogen Content ◽  
Ph Value ◽  
Batch Reactor ◽  
River Sediments ◽  
Organic Loading Rate ◽  
Seed Sludge ◽  
Initial Ph ◽  
Gas Separator ◽  
Different Levels

Anaerobic bio-hydrogen production is the focus in the field of bio-energy resources. In this paper, a series of batch experiments were conducted to investigate the effects of several factors on anaerobic bio-hydrogen producing process carried out by pre-heated river sediments. The results showed that several factors such as substrate and its concentration, temperature and the initial pH value could affect the anaerobic bio-hydrogen production in different levels. At 35°C and the initial pH of 6.5, using glucose of 20,000mg COD/L as substrate, the highest hydrogen production of 323.8ml-H2/g TVS in a 100ml batch reactor was reached, the specific hydrogen production rate was 37.7ml-H2/g TVSh, and the hydrogen content was 51.2%. Thereafter using the same pre-heated river sediments as seed sludge, continuous anaerobic bio-hydrogen production was achieved successfully in a lab-scale CSTR with gas-separator. At the organic loading rate of 36kg COD/m3d, the highest hydrogen production was 6.3–6.7l-H2/l-reactord, the specific hydrogen production was 1.3–1.4mol-H2/mol-glucose, and the hydrogen content in the gas was 52.3%. The effluent of the bio-reactor contained some small molecular organics, mainly including ethanol, acetate, butyrate and their molar proportion is 1 : 1 : 0.6.

scholarly journals Fermentative hydrogen production in packed-bed and packing-free upflow reactors

2006 ◽  
Vol 54 (9) ◽  
pp. 95-103 ◽  
Author(s):  
C. Li ◽  
T. Zhang ◽  
H.H.P. Fang
Keyword(s):  
Hydrogen Production ◽  
Granular Sludge ◽  
Packed Bed ◽  
Synthetic Wastewater ◽  
Extracellular Polysaccharides ◽  
Hydrogen Yield ◽  
Bacterial Cells ◽  
Fermentative Hydrogen Production ◽  
Packing Rings ◽  
Fermentative Hydrogen

Fermentative hydrogen production from a synthetic wastewater containing 10 g/L of sucrose was studied in two upflow reactors at 26°C for 400 days. One reactor was filled with packing rings (RP) and the other was packing free (RF). The effect of hydraulic retention time (HRT) from 2 h to 24 h was investigated. Results showed that, under steady state, the hydrogen production rate significantly increased from 0.63 L/L/d to 5.35 L/L/d in the RF when HRT decreased from 24 h to 2 h; the corresponding rates were 0.56 L/L/d to 6.17 L/L/d for the RP. In the RF, the hydrogen yield increased from 0.96 mol/mol-sucrose at 24 h of HRT to the maximum of 1.10 mol/mol-sucrose at 8 h of HRT, and then decreased to 0.68 mol/mol-sucrose at 2 h. In the RP, the yield increased from 0.86 mol/mol-sucrose at 24 h of HRT to the maximum of 1.22 mol/mol-sucrose at 14 h of HRT, and then decreased to 0.78 mol/mol-sucrose at 2 h. Overall, the reactor with packing was more effective than the one free of packing. In both reactors, sludge agglutinated into granules. The microbial community of granular sludge in RP was investigated using 16S rDNA based techniques. The distribution of bacterial cells and extracellular polysaccharides in hydrogen-producing granules was investigated by fluorescence-based techniques. Results indicated that most of the N-acetyl-galactosamine/galactose-containing extracellular polysaccharides were distributed on the outer layer of the granules with a filamentous structure.

Rapid Start-Up of Ethanol-Type Fermentation in Biological Hydrogen Production Reactor from Molasses Wastewater

2010 ◽  
Vol 113-116 ◽  
pp. 623-631
Author(s):  
Li Ran Yue ◽  
Yong Feng Li ◽  
Wei Han ◽  
Jing Li Xu ◽  
Hong Chen ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Anaerobic Fermentation ◽  
Oxygen Demand ◽  
Low Ph ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Biological Hydrogen Production ◽  
Molasses Wastewater ◽  
Start Up

Research on anaerobic fermentation biohydrogen production from molasses wastewater in a continuous stirred-tank reactor (CSTR) was conducted. Emphasis was focused on the rapid start-up of ethanol-type fermentation in biological hydrogen production reactor. It was found that an initial biomass of 17.71 g/L, temperature of 35°C±1°C, hydraulic retention time (HRT) of 6 h, the reactor could start-up the ethanol-type fermentation at the range of 2000-4000 mg/L and at pH from 3.23 to 4.39 in 12 days with COD (chemical oxygen demand), respectively. The content of hydrogen was 45.77% in the fermentation biogas and the COD removal was 8%. As the hydrogen production system experienced low pH (3.23-4.0), the ethanol-produce bacterial can resume easier compared with other fermentation bacteria which are difficult to restore. In addition, when the pH value ranged from 4.0 to 4.63, the hydrogen production increased with the content of ethanol in liquid fermentation products increased. However, it was detected that the yield of hydrogen decreased with a high content of ethanol in the pH value ranged from 3.23 to 4.0, which demonstrated pH value played the most important role on hydrogen production within low pH.

Control of Start-up and Operation in Continuous Fermentation of Bio-Hydrogen Production Reactor

2011 ◽  
Vol 183-185 ◽  
pp. 552-556
Author(s):  
Zhi Qin ◽  
Dan Qin ◽  
Dan Li
Keyword(s):  
Acetic Acid ◽  
Hydrogen Production ◽  
Butyric Acid ◽  
Ph Value ◽  
Continuous Fermentation ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Biogas Yield ◽  
Acid Type

Bio-hydrogen production from diluted molasses by anaerobic activated sludge was investigated in a continuous stirred-tank reactor (CSTR) under condition of continuous flow in this study. Research shows that the reactor started up under the condition of influent COD concentration 3000mg/L, HRT8h, pH6.5~7.5 and (35±1) °C. The process performed steadily and a dominant butyric acid and acetic acid type fermentation population was established, acetic acid and butyric acid accounted for about 80% in the liquid fermentation products. The effluent PH value was maintained about 5.0. The biogas yield could reach at 4.87L/d while hydrogen yield reached 41.25mL/d under the condition. When influent COD concentration rose to 5500 mg/L, the biogas yield and hydrogen yield as high as 9.45L/d and 119.98mL/d were obtained.

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