Therapeutic Role of Methanolic Extract of Ocimum basilicum L. Seeds and its Isolated Compound as Potent Antidiabetic and Antihyperlipidemic Agents

Background: Ocimum basilicum seed, commonly also known as Takhmaria in Gujarat. The seed of O. basilicum traditionally used to treat diabetes. This activity is related to the presence of flavonoids, the major compounds of the crude extract. Objective: The present study was planned to examine the antidiabetic and antihyperlipidemic potential of Ocimum basilicum Linn seed, used as a traditional treatment for diabetes mellitus. Methods: The methanolic extracts of O. basilicum seed (40 mg/kg) and isolated compound apigenin (10 mg/kg) were administered orally for 15 days to streptozotocin (STZ)-induced diabetic rat. Anti diabetic activity, oral glucose tolerance test, change in body weight and lipid profile of diabetics rat treated with methanolic extracts of O. basilicum seed and isolated apigenin were assessed and which was further compared with normal, diabetic control and standard drug-treated rat. Histological examination was carried out on 15 days of treatment. Results: Methanolic extract of O. basilicum seed (40 mg/kg) and apigenin (10 mg/kg) produced a significant reduction in fasting blood glucose level (p<0.01) and (p<0.001) respectively in the streptozotocin-induced diabetic rat. Significant differences were observed in oral glucose tolerance test, serum lipid parameters and body weight for methanolic extract of O. basilicum and apigenintreated diabetic rat as compared to diabetic, normal and standard drug-treated rat. The outcome of the histological examinations of the pancreas treated with a methanolic extract of O. basilicum and apigenin showed comparable regeneration of the cells, which were earlier necrosed by streptozotocin. Methanolic extract of O. basilicum and isolated compound apigenin exhibit significant antihyperglycemic and antihyperlipidemic activities in streptozotocin-induced diabetes in the rat. Conclusion: From above findings, it can be concluded that the O. basilicum seed and isolated compound apigenin must be considered as a potential candidate for the treatment of diabetes and lipidlowering activities in streptozotocin-induced diabetes in the rat.

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The influence of mildronate on peripheral neuropathy and some characteristics of glucose and lipid metabolism in rat streptozotocin-induced diabetes mellitus model

Biomeditsinskaya Khimiya ◽

10.18097/pbmc20115705490 ◽

2011 ◽

Vol 57 (5) ◽

pp. 490-500 ◽

Cited By ~ 4

Author(s):

J. Sokolovska ◽

J. Rumaks ◽

N. Karajeva ◽

D. Grinvalde ◽

J. Sharipova ◽

...

Keyword(s):

Diabetes Mellitus ◽

Neuropathic Pain ◽

Blood Glucose ◽

Glucose Tolerance ◽

Tolerance Test ◽

Diabetic Rat ◽

Oral Glucose ◽

Glucose And Lipid Metabolism ◽

Glucose Ingestion ◽

Streptozotocin Induced Diabetes

Streptozotocin (STZ) was used to induce the diabetic rat model. STZ rats were treated with mildronate (100 mg/kg daily, per os or intraperitoneally for 6 weeks). Body weight, blood glucose, triglyceride, ketone body concentrations, glycated hemoglobin percent (HbA1c%), glucose tolerance, and the development of neuropathic pain were monitored throughout the experiment. In the STZ + mildronate group, mildronate treatment caused a significant decrease in mean blood glucose (on week 4) and triglyceride concentrations (on weeks 3-6), significantly slowed the increase in HbA1c% (on week 6) and improved glucose tolerance 120 minutes after glucose ingestion during oral glucose tolerance test versus the STZ group. Mildronate completely protected development of STZ-induced neuropathic pain from the first administration week up to end of the experiment. The obtained data indicate clinical usefulness of the drug for the treatment of diabetes mellitus and its complications.

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Methanolic Extract of Myrsine africana Leaf Ameliorates Hyperglycemia and Dyslipidemia in Alloxan-Induced Diabetic Albino Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/3987656 ◽

2021 ◽

Vol 2021 ◽

pp. 1-14

Author(s):

Yosef Eshetie Amare

Keyword(s):

Glycated Hemoglobin ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Tolerance Test ◽

Oral Glucose ◽

Diabetic Mice ◽

Metabolizing Enzymes ◽

Standard Drug ◽

Significant Difference ◽

Administration Method

Background. Diabetes mellitus is one of the leading public health problems globally, and its prevalence is increasing in Ethiopia. The current drugs for people with diabetes are costly, less effective, and less safe with a challenging administration method. Thus, globally, the need for alternative herbal antidiabetic medicines is increasing. In the previous studies, antioxidant activities have been seen in crude extracts of M. africana leaves, which is an auspicious sign of antidiabetic property. Accordingly, this study has evaluated the antidiabetic and antidyslipidemic activities of methanolic extract of M. africana leaves. Methods. Hypoglycemic and antihyperglycemic activities of the three doses (250 mg/kg, 500 mg/kg, and 1000 mg/kg) of crude methanolic extract of M. africana leaf were studied on normoglycemic, oral glucose-loaded, and alloxan-induced diabetic mice models. The effect of the extract on diabetic dyslipidemia, insulin and glycated hemoglobin levels, carbohydrate-metabolizing enzymes, and body weight was also studied in alloxan-induced diabetic mice. Glibenclamide (5 mg/kg) was used as a standard drug in all cases. Data analysis was carried out using mixed-design ANOVA. A P value of ≤0.05 was considered a statistically significant difference. Results. The methanolic extract of M. africana leaf did not show acute toxicity up to the dose of 5000 mg/kg and showed better glucose utilization in the oral glucose tolerance test. After 14 days of treatment, M. africana leaf extract decreased the blood glucose level, glycated hemoglobin, glucose-6-phosphatase, and fructose-1-6-bisphosphatase in diabetic mice. In contrast, it increased hexokinase and insulin levels in diabetic mice. Moreover, weight loss and dyslipidemia profiles have been corrected significantly in diabetic mice. Conclusion. M. africana leaves showed antihyperglycemic and antidyslipidemic effects in alloxan-induced diabetic mice. That suggests M. africana may be a potential treatment option for diabetes in the future. However, further molecular studies are required to analyze the mechanisms.

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Anti-diabetic and Anti-oxidant Effects of Methanolic Flower Head Extract of Tagetes patula in Streptozotocin and Dexamethasone Induced Diabetic Rats

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs/lpr.2021.11.3.p29-35 ◽

2021 ◽

Vol 11 (3) ◽

Author(s):

Ganga Raju M. ◽

Gayathri S ◽

Suvarchala Reddy V N V L ◽

Anusha K ◽

Pavani K

Keyword(s):

Insulin Resistance ◽

Blood Glucose ◽

Glucose Tolerance Test ◽

Methanolic Extract ◽

Tolerance Test ◽

Oral Glucose ◽

Tagetes Patula ◽

Oral Glucose Tolerance ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes is a chronic metabolic disorder characterized by a high blood glucose concentration, i.e., hyperglycemia or caused by insulin deficiency, often combined with insulin resistance. In the Present study, the antidiabetic and antioxidant activity of the methanolic extract of Tagetes patula flower heads was screened out in rodent models. The pharmacological evaluation was carried out using 200 and 400 mg/kg, b.w. The in-vivo antidiabetic activity was performed in streptozotocin, dexamethasone-induced diabetic models and oral glucose tolerance test. Metformin hydrochloride was used as a standard. The parameters like blood glucose levels, insulin levels, homeostasis assessment of insulin resistance, insulin sensitivity, and fasting glucose-insulin ratio were estimated in these models. The in-vitro antioxidant activity of methanolic extract of Tagetes patula was performed by using hydroxyl radical scavenging assay and Ascorbic acid was used as a standard. The preliminary phytochemical screening of Tagetes patula revealed the presence of phytoconstituents like alkaloids, flavonoids, saponins, steroids, phenols, carbohydrates, triterpenoids, and tannins. The extract showed a significant decrease in the elevated blood glucose levels in streptozotocin, dexamethasone-induced diabetic models, and also in the oral glucose tolerance test. The extract prevented insulin resistance and significantly increased insulin sensitivity in the dexamethasone-induced model. The various phytochemical constituents like alkaloids, flavonoids, saponins, steroids, and phenols might be responsible for lowering the blood glucose levels in the animal models mentioned above. The extract showed significant scavenging activity against hydroxyl free radicals. From the above outcomes, it is flawless that the methanolic extract of Tagetes patula has antidiabetic and antioxidant activities.

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Comparative Evaluation of the Antidiabetic Effects of Different Parts ofCassia fistulaLinn, a Southeast Asian Plant

Journal of Chemistry ◽

10.1155/2013/714063 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

John Wilking Einstein ◽

Mustafa Mohd Rais ◽

Mustafa Ali Mohd

Keyword(s):

Glucose Uptake ◽

Methanolic Extract ◽

Glycosylated Hemoglobin ◽

Tolerance Test ◽

Serum Marker ◽

Diabetic Rats ◽

Oral Glucose ◽

Sprague Dawley ◽

Methanolic Extracts ◽

Isolated Rat

The hypoglycemic effect of the methanolic and aqueous extracts of whole parts ofCassia fistulain both normoglycemic and streptozotocin-nictotinamide induced Type 2 diabetic rats were investigated. Acute toxicity, oral glucose tolerance test and glucose uptake in isolated rat hemidiaphragm were performed in normal rats. Diabetes was induced in Sprague Dawley rats by the administration of streptozotocin-nictotinamide (50, 110 mg/kg b.w., resp.) intraperitoneally. Different extracts ofCassiawas administered to diabetic rats at 250 and 500 mg/kg doses for 21 days. Biochemical parameters like blood glucose, insulin, glycosylated hemoglobin, lipid profile, and serum marker enzymes were determined. The methanolic extract of the bark and leaves were show more effective in causing hypoglycemia in normoglycemic rats. Diabetic rats showed increased levels of glycosylated hemoglobin, reduced levels of plasma insulin, were significantly reverted to near normal after oral administration of the bark and leaf methanolic extracts. Glucose uptake studies in isolated rat hemidiaphragm have shown enhanced peripheral utilization of glucose. Chronic treatment ofCassiaremarkably restored the normal status of the histopathological changes observed in the selected tissues. Dose dependent anti-diabetic effects with the cohorts receiving the methanolic extract of bark followed by leaves ofCassiawas revealed.

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