scholarly journals Anti-inflammatory potential of root and stem extracts of Sesbania sesban

2017 ◽  
Vol 6 (11) ◽  
pp. 1790
Author(s):  
Baljinder Singh ◽  
Rajiv Sharma ◽  
Rajiv Sharma ◽  
Kalia A.N. ◽  
Kalia A.N.
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Diclofenac Sodium ◽  
Disease Process ◽  
Sesbania Sesban ◽  
Paw Edema ◽  
Red Blood Cell Membrane ◽  
Anti Inflammatory

Successive extracts of root and stem of Sesbania sesban were investigated for in-vitro and in-vivo anti-inflammatory potential employing human red blood cell membrane stabilization and rats paw edema methods respectively. Currently much interest is being paid in the search of medicinal plants with potent anti-inflammatory activity which may lead to the discovery of new therapeutic entity. The plant based agents are not only used to suppress the inflammation but also used in different disease conditions where the inflammation responses are amplifying the disease process. The potency of the successive extracts of root and stem of Sesbania sesban were compared with standard diclofenac sodium (10 mg/kg/b.w.). The n-butanol, aqueous and ethyl acetate extracts showed the most significant (p<0.01) whereas chloroform and total alkaloidal extracts showed modereate anti-inflammatory effects on membrane stabilizing action on human red blood cell membrane and inhibition of rats paw edema methods.

scholarly journals ANTI-INFLAMMATORY STUDY OF ANREDERA CORDIFOLIA LEAVES AND CENTELLA ASIATICA HERBS AND ITS COMBINATIONS USING HUMAN RED BLOOD CELL-MEMBRANE STABILIZATION METHOD

2016 ◽  
Vol 9 (5) ◽  
pp. 78 ◽  
Author(s):  
Entris Sutrisno ◽  
Ketut Adnyana I ◽  
Elin Yulinah Sukandar ◽  
Irda Fidrianny ◽  
Widhya Aligita
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Ethyl Alcohol ◽  
Red Blood Cell ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Alcohol Extract ◽  
Red Blood Cell Membrane ◽  
Anti Inflammatory

ABSTRACTObjective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was thehighest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system.This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica).Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human redblood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin andasiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug.Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit thehemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combinationof ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition ofhemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm.Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or ascombinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.

scholarly journals UJI AKTIVITAS ANTIINFLAMASI INFUSA BUNGA PUKUL EMPAT SECARA IN VITRO

2021 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
Hidayatul Ihsan ◽  
Iman Surya Pratama ◽  
Nisa Isnaeni Hanifa
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Test Group ◽  
Inflammatory Activity ◽  
Test Results ◽  
Membrane Stabilization ◽  
Red Blood Cell Membrane ◽  
Mirabilis Jalapa ◽  
Anti Inflammatory

The stew of Mirabilis jalapa L. flower has been used traditionally as an anti-inflammatory. Phytochemical screening of Mirabilis jalapa L. flower showed flavonoid content which is known to have anti-inflammatory activity. This study aims to determine the anti-inflammatory activity of Mirabilis jalapa L. flower infusion using the red blood cell membrane stabilization method by inducing hypotonicity and heat. The test group consisted of Mirabilis jalapa L. flower infusion with a concentration of 2.5%; 5%; and 10% w / v, positive control (diclofenac sodium 0.01% w / v and aspirin 0.01% w / v), and negative control (distilled water). The anti-inflammatory activity of Mirabilis jalapa L. flower infusion was determined by the percentage of membrane stabilization and inhibition of red blood cell hemolysis. Data from the test results were statistically analyzed both parametric and nonparametric with SPSS. Mirabilis jalapa L. flower infusion contains flavonoids, tannins and terpenoids. The test results showed that the 10% concentration of Mirabilis jalapa L. flower infusion had the largest percentage of red blood cell hemolysis protection on hypotonicity induction, that is 99.50% (p>0.05) and the largest percentage of hemolysis inhibition on heat induction, that is 50.27% ( p≤0.05). From these results, it can be concluded that the Mirabilis jalapa L. flower infusion has potential as an anti-inflammatory by stabilizing the red blood cell membrane.

scholarly journals In Vitro Measurement of the Elastic Properties of the Native Red Blood Cell Membrane

2015 ◽  
Vol 11 (3) ◽  
pp. 39 ◽  
Author(s):  
V. A. Sergunova ◽  
E. K. Kozlova ◽  
E. A. Myagkova ◽  
A. M. Chernysh
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Elastic Properties ◽  
Red Blood Cell ◽  
Red Blood Cell Membrane

Uji Aktivitas Anti Inflamasi Ekstrak Bawang Dayak (Eleutherine Americana L. Merr) terhadap Stabilisasi Membran Sel Darah Merah

2019 ◽  
Vol 2 (1) ◽  
pp. 30
Author(s):  
Roji Septian Hardi ◽  
Slamet Slamet ◽  
Laila Kamilla
Keyword(s):  
Diabetes Mellitus ◽  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Diclofenac Sodium ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Purposive Sampling ◽  
Anti Inflammatory ◽  
Eleutherine Americana

Abstract:  Dayak onion plants (Eleutherine americana L. Merr) is one of the most common herbaceous plants used by the community as atraditional medicinal plants. Benefts of dayak onion as a medicine for various diseases such as breast cancer, diabetes mellitus, lowering hypertension, anti-inflammatory and lowering cholesterol.. Based on the research, Dayak bulb extract contains compounds such as Flavonoid, Phenol and Tanin are known to have anti-inflammatory activity. This study aims to determine the difference of anti-inflammatory activity of Dayak extract (Eleutherine americana L. Merr) compared with diclofenac sodium to stabilization of red blood cell membrane. This research is quasi experimental using stabilization method of red blood cell membrane. This study used purposive sampling technique with Dayak extract on concentration samples 0.005, 0.01, 0.02, 0.04 and 0.08% with the repetition of 5 times. Red blood cell lysis Inhibition induced by hipotonis solutionis used as an anti-inflammatory activity measurements. Anti-inflammatory activity of the extract is then compared to positive control (diclofenac sodium). The result of anti-inflammatory activity test showed that the dayak extract which has the highest anti-inflammatory activity was on the extract of 0.08% concentration which was 72.74%, while the most effective concentration of Dayak extract was at a Concentration of 0,02% that is equal to 59,58% of Tukey’s statistical results showed that 0.02% concentration did not differ signifcantly or identical with positive control (diclofenac sodium) at 0.01% concentration of 60.39% with a sample signifcance value of 0.757 ≥ α 0.05 which means that the onion dayak has potential as an antiinflammatoryAbstrak: Tanaman bawang dayak (Eleutherine americana L. Merr) merupakan salah satu jenis tanaman herbal semusim yang lazim digunakan oleh masyarakat sebagai tanaman obat tradisional. Manfaat tanaman bawang dayak sebagai obat berbagai jenis penyakit seperti kanker payudara, diabetes mellitus, menurunkan hipertensi, antiinflamasi dan menurunkan kadar kolesterol. Berdasarkan penelitian, ekstrak umbi bawang dayak mengandung senyawa berupa Flavonoid, Fenol dan Tanin yang diketahui memiliki aktivitas antiinflamasi. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas antiinflamasi ekstrak bawang dayak (Eleutherine americana L. Merr) yang dibandingkan dengan natrium diklofenak terhadap stabilisasi membran sel darah merah. Penelitian ini merupakan penelitian eksperimental semu dengan menggunakan metode stabilisasi membran sel darah merah. Penelitian ini menggunakan teknik purposive sampling dengan sampel ekstrak bawang dayak konsentrasi 0,005, 0,01, 0,02, 0,04 dan 0,08% dengan dilakukan pengulangan sebanyak 5. Penghambatan lisis sel darah merah akibat induksi larutan hipotonis digunakan sebagai ukuran aktivitas antiinflamasi. Aktivitas antiinflamasi dari ekstrak tersebut kemudian dibandingkan dengan kontrol positif (natriun diklofenak). Hasil uji aktivitas antiinflamasi menunjukkan bahwa ekstrak bawang dayak yang memiliki aktivitas antiinflamasi paling tinggi adalah pada ekstrak konsentrasi 0,08% yaitu sebesar 72,74%, sedangkan konsentrasi yang paling efektif dari ekstrak bawang dayak adalah pada konsentrasi 0,02% yaitu sebesar 59,58% dari hasil uji statistik Tukey menunjukkan pada konsentrasi 0,02% tidak berbeda secara bermakna atau identik dengan kontrol positif (natrium diklofenak) pada konsentrasi 0,01% yaitu sebesar 60,39% dengan nilai signifkansi sampel 0,757 ≥ 0,05 ini menunjukkan bahwa bawang dayak memiliki potensi sebagai antiinflamasi.

scholarly journals Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 99
Author(s):  
Xinyi Wu ◽  
Yichen Li ◽  
Faisal Raza ◽  
Xuerui Wang ◽  
Shulei Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Membrane ◽  
Blood Cell ◽  
Sustained Release ◽  
Delivery System ◽  
Red Blood Cell ◽  
Multiple Drug Resistance ◽  
Plga Nanoparticles ◽  
Multiple Drug ◽  
Red Blood Cell Membrane

Multiple drug resistance (MDR) in bacterial infections is developed with the abuse of antibiotics, posing a severe threat to global health. Tedizolid phosphate (TR-701) is an efficient prodrug of tedizolid (TR-700) against gram-positive bacteria, including methicillin-sensitive staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA). Herein, a novel drug delivery system: Red blood cell membrane (RBCM) coated TR-701-loaded polylactic acid-glycolic acid copolymer (PLGA) nanoparticles (RBCM-PLGA-TR-701NPs, RPTR-701Ns) was proposed. The RPTR-701Ns possessed a double-layer core-shell structure with 192.50 ± 5.85 nm in size, an average encapsulation efficiency of 36.63% and a 48 h-sustained release in vitro. Superior bio-compatibility was confirmed with red blood cells (RBCs) and HEK 293 cells. Due to the RBCM coating, RPTR-701Ns on one hand significantly reduced phagocytosis by RAW 264.7 cells as compared to PTR-701Ns, showing an immune escape effect. On the other hand, RPTR-701Ns had an advanced exotoxins neutralization ability, which helped reduce the damage of MRSA exotoxins to RBCs by 17.13%. Furthermore, excellent in vivo bacteria elimination and promoted wound healing were observed of RPTR-701Ns with a MRSA-infected mice model without causing toxicity. In summary, the novel delivery system provides a synergistic antibacterial treatment of both sustained release and bacterial toxins absorption, facilitating the incorporation of TR-701 into modern nanotechnology.

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