scholarly journals Betaine Aldehyde Dehydrogenase (BADH) gene and free amino acid analysis in Rhizophora mucronata Lam. from Thalassery region of Kerala, India.

2018 ◽  
Vol 7 (10) ◽  
pp. 2430
Author(s):  
Anusha Sreeshan ◽  
Meera S.P. ◽  
Anu Augustine
Keyword(s):  
Amino Acids ◽  
Salt Tolerance ◽  
Aldehyde Dehydrogenase ◽  
Free Amino Acids ◽  
Free Amino ◽  
Betaine Aldehyde Dehydrogenase ◽  
Chromatographic Technique ◽  
Rhizophora Mucronata ◽  
Betaine Aldehyde ◽  
Badh Gene

Mangroves are salt tolerant plants inhabiting saline environment. Multiple factors contribute to their salt tolerance and we need multifaceted approach to reveal the mechanisms of salt tolerance in the plant. In the present study, leaves of the mangrove, Rhizophora mucronata grown in the presence and absence of salt were used, free amino acids and the expression of selected genes were analyzed. Chromatographic technique showed the accumulation of free amino acids like proline, glycine, aspartic acid, valine, leucine and glutamic acid in the presence of salt. RNA was isolated from the leaf sample and cDNA was synthesized. Gene specific primers were designed and standardized. Among the genes studied (P5CS, BADH, NHX1), Betaine Aldehyde dehydrogenase (BADH) gene was found to be expressed.

Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase

Euphytica ◽  
2010 ◽  
Vol 178 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Zi-Hui Liu ◽  
Hong-Mei Zhang ◽  
Guo-Liang Li ◽  
Xiu-Lin Guo ◽  
Shou-Yi Chen ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Aldehyde Dehydrogenase ◽  
Betaine Aldehyde Dehydrogenase ◽  
Gene Encoding ◽  
Betaine Aldehyde

scholarly journals Salt tolerance in two gramineae sp.Panicum maximumandEleusine coracana: Glycinebetaine accumulation and expression of betaine aldehyde dehydrogenase mRNA

1997 ◽  
Vol 43 (sup1) ◽  
pp. 999-1002 ◽  
Author(s):  
Hirofumi Saneoka ◽  
Satoshi Ishiguro ◽  
Chie Nagasaka ◽  
Takuo Okada ◽  
Kaori Yamauchi ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Aldehyde Dehydrogenase ◽  
Betaine Aldehyde Dehydrogenase ◽  
Betaine Aldehyde

ION EXCHANGE CHROMATOGRAPHY OF THE FREE AMINO ACIDS IN THE PLASMA OF INFANTS UNDER 2,500 GM AT BIRTH

PEDIATRICS ◽  
1970 ◽  
Vol 45 (4) ◽  
pp. 606-613
Author(s):  
Johanne C. Dickinson ◽  
Herman Rosenblum ◽  
Paul B. Hamilton
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Sensitivity ◽  
Exchange Chromatography ◽  
Chromatographic Technique ◽  
The Individual ◽  
Amino Acid Concentrations

The free amino acids in the plasma of 46 infants who were under 2,500 gm at birth were determined by an ion exchange chromatographic technique of high sensitivity and resolution. Ninety-two plasma samples were collected from the 46 infants on different days after birth, and the data for 23 amino acids plus taurine and ethanolamine were summarized and compared with newborn, full-term and adult levels. In 16 cases tyrosine levels were high; these values are listed separately. With respect to the remaining amino acids, many showed marked changes during the first few postnatal days; but, by the end of the first week, stable patterns had developed. The decrease or increase of the individual amino acid concentrations in these infants compared to infants with birth weights over 2,500 gm and to the adult was not great and seemed to be characteristic for each amino acid. Attention was drawn to the technical details of preparing and analyzing physiological fluids which would minimize the changes in amino acid concentrations resulting from improper handling.

Ion-Exchange Chromatography of Free Amino Acids in Human Intraocular Fluids

1971 ◽  
Vol 17 (4) ◽  
pp. 285-289 ◽  
Author(s):  
Davis G Durham ◽  
Johanne C Dickinson ◽  
Paul B Hamilton
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Subretinal Fluid ◽  
Aqueous Fluid ◽  
Exchange Chromatography ◽  
Chromatographic Technique ◽  
Order Of Magnitude

Abstract Free amino acids were determined in human aqueous, vitreous, and subretinal fluid (50 to 100 µl) from individual eyes by using an ion-exchange chromatographic technique of increased sensitivity. The concentrations of amino acids in aqueous fluid from intraocular malignant melanomas were compared with those found for normal eyes, and some differences were observed. Other pathological conditions investigated were mongolism, intraocular hemangioma, uveitis, Sturge—Weber’s syndrome, and Marfan’s syndrome; only the latter two appeared to have a normal amino acid pattern. Vitreous and subretinal fluid each had a distinctive pattern, with the amino acid concentrations generally lower than in aqueous fluid, except for glutamine, which appeared to be of the same order of magnitude in all intraocular fluids and plasma. Postmortem and eye-bank aqueous fluids were also analyzed, and showed considerable variability from normal. The presence of an additional 28 unknown compounds was demonstrated in aqueous fluid.

Sign in / Sign up

Export Citation Format

Share Document