Ion-Exchange Chromatography of Free Amino Acids in Human Intraocular Fluids

1971 ◽  
Vol 17 (4) ◽  
pp. 285-289 ◽  
Author(s):  
Davis G Durham ◽  
Johanne C Dickinson ◽  
Paul B Hamilton
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Subretinal Fluid ◽  
Aqueous Fluid ◽  
Exchange Chromatography ◽  
Chromatographic Technique ◽  
Order Of Magnitude

Abstract Free amino acids were determined in human aqueous, vitreous, and subretinal fluid (50 to 100 µl) from individual eyes by using an ion-exchange chromatographic technique of increased sensitivity. The concentrations of amino acids in aqueous fluid from intraocular malignant melanomas were compared with those found for normal eyes, and some differences were observed. Other pathological conditions investigated were mongolism, intraocular hemangioma, uveitis, Sturge—Weber’s syndrome, and Marfan’s syndrome; only the latter two appeared to have a normal amino acid pattern. Vitreous and subretinal fluid each had a distinctive pattern, with the amino acid concentrations generally lower than in aqueous fluid, except for glutamine, which appeared to be of the same order of magnitude in all intraocular fluids and plasma. Postmortem and eye-bank aqueous fluids were also analyzed, and showed considerable variability from normal. The presence of an additional 28 unknown compounds was demonstrated in aqueous fluid.

ION EXCHANGE CHROMATOGRAPHY OF THE FREE AMINO ACIDS IN THE PLASMA OF INFANTS UNDER 2,500 GM AT BIRTH

PEDIATRICS ◽  
1970 ◽  
Vol 45 (4) ◽  
pp. 606-613
Author(s):  
Johanne C. Dickinson ◽  
Herman Rosenblum ◽  
Paul B. Hamilton
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Sensitivity ◽  
Exchange Chromatography ◽  
Chromatographic Technique ◽  
The Individual ◽  
Amino Acid Concentrations

The free amino acids in the plasma of 46 infants who were under 2,500 gm at birth were determined by an ion exchange chromatographic technique of high sensitivity and resolution. Ninety-two plasma samples were collected from the 46 infants on different days after birth, and the data for 23 amino acids plus taurine and ethanolamine were summarized and compared with newborn, full-term and adult levels. In 16 cases tyrosine levels were high; these values are listed separately. With respect to the remaining amino acids, many showed marked changes during the first few postnatal days; but, by the end of the first week, stable patterns had developed. The decrease or increase of the individual amino acid concentrations in these infants compared to infants with birth weights over 2,500 gm and to the adult was not great and seemed to be characteristic for each amino acid. Attention was drawn to the technical details of preparing and analyzing physiological fluids which would minimize the changes in amino acid concentrations resulting from improper handling.

AMINO ACID COMPOSITION OF UREDOSPORES OF PUCCINIA GRAMINIS TRITICI

1965 ◽  
Vol 43 (6) ◽  
pp. 695-699 ◽  
Author(s):  
David Stefanye ◽  
Kenneth R. Bromfield
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Spore Wall ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Puccinia Graminis ◽  
Total Free Amino Acids

Uredospores of Puccinia graminis var. tritici (race 56) were analyzed quantitatively for total free amino acids and ninhydrin-positive substances by ion-exchange chromatography. Extracts of these substances were obtained by leaching the spores and by re-extracting leached spores with boiling water. Thirty-five ninhydrin-positive compounds were found and identified. The leach extract differed quantitatively from the extract obtained by boiling although both contained the same 35 substances. It is proposed that there are easily extractable ninhydrin-positive substances coating the spore wall and ninhydrin-positive substances in the protoplasm that can be extracted only with difficulty.

scholarly journals Free amino acids in fruit bodies of some wood destroying fungi

2014 ◽  
Vol 14 (1-2) ◽  
pp. 151-155
Author(s):  
Wiesław Tadeusiak ◽  
Eliza Balicka
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Polyporus Squamosus

Concentration of free amino acids in the following bracket fungi: <i>Climacodon septentrionalis</i> (Fr) P. Karst, <i>Hapalopilus croceus</i> (Pers. ex Fr.) Donk., <i>Laetiporusus sulphurens</i> (Bull. ex Fr.) Murill and <i>Polyporus squamosus</i> Huds ex Fr., were determined by ion-exchange chromatography.

Chromatographic Separation of Free Amino Acids in Table Sirups

1971 ◽  
Vol 54 (1) ◽  
pp. 61-65
Author(s):  
Arthur Russell Johnson ◽  
Richard L Corliss ◽  
Enrique Fernandez-Flores
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Butyl Esters ◽  
Tlc Analysis

Abstract Qualitative chromatographic methods for the separation of free amino acids in table sirups are presented to aid in the development of chemical indices of composition which may be useful in establishing the identity of sirups and detecting their adulteration. Free amino acids in 2 table sirups were isolated on ion exchange columns and eluted with dilute ammonia. The concentrated amino acid mixture in the eluate was spotted directly on silica gel G plates for TLC analysis, or the amino acids were converted to their N-trifluoroacetyl n-butyl esters for GLC analysis. As many as 16 amino acids were qualitatively separated and identified and a potential for quantitative analysis was demonstrated.

Effect of Deproteinating Agents (Picric Acid and Sulfosalicylic Acid) on Analysis for Free Amino Acids in Swine Blood and Tissue

1969 ◽  
Vol 52 (5) ◽  
pp. 981-984 ◽  
Author(s):  
J E Knipfel ◽  
D A Christensen ◽  
B D Owen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino ◽  
Biological Fluids ◽  
Picric Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sulfosalicylic Acid ◽  
Coefficients Of Variation

Abstract Amino acid analyses were performed on samples of blood, liver tissue, loin muscle, and ham muscle by ion exchange chromatography after deproteination of the samples with picric acid or sulfosalicylic acid (SSA). Resolution of threonine and serine from the ion exchange column was poor when SSA was used as the deproteinating agent. Twelve of sixteen amino acids were higher (P &lt; 0.05) in serum deproteinated with picric acid as compared to concentrations determined after SSA deproteination. Amino acid values for ham muscle tended to be higher after deproteination with picric acid; however, with liver and loin muscle samples, the values were somewhat higher after SSA deproteination. In both serum and tissue analyses, coefficients of variation were lower for niGSt amino acids when picric acid was utilized as the deproteinating agent. The latter observation, in particular, suggests that picric acid is preferable to SSA as a deproteinating agent before amino acid analyses of biological fluids. Standardization of methods of deproteination is needed to allow meaningful comparisons of data.

scholarly journals Determination of free amino acid and biogenic amine contents of hungarian sparkling wines

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S287-S289 ◽  
Author(s):  
L. Simon-Sarkadi ◽  
E. Szőke ◽  
A. Kerekes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biogenic Amines ◽  
Free Amino Acid ◽  
Biogenic Amine ◽  
Free Amino Acids ◽  
Free Amino ◽  
Exchange Chromatography ◽  
Sparkling Wine

Comparative study was conducted on the basis of free amino acids and biogenic amines of Hungarian sparkling wines originated from 3 producers (Törley, Hungária, Balaton Boglár). Determination of amino acids and biogenic amines was accomplished by ion-exchange chromatography using an amino acid analyser. The dominant free amino acids in sparkling wines were proline and arginine and the major biogenic amine was spermidine. Based on results of chemometric analyses, free amino acid and biogenic amine contents seemed to be closely related to quality and the technology of sparkling wine making.

ENDOGENOUS RENAL CLEARANCE RATES OF FREE AMINO ACIDS IN PRE-PUBERTAL CHILDREN (Employing an Accelerated Procedure for Elution Chromatography of Basic Amino Acids on Ion Exchange Resin)

PEDIATRICS ◽  
1965 ◽  
Vol 36 (4) ◽  
pp. 592-598 ◽  
Author(s):  
Charles R. Scriver ◽  
Eluned Davies
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Renal Clearance ◽  
Individual Variation ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Concentrations ◽  
Exchange Chromatography ◽  
Clearance Rates ◽  
Basic Amino Acids

Six male and three female children in good health and between the ages of 3 and 10 years were studied. Fasting plasma concentrations and urine excretion rates of free amino acids were measured using automated ion exchange chromatography, utilizing a modified method for analysis of the basic amino acids. Endogenous clearance rates and net tubular absorption of plasma amino acids were also calculated. The plasma concentration of free amino acids is generally slightly lower than in the adult subjects and renal clearance rates are slightly higher. Less than 5% of the filtered amino acid load is excreted in the urine. Intra-individual variation tends to be smaller than inter-individual variation.

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