Extraction of Total Proteins from Rice Plant

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (21) ◽  
Author(s):  
Da-Gin Lin ◽  
Chang-Sheng Wang
Keyword(s):  
Author(s):  
Y. R. Chen ◽  
Y. F. Huang ◽  
W. S. Chen

Acid phosphatases are widely distributed in different tisssues of various plants. Studies on subcellular localization of acid phosphatases show they might be present in cell wall, plasma lemma, mitochondria, plastid, vacuole and nucleus. However, their localization in rice cell varies with developmental stages of cells and plant tissues. In present study, acid phosphatases occurring in root cap are examined.Sliced root tips of ten-day-old rice(Oryza sativa) seedlings were fixed in 0.1M cacodylate buffer containing 2.5% glutaraldehyde for 2h, washed overnight in same buffer solution, incubated in Gomori's solution at 37° C for 90min, post-fixed in OsO4, dehydrated in ethanol series and finally embeded in Spurr's resin. Sections were doubly stained with uranyl acetate and lead citrate, and observed under Hitachi H-600 at 75 KV.


2020 ◽  
Vol 13 (3) ◽  
pp. 237-253
Author(s):  
Rokia Sahnoune ◽  
Malika Bouchenak

BACKGROUND: Appropriate nutrition is important to maintain health, growth, and development of adolescent athlete performance. OBJECTIVE: To evaluate the impact of nutritional intervention (NI) promoting Mediterranean diet (MD), on food consumption, and adherence to MD, in scholar adolescent athletes. METHODS: Eighty athletes (Females/Males, 39/41) aged 15±1 years participated to NI. MD Adherence was assessed, using Kidmed index, at baseline, and at 6 months. Food intake was evaluated by 24-hour recall at baseline, and at four NI times. RESULTS: At baseline, 61% of athletes had poor MD adherence, and after NI, this value decreased to 10%. At 6 months of NI, 73% of males vs 46% of females had good MD adherence (≥8). Energy intake represented 2369 Kcal/d, at baseline, and was less than recommended value. After NI, significant increase was observed in energy intake (p = 0.001). Mean fiber intake (19 and 21 g/d in females and males, respectively) was less than adequate intake (26/38 g/d), but increased after NI (p = 0.000). Enhancement was noted in total, and complex carbohydrates, and total proteins, and values were increased with time (p = 0.000, p = 0.000, p = 0.003). CONCLUSION: Nutritional intervention, based on Mediterranean diet, improves total energy, and macronutrients intake, and increases MD adherence scores in adolescents athletes.


1930 ◽  
Vol 2 (2) ◽  
pp. 90-95
Author(s):  
MANTARO KONDO ◽  
TAMOTSU OKAMURA
Keyword(s):  

1956 ◽  
Vol 24 (3) ◽  
pp. 151-153
Author(s):  
Noboru YAMADA ◽  
Yasuo OTA

1983 ◽  
Vol 52 (4) ◽  
pp. 468-474
Author(s):  
Masahiko ICHII ◽  
Yoshibumi IWAMOTO
Keyword(s):  

Author(s):  
Shanmugapriya K. ◽  
T. Murugan ◽  
Thayumanavan Tha

Medicinal plants plays a significant role in the pharmaceutical industry. In present scenario, the need is to explore, identify and utilize this new medicinal plant on one hand and, on the other, to help conserve the existing but threatened species of rare medicinal plant.This present research work was carry out for the first time in South India to analyse and estimate the biochemical profiles of various extracts of fresh parts of Gnaphalium polycaulon pers., plant. The biochemical composition such as total carbohydrates, total proteins, total lipids, total phenols, cholesterol, total chlorophylls and reducing sugar, sterols were estimated using the standard procedure in fresh plant material.The biochemical analysis of Gnaphalium polycaulon plant showed the presence of various phytochemicals. The results of the present study supplement the usage of the studied plant which possesses several bioactive compounds and used as food and also as medicine.The results of present studies demonstrated that Gnaphalium polycaulon plant could be a sourceof valuable information and a guideline for the scientists, researchers in India and also all over the world


2018 ◽  
Vol 69 (5) ◽  
pp. 1125-1128
Author(s):  
Daniela G. Balan ◽  
Dan Piperea Sianu ◽  
Iulia I. Stanescu ◽  
Dorin Ionescu ◽  
Andra Elena Stroescu Balcangiu ◽  
...  

Assessment of changes in total proteins level, serum and saliva IgG and IgA levels, serum IgM level, serum and saliva IgA/IgG ratio. The study was conducted on a group of 40 subjects, divided into 2 lots: the first lot consisting of 20 healthy individuals and the second consisting of 20 patients with hepatitis with hepatitis A virus (HAV). The levels of total proteins, serum and saliva IgG and IgA, serum IgM and serum and saliva IgA/IgG ratio have higher values in patients with hepatitis A, in comparison to healthy subjects, without necessarily exceeding the maximum admitted value. The results are significant from a statistical point of view. Due to the sensitivity and specificity of salivary anti-HAV IgM and IgG in patients with acute hepatitis A, compared with healthy subjects, there is a possibility of using salivary immunological tests instead of serum tests for the diagnosis and epidemiological study of HAV infection.


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