scholarly journals Evaluación citológica y microbiológica de lavados uterinos en bovinos con problemas reproductivos (estudio preliminar)

2011 ◽  
pp. 2711-2720
Author(s):  
Milena Sánchez L ◽  
Carolina González C ◽  
Rubiela Castañeda S ◽  
Adriana Pulido V ◽  
Humberto Guáqueta M ◽  
...  
Keyword(s):  
Arcanobacterium Pyogenes ◽  
Actinomyces Pyogenes

RESUMENObjetivo. Determinar las características citológicas y microbiológicas de lavados uterinosde bajo volúmen en hembras con problemas reproductivos en dos hatos del AltiplanoCundiboyacense. Materiales y métodos. Se evaluaron 21 muestras de lavados uterinosde vacas con historia de problemas reproductivos procedentes de dos hatos lecheros, enel primer hato ubicado en Sibaté (Cundinamarca) se muestrearon 10 animales y en elsegundo ubicado en Ventaquemada (Boyacá) se obtuvieron las 11 muestras restantes. Serealizó evaluación macroscópica y microscópica de cada uno de los lavados uterinos; parala evaluación citológica se utilizaron las tinciones de Gram y Wright. La determinación de laflora bacteriana se realizó mediante técnicas estándar en microbiología para la identificaciónde bacterias no exigentes, adicionalmente se realizaron pruebas de sensibilidad por latécnica de difusión de Kirby-bauer. Resultados. En la citología se observaron bacilosy cocos gram positivos, células epiteliales de descamación uterina (CEDU) y respuestainflamatoria (RI) mediada por polimorfonucleares (PMN). Los aislamientos bacterianoscorrespondieron a Lactobacillus sp como flora bacteriana normal del tracto genital (16.66%)y Klebsiella sp como flora acompañante (16.66%); adicionalmente se aislaron bacteriaspatógenas causantes de problemas reproductivos como Streptococcus sp. β hemolítico(33.33%), Streptococcus sp α hemolítico (50%) y Streptococcus sp. γ hemolítico (50%)y Arcanobacterium pyogenes (Corynebacterium-Actinomyces pyogenes) (16.66%); en un9.52% de las muestras se observaron espiroquetas. Conclusiones. Se observó una relacióndirecta entre el aspecto macroscópico de los lavados uterinos, la respuesta citológica y elaislamiento de bacterias.

Lähmungserscheinungen bei Mastschweinen aufgrund einer bakteriellen Hirnstamm-Meningoenzephalitis, verursacht durch eine aufsteigende Zahnfach- und Kieferhöhlenentzündung

2007 ◽  
Vol 35 (05) ◽  
pp. 377-379
Author(s):  
A. Zankl ◽  
K. Matiasek ◽  
W. Schmahl ◽  
K. Heinritzi ◽  
M. Ritzmann ◽  
...  
Keyword(s):  
Klinische Relevanz ◽  
Neurologische Untersuchung ◽  
Allen Vier ◽  
Leptospira Spp ◽  
Arcanobacterium Pyogenes

Zusammenfassung Gegenstand und Ziel: Diagnostische Abklärung von Lähmungserscheinungen, die vereinzelt bei Mastschweinen bis 60 kg in einem Bestand mit 900 Mastplätzen auftraten. Material und Methoden: Ein männlich-kastrierter Mastläufer aus dem Bestand wurde in die Klinik für Schweine der Ludwig-Maximilians-Universität München eingeliefert und klinisch, labordiagnostisch und pathologisch- anatomisch untersucht. Ergebnisse: Der schlecht genährte Läufer befand sich in rechter Seitenlage mit Kopfschiefhaltung nach rechts. Er hatte kein Stehvermögen und Gehversuche waren auch mit Unterstützung nicht möglich. Die neurologische Untersuchung ergab einen normalen Analreflex, normale After- und Zwischenklauenreflexe an allen vier Gliedmaßen sowie eine erhaltene Hautsensibilität. Der Kornealreflex des rechten Auges war erloschen. Die serologische Untersuchung lieferte keine Hinweise auf Aujeszky‘sche Krankheit (AK), Klassische Schweinepest (KSP) und Leptospira spp. Die Werte der hämatologischen und klinisch-chemischen Parameter lagen im Referenzbereich. Pathologisch-anatomisch wurde eine rechtsseitige Hirnstamm-Meningoenzephalitis mit Isolierung von Arcanobacterium pyogenes nachgewiesen. Der neuropathologische Befund ergab als Ursache für die Meningoenzephalitis eine Zahnfach- und Kieferhöhlenentzündung mit Ausbreitung über den Nervus maxillaris. Schlussfolgerung und klinische Relevanz: Als Ursache für Lähmungen der Gliedmaßen bei Läufern sollten auch aufsteigende Zahnfachentzündungen durch Zahnfrakturen oder falsch durchgeführte zootechnische Maßnahmen (Zähnezwicken) in Betracht gezogen werden.

Electroporation-Mediated Transformation ofArcanobacterium (Actinomyces) pyogenes

Plasmid ◽  
1997 ◽  
Vol 38 (2) ◽  
pp. 135-140 ◽  
Author(s):  
B.Helen Jost ◽  
Stephen J. Billington ◽  
J.Glenn Songer
Keyword(s):  
Actinomyces Pyogenes

scholarly journals Ulcerative balanitis and vulvitis of Dorper sheep in South Africa : a study on its aetiology and clinical features

2005 ◽  
Vol 76 (4) ◽  
Author(s):  
A. Kidanemariam ◽  
J. Gouws ◽  
M. Van Vuuren ◽  
B. Gummow
Keyword(s):  
South Africa ◽  
Clinical Features ◽  
Clinical Signs ◽  
Regular Feature ◽  
Clinically Normal ◽  
Large Colony ◽  
Mycoplasma Mycoides ◽  
Dorper Sheep ◽  
Arcanobacterium Pyogenes ◽  
Apparently Healthy

Ovine ulcerative balanitis and vulvitis in sheep of the Dorper breed has been observed in South Africa since 1979. Its aetiology has not been conclusively resolved, and there is some discrepancy in descriptions of its clinical features. In order to identify the pathogenic microorganism / s that contribute to the occurrence of the disease, the microflora in the genital tracts of both clinically healthy and affected sheep were isolated and compared. Bacteriological examination of materials from affected and unaffected sheep resulted in the isolation of Arcanobacterium pyogenes from 44.2 % and 17.2 % of them respectively. This difference is statistically significant (P < 0.01). Seventy-four per cent of the isolates originated from severe clinical cases. Mycoplasmas were isolated from 49.3 % of 116 clinically normal sheep and 78.2%of 104 affected sheep. There were significant differences in their rates of isolation in clinical groups (P < 0.05). Of all the mycoplasma isolates, Mycoplasma mycoides mycoides large colony variant (MmmLC) was isolated from 61.5 % of clinically diseased sheep while 6.0 % of the isolates were from apparently healthy animals (P < 0.05). The study threw light on the prevalence of mycoplasmas in the genital tract of apparently healthy sheep and, at the same time the identity of the mycoplasma pathogen associated with ulcerative balanitis and vulvitis was revealed. The findings of this investigation therefore confirmed the involvement of mycoplasma, particularly that of MmmLC large colony, in the disease in Dorper sheep in South Africa, and it was concluded that this microorganism is an important pathogen of balanitis and vulvitis in them. The study furthermore demonstrated a probable synergism between A. pyogenes and MmmLC. Finding these 2 organisms together occurred 53.4 times more frequently in the affected sheep than in the unaffected, which emphasises the probable multifactorial nature of the disease. The association between age and the presence of clinical signs was statistically significant. It was found that young sheep were more likely to have lesions than adult sheep. Clinical observations showed that the typical ulceration appears to be confined to the glans penis and lips of the vulva; no ulceration was observed on the shaft of the penis and prepuce or vaginal vestibule. In uncomplicated cases inflammation of the prepuce and vaginal vestibule is not a regular feature of the disease. Therefore the names ulcerative balanitis and vulvitis most accurately describe the nature of the disease in South Africa.

Summer mastitis in heifers: Studies on the seasonal occurrence of Actinomyces pyogenes, Peptostreptococcus indolicus and Bacteroidaceae in clinically healthy cattle in Denmark

1992 ◽  
Vol 30 (2-3) ◽  
pp. 243-255 ◽  
Author(s):  
Mogens Madsen ◽  
Gunner Høi Sørensen ◽  
Bent Aalbaek ◽  
Jørgen Willy Hansen ◽  
Henrik Bjørn
Keyword(s):  
Seasonal Occurrence ◽  
Healthy Cattle ◽  
Actinomyces Pyogenes

scholarly journals Molecular identification and further characterization of Arcanobacterium pyogenes isolated from bovine mastitis and from various other origins

2011 ◽  
Vol 94 (4) ◽  
pp. 1813-1819 ◽  
Author(s):  
M. Hijazin ◽  
H. Ülbegi-Mohyla ◽  
J. Alber ◽  
C. Lämmler ◽  
A.A. Hassan ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Bovine Mastitis ◽  
Arcanobacterium Pyogenes

scholarly journals Citologia aspirativa no diagnóstico da linfadenite em ovinos

2011 ◽  
Vol 31 (10) ◽  
pp. 839-843 ◽  
Author(s):  
Márcio G Ribeiro ◽  
Alexandra F Belotta ◽  
Marta C Fernandes ◽  
Rafael Guena ◽  
Geraldo de Nardi Júnior ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Corynebacterium Pseudotuberculosis ◽  
Arcanobacterium Pyogenes

O presente estudo investigou o uso da cito-(73,0%), Arcanobacterium pyogenes (6,0%), Streptococlogia aspirativa com agulha fina no diagnóstico da lin-cus spp. β hemolítico (5,0%) e Escherichia coli (4,0%) fadenite em ovinos e a ocorrência de microrganismos foram os microrganismos mais frequentes nos animais nos linfonodos com lesões, com ênfase no isolamento de com linfadenite. Streptococcus spp. (21,0%) e Staphylo-Corynebacterium pseudotuberculosis. Foram utilizados coccus spp. (7,0%) foram as bactérias isoladas com 100 linfonodos de ovinos com aumento de volume su-maior frequência nos linfonodos sem lesões colhidos em gestivos de linfadenite e 100 linfonodos de ovinos sem abatedouro. A punção aspirativa com agulha fina perlesões, colhidos em abatedouro. C. pseudotuberculosis mitiu identificar microrganismos "corineformes" em 79 (79,0%) animais com linfadenite e, destes, 73 (73,0%) foram identificados como C. pseudotuberculosis. Nenhuma linhagem de C. pseudotuberculosis foi isolada dos linfonodos dos animais sem lesões. Concluiu-se que C. pseudotuberculosis foi o microrganismo mais freqüente nos ovinos com linfadenite, e que a citologia aspirativa pode ser utilizada como método de triagem no diagnóstico da linfadenite caseosa ovina

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