scholarly journals Callogénesis in vitro de durazno (Prunus persica L.) var. Huayco rojo a partir de explantes foliares

2021 ◽  
Vol 23 (1) ◽  
pp. e2032
Author(s):  
Angel David Hernández-Amasifuen ◽  
Anthony Apolinario Cortez-Lázaro ◽  
Alexis Argüelles-Curaca ◽  
Hermila Belba Díaz-Pillasca
Keyword(s):  
Prunus Persica

El durazno (Prunus persica L.) presenta en la actualidad gran importancia económica para el Perú, el incrementar su exportación cada año, siendo la variedad Huayco rojo el de mayor producción y exportación por presentar un agradable sabor y gran aceptación en el mercado exterior. En la búsqueda de expandir las áreas de este cultivo se han realizado estudios de multiplicación empleando métodos biotecnológicos como la micropropagación in vitro, pero se han presentado problemas con esta especie por ser de comportamiento recalcitrante. Por lo que dentro de las alternativas para obtener mayor número de clones de gran calidad y productividad el presente trabajo de investigación tuvo como objetivo desarrollar una metodología eficiente de inducción de callos in vitro en durazno variedad Huayco rojo a partir de hojas. Determinando primero la concentración de hipoclorito de sodio que permita obtener mayor desinfección y menor daño a los explantes de hojas de durazno, Posteriormente los explantes fueron introducidos en medios de cultivo MS adicionado con tratamientos de 2,4-D y agua de coco en diferentes concentraciones. Induciendo callos in vitro en durazno variedad Huayco rojo a partir de hojas, obteniendo 100% de inducción de callos empleando a partir de 1 mg/L de 2,4-D más 50 mL/L de agua de coco. Finalmente, los resultados de esta investigación permiten recomendar el uso de 2,4-D y agua de coco en la inducción del proceso de callogénsis en Prunus persica L. variedad Huayco rojo.

Intercellular Invertase in Developing Peach Mesocarp

1988 ◽  
Vol 15 (3) ◽  
pp. 377 ◽  
Author(s):  
TD Ugalde ◽  
DJ Chalmers ◽  
PH Jerie
Keyword(s):  
Dry Matter ◽  
Prunus Persica ◽  
Total Activity ◽  
Insoluble Fraction ◽  
Acid Invertase ◽  
Dry Matter Accumulation ◽  
Tissue Slices ◽  
Insoluble Particles

Acid invertase (β-fructofuranosidase, EC 3.2.1.26) was extracted from peach mesocarp (Prunus persica (L.) Batsch) using a range of extraction conditions. The enzyme always was attached to insoluble particles in the crude homogenate and was bound by a mechanism that could not have arisen during extraction. The activity in the insoluble fraction made up (essentially) all of the total activity extracted from the tissue and was the same as the activity shown by whole tissue slices placed directly into the assay solution. These results demonstrate that most of the acid invertase in developing peach mesocarp is located outside the cell. The amount of this enzyme, as measured in vitro, did not change during development at times when the rate of dry matter increase was changing rapidly. Either the action of intercellular invertase is not associated with the control of dry matter accumulation in peach mesocarp, or control is effected through activity of the enzyme in vivo, not its synthesis or degradation.

scholarly journals Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 178 ◽  
Author(s):  
Sabbadini ◽  
Ricci ◽  
Limera ◽  
Baldoni ◽  
Capriotti ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Prunus Persica ◽  
Fluorescent Protein ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Prunus Amygdalus ◽  
Indirect Organogenesis ◽  
Egfp Gene ◽  
Rnai Construct

Prunus spp. is one of the most recalcitrant fruit tree species in terms of in vitro regeneration and transformation, mostly when mature tissues are used as explants. The present study describes the in vitro regeneration via indirect organogenesis, and Agrobacterium tumefaciens-mediated transformation of the peach rootstock Hansen 536 (Prunus persica × Prunus amygdalus) through the use of meristematic bulks (MBs) as starting explants. Efficient adventitious shoot regeneration was obtained when Hansen 536 MBs were cultured on an optimized medium consisting of modified McCown Woody Plant medium (WPM) enriched with 4.4 M 6-Benzyladenine (BA), 0.1 M 1-Naphthaleneacetic acid (NAA) and 6.0 g L−1 plant agar S1000 (B&V). MB slices were used later as starting explants for Agrobacterium-mediated transformation to introduce an RNAi construct “ihp35S-PPV194” against PPV virus. Transgenic events were identified by both green fluorescent protein (GFP) screening and kanamycin selection at different concentrations (0, 17 or 42 M). GFP-fluorescent proliferating callus lines were selected and confirmed to stably express the ihp35S-PPV194::eGFP gene construct by molecular analysis. Although shoot regeneration from these transgenic calli has not been obtained yet, this represents one of the few examples of successful attempts in peach genetic transformation from somatic tissues, and also serves as a useful in vitro system for future gene functional analysis in peach.

Plant regeneration from in vitro leaves of the peach rootstock ‘Nemaguard’ (Prunus persica × P. davidiana)

2010 ◽  
Vol 101 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Houcheng Zhou ◽  
Ming Li ◽  
Xia Zhao ◽  
Xiucai Fan ◽  
Aiguang Guo
Keyword(s):  
Plant Regeneration ◽  
Prunus Persica

ADVENTITIOUS SHOOTS FROM COTYLEDONS OF IMMATURE CHERRY AND APRICOT EMBRYOS

1986 ◽  
Vol 66 (4) ◽  
pp. 953-959 ◽  
Author(s):  
W. DAVID LANE ◽  
F. COSSIO
Keyword(s):  
Sweet Cherry ◽  
Prunus Persica ◽  
Immature Embryo ◽  
Adventitious Shoots ◽  
Prunus Armeniaca ◽  
Mature Embryos ◽  
Stage Of Development ◽  
Physiological Stage ◽  
Murashige And Skoog Medium

Immature embryos of Prunus armeniaca (apricot) and Prunus persica (peach) collected 20–30 d from anthesis were cultured on Murashige and Skoog medium supplemented with benzyladenine (BA) and various auxins to study their potential for regeneration. Both species developed adventitious buds on cotyledons when cultured in vitro. Apricot frequency of regeneration was as high as 100% when BA (5.0 μM) and 2,4-D (1.0 μM) were included in the medium. Cherry response was less than apricot (up to 70%) and the maximum frequency of regeneration occurred using media with BA alone (3.0 μM). Auxin was inhibitory to sweet cherry regeneration. The physiological stage of development was very important for regeneration from both species since regeneration did not occur when very young or fully mature embryos were used as explants. Apricot plants were produced by rooting shoots which developed from the regenerated buds on the cotyledons.Key words: Apricot, sweet cherry, regeneration, immature embryo, cotyledon, tissue culture

scholarly journals First Report of the Peach Brown Rot Fungus Monilinia fructicola Resistant to Demethylation Inhibitor Fungicides in New Jersey

Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 126-126 ◽  
Author(s):  
A. Burnett ◽  
N. Lalancette ◽  
K. McFarland
Keyword(s):  
South Carolina ◽  
New Jersey ◽  
Cold Storage ◽  
Prunus Persica ◽  
Crop Protection ◽  
Brown Rot ◽  
Polymorphism Analysis ◽  
Monilinia Fructicola ◽  
Resistant Strains

Reduced sensitivity and resistance of Monilinia fructicola to demethylation inhibitors (DMIs; fungicide group 3) have been previously found in stone fruit orchards in Georgia, South Carolina, Ohio, and New York (2). Resistance development is a major concern because of the importance of DMIs for brown rot management. Eleven single-spore isolates, originally collected during 2006 from separate commercial peach (Prunus persica) orchards in southern New Jersey, were removed from cold storage (5°C) in early 2008 and examined in vitro for resistance to the DMI propiconazole (Orbit 3.6EC; Syngenta Crop Protection, Inc., Greensboro, NC). After 19 months at 5°C, isolate 7 was inhibited 53.4% in growth on potato dextrose agar (PDA) amended at the discretionary dose of 0.3 μg/ml propiconazole; inhibition of the remaining isolates ranged from 81.4 to 100%. Inhibition values were based on two replications of eight colonies per isolate performed after incubation at 25°C for 4 days. Because of the previously reported relationship between duration of cold storage and propiconazole sensitivity, isolate 7 was tentatively deemed resistant (1). To confirm the in vitro results, isolates were grown at 25°C for 7 days on cellophane over PDA. Genomic DNA was isolated from mycelium with the DNeasy Plant Mini Kit (Qiagen, Inc., Valencia, CA). PCR with primers INS65-F and INS65-R was conducted on a GeneAmp thermal cycler (Applied Biosystems, Inc., Foster City, CA) as described previously to amplify a 65-bp region named ‘Mona’ associated with DMI resistance (2). PCR products were separated via electrophoresis on 0.8% agarose gel. The primers amplified a 376-bp fragment from isolate 7 and a 311-bp fragment from all other isolates, thus indicating the presence of Mona in isolate 7. Restriction fragment length polymorphism analysis using the BsrBI enzyme, specific to a single restriction site within Mona, was conducted on the amplified fragments from all isolates. Electrophoresis results showed digestion of the 376-bp fragment from isolate 7 into 140-bp and 236-bp fragments, thereby confirming the presence of Mona; none of the 311-bp fragments from the remaining isolates were cut by BsrBI. Although economic loss from brown rot has not been reported in New Jersey, these results show that propiconazole-resistant strains have been detected since 2006 and it is most likely that resistant strains of the pathogen are still present in commercial peach orchards. To combat this risk, current brown rot control recommendations are incorporating quinone outside inhibitors (QoIs; fungicide group 11) and carboxamides (fungicide group 7) into control programs as a resistance management strategy. More extensive sampling is planned to ascertain the prevalence and location of resistant strains. References: (1) K. D. Cox et al. Phytopathology 97:448, 2007. (2) C.-X. Luo et al. Plant Dis. 92:1099, 2008.

scholarly journals A cRNA Probe Detects Prunus Necrotic Ringspot Virus in Three Peach Cultivars after Micrografting and in Peach Shoots following Long-term Culture at 4 °C

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 346-347 ◽  
Author(s):  
K. Heuss ◽  
Q. Liu ◽  
F.A. Hammerschlag ◽  
R.W. Hammond
Keyword(s):  
Prunus Persica ◽  
Blot Hybridization ◽  
Ringspot Virus ◽  
Shoot Cultures ◽  
Crna Probe ◽  
Reading Frame ◽  
Prunus Necrotic Ringspot Virus ◽  
Repeated Use ◽  
Graft Success

As part of a program to develop transgenic peach (Prunus persica L. Batsch) cultivars with resistance to Prunus necrotic ringspot virus (PNRSV), we are testing a system for measuring virus in peach shoot cultures. Micrografting in vitro is used for inoculation and slot-blot hybridization, with a digoxigenin (DIG)-labeled cRNA probe complementary to the 5′ open reading frame (ORF) of PNRSV RNA 3, for detection. In this study, we investigated whether infected shoots maintain virus infection over long periods of culture at 4 °C and if PNRSV-infected `Suncrest' shoot cultures can serve as graft bases to transmit virus equally well into cultivars Nemaguard, Springcrest, and Suncrest. The results of RNA hybridization analysis showed that virus was present in extracts of leaf samples from 2-year-old PNRSV-infected `Suncrest' shoots that had been subjected to varying lengths of incubation at 4 °C in the dark, suggesting that infected shoots can be maintained for repeated use. Rates of graft success were higher in heterografts between `Suncrest' bases and tips of `Springcrest' or `Nemaguard' than in autografts between `Suncrest' and `Suncrest', and there was equal efficacy of graft inoculation from `Suncrest' into these three cultivars.

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