Structural analysis of morphometric and morphofunctional state of immunocompetent blood cells  in case of purulent inflammatory diseases of soft tissues on the background of type 2 diabetes

Relevance. According to the study’s results, steady changes in various parts of the immune system: cellular, humeral, phagocytic activity is revealed in patients with type 2 diabetes. Hyperglycemia, hyperlipidemia, insulin resistance, and adaptive ehyperinsulinemia affect the cells of the immune system, promoting the development of metabolic immunosuppressant and forming a stable immunological disorder. The purpose of the study into the morphometric and morphofunctional state of peripheral blood leukocytes in patients with type 2 diabetes to determine their significance in the development of purulent-inflammatory diseases of soft tissues are research. Materials and methods. In this work, the analysis of morphometric, cytogystochemical and laboratory study results of the control (20 healthy volunteers) and thematic (47 patients with supportive soft tissue disease son the background of type 2 diabetes mellitus) study groups with the statistical processing of the obtained results was used. Results. The investigation of morphofunctional features of nonspecific and specific protection cells for purulent inflammatory diseases of tissues on the background of type 2 diabetes showed that the basis of diabetic complications are there vealed changes in the bactericidal activity of segmented neutrophils. The trend of indicators of leukocyte formula and the distribution curve of mononuclear cells by size, as well as the growth of the lymphocyticgranulocytic index, indicate a chronic formation of endotoxin synthesize of diabetes. Conclusions. Lowering the level of cationic proteins, the activity of myeloperoxidase and the NBT-test under stimulation of neutrophil granulocytes bylectinsis a sign of unsatisfactory state of the bactericidal cells system, which may be the cause of chronic and acute inflammatory processes in the body that accompany type 2 diabetes, to a functional exhaustion of the macrophage link in the organism immune defense. Keywords: type 2 diabetes, purulent-inflammatory diseases of soft tissues, immunogenesis, cytomorphometry, cytogistochemistry, immunocompetent blood cells.

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Results of cytochemical investigation of neutrophil granulocytes in patients with purulent-inflammatory diseases of fine tissues on the type 2 diabetes mellitus

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2018-22(3)-14 ◽

2018 ◽

Vol 22 (3) ◽

pp. 468-470

Author(s):

M.D. Zheliba ◽

M.H. Bohachuk

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Immune System ◽

Soft Tissues ◽

Inflammatory Diseases ◽

Main Group ◽

Neutrophil Granulocytes ◽

Inflammatory Processes ◽

Stimulation Of

Patients with purulent-inflammatory diseases of soft tissues on the background of diabetes type 2 are resistant changes in various parts of the immune system. Hyperglycemia, hyperlipidemia, insulin resistance and adaptive hyperinsulinemia affect the cells of the immune system, promote the development of metabolic immunosuppression with the formation of stable immune dysfunction. The purpose of the study to investigate and analyze the metabolic state of immunocompetent cells in patients with purulent-inflammatory diseases of soft tissues on the background of DMD type 2. The study was conducted in 47 patients (the main group) with purulent-inflammatory diseases of soft-tissue on the background of diabetes mellitus, and 20 healthy volunteers (the comparison group) using the cytohistochemical restoration method of NST based on the percentage composition of neutrophils that have cytoplasm of formazan granules. The spontaneous neutrophil response was studied, as well as the parameters of the NST-test in stimulation of neutrophil granulocytes in vitro to assess the bactericidal, resource potential and their ability to complete phagocytosis. Results of the study showed that the decrease in the activity of myeloperoxidase and the NST-test in the conditions of stimulation of neutrophil granulocytes is a sign of unsatisfactory state of the bactericidal system of cells, which may be the cause of acute inflammatory processes in the body that accompany type 2 diabetes, which leads to functional exhaustion of the macrophage level of the immune body protection. At GZZMT against the background of DM 2 type compared with the norm, the “spontaneous” NST-test of neutrophils is high, which is due to the presence of inflammatory process. The index of stimulation of neutrophils is lower in the main group (1.6 times: 5.3±0.2% in the main group versus 8.4±0.4% in the control, p≤0.05), which is an indicator of a relatively weaker stimulation of those populations of neutrophils that were responsible for high indices in the “spontaneous” NST test, and the impaired state of cellular metabolism. So, in patients with type 2 diabetes, a violation of the bactericidal system of organism protection, which is one of the causes of the development of infectious and inflammatory processes.

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Structural analysis of tooth and jawbone in a type 2 diabetes mouse model

Bone Abstracts ◽

10.1530/boneabs.3.pp26 ◽

2014 ◽

Author(s):

Silvia Pabisch ◽

Tsuguno Yamaguchi ◽

Yasushi Koike ◽

Kenji Egashira ◽

Shinsuke Kataoka ◽

...

Keyword(s):

Type 2 Diabetes ◽

Structural Analysis ◽

Mouse Model ◽

Diabetes Mouse

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Decubitus Ulcers of Soft Tissues in Patients with Type 2 Diabetes Mellitus: Clinical Strategies, Insulin Resistance Indicators, Comprehensive Treatment Aspects

INTERNATIONAL JOURNAL OF ENDOCRINOLOGY ◽

10.22141/2224-0721.5.77.2016.78758 ◽

2016 ◽

Vol 0 (5.77) ◽

pp. 72

Author(s):

A.R. Vergun ◽

M.R. Krasnyi ◽

Ya.B. Chulovsky ◽

O.M. Vergun ◽

O.M. Moshchynska

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Soft Tissues ◽

Comprehensive Treatment ◽

Decubitus Ulcers ◽

Clinical Strategies

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Role of Circulating Microparticles in Type 2 Diabetes Mellitus: Implications for Pathological Clotting

Seminars in Thrombosis and Hemostasis ◽

10.1055/s-0041-1740150 ◽

2021 ◽

Author(s):

Siphosethu Cassandra Maphumulo ◽

Etheresia Pretorius

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Systemic Inflammation ◽

Blood Cells ◽

Low Grade ◽

Chronic Hyperglycemia ◽

Increased Risk

AbstractType 2 diabetes mellitus (T2DM) is a multifactorial chronic metabolic disease characterized by chronic hyperglycemia due to insulin resistance and a deficiency in insulin secretion. The global diabetes pandemic relates primarily to T2DM, which is the most prevalent form of diabetes, accounting for over 90% of all cases. Chronic low-grade inflammation, triggered by numerous risk factors, and the chronic activation of the immune system are prominent features of T2DM. Here we highlight the role of blood cells (platelets, and red and white blood cells) and vascular endothelial cells as drivers of systemic inflammation in T2DM. In addition, we discuss the role of microparticles (MPs) in systemic inflammation and hypercoagulation. Although once seen as inert by-products of cell activation or destruction, MPs are now considered to be a disseminated storage pool of bioactive effectors of thrombosis, inflammation, and vascular function. They have been identified to circulate at elevated levels in the bloodstream of individuals with increased risk of atherothrombosis or cardiovascular disease, two significant hallmark conditions of T2DM. There is also general evidence that MPs activate blood cells, express proinflammatory and coagulant effects, interact directly with cell receptors, and transfer biological material. MPs are considered major players in the pathogenesis of many systemic inflammatory diseases and may be potentially useful biomarkers of disease activity and may not only be of prognostic value but may act as novel therapeutic targets.

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