scholarly journals Nghiên cứu điều kiện tiền xử lý và chiết tách collagen từ da cá lóc (Channa striata) bằng pepsin

2021 ◽  
Vol 57 (6) ◽  
pp. 178-188
Author(s):  
Thị Mộng Thu Trương ◽  
Đỗ Quỳnh Nguyễn ◽  
Thanh Trúc Trần ◽  
Thị Minh Thủy Lê

Nghiên cứu điều kiện tiền xử lý và chiết tách collagen từ da cá lóc bằng pepsin đã được thực hiện. Kết quả cho thấy da cá lóc được xử lý với 10% butyl alcohol trong 72 giờ thì hàm lượng lipid còn lại thấp nhất là 15,3%. Collagen từ da cá lóc được chiết tách với 0,45% pepsin trong 24 giờ cho hiệu suất thu hồi 13,7% và độ hòa tan cực đại ở pH  1 - 4 và nồng độ NaCl từ 0,2 - 0,6 M. Bên cạnh đó, phổ FTIR cho thấy mối quan hệ chặt chẽ giữa số bước sóng trong vùng amide I và vùng amide III đặc biệt là sự ổn định của cấu trúc xoắn ba, cho thấy collagen từ da cá lóc có đầy đủ nhóm chức năng của collagen loại I. Collagen có màu sáng với giá trị L* là 62,4 và hàm lượng imino acid là 204 (đơn vị/1000 đơn vị). Kết quả nghiên cứu cho thấy có thể sử dụng pepsin để thay thế hoá chất nhằm giảm thiểu lượng hoá chất thải ra môi trường, tận dụng da cá lóc như nguồn nguyên liệu để sản xuất collagen.

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1188
Author(s):  
Thi Mong Thu Truong ◽  
Van Muoi Nguyen ◽  
Thanh Truc Tran ◽  
Thi Minh Thuy Le

The isolation of acid-soluble collagen (ASC) from by-products of snakehead fish (Channa striata), including skin and the mixture of skin and scale, has been investigated. The recovery yield of fish skin ASC (13.6%) was higher than ASC from fish skin and scale (12.09%). Both ASCs were identified as type I collagen and showed maximal solubility at pH 2. Collagen samples from the mixture of skin and scale had higher imino acid content (226 residues/1000 residues) and lower wavenumber in the amide I and amide III region (1642 and 1203 cm−1, respectively) than the fish skin ASC (the imino acid content was 220 residues/1000 residues and the wavenumber in the amide I and amide III were 1663 and 1206 cm−1, respectively. The difference scanning calorimeter (DSC) showed higher thermal stability in ASC from the mixture of skin and scale (Td of 35.78 °C) than fish skin ASC (34.21 °C). From the result, the denaturation temperature of ASC had a close relationship with the content of imino acid as well as with the degradation of α-helix in amide I and III. These results suggest that collagen could be obtained effectively from snakehead fish by-products and has potential as a realistic alternative to mammalian collagens.


2020 ◽  
Vol 3 (1) ◽  
pp. 10
Author(s):  
Muhammad Noor Yasin ◽  
Firlianty . ◽  
Anang Najamudin
Keyword(s):  
Omega 3 ◽  
Omega 6 ◽  

Eccado adalah produk olahan yang dibuat dari daging atau udang yang dicincang dengan penambahan tepung dan bumbu-bumbu. Pada umumnya Eccado diolah dari daging ayam, untuk menggantikan eccado ayam maka dicari sumber alternatif baru salah satunya digunakan ikan Gabus karena memiliki daging yang banyak, tebal dan putih. Potensi eccado ikan Gabus ini sangat tinggi baik aspek ekonomi juga kandungan gizinya karena mengandung omega 3 dan omega 6. Ikan Gabus bisa dimanfaatkan dengan cara langsung mengolahnya menjadi beberapa jenis makanan atau mengawetkannya melalui proses penggaraman dan pengeringan. Masih belum banyaknya diversifikasi bentuk pengolahan ikan hasil usaha budidaya khususnya ikan Gabus menjadi makanan olahan lain juga disebabkan karena masih minimnya informasi tentang teknologi pengolahan hasil perikanan. Permasalahan yang dihadapi mitra adalah : 1) Selama ini mitra belum mengetahui variasi tentang Teknologi Hasil Perikanan karena terbatasnya pengetahuan dan informasi yang diterima serta keterampilan yang dimiliki. 2) Mitra juga belum pernah mendapatkan pelatihan dan demonstrasi tentang pembuatan eccado dari ikan air tawar dalam hal ini ikan jenis ikan family Channidae Menurut SNI 7756 : 2013 dapat didefinisikan Eccado ikan merupakan produk olahan hasil perikanan dengan menggunakan lumatan daging ikan/udang dan atau surimi minimum 30%, tepung dan bahan-bahan lainnya, dibentuk dan dibungkus dengan kulit pangsit. Sedangkan Eccado merupakan salah satu jenis olahan fish jelly produk dengan menggunakan kulit kembang tahu yang berfungsi memberikan rasa yang khas.Tujuan kegiatan pengabdian kepada masyarakat ini untuk membantu mengatasi permasalahan yang dihadapi oleh masyarakat Pahandut Seberang l) Pendampingan teknologi diversifikasi pengolahan daging ikan menjadi eccado. 2) Promosi kegiatan Pahandut seberang sebagai lokasi wisata kuliner dengan kegiatan-kegiatan yang dapat meningkatkan kualitas kuliner dari ikan dan ekonomi masyarakat di sekitarnya.


2018 ◽  
Vol 16 (2) ◽  
pp. 219-226
Author(s):  
Sri Rizqi Annisa ◽  
Dewi Larasati ◽  
Endang Bekti K

The aim of this study was to determine the characterization of shredded mureel fish with kluwih substitution on water content, protein content, fiber content and organoleptic (preference for crispness and taste). This study uses a simple Randomized Complete Design (RCD) with the substitution treatment of kluwih and mureel fish, with the following ratio: S1 (240g: 60g), S2 (210g: 90g), S3 (180g: 120g), S4 (150g: 150g), S5 (120g: 180g). Data were analyzed statistically by analysis of variance and if there was a significant effect, further testing was done with BNJ at the level of 5%. The results showed that kluwih substitution in the manufacture of mureel fish shredded had an average: water content of 8.33-10.62%, protein :16.83-22.00%, fiber : 6.79-6.99%, score crispness 2-6.12, taste score 2.6-6.6. Based on the results of the analysis of the variety of kluwih substitutes and mureel fish have a significant effect on water content, protein content and crisp organoleptic test, taste on mureel fish fillet, and no significant effect on fiber content. The best kluwih substitution in S3 treatment with 120 grams of substitute kluwih and 180 grams of mureel fish.


2019 ◽  
Vol Tập 55, Số 2 ◽  
pp. 88
Author(s):  
Trần Thị Phương Lan ◽  
Trần Thị Thanh Hiền ◽  
Lam Mỹ Lan
Keyword(s):  

2019 ◽  
Vol 55(Công nghệ Sinh học) ◽  
pp. 301
Author(s):  
Trần Bạch Long ◽  
Trần Thanh Trúc ◽  
Nguyễn Văn Mười
Keyword(s):  

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