scholarly journals Effect of Probiotic Lactobacillus sp. Dad13 on Humoral Immune Response of Balb/C Mice Infected with Salmonella typhimurium

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Ika Dyah Kusumawati ◽  
Eni Harmayani ◽  
Widya Asmara
Keyword(s):  
Immune Response ◽  
Salmonella Typhimurium ◽  
Humoral Immune Response ◽  
Buffalo Milk ◽  
Secretory Iga ◽  
Standard Diet ◽  
Salmonella Infection ◽  
Humoral Immune ◽  
Probiotic Lactobacillus

An indigenous strain of lactic acid bacterium (LAB) identified as Lactobacillus spp. Dad13 (Dad13), isolatedfrom traditional fermented buffalo milk, was found to be potential as probiotic. The aim of this research was to studythe effect of probiotic Dad13 on humoral immune response of Balb/C mice infected with Salmonella typhimurium. Thespecific objective was to find out the effect of different Dad13 consumption time (before and along with infection of S.typhimurium) on the humoral immune response of Balb/C mice. The experiment was conducted by in vivo trial on 20males of Balb/C mice, age of 6-8 weeks, fed with AIN-93 standard diet. The mice were assigned into 4 groups. Eachgroup received the following treatments, ie. Dad13 only, Dad13 before infection, Dad13 along with infection andSalmonella infection only. A volume of 100 μl Dad13 cell suspensions (1010 CFU/ml) were given by oral forced feedingdaily for a week, at week 3 for group before infection and at week 4 for group of Dad13 only and Dad13 along withinfection. Salmonella infection (109 CFU/ml) was given once orally at week 4 to all groups except group treated withDad13 only. The humoral immune response of Balb/C mice was detected 2 weeks after infection by measuring thetiters of IgG and IgA specific from serum and mucosal intestinal liquid samples using Enzyme-linked ImmunosorbentAssay (ELISA) method. The result indicated that humoral immune response of Balb/C mice consuming Dad13 beforeand along with Salmonella infection were significantly different (p<0.05). Dad13 consumption along with Salmonellainfection increased circulated IgG and IgA as well as secretory IgA. It can be concluded that Dad13 probiotic feedingalong with infection increased humoral immune response more significantly compared to that before infection.Key words : Probiotic, Lactobacillus sp. Dad13, Immune response, Salmonella typhimurium

scholarly journals EXOSOMES DISPLAYING HIGH EXPRESSION OF CD24 AND HSP90 INDUCED CELLULAR AND HUMORAL IMMUNE RESPONSE IN VIVO

2013 ◽  
Vol 4 ◽  
Author(s):  
Mongini Claudia ◽  
Hershlik Leticia ◽  
Di Sciullo Paula ◽  
Menay Florencia ◽  
Gravisaco Mar�a ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
High Expression ◽  
Humoral Immune

scholarly journals CRTC2 regulates plasma cell metabolism and survival

2021 ◽  
Author(s):  
Jason Hong ◽  
Fasih Ahsan ◽  
Encarnacion Montecino-Rodriguez ◽  
Peter Pioli ◽  
Min-sub Lee ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Plasma Cells ◽  
Cell Metabolism ◽  
Active Form ◽  
B Cell Differentiation ◽  
Antibody Secreting Cell ◽  
Humoral Immune

Antibody secreting cell (ASC) function and longevity determines the strength and durability of a humoral immune response. Previously, we identified the inactivation of the CREB-regulated transcriptional coactivator-2 (CRTC2) in an in vitro B cell differentiation assay that produced functional ASCs. However, the requirement for CRTC2 inactivation in ASC physiology in vivo remains unknown. Using transgenic (TG) mice that express a constitutively active form of CRTC2 (Crtc2-AA) as an experimental tool, we demonstrate that Crtc2 repression in plasma cells (PCs) is an intrinsic requirement for ASC metabolic fitness. Sustained CRTC2 activity shortens the survival of splenic and bone marrow PCs, resulting in reduced numbers of long-lived PCs and antibody deficits against T cell dependent and independent antigens, and an acute viral infection. TG PCs resemble short-lived PCs with reductions in glycolysis, oxidative metabolism, spare respiratory capacity, and antibody secretion. Mechanistically, Crtc2 repression is necessary for the fidelity of PC gene expression and mRNA alternative-splicing programs. Combined, Crtc2 repression in PCs must occur to support PC metabolism and extend ASC survival during a humoral immune response.

scholarly journals Humoral immune response of Salmonella typhimurium and Salmonella enteritidis sonicated antigens in rabbits

2012 ◽  
Vol 36 (0E) ◽  
pp. 84-88
Author(s):  
Ekram A. Al-Samarrae
Keyword(s):  
Immune Response ◽  
Salmonella Typhimurium ◽  
Humoral Immune Response ◽  
Salmonella Enteritidis ◽  
Agglutination Test ◽  
Control Group ◽  
Whole Cell ◽  
Humoral Immune ◽  
The Third ◽  
Infected Goat

Salmonella typhimurium and salmonella enteritidis were isolated from infected goat andprepared an antigens of whole cell sonicated antigen of S.typhimurium(WCS.Ag.S.typhimurium ),whole cell sonicated antigen of S.enteritidis (WCS.Ag.S.entertidis) and combination of whole cell sonicated antigen (Salmonella typhimurium andSalmonella enteritidis) (CWS.Ag) . Their efficacy was evaluated by using tube agglutinationtest and enzyme linked immune sorbent assay (ELISA). Twenty rabbits were randomlydivided into four groups; the 1st group was immunized by WCS. Ag - Salmonella enteritidis,2nd group immunized by (WCS Ags .typhimurium), 3rd group immunized by CWCS.Agcompound and 4th left as control group which injected by physiological buffer saline (pH7.2). The antibody titer was increased in after the day 12, first, second and third months ofimmunization by agglutination test. IgG concentration was done by ELISA at the same time;which were recorded a higher significant differences (p˂ 0.01) at the first month in the groupimmunized by CWS Ag (449.65 ±38.6 1ng/ml IgG and 952± 20.85 antibodies titer )compared with other immunized groups ( WCS – Ag – S. enteritidis andWCS.Ag.S.typhimurium ). Also, the IgG concentration and antibodies titer are still higher inthe second and the third months in the immunized group by CWCS.Ag. 218.90± 6.69ng/ml,528± 68.58 and 89.55± 2.63ng/ml, 280± 49.98 respectively with significant differences (p˂0.01) compared with the immunized groups (WCS.Ag.S. entertidis and WCS. Ag.S.typhimurium) and also, they are significant (p˂ 0.01) when compared with the control groupResearch

Sign in / Sign up

Export Citation Format

Share Document