scholarly journals EXTRACTION OF PALM OIL’S FREE FATTY ACIDS BY TRIETHYLAMMINE (TEA) IN POLAR-NONPOLAR MIX-SOLVENT

2010 ◽  
Vol 2 (1) ◽  
pp. 48-54
Author(s):  
Endang Astuti ◽  
Winarto Haryadi ◽  
Sabirin Matsjeh
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Diethyl Ether ◽  
Palm Oil ◽  
Standard Method ◽  
Acid Value ◽  
Water Volume ◽  
Acid Extraction

Palm oil contains unsaturated fatty acids that can be oxidated and can make rancidity of the palm oil. One of the trigliserida degradation products is free fatty acid. The usage of triethylammine (TEA) in the polar-nonpolar mix solvents could enhance the free fatty acids extraction efficiency. Free fatty acids extraction from palm oil was carried out for a minute with solvents ratio: TEA 0,00-0,20M in the following solvent: 0-99% diethyl ether + 99-0% ethanol + 1% water, % volume. The extraction was also performed in the following solvent: TEA 0,00-0,20M in 0-95% heptane + 95-0% isopropanol + 5% water, % volume. Reextraction was conducted for a minute by adding Na2SO4 1M. The alcohol layer which was rich with free fatty acid was taken out and the acid value was determined. As comparison, palm oil's free fatty acids was taken out with standard method by refluxing the sample in 95% alcohol for ten minutes. Identification of the free fatty acids was done by Gas Chromatography-Mass Spectrometer. The best solvent ratio for acid value determination was TEA 0,10-0,20M in the mix-solvent: 19% diethyl ether + 80% ethanol + 1% water, % volume and the extraction product contained 8 kind of free fatty acids. In addition, TEA 0,01M in the solvent: 95% isopropanol + 5% water, % volume could be obtained 8 kind of free fatty acids too. In contrary, the free fatty acid extraction by standard method contained 7 kind of fatty acids. Linoleic acid was the free fatty acid which could not be taken out by standard method. The usage of TEA in the proper polar-non polar mix-solvent was the complete method to determine free fatty acid contained in the palm oil.     Keywords: triethylammine, free fatty acid, organic extraction.

Separation of free fatty acids from high free fatty acid crude palm oil using short-path distillation

2016 ◽  
Author(s):  
Abd Al-Wali Japir ◽  
Jumat Salimon ◽  
Darfizzi Derawi ◽  
Murad Bahadi ◽  
Muhammad Rahimi Yusop
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Palm Oil ◽  
Short Path ◽  
High Free Fatty Acid ◽  
Crude Palm Oil ◽  
Short Path Distillation

scholarly journals Penghambatan Peningkatan Kadar Asam Lemak Bebas (Free Fatty Acid) pada Buah Kelapa Sawit dengan Menggunakan Asap Cair

2017 ◽  
Vol 9 (2) ◽  
pp. 44-49 ◽  
Author(s):  
Teuku Maimun ◽  
Nasrul Arahman ◽  
Fikriatul Arifah Hasibuan ◽  
Putri Rahayu
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Palm Oil ◽  
Oil Palm ◽  
Crude Palm Oil ◽  
Palm Fruit ◽  
Liquid Smoke ◽  
Oil Hydrolysis

Abstract                                                                        One cause of the increase in free fatty acids (FFA) in Crude Palm Oil (CPO) is the activity of lipase-producing microorganisms in the oil palm fruit. Lipase is a biocatalisator which accelerate oil-hydrolysis reaction. High Free Fatty Acid Levels (FFA) will caused rancidity, change the taste and color of the oil. To solve this problem, post-harvest palm fruits should treat with some special treatment, e.g. addition of anti-microbial material, such as liquid smoke The purpose of this study was to determine the effect of liquid smoke on oil palm fruit, the inhibition of elevated levels of free fatty acids (FFA) on CPO. The factors reviewed in this study is the concentration of liquid smoke used; 5, 15, and 25% and the standing time; 12, 16 and 20 hours. The parameters observed in this study are free fatty acids (FFA), water, and impurities. The results showed that the higher the concentration of liquid smoke added, the higher inhibition of oil hydrolysis. CPO levels of FFA decreased at approximately 0,03 to 0,37% after the addition of liquid smoke. Highest inhibition on ALB increase was obtained in the treatment of the sample with the addition of 25% liquid smoke and 12 hours of standing time, which is 2,46%. Keywords: Crude Palm Oil (CPO), Free Fatty Acid (FFA), Water, Impurities, Liquid Smoke.Abstrak       Salah satu penyebab peningkatan asam lemak bebas (ALB) di dalam Crude Palm Oil (CPO) adalah, karena adanya aktivitas mikroorganisme penghasil lipase di dalam buah kelapa sawit. Lipase merupakan biokatalisator yang mempercepat reaksi hidrolisis minyak. Kadar Asam Lemak Bebas (ALB) yang tinggi menyebabkan ketengikan, perubahan rasa dan warna pada minyak. Untuk mengatasi masalah ini, buah kelapa sawit perlu diberi perlakuan khusus, yaitu penambahan bahan anti mikroba pada buah kelapa sawit pasca panen, salah satunya adalah asap cair. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan asap cair pada buah kelapa sawit, terhadap penghambatan peningkatan kadar asam lemak bebas (ALB) pada CPO. Adapun faktor yang ditinjau dalam penelitian ini adalah konsentrasi asap cair yang digunakan, yaitu 5, 15, dan 25% dan waktu pendiaman, yaitu 12, 16, dan 20 jam. Parameter yang diamati dalam penelitian ini antara lain, asam lemak bebas (ALB), air, dan kotoran. Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi asap cair yang ditambahkan, maka penghambatan hidrolisis minyak semakin tinggi. Kadar ALB pada CPO yang dihasilkan menurun sekitar 0,62 – 2,55% setelah penambahan asap cair. Penghambatan peningkatan ALB tertinggi diperoleh pada perlakuan sampel dengan penambahan 25% asap cair dan waktu pendiaman 12 jam, yaitu 2.46%. Kata kunci : Crude Palm Oil (CPO), Asam Lemak Bebas (ALB), Air, Kotoran (Impuritis), Asap cair (Liquid Smoke)

scholarly journals THE IMMOBILIZATION OF LIPASE FROM MUCOR MIEHEI ON ZEOLITE MATRIX IN HYDROLYSIS OF PALM OIL PRODUCING FREE FATTY ACIDS WITH SOLVENT FREE SYSTEM

2018 ◽  
Vol 7 (2) ◽  
pp. 108-114
Author(s):  
Dwina Moentamaria ◽  
Achmad Chumaidi ◽  
Nanik Hendrawati ◽  
Girlian Girlian ◽  
Meilita Againa Mustika
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Palm Oil ◽  
Immobilized Lipase ◽  
Mucor Miehei ◽  
Oil Hydrolysis ◽  
Hydrolysis Of ◽  
Free Lipase

The enzymatic hydrolysis of palm oil can be conducted by using lipase produced from Mucor miehei to produce free fatty acid. This study aimed to compare the usage of lipase as free enzyme and as immobilized enzyme on zeolite matrix in the hydrolysis of palm oil as triglyceride producing free fatty acids which highly needed in various industrial sectors. Immobilization is an alternative hydrolysis reaction due to its usage on repetitive reaction, makes lipase reuseable, hence the whole process becomes efficient, and with moderate operational conditions. Solvent free reaction is applied, because the produced free fatty acids can be used directly in food, health, and natural flavorings industry. The palm oil used in the hydrolysis contains 0.815% initial free fatty acids as palmitate, in which water then added to it in weight ratio 1:3. Each effect of free lipase and immobilized lipase addition is 4%, 5%, 6%, 7%, 8%, and time reaction is 30, 60, 90, 120, 150 minutes are used as index to determine the amount of free fatty acids produced.  The results showed that Immobilized lipase has better ability than the free one in hydrolysis of triglyceride in palm oil producing free fatty acid with 8% lipase addition and time reaction of 120 minutes. Palm oil hydrolysis using free lipase produced the highest FFA of 1.9747% after the addition of 5% lipase concentrate, with time reaction of 60 minutes. Meanwhile, palm oil hydrolysis using immobilized lipase produced the highest FFA of 1.9747% after the addition of 8% lipase concentrate, with time reaction of 120 minutes.

Effect of insulin on free fatty acid uptake by hepatocytes in the duck

1984 ◽  
Vol 102 (3) ◽  
pp. 381-386 ◽  
Author(s):  
R. Gross ◽  
P. Mialhe
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Glucose Metabolism ◽  
Free Fatty Acids ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Low Doses ◽  
Higher Doses

ABSTRACT To elucidate the hypolipacidaemic effect of insulin in ducks, its action on the uptake of free fatty acids (FFA) by duck hepatocytes was determined. At low doses (10 mu./l) insulin stimulated FFA uptake. This effect was not observed with higher doses of insulin (20, 30 and 50 mu./l). Growth hormone at physiological concentrations and corticosterone (14·4 nmol/l) decreased basal activity, probably by reducing glucose metabolism and consequently α-glycerophosphate (α-GP) supply. Insulin was able to reverse the inhibition induced by GH and corticosterone on both FFA uptake and α-GP production. These results therefore suggest that the hypolipacidaemic effect of insulin may be partly mediated by its action on hepatic FFA uptake. J. Endocr. (1984) 102, 381–386

scholarly journals Free Fatty acids receptors (FFAR2 and FFAR3) control cell proliferation by regulating cellular glucose uptake

2019 ◽  
Author(s):  
Mohammad Aziz ◽  
Saeed Al Mahri ◽  
Amal Alghamdi ◽  
Maaged AlAkiel ◽  
Monira Al Aujan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Cell Proliferation ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Glucose Uptake ◽  
Microarray Data ◽  
Free Fatty Acid Receptors

Abstract Background Colorectal cancer is a worldwide problem which has been associated with changes in diet and lifestyle pattern. As a result of colonic fermentation of dietary fibres, short chain free fatty acids are generated which activate Free Fatty Acid Receptors 2 and 3 (FFAR2 and FFAR3). FFAR2 and FFAR3 genes are abundantly expressed in colonic epithelium and play an important role in the metabolic homeostasis of colonic epithelial cells. Earlier studies point to the involvement of FFAR2 in colorectal carcinogenesis. Methods Transcriptome analysis console was used to analyse microarray data from patients and cell lines. We employed shRNA mediated down regulation of FFAR2 and FFAR3 genes which was assessed using qRT-PCR. Assays for glucose uptake and cAMP generation was done along with immunofluorescence studies. For measuring cell proliferation, we employed real time electrical impedance based assay available from xCelligence. Results Microarray data analysis of colorectal cancer patient samples showed a significant down regulation of FFAR2 gene expression. This prompted us to study the FFAR2 in colorectal cancer. Since, FFAR3 shares significant structural and functional homology with FFAR2, we knocked down both these receptors in colorectal cancer cell line HCT 116. These modified cell lines exhibited higher proliferation rate and were found to have increased glucose uptake as well as increased level of GLUT1. Since, FFAR2 and FFAR3 signal through G protein subunit (Gαi), knockdown of these receptors was associated with increased cAMP. Inhibition of PKA did not alter the growth and proliferation of these cells indicating a mechanism independent of cAMP/PKA pathway. Conclusion: Our results suggest role of FFAR2/FFAR3 genes in increased proliferation of colon cancer cells via enhanced glucose uptake and exclude the role of protein kinase A mediated cAMP signalling. Alternate pathways could be involved that would ultimately result in increased cell proliferation as a result of down regulated FFAR2/FFAR3 genes. This study paves the way to understand the mechanism of action of short chain free fatty acid receptors in colorectal cancer.

Epinephrine-insulin antagonism on free fatty acid release

1961 ◽  
Vol 201 (5) ◽  
pp. 815-818 ◽  
Author(s):  
John J. Spitzer ◽  
William T. McElroy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Blood Sugar ◽  
Drug Administration ◽  
Hepatic Uptake ◽  
Fatty Acid Release ◽  
Plasma Ffa ◽  
Acid Release

The effects of epinephrine or norepinephrine were studied in dogs receiving insulin plus glucose prior to and during administration of the amine. Epinephrine caused a significantly smaller elevation of free fatty acids (FFA) with than without insulin plus glucose administration. Blood sugar responses were quantitatively similar. Epinephrine increased both hepatic uptake of FFA and hepatic release of glucose; these changes were similar to the ones found previously in dogs not receiving insulin plus glucose. The action of norepinephrine on elevating plasma FFA was only slightly and not significantly affected by the administration of insulin plus glucose. When the order of drug administration was reversed, infusion of insulin plus glucose lowered plasma FFA levels and hepatic FFA uptake in animals already receiving either epinephrine or nonepinephrine.

Changes in plasma free fatty acid concentrations on passage through the dog kidney

1961 ◽  
Vol 200 (5) ◽  
pp. 1095-1098 ◽  
Author(s):  
Frank J. Hohenleitner ◽  
John J. Spitzer
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Renal Plasma Flow ◽  
Plasma Free Fatty Acid ◽  
Systemic Artery ◽  
Arterial Concentration ◽  
Dog Kidney ◽  
Anesthetized Dogs

To measure the renal removal of free fatty acids from the plasma, simultaneous determinations of this metabolite were performed in a systemic artery and a renal vein in the anesthetized dogs. Renal plasma flow was also determined by the PAH method, and the renal uptake of free fatty acids was calculated. Concentrations of free fatty acids in renal venous plasma were usually lower than the arterial concentrations. The arteriovenous differences were statistically highly significant. The results also suggested that the degree of free fatty acid removal was proportional to the arterial concentration of this metabolite.

Free fatty acid concentration and composition in arterial blood

1962 ◽  
Vol 203 (2) ◽  
pp. 306-310 ◽  
Author(s):  
Martin E. Rothlin ◽  
Christine B. Rothlin ◽  
Vernon E. Wendt
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Free Fatty Acid Concentration ◽  
Arterial Blood ◽  
Arterial Concentration ◽  
The Subject ◽  
Palmitic Acids

The effect of the administration of norepinephrine, glucose and insulin, pentobarbital, and Hypertensin on the arterial concentration and composition of plasma free fatty acids (FFA) has been studied in man and dog. With a rise of the FFA concentration as produced by norepinephrine, the contribution of oleic acid to the total FFA increased, while that of stearic and palmitic acids decreased. The reverse changes in the FFA composition were observed when their arterial level fell under the influence of other agents studied. The FFA composition was dependent on the FFA concentration in arterial blood, but not on the experimental condition of the subject or animal at the time of analysis. At high FFA levels, the FFA composition approached that of depot fat.

Reaction of Free Fatty Acids with Protein in Cod Muscle Frozen and Stored at −29 C after Aging in Ice

1969 ◽  
Vol 26 (10) ◽  
pp. 2727-2736 ◽  
Author(s):  
Margaret L. Anderson ◽  
Elinor M. Ravesi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Complex Formation ◽  
Free Fatty Acids ◽  
Phase Contrast ◽  
Reaction Rate ◽  
Frozen Storage ◽  
Phase Contrast Micrographs ◽  
Protein Extractability

Freezing and holding cod muscle in the frozen state favored the association process that involves protein–free fatty acid (FFA) complex formation and begins during aging in ice. Changes in protein extractability, in ultracentrifugal patterns of protein extracted, and in phase contrast micrographs of inextractable muscle fragments were followed in muscle that had been aged in ice to produce various contents of FFA and then frozen and held at −29 C. After 11 months, these changes, which took place largely during the first week of storage, were comparable with those that occur when the FFA are formed during frozen storage. The results were consistent with a reaction rate that was greater at −29 C than at temperatures a few degrees above 0 C.

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