APLIKASI MINYAK CENGKEH SEBAGAI OBAT BIUS PADA KAKAP PUTIH  (Lates calcarifer Bloch)

The objective of this study was to determine the safety and efficacy of clove oil as an anesthetic in sea bass (Lates calcarifer) and the potential application of clove oil as anesthetic to facilitate the sea bass fry transportation. Acute toxicity test indicated the 24-hr LC50 value of clove oil in sea bass fry as 30 ppm with slope function of 1.079 (1.05 to 1.107). In efficacy test, fish were exposed to 5, 10, 15, 20 and 25 ppm of clove oil for 15 minutes. At 5 ppm, clove oil caused only sedation effect (partial loss of reaction to external stimuli) while at 20 ppm, fish entered anesthesia stage (failure to respond to external stimuli) within about 3 minutes. Fish recovered from a 15-min period of exposure in 20 ppm clove oil within less than 10 minutes following removal from the anesthetic solution. There was neither mortality nor abnormal behavior of fish during 15-min exposure of clove oil as well as during 7 days post recovery from anesthesia. The potential application of clove oil as an aid in the transport of sea bass fry in plastic bag was also investigated. At 5 ppm, clove oil could reduce activities of the fish without loss of equilibrium (sedation stage) during the 4 hour simulated transport at 50 fish per 1,000 ml sea water (15 ppt). At 20 ppm, clove oil caused loss of equilibrium in fish resulting in the anesthesia stage throughout the 4 hour period. However, there was no improvement on survival rate and fish behavior with the use of clove oil during and after this 4 hour transport. Simulated transport at 50 fish per 500 ml sea water (15 ppt) for 8 hour did show better significant survival rate with additional of 5 and 20 ppm clove oil. In both short and long term transport study, clove oil did show the benefit by reducing the fish activities judging from the reduction of oxygen consumption, ammonia and carbon dioxide levels. Addition of appropriate concentration of clove oil in transport water ensured that the fish would stay calm by reducing fish activity and therefore, prevented any drastic changes of water qualities.

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Growth Performance and Survivability of the Asian Seabass Lates Calcarifer Reared Under Hyper-Saline, Hypo-Saline and Freshwater Environments in a Closed Aquaculture System

10.21203/rs.3.rs-211072/v1 ◽

2021 ◽

Author(s):

Habib Ul Hassan ◽

Qadeer Mohammad Ali ◽

Zubia Masood ◽

Mohammad Abdul Momin Siddique

Keyword(s):

Survival Rate ◽

Growth Performance ◽

Feeding Rate ◽

Sea Bass ◽

Salinity Treatment ◽

Lates Calcarifer ◽

Asian Seabass ◽

Treatment Groups ◽

Health Indices ◽

Asian Sea Bass

Abstract Salinity is one of the most critical environmental parameters regarding fish physiology, modifying food intake and growth performance in many fish species. The present study has investigated the effects of different salinity levels on growth performance, feeding and survival of Asian seabass Lates calcarifer juveniles. Asian seabass juveniles were reared at 0, 5, 22, 36, and 42 ppt salinity. One five hundred ninety-eight fish individuals with an average weight 1.2 ± 0.11 g were randomly distributed (166 fish/Tank) in 5 concrete tanks (30×6×4 feet) each; length × width × depth) and volume 19,122 L, for total forty days. Initially feeding rate of 6% biomass per day with 42% crude protein-containing diets daily. The feeding rate adjusted according to fish biomass every week. The results showed that salinity level had a significant effect on the weight gain, average daily weight gain, specific growth rate, feed conversion ratio, survival rate, total biomass and health indices (p < 0.05). The highest WG (39.11±1.49 g), ADWG, (1.00±0.12 g), SGR (8.74±0.03 % d-1) and lowest FCR (0.96±0.20) observed with T3 (22 ppt salinity) treatment, which was significantly higher compared to other treatment groups (p < 0.05). Among the health indices, the highest hepatosomatic index and viscerosomatic index found with 22 ppt salinity treatment, which was also significantly higher than the other treatment groups (p < 0.05). No significant differences were found among the treatments in terms of survival rate (p > 0.05), but the maximum survival rate (98.89±0.0 %) in T3 (22 ppt) and T2 (5 ppt). The maximum level of crude proteins (19.99±1.4%) was found in the whole-body biochemical composition (% of wet weight) of Asian sea bass juveniles in T3 treatment group reared at 22 ppt salinity. The second-order polynomial regression showed that 20 ppt salinity is optimum for the best growth of Asian sea bass. Thus, our present work would provide valuable information to the fish farmers for culturing the sea bass as well as its management along the inland and coastline of Pakistan.

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Assessment of growth characteristics, the survival rate and body composition of Asian sea bass Lates calcarifer (Bloch, 1790) under different feeding rates in closed aquaculture system

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2020.11.056 ◽

2020 ◽

Author(s):

Habib Ul Hassan ◽

Qadeer Mohammad Ali ◽

Naveed Ahmad ◽

Zubia Masood ◽

Md. Yeamin Hossain ◽

...

Keyword(s):

Body Composition ◽

Survival Rate ◽

Sea Bass ◽

Growth Characteristics ◽

Feeding Rates ◽

Lates Calcarifer ◽

Aquaculture System ◽

Asian Sea Bass

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Growth trials for polyculture of hatchery-reared juvenile spotted babylon (Babylonia areolata ) and sea bass (Lates calcarifer ) in a flow-through sea water system

Aquaculture Research ◽

10.1046/j.1365-2109.2001.00554.x ◽

2001 ◽

Vol 32 (3) ◽

pp. 247-250 ◽

Cited By ~ 3

Author(s):

N Chaitanawisuti ◽

A Kritsanapuntu ◽

S Kathinmai ◽

Y Natsukari

Keyword(s):

Sea Water ◽

Water System ◽

Sea Bass ◽

Lates Calcarifer ◽

Babylonia Areolata ◽

Flow Through

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The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

Aquatic Science and Technology ◽

10.5296/ast.v5i2.11594 ◽

2017 ◽

Vol 5 (2) ◽

pp. 1

Author(s):

Mulyati Mulyati ◽

Suryati Suryati ◽

Irfani Baga

Keyword(s):

Survival Rate ◽

Sea Water ◽

Challenge Test ◽

Probiotic Bacteria ◽

Pathogenicity Test ◽

Inhibitory Ability ◽

Significant Difference ◽

Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

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