scholarly journals Optimization of HIB Value Combination of Tween 60 and Span 80 on Cream Formulation of Ethanol Extract of Green Tea Leaves (Camellia Sinensis L.)

2018 ◽  
Vol 23 (3) ◽  
pp. 124
Author(s):  
Fransiska Lisa Anindya Putri ◽  
Akhmad Kharis Nugroho ◽  
Erna Prawita Setyowati
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Lattice Design ◽  
Dpph Method ◽  
Green Tea Leaves ◽  
Span 80 ◽  
Tween 60

Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.

scholarly journals EVALUASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 119-128
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Strong Activity ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves

Abstract Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

scholarly journals UJI AKTIVITAS SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 129-134
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Strong Activity ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves

Abstract   Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

scholarly journals Peel Off Gel Mask containing Green Tea Leaf Extract (Camellia sinesis L) with Antioxidant Activity

2021 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Anis Yohana Chaerunisaa ◽  
Emma Surachman ◽  
Zila Khuzaimah
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ph Value ◽  
Tea Leaves ◽  
Drying Time ◽  
Dpph Method ◽  
Ic50 Values ◽  
Green Tea Leaves ◽  
The Stability

Peel off mask has been very popular and preferred due to its ease in application. Gel base has been preferred for its cold and moist sensation during application. When herbal ingredients such as extract is  introduced into gel formulation, the stability then will become an issue. The aim of the study was to formulate the peel-off mask gels containing green tea leaves extract (Camellia sinensis L.) which effective, stable, and safe to be used. The antioxidant study was conducted by using  Diphenylhydrazylpicryl (DPPH) method. The peel-off mask gel formula were Hidroxypropyl Methylcelulosa (HPMC) as gel base at 0.5% concentration and plasticizer Polyvinyl Alcohol (PVA) at concentration of 12%. Evaluation of peel-off mask gel included observation on pH, viscosity, dispersing power, and drying time during the 90 days of storage at room temperature. The IC50 values of the green tea (Camellia sinensis L.) extract was 5.511 ppm. The concentration of green tea leaves extract in the preparation were 1x IC50 (F1); 2x IC50 (F2)  and 3x IC50 (F3). The result showed that all preparations were stable in the aspect of consistency, color, and odor. Viscosity, drying time and dispersing power remained unchanged after 90 days of storage. The pH value changed, but still in the range of acceptable pH of topical preparations. The results of antioxidant activity study showed that formula F3 (peel-off mask gels with 3 x IC50 of green tea extracts) had the highest antioxidant activity.Keywords: antioxidant, Camellia sinensis L., peel off gel masks, green tea

scholarly journals FORMULASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 109-118
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves ◽  
Tea Leaf

Abstract   Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

Enhancement of polyphenolic content extraction rate with maximal antioxidant activity from green tea leaves by cold plasma

2020 ◽  
Vol 85 (10) ◽  
pp. 3415-3422
Author(s):  
Maryam Keshavarzi ◽  
Gholamhassan Najafi ◽  
Hassan Ahmadi Gavlighi ◽  
Pourya Seyfi ◽  
Hamid Ghomi
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Cold Plasma ◽  
Extraction Rate ◽  
Tea Leaves ◽  
Content Extraction ◽  
Polyphenolic Content ◽  
Green Tea Leaves

scholarly journals Constituents of the Green Tea Seeds of Camellia sinensis

2011 ◽  
Vol 6 (3) ◽  
pp. 1934578X1100600
Author(s):  
Toshio Hasegawa ◽  
Kensuke Akutsu ◽  
Yasuhiro Kishi ◽  
Kouji Nakamura
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Nmr Spectroscopy ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Tea Leaves ◽  
Hexane Extracts ◽  
Green Tea Leaves

Green tea ( Camellia sinensis) leaves are known to contain active ingredients such as catechins and caffeine, and are widely useful materials. Recently, green tea flowers also have been in the spotlight. However, little attention has been paid to the tea seeds. In this work, the constituents of green tea seeds and green tea leaves were compared. Caffeine was found in the seeds, whereas catechins (usually obtained from green tea leaves) were not observed. Next, we investigated the constituents of hexane extracts and methanol extracts of green tea seeds. We found that the hexane extracts contained high amounts of oleic glyceride (79.9%) in addition to linoleic glyceride (20%). We confirmed the structures of these glycerides by NMR spectroscopy and by synthesis from a fatty acid and glycerol. The methanol extract was found to contain naringenin glucosides by mass spectrometry and NMR spectroscopic analysis.

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