scholarly journals IN VITRO ANTI-INFLAMMATORY AND CYTOTOXICITY OF CRINUM X AMABILE GROWN IN ECUADOR

2018 ◽  
Vol 11 (10) ◽  
pp. 99
Author(s):  
Vinueza Diego ◽  
Portero Santiago ◽  
Pilco Gisela ◽  
GarcÍa Marlene ◽  
Acosta Karen ◽  
...  
Keyword(s):  
Total Alkaloid ◽  
Inflammatory Activity ◽  
Tetrazolium Salt ◽  
Phytochemical Analysis ◽  
Total Alkaloids ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Alkaloid Extracts ◽  
Phytochemical Studies

Objective: The aim of this research was to assess the in vitro anti-inflammatory activity and cytotoxicity of the methanolic extract and total alkaloid extract obtained from leaves and bulbs of Crinum x amabile (CA) growing in Ecuador.Methods: Methanolic extracts of dry powered leaves and bulbs of CA obtained by cold maceration method were subjected to preliminary phytochemical screening. Total alkaloid extracts of leaves and bulbs of CA were obtained by conventional extraction of alkaloids base with an organic solvent. Furthermore, the anti-inflammatory activity and cytotoxicity of the four extracts were investigated by in vitro isolated neutrophils model using stable tetrazolium salt (WST-1).Results: Phytochemical analysis of methanolic extracts revealed the major classes of phytochemicals such as alkaloids, flavonoids, tannins, triterpenes, and steroids, but no proteins or saponins could be detected on leaves extract. Extracts obtained from bulbs both methanolic and total alkaloids of CA show an interesting anti-inflammatory activity, although it was not significant compared to the standard anti-inflammatory drug, aspirin. Cytotoxicity of bulb alkaloid extract was lower compared with all of the other extracts.Conclusion: Based on the results of this research, it could be concluded that CA is a very interesting source of natural anti-inflammatory compounds (especially alkaloids) which could be used to prevent many chronic disorders. Further, phytochemical studies are necessary to identify the chemical compounds responsible for the significant anti-inflammatory activity showed. 

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

scholarly journals Screenings of In-vitro antimicrobial, cytotoxic and anti-inflammatory activity of crude methanolic extracts of Crinum latifolium (Leaves)

2016 ◽  
Vol 10 (37) ◽  
pp. 649-655 ◽  
Author(s):  
Abdur Rahman Md. ◽  
Saddam Hussain Md. ◽  
Shalahuddin Millat Md. ◽  
Chandra Ray Mithun ◽  
Tohidul Amin Mohammad ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Methanolic Extracts ◽  
Anti Inflammatory

scholarly journals Natural populations of Galphimia spp. attenuates peripheral and central inflammation

2021 ◽  
Author(s):  
Reinier Gesto-Borroto ◽  
Gabriela Meneses ◽  
Alejandro Espinosa-Cerón ◽  
Guillermo Granados ◽  
Jacquelynne Cervantes-Torres ◽  
...  
Keyword(s):  
Systemic Inflammation ◽  
Natural Populations ◽  
Inflammatory Activity ◽  
Medicinal Species ◽  
Methanolic Extracts ◽  
Nitrite Production ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract The genus Galphimia is widely distributed in Mexico, and is represented by 22 species, including medicinal species. The sedative and anti-inflammatory effects of galphimines produced by the species Galphimia glauca have been documented. Formerly, molecular studies using DNA barcodes demonstrated that nine populations botanically classified as Galphimia glauca belong to four different species of the genus Galphimia, and that only one exhibited the sedative properties; however, all the collected species showed anti-inflammatory activity. Other bioactive compounds like quercetin, galphins, galphimidins and glaucacetalins have been identified from methanolic extracts of plants botanically classified as Galphimia glauca. The aim of this work was to determine the anti-inflammatory activity of methanolic extracts of nine collected Galphimia spp. populations grown in Mexico. The possible modes of action were analyzed by evaluating the inhibition of LPS-induced inflammation processes both in vitro and in vivo. The nine populations were evaluated by an in vitro model using RAW 264.7 murine macrophage cells, and two populations (a galphimine-producing and a non-galphimine-producing population) were selected for the in vivo experiments of systemic inflammation and neuroinflammation in mice. Results suggest that an anti-inflammatory in vitro effect was present in all the studied populations, evidenced by the inhibition of nitrite production. An inhibitory systemic inflammation in mice was exerted by the two analyzed populations. In the neuroinflammation model, the anti-inflammatory effect was demonstrated in methanolic extract of the non-galphimine-producing population. For the populations of Galphimia spp. studied herein, the anti-inflammatory effect could not be correlated to the presence of galphimines.

Phytochemical, In vitro Anti-inflammatory and Antimicrobial Potential of Hugonia mystax L.

2021 ◽  
pp. 169-173
Author(s):  
K.P. Jaiganesh ◽  
T.J. Jasna ◽  
A.C. Tangavelou
Keyword(s):  
Tamil Nadu ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Traditional System ◽  
Antimicrobial Potential ◽  
Biochemical Compounds ◽  
Anti Inflammatory

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.

scholarly journals Phytochemical analysis, antioxidant and anti-inflammatory activities of Chassalia kolly leaves extract, a plant used in Benin to treat skin illness

2021 ◽  
Vol 15 (3) ◽  
pp. 063-072
Author(s):  
Koudoro Yaya Alain ◽  
Awadji Jospin Morand ◽  
Botezatu Dediu Andreea ◽  
Olaye Théophile ◽  
Agbangnan Dossa Cokou Pascal ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Total Flavonoids ◽  
Skin Infections ◽  
Total Phenols ◽  
Anti Inflammatory

In the last decades, it has been intensively studied natural alternatives to synthetic products. Chassalia kolly is one of the medicinal plants used traditionally for treatment of skin infections. This study aimed at identifying the diversity secondary metabolites, to investigate the antioxidant and anti-inflammatory activities of ethanolic extract of Chassalia kolly leaves. Total phenols were determined by Folin Ciocalteu method. The aluminum trichloride method has been used to quantify total flavonoids, while the determination of condensed tannins was carried out by the hydrochloric vanillin method. The antioxidant capacity was evaluated by 2, 2-diphenyl-l-picrylhydrazyl (DPPH), 2, 2'-Azino-bis- (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and phosphomolybdenum methods. In vitro anti-inflammatory activity of ethanolic extract of Chassalia kolly leaves was evaluated by method membrane stabilization. In this results, Chassalia kolly leaves contain flavonoid, mucilages, anthocyans, sterols and terpenes, saponosides, alkaloids and reducing compounds. The contents of total phenols, total flavonoids and total tannins are respectively (20.41±2.11) µgGAE/mgDM; (30.29±2.18) µgQE/mgDM and (1.211±0.122)µgCE/mgDM. The results obtained during this work allowed us to assert that all extracts of the studied plant have very good antioxidant properties (IC50=0.05µg/µL). The ethanolic extract of the leaves of Chassalia kolly showed an anti-inflammatory activity more interesting than aspirin which is the reference compound used in this study.

scholarly journals Screening of Chloroform and Methanolic Bark Extracts of Commiphora caudata for its In-vitro Anti-bacterial, Anti-fungal and In-vivo Anti-inflammatory Activity

2021 ◽  
Vol 12 (4) ◽  
pp. 853-857
Author(s):  
Raju H V ◽  
Kishori P Sutar ◽  
Prasanna S Sutar ◽  
Shailendra S Suryawanshi ◽  
Nisha S Shirkoli
Keyword(s):  
Research Work ◽  
Extraction Procedure ◽  
Herbal Medicines ◽  
Developed Countries ◽  
Inflammatory Activity ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Bacteria And Fungi

Herbal medicines and their preparations have been widely used from the thousands of years in developing and developed countries in the primary health care of society and community. They have great demand due its safety, efficacy with minimum side or adverse effects. Commiphora caudata. It’s known as konda mava in kannada, ikkata in Sanskrit and hill mango in English. It’s widely used in the management of various disorders. Hence the identification of bioactive fractions from various parts of selected medicinal plant is important. In the present research work an attempt has been made to screen and assess the antibacterial, antifungal and anti-inflammatory activities of Commiphora caudata barks. The barks of selected plant material were collected, authenticated, powdered and subjected for extraction procedure. The extracts were screened for presence of various phytoconstituents. The antibacterial activity of chloroform and methanolic extracts were performed against various strains of bacteria and fungi. The extracts also were investigated for its in-vivo anti-inflammatory activity. The result of investigation concludes that chloroform and methanolic extract of plant were potential to inhibit the growth of selected strains of microorganism and also produced potential anti-inflammatory effect.

scholarly journals PHYTOCHEMICAL ANALYSIS AND SUPPRESSION OF INFLAMMATORY TARGETS BY ADATHODA VASICA

2018 ◽  
Vol 11 (5) ◽  
pp. 162
Author(s):  
Amala Reddy ◽  
Sujatha Sundaresan
Keyword(s):  
Methanolic Extract ◽  
Maximum Yield ◽  
Foot Ulcer ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Anti Inflammatory

 Objectives: A persistent and chronic inflammation may act as an underlying definition for many of the long-term illness such as diabetic foot ulcer, Alzheimer’s disease and rheumatoid arthritis. Folklore medicinal plants provide immense health benefits with new remedies. Active compounds from plants containing alkaloids and phenolic compounds (flavonoids and tannins) possess antioxidant and anti-inflammatory activity. The leaves of Adhatoda vasica are most known for its effectiveness in treating respiratory diseases such as asthma, chronic bronchitis, and other respiratory conditions. In this report, we investigated the possible action of A. vasica against inflammation.Methods: Solvents with varying polarity (Hexane, dichloromethane, ethyl acetate, and methanol) were used for the sequential extraction of leaves of A. vasica Linn (Family: Acanthaceae). The anti‐inflammatory activity was assessed using simple in vitro methods. Phenols, flavonoids, phytosterols, saponins, alkaloids, and tannins were analyzed qualitatively using standard protocols. Anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity. Aspirin (diclofenac) was used as a standard drug for the study of anti-inflammatory activity.Results: Our results substantiated that methanolic extract of A. vasica gave a maximum yield of 12.5% and apparently all bioactive phytochemical constituents were at a higher concentration in the methanolic extract. Methanolic extract of A. vasica showed a significant inhibition of the heat-induced albumin denaturation, red blood cells membrane stabilization, and proteinase inhibitory effects with 86±1.46, 88±1.24, and 96±0.93% for 1000 μg/ml, respectively.Conclusion: The above results delineate that the bioactive components were extracted using high polar solvent are mostly flavonoids and related polyphenols. These compounds may be the active constituents to render the anti-inflammatory activity to A. vasica.

scholarly journals Intestinal Anti-Inflammatory Activity ofBaccharis dracunculifoliain the Trinitrobenzenesulphonic Acid Model of Rat Colitis

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Sílvia Helena Cestari ◽  
Jairo Kennup Bastos ◽  
Luiz Claudio Di Stasi
Keyword(s):  
Lipid Peroxidation ◽  
Plant Extract ◽  
Inflammatory Activity ◽  
Glutathione Depletion ◽  
Phytochemical Analysis ◽  
Coumaric Acid ◽  
Brain Membrane ◽  
Anti Inflammatory ◽  
Improve Health

Baccharis dracunculifoliaDC (Asteraceae) is a Brazilian medicinal plant popularly used for its antiulcer and anti-inflammatory properties. This plant is the main botanical source of Brazilian green propolis, a natural product incorporated into food and beverages to improve health. The present study aimed to investigate the chemical profile and intestinal anti-inflammatory activity ofB. dracunculifoliaextract on experimental ulcerative colitis induced by trinitrobenzenosulfonic acid (TNBS). Colonic damage was evaluated macroscopically and biochemically through its evaluation of glutathione content and its myeloperoxidase (MPO) and alkaline phosphatase activities. Additionalin vitroexperiments were performed in order to test the antioxidant activity by inhibition of induced lipid peroxidation in the rat brain membrane. Phytochemical analysis was performed by HPLC using authentic standards. The administration of plant extract (5 and 50 mg kg−1) significantly attenuated the colonic damage induced by TNBS as evidenced both macroscopically and biochemically. This beneficial effect can be associated with an improvement in the colonic oxidative status, since plant extract prevented glutathione depletion, inhibited lipid peroxidation and reduced MPO activity. Caffeic acid,p-coumaric acid, aromadendrin-4-O-methyl ether, 3-prenyl-p-coumaric acid, 3,5-diprenyl-p-coumaric acid and baccharin were detected in the plant extract.

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