scholarly journals FLOW INJECTION SPECTROPHOTOMETRIC DETERMINATION OF NARINGENIN IN SUPPLEMENTS USING SOLID-PHASE REACTOR CONTAINING IMMOBILIZED MANGANESE DIOXIDE

2018 ◽  
Vol 11 (8) ◽  
pp. 473
Author(s):  
Israa M Jawad Al Mashhadani ◽  
Sadeem Subhi Abed
Keyword(s):  
Manganese Dioxide ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Coupling Reaction ◽  
Accurate Method ◽  
Oxidative Coupling Reaction ◽  
Daily Diet ◽  
Solid Phase Reactor ◽  
Colored Product

Objective: Naringenin (NAR) is a part of the human daily diet, and it plays an important role in human health for its biological functions. This study describes a new, sensitive, simple, and accurate method for determining NAR in supplements.Methods: The method is based on oxidative coupling reaction between NAR and N, N-dimethyl-p-phenylenediamine in an alkaline medium using manganese dioxide immobilized in cellulose acetate as online oxidant agent to form a colored product which can be monitored at λ max598 nm. Results: Several operating parameters such as reactor column length, particles size, chemicals, and physicals reaction conditions were studied. The proposed method was sensitive and good repeatable, the linear range of NAR concentration was from 1 to 70 μg/ml with a limit of detection of 0.292 μg/ml, and recovery range of analysis was 99.55–100.48%. Conclusion: The proposed method was successfully applied for determining NAR in supplements.

scholarly journals Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide

2020 ◽  
Vol 17 (3) ◽  
pp. 0780
Author(s):  
Takleef Sallal ◽  
Sumayha Abbas
Keyword(s):  
Limit Of Detection ◽  
Coupling Reaction ◽  
Accurate Method ◽  
Molar Absorptivity ◽  
Mean Value ◽  
Oxidative Coupling Reaction ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Colored Product ◽  
Hydrolysis Of

Rapid, reproducible and accurate method has been developed for the assay for of mebendazol (MBZ) residual assay. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide then oxidation with N-bromosuccinimide (NBS) followed by coupling with 4-Bromoaniline (4-BA) to yield a highly colored product absorbed at maximum 434 nm. Regression analysis of linearity range was found (0.6-2.8) µg.ml-1.  The optimum conditions that affect the oxidation were studied. The developed method was found to be precise with mean value of relative standard deviation (1.153- 1.303) and accurate with relative error (-0.5940-1.7821) .The calculated molar absorptivity and sandal sensitivity values of (29825 L.mol-1.cm-1), 0.0099 µg.cm-2 respectively.  The limit of detection and limit of quantitation were of 0.04696, 0.156548 µg.ml-1 respectively .The suggested method showed good recovery with a mean value of 100.77% for analysis of dosage forms.

Determination of Bendiocarb Insecticide by Reverse Flow Injection Analysis Using a Solid-Phase Reactor Containing Micro PbO2, Nano PbO2 and Grafted SiO2 - PbO2 Immobilized

2020 ◽  
Vol 42 (1) ◽  
pp. 31-31
Author(s):  
Malik H Alaloosh Alamri Malik H Alaloosh Alamri ◽  
Sadeem Subhi Abed and Abdulkareem M A Alsammarraie Sadeem Subhi Abed and Abdulkareem M A Alsammarraie
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Reverse Flow ◽  
Blue Color ◽  
Wide Range ◽  
Solid Phase Reactor ◽  
Grafted Nanoparticles ◽  
Reverse Flow Injection

Bendiocarb (BEN) is an acutely toxic carbamate insecticide which used in public places and agriculture, it is also effective against a wide range of nuisance and disease vector insects. A new rapid and sensitive reverse flow injection spectrophotometric procedure coupled with on-line solid-phase reactor is designed in this article for the determination of BEN in its insecticidal formulations and water samples, by using three different solid-phase reactors containing bulk PbO2 (B-SPR), PbO2 nanoparticles (N-SPR) and grafted nanoparticles of SiO2-PbO2 (G-SPR) immobilized on cellulose acetate matrix (CA). This method of oxidative coupling is based on alkaline hydrolysis of the BEN pesticide, and then coupled with N,N dimethyl-p-phenylenediamine sulphate (DMPD) to give a blue color product which measured at λmax 675 nm. It worth to mentioned that under optimal conditions, Beer’s law is obeyed in the range of 1-175 μg mL-1 for B-SPR and 0.25-70 μg mL-1 of BEN for both N-SPR and G-SPR respectively within limit of detection (LOD) of 0.931, 0.234 and 0.210 μg mL-1 for B-SPR N-SPR and G-SPR respectively. The surface methodology of the solid phase was also investigated by using atomic force microscopy.

scholarly journals Rapid and Sensitive Spectrophotometric Method for the Determination of the Trace Amount of Thallium (III) in Water and Urine Samples by New Oxidative Coupling Reaction

2019 ◽  
Vol 53 (4) ◽  
Author(s):  
Padmarajaiah Nagaraja ◽  
Naef Ghllab Saeed Al-Tayar ◽  
Anantharaman Shivakumar ◽  
Ashwinee Kumar Shresta ◽  
Avinash K. Gowda
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Urine Samples ◽  
Oxidative Coupling Reaction ◽  
Relative Standard ◽  
Student’S T

A very simple, sensitive and fairly selective direct spectrophotometric method is presented for the rapid determination of thallium(III) at trace level. The method is based on the oxidation of 2-hydrazono-3-methyl-2,3-dihydrobenzo[d]thiazole hydrochloride (MBTH) by thallium(III) in phosphoric acid medium to form a diazoniumcation, which couples immediately with 10,11-dihydro-5Hdibenzo[b,f]azepine (IDB) at room temperature giving a blue colored species having a maximum absorption at 660 nm. The reaction conditions and other important analytical parameters were optimized.The calibration curve was found to be linear over the range of 0.1-4 μg/mL with molar absorptivity of 4.5 × 104 L mol- cm-1 and Sandell’s sensitivity of 0.00454 μg cm-2. The relative standard deviation and limit of detection have been found to be 0.58% and 0.0147 μg/mL respectively. Almost all common anions and cations are found notto interfering in matrix level of the analytical process. The method has been successfully applied for the determination of thallium(III) in synthetic standard mixtures, water and human urine samples. The performance of proposed method was evaluated in terms of student’s t-test and variance ratio F-test, to find out the significance of proposed method over the reported methods.    

scholarly journals Spectrophotometric Determination of Azithromycin using Oxidative Coupling Reaction

2020 ◽  
Vol 10 (03) ◽  
pp. 389-394
Author(s):  
Maadh T. Abdulrahman ◽  
Ihab M. Rashid ◽  
Nashwan H. Ali
Keyword(s):  
Oxidative Coupling ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Sensitivity Index ◽  
Oxidative Coupling Reaction ◽  
Relative Standard ◽  
Sensitivity And Selectivity ◽  
Colored Product ◽  
The Stability

For determining the azithromycin (AZT) in medicinal and pure formulas, a simple spectrophotometric technique was developed. An approach suggested is dependent on an AZT’s oxidative coupling reaction by sodium period (SPI) and 4-amino antipyrine (AAP) producing a pink colored compound with optimum absorption of 480 nm. Different experimental parameters are extensively researched and mastered, which affects the stability of a colored product formed, then developed. The law of the Beer is obeyed over its concentration range 3 to 44 ppm, whereas the limit of detection and quantification is 0.1908 and 0.5726 ppm, respectively, for a connection factor (r) = 0.9998. Also calculated are the molar absorption of 8.23 × 103 L/mol.cm, and the sensitivity index for Sandell is 7 × 10-5 mg/cm2. A method’s accuracy and precision are tested by also determining a relative standard deviation (RSD) less than 0.645 percent, and 100.189 percent average recovery. Practically possible external interferences about a calculation for AZT are checked in drug tablets. The results demonstrated that the procedure for determining AZT was successful in its application in pharmaceutical preparations. Comparing the literature survey that shows good sensitivity and selectivity, the reliability of the proposed method is chalked in.

Simultaneous Spectrophotometric Determination of Salbutamol by Coupling with Diazotized 4-Aminobenzoic acid

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Hind Hadi ◽  
Gufran Salim
Keyword(s):  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Dosage Forms ◽  
Water Soluble ◽  
Trace Determination ◽  
Aminobenzoic Acid ◽  
Optimum Conditions ◽  
Colored Product ◽  
Versus Concentration

A simple, rapid and sensitive spectrophotmetric method for trace determination of salbutamol (SAL) in aqueous solution and in pharmaceutical preparations is described. The method is based on the diazotization coupling reaction of the intended compound with 4-amino benzoic acid (ABA) in alkaline medium to form an intense orange, water soluble dye that is stable and shows maximum absorption at 410 nm. A graph of absorbance versus concentration indicates that Beer’s law is obeyed over the concentration range of 0.5-30 ppm, with a molar absorbtivity 3.76×104 L.mol-1 .cm-1 depending on the concentration of SAL. The optimum conditions and stability of the colored product have been investigated and the method was applied successfully to the determination of SAL in dosage forms.

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