EVALUATION OF ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF MEDICINAL PLANTS

2020 ◽  
pp. 182-185
Author(s):  
KARTHIKA S ◽  
KANNAPPAN N ◽  
SURIYAPRAKASH TNK
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Lipid Peroxide ◽  
Cat Assay ◽  
Peristrophe Bicalyculata ◽  
In Vitro Antioxidant Activity

Objective: The present study was carried out to evaluate the antioxidant activity of different extracts of two medicinal plants. Methods: In-vitro antioxidant activity of different extracts of Hydrocotyle javanica Thunb and Peristrophe bicalyculata (Retz.) Nees were determined by following methods such as Lipid peroxide assay (LPO), nitric oxide assay (NO), glutathione (GSH), catalase (CAT). Results: Among eight different extracts, n-Hexane H. javanica extract and alcoholic P. bicalyculata extract showed more potent in vitro antioxidant activity in terms of LPO, NO, GSH, SOD, and CAT assay. Conclusion: From the results, it can be concluded that n-Hexane H. javanica extract and alcoholic P. bicalyculata extract showed more potent in vitro antioxidant activity.

In vitro antioxidant activity and phenolic contents in methanol extracts from medicinal plants

2012 ◽  
Vol 22 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Sanjay Guleria ◽  
A. K. Tiku ◽  
Gurjinder Singh ◽  
Apurva Koul ◽  
Sahil Gupta ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Methanol Extracts ◽  
Phenolic Contents ◽  
In Vitro Antioxidant Activity

scholarly journals Invitro Efficacy of antioxidant activity in ethanolic and aqueous leaf extracts of Andrographis paniculata Nees and Rhinacanthus nasutus Kurz

2020 ◽  
Vol 11 (4) ◽  
pp. 6301-6306
Author(s):  
Akilandeswari G ◽  
Bupesh G ◽  
Vijaya Anand A ◽  
Saradhadevi K M ◽  
Mayur Mausoom Phukan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Radical Scavenging ◽  
The Body ◽  
Andrographis Paniculata ◽  
Peroxide Radical ◽  
Leaf Extracts ◽  
Rhinacanthus Nasutus ◽  
In Vitro Antioxidant Activity

Plants are adundantly and are very promisive to be used as source of drugs in many diseases or infections and also it is a main agents of antioxidants which prevents the oxidative stress that are caused by the free radicals. There are numerous studies based on the pharmaceutical and classification of medicinal plants throughout the world. Leaves, fruits, roots are most frequently plant parts used in many research and studies. Here the present study was aimed to evaluate the in vitro antioxidant activity of aqueous and ethanol leaf extracts of Andrographis paniculata Nees and Rhinacanthus nasutus Kurz. Antioxidant is a substance which is used to prevent some types of cell damage in the body. Determination of their in vitro antioxidant activity were carried out by using methods such as DPPH (2,2-diphenyl-1picrylhydrazyl assay), ABTS (2,2′-azinobis (3-ethylbenzothiazolin 6-sulfonic acid assay), FRAP (ferric reducing antioxidant power assay) and SOD (super oxide anion scavenging) assay, H2O2 (hydrogen peroxide radical scavenging) assay. Moreover the ethanolic leaf extracts showed best antioxidant activity than the aqueous leaf extracts. Experimental results reveals that the leaves of A. paniculata have potent antioxidant and free radical scavenging activity than R. nasutus. Further investigation must be done for these two medicinal plants for the discovery of the bioactive compounds.

scholarly journals In Vitro antioxidant activity of Polygonum Glabrum 

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Raja Sundara rajan ◽  
Ramya I
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Superoxide Anion ◽  
Methanol Extract ◽  
Butylated Hydroxytoluene ◽  
In Vitro Antioxidant Activity

<p>Assessment of antioxidant activity was imperative in the screening of medicinal plants for potential health benefits. In present study methanol extract of <em>Polygonum glabrum</em> <em> </em>(polygonaceae) was screened for its in vitro antioxidant activity using biologically relevant methodologies which scavenge radicals such as  1,1 diphenyl 2 picryl hydrazyl (DPPH), nitric oxide, hydrogen peroxide, hydroxyl,  superoxide anion and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Total reducing ability by conversion of ferric (III) to ferrous (II) and molybdenum (VI) to molybdenum (V), metal ion chelating capacity and anti lipid peroxidation activities were also examined. The antioxidant ability of <em>Polygonum glabrum</em> whole plant extract was found to be in a dose dependent manner. The IC<sub>50</sub> values for scavenging of DPPH<sup>●</sup> and ABTS<sup>●+</sup> free radicals were 13.18 μg/ml and 20.46 μg/ml. For scavenging of nitric oxide, hydrogen peroxide, hydroxyl and superoxide anion radicals, the IC<sub>50</sub> values were found to be 80.22 μg/ml, 33.06 μg/ml, 52.26μg/ml and 36.98 μg/ml respectively. Further, addition of 120μg/ml of plant extract to the reaction mixture produced 50% lipid peroxidation inhibition activity. Commercial antioxidants such as vitamin E, quercetin, butylated hydroxytoluene and ascorbic acid were used as reference compounds. The strong antioxidant activity of <em>Polygonum glabrum</em> may be credited to the presence of triterpenes [beta-hydroxyfriedalanol], phenols        [3-hydroxy-5-methoxystilbene], flavonoids [pinocembrin and pinocembrin-5-methylether], steroids [sitosterol - (6-O-palmitoyl)- 3-O-β-D glucopyranoside and sitosterol-3-O-β-D glucopyranoside], sesqueterpenes [2,3-dihydroxy isodrimeninol] and pigments etc in methanol extract.</p>

Evaluation of in vitro antioxidant activity of selected Peruvian medicinal plants

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
B Svobodova ◽  
L Polesna ◽  
M Orsak ◽  
J Lachman ◽  
J Vadlejch ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
In Vitro Antioxidant Activity

scholarly journals Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
RICO RAMADHAN ◽  
ALFINDA NOVI KRISTANTI ◽  
RUDIANTO AMIRTA ◽  
IRAWAN WIJAYA KUSUMA ◽  
PREECHA PHUWAPRAISIRISAN ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Antidiabetic Activity ◽  
East Kalimantan ◽  
Ic50 Value

Abstract. Ramadhan R, Kristanti AN, Amirta R, Kusuma IW, Phuwapraisirisan P, Haqiqi MT, Saparwadi. 2019. Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia. Biodiversitas 20: 1820-1826. Ten selected plants in this study have been used traditionally in West Kutai, East Kalimantan as medicinal plants. This study aimed to screen and evaluate the in vitro antidiabetic and antioxidant activity of methanol extracts of selected plants from East Kalimantan. The antidiabetic activity was carried out by α-glucosidase inhibitory activity with maltose and sucrose as substrates while antioxidant activity was determined by free radicals scavenging activity against DPPH, ABTS and Nitric Oxide (NO). The results showed that Garcinia riedeliana extract had strong inhibitory activity with an IC50 value of 0.04 mg/mL followed by  Shorea laevis, Pternandra azurea and Bridelia tomentosa with IC50 values of 0.12; 0.15 and 0.16 mg/mL in maltose respectively, while quercetin as positive control had an IC50 value of 0.08 mg/mL. G.riedeliana extract also showed good α-glucosidase inhibitory activity with an IC50 value of 0.23 mg/mL in sucrose as substrate. The results on antioxidant activity showed that all extracts have IC50 value against free radicals DPPH, ABTS, and nitric oxide. The most active extract as α-glucosidase inhibitory activity and antioxidant activity was G. riedeliana extract. These findings provide the scientific evidence that one species of medicinal plants from East Kalimantan might be potential as an antioxidant and α-glucosidase inhibitor.                                 

Evaluation of in Vitro Antioxidant Activity of Two Indian Medicinal Plants

2020 ◽  
Author(s):  
Karthiyayini R ◽  
Dhanya Ponraj ◽  
Yanchen Dolma Kom ◽  
Amutha Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Indian Medicinal Plants ◽  
In Vitro Antioxidant Activity
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