EVALUATION OF ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF MEDICINAL PLANTS

Objective: The present study was carried out to evaluate the antioxidant activity of different extracts of two medicinal plants. Methods: In-vitro antioxidant activity of different extracts of Hydrocotyle javanica Thunb and Peristrophe bicalyculata (Retz.) Nees were determined by following methods such as Lipid peroxide assay (LPO), nitric oxide assay (NO), glutathione (GSH), catalase (CAT). Results: Among eight different extracts, n-Hexane H. javanica extract and alcoholic P. bicalyculata extract showed more potent in vitro antioxidant activity in terms of LPO, NO, GSH, SOD, and CAT assay. Conclusion: From the results, it can be concluded that n-Hexane H. javanica extract and alcoholic P. bicalyculata extract showed more potent in vitro antioxidant activity.

FORMULATION, DEVELOPMENT AND EVALUATION OF HERBAL TRANSDERMAL PATCH FOR FRACTURE HEALING

Peristrophe bicalyculata posses expectorant, analgesic, anti-inflamatory, antipyretic and antibacterial properties and has been traditionally used for the management of pain, sprain, and bone fracture. In this study we have tried to incorporate bioactive components of P. bicalyculata in transdermal patches which were developed by solvent casting method. Formulated patches were further evaluated for thickness, folding endurance, moisture content and moisture reuptake. Formulations showing good folding endurance were selected for estimation of the drug content and in-vitro drug release. The patches showing drug content more than 90% and drug release 85% were selected and fitted for Zero-order kinetics. This study will help in the development of transdermal patch for the treatment of bone fracture.

ANTIOXIDANT, PHYTOCHEMICAL AND ANTIBACTERIAL ACTION OF HIMALAYAN MEDICINAL HERBS PERISTROPHE BICALYCULATA LEAVES EXTRACT AGAINST RESPIRATORY TRACT PATHOGENS

Objective: Evaluate the antioxidant, phytochemicals and antibacterial screening of leaves extracts of Peristrophe bicalyculata.Methods: The antibacterial activity against the respiratory tract pathogens i.e., Staphylococcus aureus (MTCC 1144), Streptococcus pneumoniae (MTCC 655), Streptococcus pyogenes (MTCC 442), Pseudomonas aeruginosa (MTCC 2474) and Klebsiella pneumoniae (MTCC 4030) was examined by the method agar well diffusion and the minimum inhibitory concentration (MICs) was determined by the method of twofold serial dilution. Broad spectrum antibiotic erythromycin was used as positive control and dimethyl sulphoxide (DMSO) used as negative control. The qualitative method was adapted for phytoconstituents screening and antioxidant activity of plant extract was examined by DPPH free radical scavenging method.Results: The results showed that the chloroform (CHF) extract has a higher degree of antibacterial potency then the other extract. The zone of inhibition showed by chloroform extract against tested bacteria ranged between 9.3±0.59 mm to 26.6±0.66 mm, respectively. MICs values were recorded between 6.25 mg/ml to 25 mg/ml against all the test organisms. Phytoconstituents analysis of P. bicalyculata extract exposed the presence of alkaloids, flavonoids, glycosides, steroids, saponins and tannins. The methanolic extract of P. bicalyculata % inhibition of DPPH radical is up to 86.33%. The P. bicalyculata (Methanolic extract) gives best antioxidant activity than another extract.Conclusion: This investigation ropes a good answer to the use of P. bicalyculata as a natural antioxidant and in herbal medicine as a support for the development of new drugs and phytomedicine in the foundation for its use in remedial of respiratory infectious diseases.

INDUCTION OF CALLOGENESIS AND SHOOT REGENERATION OF A MEDICINAL PLANT SPECIES PERISTROPHE BICALYCULATA (RETZ.) NEES.

In the present study, protocol for callus induction and regeneration for the medicinal plant species, Peristrophe bicalyculata (Retz.)Nees has been developed by using leaf explants. Young apical leaf explant was used for callus induction on MS medium containing BAP and NAA at 2`0 and 0.8 mgl-1 respectively showedmaximum callus induction (80%). The amount of callus responded for shoot formation (81%) was obtained in the MS medium containing BAP (2.0 mgl-1) and GA3 (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (2.0 mgl-1) and IAA (0.2 mgl-1) for shoots rooted. Regeneratedplantlet were successfully acclimatized and hardened off inside the culture and then transferred to green Peristrophe bicalyculata, MS medium, multiple shooting, Acclimatizationhouse with better survival rate