scholarly journals Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
RICO RAMADHAN ◽  
ALFINDA NOVI KRISTANTI ◽  
RUDIANTO AMIRTA ◽  
IRAWAN WIJAYA KUSUMA ◽  
PREECHA PHUWAPRAISIRISAN ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Antidiabetic Activity ◽  
East Kalimantan ◽  
Ic50 Value

Abstract. Ramadhan R, Kristanti AN, Amirta R, Kusuma IW, Phuwapraisirisan P, Haqiqi MT, Saparwadi. 2019. Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia. Biodiversitas 20: 1820-1826. Ten selected plants in this study have been used traditionally in West Kutai, East Kalimantan as medicinal plants. This study aimed to screen and evaluate the in vitro antidiabetic and antioxidant activity of methanol extracts of selected plants from East Kalimantan. The antidiabetic activity was carried out by α-glucosidase inhibitory activity with maltose and sucrose as substrates while antioxidant activity was determined by free radicals scavenging activity against DPPH, ABTS and Nitric Oxide (NO). The results showed that Garcinia riedeliana extract had strong inhibitory activity with an IC50 value of 0.04 mg/mL followed by  Shorea laevis, Pternandra azurea and Bridelia tomentosa with IC50 values of 0.12; 0.15 and 0.16 mg/mL in maltose respectively, while quercetin as positive control had an IC50 value of 0.08 mg/mL. G.riedeliana extract also showed good α-glucosidase inhibitory activity with an IC50 value of 0.23 mg/mL in sucrose as substrate. The results on antioxidant activity showed that all extracts have IC50 value against free radicals DPPH, ABTS, and nitric oxide. The most active extract as α-glucosidase inhibitory activity and antioxidant activity was G. riedeliana extract. These findings provide the scientific evidence that one species of medicinal plants from East Kalimantan might be potential as an antioxidant and α-glucosidase inhibitor.                                 

scholarly journals INVESTIGATION OF ANTIOXIDANT POTENTIAL OF QUERCETIN AND HESPERIDIN: AN IN VITRO APPROACH

2017 ◽  
Vol 10 (11) ◽  
pp. 83 ◽  
Author(s):  
Srimathi Priyanga K ◽  
Vijayalakshmi K
Keyword(s):  
Nitric Oxide ◽  
Free Radicals ◽  
High Efficiency ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Peroxide Radical ◽  
Ic50 Value ◽  
Reducing Capacity

  Objective: The study was carried out to evaluate the antioxidant potential of quercetin and hesperidin.Methods: The antioxidant potential of quercetin and hesperidin was estimated using free radicals in in vitro condition. Antioxidant activity of quercetin and hesperidin was estimated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, nitric oxide, hydroxyl radical, hydrogen peroxide radical, and reducing power assay.Results: Quercetin and hesperidin have high efficiency in scavenging free radicals. Both the compounds are more efficient in scavenging DPPH (IC50 value 36.15±0.30 and 41.55±0.49 mg/ml) and superoxide radicals (IC 50 value 19.3±0.26 and 28.08 ±0.18 mg/ml) have high reducing capacity when compared to the standards ascorbic acid (IC50 value 31.45±1.31 and 23.2±0.20 mg/ml).Conclusion: The present study suggests that quercetin and hesperidin have significant antioxidant activities and could be easily accessible to the immune system.

scholarly journals Antioxidant potential of some Sudanese medicinal plants used in traditional medicine

2015 ◽  
Vol 3 (2) ◽  
pp. 192 ◽  
Author(s):  
Hatil EL-Kamali ◽  
Sana E. M. Hamed
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
In Vitro Models ◽  
Water Extracts ◽  
Total Antioxidant ◽  
Sudanese Medicinal Plants

<p>In the present work methanolic and water extracts of 19 medicinal plants, commonly used in Sudanese folk medicines against gastrointestinal tract, infectious diseases were investigated for their antioxidant activity. Antioxidant assays were carried out by using different in vitro models such as DPPH (2, 2-diphenyl-1picrylhydrazyl) radical scavenging and Iron chelating activity. Methanolic and water extracts of <em>Punica granatum </em>showed the highest total antioxidant activity of 71±0.03 and 73±0.01 respectively. The antioxidant activities of water extracts were poor when compared to the methanol extract. The iron chelating power of methanol and aqueous extracts of <em>Commiphora myrrha</em> 58±0.01 and <em>Azadirachta indica</em> 57±0.20 respectively.</p>

scholarly journals Antioxidant Activity of Medicinal Plants from Habitats with Different Anthropogenic Loads

2020 ◽  
Vol 6 (2) ◽  
pp. 119-127
Author(s):  
S.A. Kurbatov ◽  
◽  
V.M. Zubkova ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Human Body ◽  
Raw Materials ◽  
Mechanism Of Formation ◽  
Analysis Of Results ◽  
Biochemical Research ◽  
First Time

the analysis of results of biochemical research concerning antioxidant activity of medicinal raw materials, with use of a method of inhibition of autooxidation of adrenaline in vitro is carried out. The mechanism of formation of free radicals and their sources is considered. A brief overview of the action of free radicals on the human body and cells is given. For the first time, depending on the biological characteristics, the antioxidant activity of plants in areas characterized by different anthropogenic loads was studied: an increase in the anthropogenic load was accompanied by a decrease in the antioxidant activity of plants.

scholarly journals In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Canthium coromandelicum Leaves

2020 ◽  
pp. 12-18
Author(s):  
S. Thiripura Salini ◽  
S. Shankar
Keyword(s):  
Diabetic Complications ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Antidiabetic Activity ◽  
Dpph Radical ◽  
Dpph Assay ◽  
Ic50 Value ◽  
Ic50 Values ◽  
In Vitro Antioxidant Activity

Oxidative stress plays a major role in diabetic complications. The study aim was to investigate the in-vitro antidiabetic and antioxidant activities of methanolic extract of Canthium coromandelicum leaves. The plant material was extracted with methanol and the methanolic extract was screened for in-vitro antioxidant activity using 1, 1-diphenyl-2-picryl hydrazyl (DPPH) assay. The efficiency of the antidiabetic activity of the plant extract was evaluated against α-amylase and α-glucosidase digestive enzymes. The study revealed that the C. coromandelicum extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value of 31.52 ± 0.42 and 41.49 ± 0.28 µg/mL respectively and compared with standard acarbose drug. The extract efficiently scavenging DPPH radical with IC50 values of 65.46 ± 0.50 µg/ml. Therefore, the extract could be a promising therapeutic in management of diabetic complications.

scholarly journals Evaluation of the antioxidant activity of various extracts of aerial parts of Cassia absus: An in-vitro techniques

2020 ◽  
Vol 11 (2) ◽  
pp. 1545-1550
Author(s):  
Mythri M ◽  
Sanal Dev K T ◽  
Kottai Muthu A
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Potential ◽  
In Vitro Techniques ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
The Difference

Cassia absus (Linn)Cassia absus(Linn) (family Fabaceae ) is generally known as “chaksu ” inan ayurvedic traditional system.The current study,aerial parts of different concentrates(Pet.ether, ethyl acetate and methanol) of Cassia absus, was evaluated for its in-vitro antioxidant potential byDiphenylpicrylhydrazyl radical,nitric oxide activity andtotal antioxidant activitytaking ascorbate as the standardfor all the three methods. The IC50 value was originated that methanolic concentrates of Cassia absusmore efficient inDiphenylpicrylhydrazyl radical,nitric oxide activity, total antioxidant activitycompared EA&PEconcentrates.The methanolic concentrates of Cassia absus& ascorbic acid exhibited antioxidant potential possessing IC50230µg/ml &130µg/ml (Nitric oxide). 205µg/ml &57µg/ml (total antioxidant),195µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical)respectively. The difference in the scavenging potential of the extracts can be due to variation in the percentage of bioactive compounds present in different solvents. Invitroantioxidant studiesobviouslyshow the methanolic concentrates of Cassia absushave better antioxidant activity. This result indicates that aerial parts of methanolic concentratesCassia absuscould serve as a natural antioxidant, which may be useful in preventfree radical-induced diseases.

In Vitro Acetylcholinesterase Inhibitory and Antioxidant Activity of Alphonsea tonkinensis A.DC

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110421
Author(s):  
Khan Viet Nguyen ◽  
Thu Oanh Thi Nguyen ◽  
Duc Viet Ho ◽  
Jyrki Heinämäki ◽  
Ain Raal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Phase ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Chromatographic Technique ◽  
Pure Compounds ◽  
Stems And Leaves

Knowledge of the bioactivity of Alphonsea tonkinensis A.DC is limited. We have investigated the in vitro acetylcholinesterase inhibitory and antioxidant activities of extracts and pure compounds isolated from stems and leaves of this species collected from Dakrong district, Quang Tri Province, Vietnam. Extracts and isolated compounds were obtained by using an in-house extraction and chromatographic technique. The in vitro acetylcholinesterase inhibitory and antioxidant activities were evaluated using an Ellman test and 2,2-diphenyl-1-picryl-hydrazyl test, respectively. The total MeOH and CH2Cl2 extracts, the MeOH portion of the CH2Cl2 extract, pseudocolumbamine, and pseudopalmatine showed potential inhibitory activity against acetylcholinesterase with IC50 values of 22.7, 32.9, 14.6, 18.9, and 8.6 μM, respectively. The aqueous phase (pH 9), MeOH portion of the CH2Cl2 extract, and N- trans-feruloyltyramin exhibited significant antioxidant activities with IC50 values of 24.5, 72.1, and 61.2 µM, respectively. This is the first study showing such bioactivities of various extracts obtained from A. tonkinensis.

Vitamin C in Acerola and Red Plum Extracts: Quantification via HPLC, in Vitro Antioxidant Activity, and Stability of their Gel and Emulsion Formulations

2018 ◽  
Vol 101 (5) ◽  
pp. 1461-1465 ◽  
Author(s):  
Letícia Caramori Cefali ◽  
Ludmila de Oliveira Maia ◽  
Rebeca Stahlschimidt ◽  
Janaína Artem Ataide ◽  
Elias Basile Tambourgi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Accelerated Aging ◽  
Stability Study ◽  
Transparent Glass ◽  
Glass Containers ◽  
In Vitro Antioxidant Activity

Abstract Background: The fruits acerola and red plum are known to be good sources of antioxidants, particularly vitamin C. Antioxidants are compounds that protect organisms from biomolecular damage, such as accelerated aging, caused by free radicals. Objective: The objective of this study was to extract vitamin C from acerola and red plum, incorporate these extracts into different topical formulations, and evaluate the physicochemical stabilities of these formulations under stress conditions. Methods: Vitamin C was extracted from acerola and red plum via dynamic maceration for 2 h at 50 ± 2°C and was quantified via HPLC. In vitro antioxidant activities were evaluated using DPPH assays. The extracts were then incorporated into emulsion and gel formulations in two types of packaging, and stability studies were carried out. Results: Red plum and acerola extracts were orange and red and contained vitamin C concentrations of 2732.70 ± 93.01 mg/100 g and 2.60 ± 1.2 mg/100 g, respectively. In vitro antioxidant activity resulted in over 90.0% inhibition of free radicals at 0.01 mL/mL acerola extract and 0.1 mL/mL red plum extract. In the stability study, pH values decreased for both acerola formulations when stored in the oven or in transparent glass containers. Formulations containing red plum extract were stable under all conditions. Acerola extracts contained a higher concentration of vitamin C than red plum extracts. Both extracts possessed antioxidant activity, although the acerola-based formulation was unstable when stored at high temperatures or in transparent glass containers. Highlights: Extracts from red plum and acerola contained vitamin C; antioxidant activity of the extracts resulted in over 90.0% inhibition of free radicals. Formulations containing red plum were stable under all tested conditions, and formulations containing acerola were unstable when stored in the oven or in transparent glass containers.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)

2016 ◽  
Vol 3 (2) ◽  
pp. 159-163
Author(s):  
Arif Rahman ◽  
Abd Malik ◽  
Aktsar Roskiana Ahmad
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Free Radical Scavengers ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dried Fruit ◽  
Ethanol Extracts

Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.

scholarly journals Phytochemical Investigation and In Vitro Antioxidant Activities of Tetracarpidium conophorum (African Walnut) Seeds

2018 ◽  
Vol 6 (2) ◽  
pp. 56-61
Author(s):  
Kelly Oriakhi ◽  
Kissinger Orumwensodia ◽  
Patrick Uadia
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Crude Methanol Extract

Background: Tetracarpidium conophorum (African walnut) is an African plant with ethnobotanical uses. Objectives: The purpose of this study was to evaluate the phytochemical screening and in vitro antioxidant activities of methanol extract and fractions (F) [n-hexane (HEX-F), dichloromethane (DCM-F) and, ethyl acetate (EA-F)] of T. conophorum seeds. Methods: Phytochemical screening and in vitro antioxidant activity study were carried out using DPPH, ABTs radical scavenging assays, nitric oxide inhibitory and reducing potential assays. Results: Methanol extract and its fractions contain phenols, flavonoids, saponins, tannins, terpenoids, and alkaloids. The concentrations of total phenols and flavonoids content were significantly higher in EA-F and crude methanol extract compared to other fractions. Crude methanol and EA-F contain higher concentrations of tannin while hexane fraction had the lowest tannin content but relatively higher proanthocyanidin content compared to other fractions. The antioxidant activity study showed that both methanol crude extract and fractions of T. conophorum seeds have significant activities for DPPH radical scavenging, reducing power, ferric reducing antioxidant potential, nitric oxide inhibitory activities, ABTS and hydroxyl radical scavenging for. DPPH radical scavenging activities of EA-F showed the lowest IC50 of 33.11 µg/mL, followed by Hex-F, DCM-F and crude methanol extract with IC50 of 33.43, 42.09 and 45.44 µg/mL, respectively, when compared to ascorbic acid with IC50 of 17.08 µg/mL. Conclusion: The study showed that T. conophorum seed is a rich source of secondary metabolites, which may be responsible for its antioxidant activities.

scholarly journals A DESCRIPTIVE STUDY AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF TRIDAX PROCUMBENS LINN

2021 ◽  
Vol 11 (6) ◽  
pp. 1-4
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ferric Chloride ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Alcoholic Extract ◽  
Tridax Procumbens

Tridax Procumbens Linn is a member of the Asteraceae family. Tridax Procumbens has to be utilized because native medication intended for a number of complaints and problems in humans and animals for thousands of years. It is used widely in Indian conventional remedies for healing of wounds, like anticoagulants, in fungal infection, in diarrhea and dysentery, as an antioxidant, antimicrobial, anti-inflammatory, and immunomodulators. In folk medicine, certain communicable skin diseases are treated by using leaves extract. It's also known as 'Bhringraj,' an ayurvedic drug used to treat liver problems. At least 12,000 people have been separated from their families. These compounds protect plants from pathogens, insects, and herbivores by acting as defensive mechanisms. The aim of the test is to establish the antioxidant potential of the leaves of Tridax Procumbens. The current research is aimed at identifying novel plant directions, and antioxidant activity has been chosen for that reason. Using the maceration procedure, the power of the plant's shade dried leaves was extracted with chloroform water and ethanol. The antioxidant activities of the resulting extracts were evaluated using 2 techniques: nitric oxide scavenging activity and ferric chloride reductive ability. The alcoholic extract in 600 mg/ml and 800 mg/ml and 1000 mg/ml concentration has demonstrated antioxidant activity higher than ascorbic acid (20 mg) by nitric oxide scavenging method. By using a ferric chloride scavenging model, the aqueous and alcoholic extracts at 400 g/ml and 600 g/ml concentrations revealed antioxidant activity near to that of ascorbic acid (20 g).

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