scholarly journals INSIGHT INTO BASELINE TROUBLES AND PROBLEM RESOLUTIONS IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2020 ◽  
pp. 35-38
Author(s):  
ATOLE D ◽  
DEOKATE UA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pharmaceutical Industry ◽  
Chromatographic Analysis ◽  
High Performance ◽  
Baseline Drift ◽  
High Baseline ◽  
Analytical Section ◽  
Quantitative Chromatographic Analysis ◽  
Insight Into

For pharmaceutical industry, HPLC is more than 40 years old. Even after instrumental advancements in HPLC problems still arise, troubleshooting still comes. HPLC is the main stay of analytical section nowadays in pharmaceutical industry whether it is API industry or formulation plant. Best approach for troubleshooting HPLC problems is going with systematic way. At the start of quantitative chromatographic analysis, the first parameter of paramount importance is baseline, especially for measuring the area of any given peak. Baseline usually suffers from these errors, namely, high baseline drift, periodic baseline fluctuation, and spikes. This article aims at suggesting some working methodologies which will resolve as well as avoid cases of such errors and failures.

Application of "high-performance" liquid chromatography to the study of sphingolipidoses.

1980 ◽  
Vol 26 (10) ◽  
pp. 1499-1503 ◽  
Author(s):  
M D Ullman ◽  
R E Pyeritz ◽  
H W Moser ◽  
D A Wenger ◽  
E H Kolodny
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plasma Exchange ◽  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Urine Sediment ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract Quantitative high-performance liquid chromatographic analysis of perbenzoylated sphingolipids has been used to study the correlations of body chemistry to clinical phenomena. Plasma sphingolipids were isolated from 32 Gaucher (β-glucosidase deficiency) and six Fabry (α-galactosidase deficiency) patients by solvent partition and chromatographic separation on silicic acid columns. Plasma sphingolipids from a patient undergoing plasma-exchange were separated from interfering lipids with reversed-phase columns. Liquid-chromatographic analysis of sphingolipids provides useful supportive information for diagnoses because affected individuals are shown to possess increased circulating concentrations of the pathognomonic sphingolipid. We also used this technique to monitor sphingolipid concentrations in plasma and urine sediment during plasma exchange of a p atient with Fabry’s disease. Regular plasma exchanges produced and maintained decreased concentrations of sphingolipids in plasma, but near pre-exchange concentrations were observed within days after the therapy was terminated.

scholarly journals Pentosidine as a Measure of Chronological Age in Wild Birds

The Auk ◽  
2003 ◽  
Vol 120 (2) ◽  
pp. 394-399 ◽  
Author(s):  
Richard C. Chaney ◽  
Kenneth P. Blemings ◽  
James Bonner ◽  
Hillar Klandorf
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Spectrophotometric Method ◽  
High Performance ◽  
Chronological Age ◽  
Reverse Phase ◽  
Nonenzymatic Glycation ◽  
Species Survival ◽  
Insight Into ◽  
Skin Samples

Abstract Birds have few reliable indicators of aging. Pentosidine is a product of nonenzymatic glycation that accumulates in tissues of an animal over its lifespan. The intent of this study was to determine if accumulation of skin pentosidine in birds of known ages changed as a function of time. Skin samples were obtained from the breast of 45 birds of various species obtained from the national aviary. In addition, foot webbing samples were obtained from 17 California Gulls (Larus callifornicus) of known ages. Collagen was measured by a hydroxypro-line spectrophotometric method and pentosidine was quantified using reverse phase high-performance liquid-chromatography. Pentosidine concentration in the skin and foot webbing increased linearly with age (P < 0.001). Hydroxyproline concentrations from the foot webbing were comparable to that measured in the skin; however, pentosidine concentrations were approximately one-fourth of that in skin. Knowledge regarding longevity of birds could provide information for species survival programs and insight into variations in longevity of an entire population.

Dissolvable Mg/Al layered double hydroxides and surfactant as an extractant for trace analysis of benzoylurea insecticides by high performance liquid chromatography

2020 ◽  
Vol 12 (44) ◽  
pp. 5380-5391
Author(s):  
Yanawath Santaladchaiyakit ◽  
Supalax Srijaranai
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Analysis ◽  
Benzoylurea Insecticides ◽  
Double Hydroxides ◽  
Liquid Chromatographic ◽  
Preconcentration Method

A simple and rapid preconcentration method using dissolvable Mg/Al LDHs and SDS has been demonstrated for high performance liquid chromatographic analysis of benzoylurea insecticides in water and honey samples.

Computer-aided optimization of high-performance liquid chromatography in the pharmaceutical industry

1989 ◽  
Vol 485 ◽  
pp. 183-193 ◽  
Author(s):  
E.P. Lankmayr ◽  
W. Wegscheider ◽  
J.C. Gfeller ◽  
N.M. Djordjevic ◽  
B. Schreiber
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pharmaceutical Industry ◽  
High Performance ◽  
Computer Aided

scholarly journals Ionic liquids enable accurate chromatographic analysis of polyelectrolytes

2015 ◽  
Vol 51 (52) ◽  
pp. 10551-10553 ◽  
Author(s):  
Kosuke Kuroda ◽  
Hiroyuki Ohno
Keyword(s):  
High Performance Liquid Chromatography ◽  
Ionic Liquids ◽  
Liquid Chromatography ◽  
Chromatographic Analysis ◽  
High Performance ◽  
Accurate Analysis

The use of high performance liquid chromatography with ionic liquids as eluents enables the accurate analysis of polyelectrolytes without false results.

scholarly journals Validation of analytical methodology for quantification of cefazolin sodium pharmaceutical dosage form by high performance liquid chromatography to be applied for quality control in pharmaceutical industry

2014 ◽  
Vol 50 (1) ◽  
pp. 213-223 ◽  
Author(s):  
Tahisa Marcela Pedroso ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pharmaceutical Industry ◽  
High Performance ◽  
Fractional Factorial ◽  
Chromatography Method ◽  
Pharmaceutical Dosage Form ◽  
Cefazolin Sodium ◽  
Liquid Chromatography Method

A reversed-phase high performance liquid chromatography method was validated for the determination of cefazolin sodium in lyophilized powder for solution for injection to be applied for quality control in pharmaceutical industry. The liquid chromatography method was conducted on a Zorbax Eclipse Plus C18 column (250 x 4.6 mm, 5 μm), maintained at room temperature. The mobile phase consisted of purified water: acetonitrile (60: 40 v/v), adjusted to pH 8 with triethylamine. The flow rate was of 0.5 mL min-1 and effluents were monitored at 270 nm. The retention time for cefazolin sodium was 3.6 min. The method proved to be linear (r2=0.9999) over the concentration range of 30-80 µg mL-1. The selectivity of the method was proven through degradation studies. The method demonstrated satisfactory results for precision, accuracy, limits of detection and quantitation. The robustness of this method was evaluated using the Plackett–Burman fractional factorial experimental design with a matrix of 15 experiments and the statistical treatment proposed by Youden and Steiner. Finally, the proposed method could be also an advantageous option for the analysis of cefazolin sodium, contributing to improve the quality control and to assure the therapeutic efficacy.

Determination of p-Phenylenediamine Antiozonant in Polychloroprene Rubber

1984 ◽  
Vol 57 (4) ◽  
pp. 804-812 ◽  
Author(s):  
Noel M. Potter ◽  
Ram K. S. Mehta ◽  
Michael G. Wyzgoski
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Curing Conditions ◽  
Its Analysis ◽  
Mixing Sequence ◽  
Insight Into ◽  
The Way

Abstract Elastomers susceptible to ozone cracking can be protected by the addition of antiozonants. For polychloroprene (CR) rubber, a mixture of diaryl-p-phenyl-enediamines, Wingstay 100 (WS 100), is one of the most effective antiozonants. Preliminary studies in our laboratory have shown that the effectiveness of WS 100 can be improved even further by the use of an organobentonite clay. To provide more insight into the way in which this antiozonant provides resistance to ozone cracking, a technique for its analysis was required. High performance liquid chromatography (HPLC) is an attractive technique for analyzing WS 100, as well as for a wide variety of additives in polymers. This study describes a newly developed method for the determination of WS 100 in CR. The method is based on acetonitrile extraction of the antiozonant from the rubber, followed by quantitative analysis using HPLC. With this method, subtle differences in concentrations of WS 100 in CR, caused by changes in compounding, mixing sequence, curing conditions, and grease extraction, have been measured.

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