scholarly journals PREPARATION OF POWDER FROM BROWN SEAWEED (SARGASSUM PLAGYOPHYLLUM) BY FREEZE-DRYING WITH MALTODEXTRIN AS A STABILIZER

2018 ◽  
Vol 10 (1) ◽  
pp. 348
Author(s):  
Effionora Anwar ◽  
Hilmia Erianto ◽  
Kurnia Sari Setio Putri
Keyword(s):  
Water Content ◽  
Freeze Drying ◽  
Storage Time ◽  
Storage Temperature ◽  
Brown Seaweed ◽  
Drying Process ◽  
Dextrose Equivalent ◽  
The Stability ◽  
And Storage ◽  
Lower Water Content

Objective: The aim of this study was to prepare powder from liquid extract with maltodextrin dextrose equivalent 10–15 as a stabilizer using a freezedryingmethod to maintain stability during drying process and extend storage time.Methods: Powders were prepared for four formulas: F1 (without maltodextrin), F2 (2.5% maltodextrin), F3 (5% maltodextrin), and F4 (10%maltodextrin). Powder from the four formulas was characterized by its phlorotannin concentration, antioxidant activity, water content, morphology,particle size distribution, pH, and organoleptic activities.Results: F4 was the best formula because it contained the highest phlorotannin concentration (113.06±1.36) or 0.25%, highest percentage ofinhibition concentration50 (IC50) (4.06% at a concentration of 5000 ppm), and lowest water content (5.16%); moreover, in a stability test, F4 exhibiteda more stable phlorotannin concentration and lower water content than F1, with an optimal storage temperature of 4°C.Conclusion: Maltodextrin can improve the stability bioactive compounds during the freeze-drying process and storage time.

scholarly journals Stability of pseudorabies virus during freeze-drying and storage: effect of suspending media

1976 ◽  
Vol 4 (1) ◽  
pp. 1-5
Author(s):  
E M Scott ◽  
W Woodside
Keyword(s):  
Freeze Drying ◽  
Pseudorabies Virus ◽  
Storage Effect ◽  
Drying Process ◽  
Sodium Glutamate ◽  
Degree Of Protection ◽  
The Stability ◽  
Subsequent Storage ◽  
And Storage ◽  
Lactalbumin Hydrolysate

The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various combinations of sucrose, glutamate, and potassium phosphates. Suspending media containing glutamate, either alone or in combination with sucrose and either dextran or phosphates, afforded the greatest degree of protection during the freeze-drying process and upon storage. Some possible functions of these additives in preventing injury to the virus during freezing and drying have been suggested.

scholarly journals Germination and Water Content of Cocoa Seeds After Storage Treatments

2018 ◽  
Vol 34 (3) ◽  
pp. 146-155
Author(s):  
Indah Anita-Sari ◽  
Bayu Setyawan ◽  
Agung Wahyu Susilo
Keyword(s):  
Shelf Life ◽  
Water Content ◽  
Storage Temperature ◽  
Seed Viability ◽  
Vacuum Packaging ◽  
Recalcitrant Seed ◽  
R Program ◽  
Randomized Design ◽  
The Stability ◽  
And Storage

The challenge of providing cocoa seeds is the characteristic of recalcitrant seed, thus requiring storage techniques that can slow down the decline in seed viability in an effort to manage the distribution of cocoa seeds which depend to the time of demand and harvest. The research aimed to study the effect ofpackaging method for storage of cocoa seeds; the effect of shelf life and temperature to germination and water content of seeds, and the stability of seed germination at different age and storage temperature. The research was conducted at Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute, Jember, East Java. The research used complete randomized design with research design using split plot. The treatments consisted of packaging methods, storage temperature and storage life. Packaging methods consisted of two methods, i.e. vacuum methods and non vacuum methods. Storage temperature consisted of three treatments, i.e. 15OC, 25OC and 30OC. both of packaging and temperature treatments were stored at four shelf life treatment included 1 week, 2 weeks, 3 weeks, and 4 weeks. Each treatment was repeated with three replications and each treatment per replication consisted of 50 seeds. Data analysis used SAS 9.0 and stability analysis used R program and PB Tool. Water content of seeds, germination of seeds and number of seedy seeds were observed. The results showed that type of packaging and storage temperature effect to germination of cocoa seeds but did not effect to seeds water content. Storage of cocoa seeds by non-vacuum packaging showed a higher of germination compared to vacuum packaging in all temperature treatments. Storage with non vacuum packaging at a temperature 25OC showed a germination more than 85% for 2 weeks of shelf life, while storage of cocoa seeds at a temperature of 30OC had a germination of more than 85% for only 1 week shelf life. Storage of cocoa seed at 15OC showed the lowest of germination at treatment. Storage treatments did not show effect on water content of cocoa seeds.

Viability, Efficacy, and Storage Stability of Freeze-Dried Biocontrol Agent Candida sake Using Different Protective and Rehydration Media

2001 ◽  
Vol 64 (6) ◽  
pp. 856-861 ◽  
Author(s):  
M. ABADIAS ◽  
N. TEIXIDÓ ◽  
J. USALL ◽  
A. BENABARRE ◽  
I. VIÑAS
Keyword(s):  
Freeze Drying ◽  
Storage Stability ◽  
Storage Temperature ◽  
Penicillium Expansum ◽  
Freeze Dried ◽  
Skimmed Milk ◽  
The Stability ◽  
Candida Sake ◽  
Initial Starting ◽  
And Storage

Viability, efficacy against Penicillium expansum on Golden Delicious apples, and storage stability of freeze-dried Candida sake strain CPA-1 were studied. The effect of several protective agents and rehydration media was investigated in the freeze drying of C. sake. Skimmed milk at 10% concentration was a good rehydration medium for all protectants tested. In general, good viability results were obtained when the same solution was used as a protectant and as a rehydration medium. The best survival was obtained when C. sake cells were protected with 10% lactose + 10% skimmed milk and rehydrated with skimmed milk (85% viability). The potential for biocontrol of the best freeze-dried treatments against P. expansum on apples was compared with that of fresh cells. Freeze-dried treatments at 1 × 107 CFU/ml reduced the incidence of decay by 45 to 66%. The best biocontrol effect was obtained with cells that had been freeze dried using 10% lactose + 10% skimmed milk as a protectant and 1% peptone as a rehydration medium, with a 66% reduction in rot incidence. However, in all treatments, the efficacy of freeze-dried cells was significantly lower than fresh cells. The stability of freeze-dried samples decreased during storage and was influenced by storage temperature. In the best treatment, storage of C. sake cells for 60 days at 4°C resulted in final concentrations of 2.5 × 108 CFU/ml, which was a 10-fold reduction in relation to the initial starting concentration of cells prior to freeze drying.

scholarly journals Flow-cytometric assessment of damages to Acetobacter senegalensis during freeze-drying process and storage

2013 ◽  
Vol 2 (1s) ◽  
pp. 10 ◽  
Author(s):  
Rasoul Shafiei ◽  
Frank Delvigne ◽  
Phillipe Thonart
Keyword(s):  
Freeze Drying ◽  
Storage Temperature ◽  
Culture Media ◽  
Cell Envelope ◽  
Storage Conditions ◽  
Cell Multiplication ◽  
Drying Process ◽  
Log Reduction ◽  
Downstream Processes ◽  
And Storage

Downstream processes have great influences on bacterial starter production. Different modifications occur to cellular compounds during freeze-drying process and storage of bacterial starters. Consequently, viability and culturability (multiplication capacity) undergo some changes. In this study, the effects of freeze-drying process and storage conditions were examined on cell envelope integrity, respiration and culturability of <em>Acetobacter senegalensis</em>. Freezing of cells protected with mannitol (20% w/w) did not affect cell multiplication and respiration considerably; however, 19% of cells showed compromised cell envelope after freezing. After drying, 1.96&times;10<sup>11</sup> CFU/g were enumerated, indicating that about 34% of the cells could survive and keep their culturability. Drying of the cells induced further leakage in cell envelope and finally 81% of cells appeared as injured ones; however, 87% of the dried cells maintained their respiration capacity. Storage temperature had significant effect on cell multiplication ability; higher storage temperature (35&deg;C) caused 8.59-log reduction in cell culturability after nine-month period of storage. Collapse of cell envelop integrity and respiration was observed at 35&deg;C. At lower storage temperature (4&deg;C), the culturability decreased about one-log reduction after nine months. Cell envelope integrity was subjected to minor changes during a period of nine month-storage at 4&deg;C whereas a heterogeneous population of cells with different respiration capacity emerged at 4&deg;C. These results indicate that a major part of cells undergone drying process and storage entered into viable but non-culturable state. In addition, usage of different culture media didn&rsquo;t improve resuscitation. Besides, it seems that sub-lethal damages to cell envelope caused uptake of propidium iodide, however these kinds of injuries could not impress cell multiplications and respiration.

Effect of ginseng polysaccharide on the stability of lactic acid bacteria during freeze-drying process and storage

2006 ◽  
Vol 29 (9) ◽  
pp. 735-740 ◽  
Author(s):  
Seung-Hyun Yang ◽  
Sung-Hoon Seo ◽  
Sang-Wook Kim ◽  
Seung-Ki Choi ◽  
Dong-Hyun Kim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Freeze Drying ◽  
Drying Process ◽  
Ginseng Polysaccharide ◽  
The Stability ◽  
And Storage

scholarly journals Effects of heat treatment and storage temperature on the use of açaí drink by nutraceutical and beverage industries

2012 ◽  
Vol 32 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Tatiane Regina Albarici ◽  
José Dalton Cruz Pessoa
Keyword(s):  
Temperature Effect ◽  
Storage Temperature ◽  
Life Time ◽  
Reaction Rate Constant ◽  
Anthocyanin Content ◽  
Order Kinetic ◽  
First Order ◽  
The Stability ◽  
And Storage ◽  
Anthocyanin Degradation

This study assesses the storage temperature effect on the anthocyanins of pasteurized and unpasteurized açaí pulp. The data was obtained using a pasteurized and lyophilized pulp (PLP) to evaluate the temperature effect (0, 25, and 40 °C). Part of non-pasteurized frozen pulp (NPP) was pasteurized (NPP-P) at 90 °C for 30 seconds; both pulps were stored at 40 °C. The anthocyanin content reduction in the drink was evaluated from the half-life time (t1/2), activation energy (Ea), temperature quotient (Q10), and the reaction rate constant (k). The t1/2 of the PLP anthocyanins stored at 40 °C was 1.8 times less than that stored at 25 °C and 15 times less than that stored at 0 °C; therefore, the higher temperatures decreased the stability of anthocyanins. The pasteurization increased the t1/2 by 6.6 times (10.14 hours for NPP and 67.28 hours for NPP-P). The anthocyanin degradation on NPP-P followed a first order kinetic, while NPP followed a second order kinetic; thus it can be said that the pasteurization process can improve the preservation of anthocyanins in the pulp.

scholarly journals Effects of storage temperature, storage time, and Cary-Blair transport medium on the stability of the gut microbiota

2019 ◽  
Vol 13 (5) ◽  
pp. 256-260
Author(s):  
Naoyoshi Nagata ◽  
Mari Tohya ◽  
Fumihiko Takeuchi ◽  
Wataru Suda ◽  
Suguru Nishijima ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Storage Time ◽  
Storage Temperature ◽  
Transport Medium ◽  
The Stability ◽  
Temperature Storage

Stability of Bortezomib 1-mg/mL Solution in Plastic Syringe and Glass Vial

2005 ◽  
Vol 39 (9) ◽  
pp. 1462-1466 ◽  
Author(s):  
Pascal André ◽  
Salvatore Cisternino ◽  
Fouad Chiadmi ◽  
Audrey Toledano ◽  
Joël Schlatter ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Color Change ◽  
Hplc Method ◽  
Initial Concentration ◽  
Plastic Syringe ◽  
The Mean ◽  
The Stability ◽  
And Storage ◽  
Mean Concentration ◽  
Antineoplastic Chemotherapy

BACKGROUND: The proteasome inhibitor bortezomib (BTZ), used in antineoplastic chemotherapy, must be diluted in NaCl 0.9% for injection and stored for no more than 3 hours in a syringe or 8 hours in a vial. Better information on its stability could improve storage. OBJECTIVE: To assess the stability of BTZ solution (1 mg/mL) in syringes and vials. METHODS: BTZ 1-mg/mL solutions were prepared by adding sterile NaCl 0.9% to Velcade vials containing 3.5 mg of lyophilized BTZ. Syringes were filled with 1 mL of solution and stored in the dark at 5 °C or 60 °C; others were not protected from light and stored at 22 °C. Velcade vials containing 1 mL of solution were stored at 5 °C in the dark. Samples were taken at various times over 23 days and assayed in duplicate. An HPLC method for assaying the stability of BTZ was validated. Appearance and pH were recorded. RESULTS: There was no color change or precipitation in the samples, and the pH was stable. Oxidation, light, and storage temperature all affected the chemical stability of BTZ. The mean concentrations of BTZ in syringes stored for 2, 3, and 5 days at 60, 22, and 5 °C were >95% of the initial concentration. The mean concentration of BTZ in vials stored for 5 days at 5 °C was >95% of the initial concentration. CONCLUSIONS: BTZ stored refrigerated in vials or syringes and protected from light is chemically stable for 5 days after reconstitution.

Sign in / Sign up

Export Citation Format

Share Document