IDENTIFICATION OF CHEMICAL COMPOUNDS AND ANTIBACTERIAL ACTIVITY OF 96% ETHANOL EXTRACT FROM MORINGA OLEIFERA LAM. LEAVES AGAINST MRSA (METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS)

2021 ◽  
pp. 111-114
Author(s):  
NURSANNA IRAWATY SINAGA ◽  
MUHAMMAD HANAFI ◽  
NOVI YANTIH
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Methicillin Resistant ◽  
Activity Test ◽  
Moringa Oleifera Lam

Objective: Infection with antibiotic-resistant organisms, requiring the selection of the right drug to fight these organisms. Moringa oleifera Lam. leaves as natural ingredients have MRSA (Methicillin-Resistant Staphylococcus aureus) antibacterial activity. The purpose of this study is to identify the chemical compounds and to determine the antibacterial activity from the 96% ethanol extract of Moringa oleifera Lam. leaves against MRSA bacteria. Methods: The Moringa oleifera Lam. leaves were extracted by maceration using 96% (v/v) ethanol. Mass spectrometry was performed on an LC-MS/MS Xevo, G2-XS QTof (Waters MS Technologies) to identified chemical compounds from the extract. The ionization type is ESI. The method of the antibacterial activity test was using agar paper disc diffusion. Antibacterial activity was based on the diameter of the bacterial inhibition zone. Results: The result of the antibacterial activity test for 96% ethanol extract of Moringa oleifera Lam. leaves a concentration of 10, 20, and 40 % each has inhibition diameter was 10, 13, and 15.5 mm, and for linezolid as a positive control at 30µg has diameter inhibition was 20 mm, ethanol 96% as a negative control was 0 mm. The 96% ethanol extract of Moringa oleifera Lam. leaves contains 13-hydroxy-9,11-hexadecadienoic acid, 3-tert-butyl-4-methoxyphenol, bistortaside, daturametelin H, digiprolactone, ephedradine C, kaempferol-3-O-rutinoside,kaempferol-3-O-β-D-glucopyranoside, kaempferol-7-O-α-L rhamnoside, phenyl propionic acid, pyrophaeophorbide A, quercetin, stearidonic acid, stigmastan-3,6-dione. Conclusion: The 96% ethanol extract of Moringa oleifera Lam. leaves contains 14 compounds. The 96% ethanol extract of Moringa oleifera Lam. leaves have activity against MRSA bacteria. The antibacterial effect of the extract increased with an increase in its concentration. The extract exerted a greater antibacterial effect on the concentration 40%.

scholarly journals Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) of Ethanol Extract of God's Crown (Phaleria macrocarpa) Leaves, Peel, and Fruit Flesh

2020 ◽  
Vol 31 (2) ◽  
pp. 81
Author(s):  
Rani Afifah Nur Hestiyani ◽  
Tri Okmawati Handini
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Methicillin Resistant ◽  
Fruit Flesh ◽  
Phaleria Macrocarpa ◽  
Antibiotic Control

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that becomes the main concern since it is a multidrug-resistant organism and causes high morbidity and mortality. This study aimed to investigate the antibacterial activity of ethanolic extract of Phaleria macrocarpa leaves, peel, and fruit flesh on MRSA. This study was an experimental laboratory study with a post-test only control group design to assess the antibacterial activity of ethanolic extract from leaves, peel, and fruit flesh of Phaleria macrocarpa against MRSA ATCC 43300 and MRSA clinical isolates using a disk diffusion method. Extracts from the leaves, peel, and flesh of Phaleria macrocarpa had potential as an antibacterial agent against MRSA ATCC 43300 at a concentration of 40%, although not yet equivalent to antibiotic control. The ethanol extract of Phaleria macrocarpa peel at a concentration of 30-40% had potential as an antibacterial agent against MRSA clinical isolates, although not yet equivalent to antibiotic control. Further research needs to be performed so that Phaleria macrocarpa extract can be a potential source of subsequent antibacterial development against MRSA.

scholarly journals In vitro Activity of Rambutan Binjai (Nephelium lappaceum) Peel Extract from Indonesia to Methicillin-Resistant Staphylococcus aureus (MRSA)

2018 ◽  
Vol 17 (2) ◽  
pp. 197-203
Author(s):  
Tina Rostinawati ◽  
Ami Tjitraresmi ◽  
Myra Vania Wisnuputri
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Nephelium Lappaceum ◽  
New Compounds ◽  
Peel Extract

Methicillin-resistant Staphylococcus aureus (MRSA) is the most common bacteria causing nosocomial infections with high levels of resistance to available antibiotics. So, it is necessary to search for new compounds to solve this problem. Various studies showed antibacterial activity of rambutan peel but for Rambutan Binjai peel extract that are from Indonesia has never been studied against the MRSA. This study aims to determine the antibacterial activity, the value of minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) using agar diffusion method. The concentration of rambutan peel ethanol extract at as much as 62.5 mg/ml showed the inhibitory diameter i.e 21.3 ± 2.4 mm. MIC and MBC were in the same range, which was between 0.98 (mg/ml) to 1.95 (mg/ml). The activity strength of tetracycline against the extract was at 1:50. This revealed that Rambutan Binjai peel extract had great potency as antibacterial agent to MRSA. Dhaka Univ. J. Pharm. Sci. 17(2): 197-203, 2018 (December)

scholarly journals Antibacterial activity testing of ethanolic extract of aloe vera leaf and gel against methicillin-resistant Staphylococcus aureus

2014 ◽  
Vol 26 (2) ◽  
Author(s):  
Tias Eka Pratiwi ◽  
Warta Dewi ◽  
Eddy Prijono
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Test Method ◽  
Methicillin Resistant ◽  
Aloe Vera Gel ◽  
Significant Difference

Introduction: The interest on Aloe vera based herbal products is fast growing during recent years, particularly in medication regarding their antibacterial properties which had a lot of active components such as saponin and anthraquinone. Methicillin-resistant Staphylococcus aureus has emerged as one of the most important human pathogens causing nosocomial infections and became the first priority as a consequence of their resistancy. The purpose of this study is to determine the minimum levels of ethanol extract of leaves and Aloe Vera gel that can inhibit MRSA and find out whether there are differences in antibacterial power between of ethanol extract of leaves and Aloe Vera gel. Methods: Ten isolates of MRSA were investigated for their sensitivity to Aloe vera leaf and gel extract using the serial dilution method by doing two times repetition and statistically analyzed according to t-test method. Result: The result shows that the Minimum Inhibitory Concentration of Aloe vera leaf and gel extract each located at a concentration of 25% and 12,5%. There is a significant difference in antibacterial power between the ethanol extract of leaves and Aloe Vera gel in inhibiting MRSA with MIC values of 25% and 12.5% Conclusion: There was a difference between Aloe vera leaf and gel extract antibacterial activity, where the gel extract is more effective than leaf extract.

scholarly journals Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 112
Author(s):  
Theresia H. Tunas ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Moringa Oleifera Leaves ◽  
Inhibitory Power ◽  
Moringa Oleifera Lam

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.

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