EFFICACY OF BINARY COMBINATION OF DELTAMETHRIN+MGK-264 ON LEVELS OF BIOCHEMICAL CHANGES IN THE SNAIL LYMNAEA ACUMINATA

Objective: The objective of the present study is the evaluation of the effect of the sublethal (40% and 60% of 48h LC50) binary combination (1:5 ratios) of molluscicides deltamethrin+MGK-264 on the endogenous levels of protein, amino acid and nucleic acid in different tissues of snail Lymnaea acuminata. Methods: The snails were treated with 1:5 mixtures of sub-lethal concentration of (40% and 60% of 48h LC50) deltamethrin+MGK-264 on the protein, amino acid and nucleic acid levels in gonadal, nervous and foot tissue of L. acuminata. In order to study the effect of withdrawal from treatment, the snails were first exposed to the above concentrations for 96h, after which they were transferred to freshwater. Water was changed every 24h for the next seven days, after which different biochemical parameters were estimated. Results: There was a significant change in the levels of protein (sublethal concentration of 60% of 48h LC50 after 96h) gonadal, nervous and foot tissues are 48.1, 12.1 and 14.5%, respectively, amino acid are 273, 234 and 252%, respectively, DNA are 25.1, 38.9 and 42.1%, respectively and RNA are 12.2, 30.7 and 30.5%, respectively. These changes were time and concentration-dependent. In the withdrawal experiment, the snails were treated for 96h to transfer in freshwater for 7 d, which caused significant recovery in all the biochemical parameters. Conclusion: The present study concluded that the high molluscicidal activity of deltamethrin+MGK-264 simultaneous decrease in the levels of proteins, DNA, RNA and increase in the level of amino acids.

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Effects of Monocrotophos on Some Biochemical Parameters in Fresh Water Fish Oreochromis Mossambicus (Tilapia)

GIS Business ◽

10.26643/gis.v14i6.11702 ◽

2019 ◽

Vol 14 (6) ◽

pp. 224-229

Author(s):

A. Solaiappan ◽

J. Prakash Sahaya Leon

Keyword(s):

Amino Acid ◽

Biochemical Parameters ◽

Amino Acid Level ◽

Exposure Period ◽

Oreochromis Mossambicus ◽

Sublethal Concentration ◽

Control Group ◽

Fresh Water Fish ◽

Tissue Samples ◽

Water Fish

The toxic effect of Monocrotophos on some biochemical parameters such as Protein and Amino acid in freshwater fish Oreochromis mossambicus were studied. The fishes exposed to 1/10th, 1/20th, and 1/30th sublethal concentration of Monocrotophos for 30 days. The tissue samples of gill, liver were analysed at the end of the 30th day exposure period. A decreased value of Protein and Amino acid were observed in 1/10th, 1/20th, and 1/30th sub lethal concentration of Monocrotophos at the exposure period of 30 days while comparing with the control group. The high variation observed in the 1/10th sublethal level of 30thday exposure. While compare with control group the effects of Monocrotophos on fish found during this experiment showed that the level of protein and amino acid level in various tissues (gill and liver) of Oreochromis mossambicus decreased.

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Differentiation of Chronic and Aggressive Periodontitis by FTIR Spectroscopy

Journal of Dental Research ◽

10.1177/0022034516663696 ◽

2016 ◽

Vol 95 (13) ◽

pp. 1472-1478 ◽

Cited By ~ 7

Author(s):

N. Simsek Ozek ◽

I. Zeller ◽

D.E. Renaud ◽

P. Gümüş ◽

N. Nizam ◽

...

Keyword(s):

Lactic Acid ◽

Amino Acid ◽

Nucleic Acid ◽

Ftir Spectroscopy ◽

Acid Content ◽

Smoking Status ◽

Spectral Band ◽

Aggressive Periodontitis ◽

Nucleic Acid Content ◽

Protein Amino Acid

Without longitudinal clinical data, it is difficult to differentiate some cases of chronic periodontitis (CP) and aggressive periodontitis (AgP). Furthermore, both forms of disease are exacerbated by tobacco use. Therefore, this cross-sectional study was planned, primarily, to determine the ability of Fourier-transform infrared (FTIR) spectroscopy to distinguish CP and AgP patients by analysis of human saliva samples and, secondarily, to assess the potential confounding influence of smoking on discriminating disease-specific spectral signatures. FTIR spectra were collected from patients with a clinical diagnosis of CP ( n = 18; 7 smokers) or AgP ( n = 23; 9 smokers). Self-reported smoking status, which may be unreliable, was confirmed by salivary cotinine analysis. Spectral band area analysis and hierarchical cluster analyses were performed to clarify if the 2 periodontitis groups as well as smoker and nonsmoker patients could be differentiated from each other. Significant variations in lipid, amino acid, lactic acid, and nucleic acid content were found between nonsmoker CP and AgP groups. Although significantly lower lipid, phospholipid, protein, amino acid, lactic acid, and nucleic acid content was noted in the smoker AgP group compared with the nonsmoker AgP group, in the CP group, phospholipid, protein, amino acid, and lactic acid content was significantly lower for smokers compared with the nonsmokers. Based on these variations, nonsmoker CP and AgP patients were discriminated from each other with high sensitivity and specificity. Successful differentiation was also obtained for the smoker CP and AgP groups. Thiocyanate levels successfully differentiated smokers from nonsmokers, irrespective of periodontal status, with 100% accuracy. Differentiation of AgP and CP forms, concomitant with determination of smoking status, may allow the dental health professional to tailor treatment accordingly.

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KEC: unique sequence search by K-mer exclusion

Bioinformatics ◽

10.1093/bioinformatics/btab196 ◽

2021 ◽

Author(s):

Pavel Beran ◽

Dagmar Stehlíková ◽

Stephen P Cohen ◽

Vladislav Čurn

Keyword(s):

Amino Acid ◽

Nucleic Acid ◽

Source Code ◽

Unique Sequence ◽

Supplementary Information ◽

Supplementary Data ◽

Laptop Computers ◽

Sequence Search ◽

Target Sequences ◽

Cross Reference

Abstract Summary Searching for amino acid or nucleic acid sequences unique to one organism may be challenging depending on size of the available datasets. K-mer elimination by cross-reference (KEC) allows users to quickly and easily find unique sequences by providing target and non-target sequences. Due to its speed, it can be used for datasets of genomic size and can be run on desktop or laptop computers with modest specifications. Availability and implementation KEC is freely available for non-commercial purposes. Source code and executable binary files compiled for Linux, Mac and Windows can be downloaded from https://github.com/berybox/KEC. Supplementary information Supplementary data are available at Bioinformatics online.

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