Effects of Monocrotophos on Some Biochemical Parameters in Fresh Water Fish Oreochromis Mossambicus (Tilapia)

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 224-229
Author(s):  
A. Solaiappan ◽  
J. Prakash Sahaya Leon
Keyword(s):  
Amino Acid ◽  
Biochemical Parameters ◽  
Amino Acid Level ◽  
Exposure Period ◽  
Oreochromis Mossambicus ◽  
Sublethal Concentration ◽  
Control Group ◽  
Fresh Water Fish ◽  
Tissue Samples ◽  
Water Fish

The toxic effect of Monocrotophos on some biochemical parameters such as Protein and Amino acid in freshwater fish Oreochromis mossambicus were studied. The fishes exposed to 1/10th, 1/20th, and 1/30th sublethal concentration of Monocrotophos for 30 days. The tissue samples of gill, liver were analysed at the end of the 30th day exposure period. A decreased value of Protein and Amino acid were observed in 1/10th, 1/20th, and 1/30th sub lethal concentration of Monocrotophos at the exposure period of 30 days while comparing with the control group. The high variation observed in the 1/10th sublethal level of 30thday exposure. While compare with control group the effects of Monocrotophos on fish found during this experiment showed that the level of protein and amino acid level in various tissues (gill and liver) of Oreochromis mossambicus decreased.

Enzyme modulation studies of Fumaronitrile on fresh water fish Oreochromis mossambicus

2016 ◽  
Vol 5 (11) ◽  
pp. 5050
Author(s):  
Chinnadurai Kuppusamy ◽  
Arul Ganesh ◽  
Karthikeyeni Sambanthan ◽  
Eyini Muthukumarasamy ◽  
Balaji Paulraj*
Keyword(s):  
Antioxidant Enzymes ◽  
Fresh Water ◽  
Enzyme Activities ◽  
Enzyme Assay ◽  
Oreochromis Mossambicus ◽  
Control Group ◽  
Fresh Water Fish ◽  
Treatment Groups ◽  
Enzyme Modulation ◽  
Water Fish

The present study was directed to analyse the potential enzymatic variation efficiency of fumaronitrile on fresh water fish tilapia (Oreochromis mossambicus). To study the acute toxicity of xenobiotic five different concentrations (20, 40, 60, 80 and 100ppb) of fumaronitrile along with control group was maintained for over 96 hrs. At every 1 hr. fishes was monitor both control and treatment groups. The LC50 value was found to be as 60ppb. To study the chronic toxicity of xenobiotic three different concentrations (2ppb, 4ppb and 6ppb) of fumaronitrile along with control group was maintained for over 100 days. At 60, 80,100 days fishes was sacrificed both control and treatment groups and the organs such as liver were collected to analyse the changes in testicular enzyme activities and an increasing pattern for both antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) was observed. Enzyme assay is one of the important methods to evaluate the toxicity of xenobiotic. At present, no report is available on the effect of fumaronitrile on antioxidant enzymes and testicular enzyme activities. This study focus light on the effects of fumaronitrile in antioxidant enzymes CAT, SOD and testicular enzymes ALP, ACP, LDH and SDH in Oreochromis mossambicus.

scholarly journals SUPPLEMENTATION OF CALCIUM AND SELENIUM AGAINST CADMIUM INDUCED BIOACCUMULATION IN SELECTED TISSUES OF FRESH WATER FISH, OREOCHROMIS MOSSAMBICUS

2019 ◽  
pp. 92-98
Author(s):  
Obaiah Jamakala
Keyword(s):  
Fresh Water ◽  
Kidney Tissue ◽  
Body Burden ◽  
Aquatic Organisms ◽  
Oreochromis Mossambicus ◽  
Sublethal Concentration ◽  
Fresh Water Fish ◽  
Protective Effects ◽  
Exposed Fish ◽  
Water Fish

Objective: Cadmium (Cd) is one of the most hazardous heavy metals in aquatic environments and could threaten aquatic organisms including fish. The present study was carried out to know the protective effects of calcium (Ca) and selenium (Se) in reducing the Cd bioaccumulation in selected tissues of fresh water fish, Oreochromis mossambicus. Methods: The fresh water fish, Oreochromis mossambicus (Tilapia) was brought from the local ponds and were allowed for acclimatization to the laboratory conditions. After acclimatization, fish were exposed to sublethal concentration of Cd (1/10th of LC50/48h, i.e., 5 ppm) for 7, 15 and 30 d (d) period. 15d Cd-exposed fish were divided into three groups. The first group of fish were subjected to Ca (1 ppm) supplementation, second group received only Se (1 ppm) supplement and third group of fish were supplemented with the combination of both Ca and Se at the above said doses and observed for 7, 15 and 30d time periods. After specific time intervals, liver, kidney, gill and intestine tissues were isolated and used for Cd bioaccumulation studies. Results: Cd concentration levels significantly (P<0.05) increased in the test tissues with increased period of exposure. Maximum Cd accumulation was found in 30d Cd-exposed fish kidney tissue (22.611±0.676 μg/gm wet wt. of the tissue). However, after supplementing with Ca and/or Se, there was a significant reversal in the levels of Cd concentration in all the test tissues. Maximum reduction was observed under Ca alone supplementation. Conclusion: The present study clearly reveals that individual supplementation of Ca tends to detoxify the Cd body burden in the test tissues than the other modes of supplementation.

scholarly journals Some Blood Biochemical Changes in Response to Saline Exposure in the Fresh Water Fish, Notopterus notopterus (Pallas)

2015 ◽  
Vol 49 ◽  
pp. 19-23 ◽  
Author(s):  
K.S. Kavya ◽  
R.S. Kulkarni ◽  
M. Jadesh
Keyword(s):  
Blood Glucose ◽  
Biochemical Parameters ◽  
Exposure Period ◽  
Saline Stress ◽  
Fresh Water Fish ◽  
Saline Condition ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Water Fish ◽  
Notopterus Notopterus

The changes in some blood biochemical parameters were studied in the freshwater fish N. notopterus under saline exposure for a longer period of 30 days. The blood biochemical parameters are glucose, protein, triglycerides and cholesterol. All these parameters exhibited increased level except of blood glucose which remained unchanged as observed after the termination of saline exposure. Thus it indicates that although fish survives and able to tolerate extreme saline condition as there was no mortality during the exposure period. The increase of blood biochemical parameters can be considered as a kind of saline stress response particularly on lipid derivatives.

scholarly journals Nutritional component of Barbonymus balleroides: A wild fresh water fish from Indonesia

2019 ◽  
Vol 20 (2) ◽  
pp. 581-588 ◽  
Author(s):  
SYAHFITRI ANITA ◽  
HARYONO HARYONO ◽  
GEMA WAHYUDEWANTORO
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Fresh Water ◽  
Native Species ◽  
Body Part ◽  
Amino Acid Profile ◽  
The Body ◽  
Fresh Water Fish ◽  
Fish Samples ◽  
Water Fish

Abstract. Anita S, Haryono, Wahyudewantoro G. 2019. Nutritional component of Barbonymus balleroides: A wild fresh water fish from Indonesia. Biodiversitas 20: 581-588. Barbonymus balleroides is a native species of fish from Indonesia that threatens by overfishing activity and habitat damage whereas little is known about its nutritional information. This study aimed to evaluate the nutritional content of wild barb fish that was collected from two different sites in Indonesia. Fishes were collected from Serayu and Cipunagara River. The body part of fishes was grouped and weighted to identify the ratio. Proximate composition was determined for flesh and byproduct parts. Flesh parts of fish were then examined for mineral content, fatty acid, and amino acid profile. Barb fish samples mainly form Cipunagara River contain high protein compare to several Indonesian freshwater fishes that commonly consumed. Flesh samples from both fish group were the heaviest part followed by its edible byproduct that often wasted. The amount of moisture and protein were higher in the flesh parts while byproduct showed higher content of fat and ash. Both samples contained a dominant amount of macro elements K and P. Fatty acid profile of flesh samples showed essential fatty acid that is good for human health. Amino acid profile showed the most dominant amino acid found in both samples was glutamic acid.

scholarly journals Effect Of Arginine On The Some Blood And Biochemical Parameters For Local Goat Females.

2019 ◽  
Vol 12 (1) ◽  
pp. 24-36
Author(s):  
A Ahmed ◽  
T Mohammed
Keyword(s):  
Amino Acid ◽  
Biochemical Parameters ◽  
Protein Concentration ◽  
White Blood Cells ◽  
Biochemical Properties ◽  
Control Group ◽  
Blood Samples ◽  
Blood Biochemical ◽  
T1 And T2 ◽  
Different Doses

This study was conducted in one of the private sector fields in Anbar province - Hit city - Albasaer village (70 km west of the Ramadi city,), for the period from 28/6/2018 to 1/9/2018. Twenty one local female goats aged between 2-4 years and weighing between 24.5 to 36.5 kg were used, Which have previous one birth or more. All female goats were tested using ultrasound to make sure they were not pregnant before the experiment began. Females were randomly divided into three equal groups (7 goats in each group).Vaginal sponges (60 mg MAP) were injected into the three groups at the same time. The first group T1 was injected intramuscularly with the amino acid, arginine (US Nevada manufacturing) in the muscles at 200μmol.kg Five days before the sponge was pulled out until the 17 day after the sponge was pulled out, While the second group T2 was injected with amino acid (arginine) at 160 μmol.kg. Five days before the sponge was pulled until the 17th day after that. The third group T3 control group was injected with 5 ml Normal Saline intramuscularly of the animal. All animals were injected three times daily from the eighth day after the sponge was placed (five days before removing of the sponge) until 17 days after the removing of sponge. Where the total number of injection days was 22 days. Blood samples were taken from the jugular vein before injection of the arginine on day 7 and day 12 of the sponge placed either after the sponge pulled the blood samples were taken on the days 2, 3, 4, 6, 9, 13, 18, respectively of the experiment After sponges removing. The objective of the study was to measure changes in the blood, biochemical parameters, during arginine treatment. The results of this study showed significant differences. T1 and T2 group were superior compared to control group in blood properties which include PCV in periods 2, 5, the number of white blood cells in periods 2, 3, 4, 5, 6, 9, MID in periods 3, 9 and Lymphocytes in periods 4, 5, 6, 9, While the neutrophil cells the period of 3. In terms of biochemical properties, the results showed a significant differences, between the treatments of T1 and T2 were superior compared to control treatments in the total protein concentration in period 6, and the globulin in period 6. We conclude that the use of different doses of arginine can improve the health status of female goats.

scholarly journals EFFICACY OF BINARY COMBINATION OF DELTAMETHRIN+MGK-264 ON LEVELS OF BIOCHEMICAL CHANGES IN THE SNAIL LYMNAEA ACUMINATA

2020 ◽  
pp. 111-116
Author(s):  
R. N. SINGH ◽  
PRADEEP KUMAR ◽  
NAVNEET KUMAR ◽  
D. K. SINGH
Keyword(s):  
Amino Acid ◽  
Nucleic Acid ◽  
Biochemical Parameters ◽  
Sublethal Concentration ◽  
Protein Amino Acid ◽  
Simultaneous Decrease ◽  
Different Tissues ◽  
Lymnaea Acuminata ◽  
Molluscicidal Activity ◽  
Binary Combination

Objective: The objective of the present study is the evaluation of the effect of the sublethal (40% and 60% of 48h LC50) binary combination (1:5 ratios) of molluscicides deltamethrin+MGK-264 on the endogenous levels of protein, amino acid and nucleic acid in different tissues of snail Lymnaea acuminata. Methods: The snails were treated with 1:5 mixtures of sub-lethal concentration of (40% and 60% of 48h LC50) deltamethrin+MGK-264 on the protein, amino acid and nucleic acid levels in gonadal, nervous and foot tissue of L. acuminata. In order to study the effect of withdrawal from treatment, the snails were first exposed to the above concentrations for 96h, after which they were transferred to freshwater. Water was changed every 24h for the next seven days, after which different biochemical parameters were estimated. Results: There was a significant change in the levels of protein (sublethal concentration of 60% of 48h LC50 after 96h) gonadal, nervous and foot tissues are 48.1, 12.1 and 14.5%, respectively, amino acid are 273, 234 and 252%, respectively, DNA are 25.1, 38.9 and 42.1%, respectively and RNA are 12.2, 30.7 and 30.5%, respectively. These changes were time and concentration-dependent. In the withdrawal experiment, the snails were treated for 96h to transfer in freshwater for 7 d, which caused significant recovery in all the biochemical parameters. Conclusion: The present study concluded that the high molluscicidal activity of deltamethrin+MGK-264 simultaneous decrease in the levels of proteins, DNA, RNA and increase in the level of amino acids.

Biochemical Alterations Due to dimethoate in fresh water fish, Channa punctatus

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
K.T. Paithane ◽  
R.Y. Bhandare ◽  
S.E. Shinde ◽  
P.R. More ◽  
T.S. Pathan
Keyword(s):  
Fresh Water ◽  
Biochemical Parameters ◽  
Channa Punctatus ◽  
Fresh Water Fish ◽  
Biochemical Alterations ◽  
Protein Levels ◽  
Estimated Parameters ◽  
Significant Difference ◽  
Water Fish

Activity of a few biomarkers have been investigated on fresh water fish, Channa punctatus exposed to three sub – lethal concentrations of dimethoate(1/5, 1/10 and 1/15 of 96hrs LC50 values). The alteration in protein contents of liver, gill and muscle were investigated. The protein levels were found to be depleted in all the tissues after exposure to dimethoate over the control.All the organs showed the significant difference between control and exposed groups in all the estimated parameters on long exposure. The present study considers biochemical parameters as important biomarkers in determining the level of toxicity caused by the pesticide Dimethoate. Therefore the detailed results and observations are summarized in the present investigations.

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