scholarly journals EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF MACROALGAE COLLECTED FROM BAJA CALIFORNIA SUR, MEXICO

2021 ◽  
pp. 81-88
Author(s):  
ELENA BERENICE GARCÍA-LÓPEZ ◽  
MAURICIO MUÑOZ-OCHOA ◽  
CLAUDIA J. HERNÁNDEZ-GUERRERO ◽  
ANTONIO NIETO-CAMACHO ◽  
CHRISTINE J. BAND-SCHMIDT
Keyword(s):  
Baja California ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Baja California Sur ◽  
Gracilaria Vermiculophylla ◽  
Anti Inflammatory ◽  
Membrane Protection

Objective: This study aimed to establish the anti-inflammatory potential of macroalgae from Baja California Sur, Mexico. Methods: The anti-inflammatory activity of the extracts from 10 macroalgae species was evaluated in vitro and in vivo. Dichloromethane, ethanolic, and aqueous extracts were obtained from each species. The criteria used to determine anti-inflammatory activity included 1) the membrane protection of human red blood cells (HRBCs) against hemolysis induced by hypotonicity, 2) the inhibition of heat-induced protein denaturation (IDP), and 3) the inhibition of mouse ear edema and the myeloperoxidase (MPO) enzyme. Results: The dichloromethane extracts of Gracilaria vermiculophylla and Opuntiella californica were the most active, both in vitro and in vivo. These extracts resulted in values of HRBC membrane protection above 95%, which were similar to those obtained with diclofenac sodium. And reduce the inflammation with edema inhibition percentage up to 60 % while the control indomethacin was able to inhibit edema by 82.3 %. Conclusion: The bioprospection of several macroalgae species from Bahía de La Paz, Mexico, allowed the identification of two species with promising anti-inflammatory activity. Gracilaria vermiculophylla showed interesting topical anti-inflammatory effects and a profile of compounds (flavonoids and terpenes) that may contribute to the potential biological properties of this seaweed. This study represents the first report of the anti-inflammatory potential of O. californica.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF CURCUMIN AND CAPSAICIN AUGMENTED IN COMBINATION

2017 ◽  
Vol 9 (6) ◽  
pp. 145
Author(s):  
Thriveni Vasanth Kumar ◽  
Manjunatha H. ◽  
Rajesh Kp
Keyword(s):  
Acetic Acid ◽  
Protective Effect ◽  
Vascular Permeability ◽  
Diclofenac Sodium ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
The Individual

Objective: Dietary curcumin and capsaicin are well known for their health beneficial potencies. The current study was done to assess the anti-inflammatory activity of curcumin, capsaicin and their combination by employing in vitro and in vivo models.Methods: We investigated the protective effect of curcumin, capsaicin and their combination using in vitro heat induced human red blood cell (HRBC) membrane stabilisation, in vivo 3% agar induced leukocyte mobilisation and acetic acid induced vascular permeability assay.Results: Curcumin, capsaicin and their combination exhibited concentration dependent protective effect against heat-induced HRBC membrane destabilisation, while combined curcumin and capsaicin restored 87.0±0.64 % membrane stability and it is found to be better than curcumin, capsaicin and diclofenac sodium (75.0±0.25. 72±0.9 and 80.0±0.31 %) protective effect. In agar suspension induced leukocyte mobilization assay, the combined curcumin and capsaicin had shown 39.5±1.58 % of inhibition compared to individual curcumin and capsaicin, which showed moderate inhibition of 16.0±3.14 and 21.6±2.17 % respectively. Besides, the combined curcumin and capsaicin had shown highly significant inhibition of acetic acid-induced vascular permeability in rats (62.0±3.14 %), whereas individual curcumin and capsaicin showed moderate inhibition of vascular permeability with 36.0±2.41 and 43.0±1.92 % respectively.Conclusion: This study demonstrates the significant anti-inflammatory property of combined curcumin and capsaicin at half of the individual concentration of curcumin and capsaicin.

scholarly journals Suppression of inflammatory mediators by aqueous leaf extract of Crotalaria verrucosa: in vivo and in vitro analysis

2020 ◽  
Vol 9 (12) ◽  
pp. 1897
Author(s):  
Mohammad Mustakim Billah ◽  
Abir Huzaifa ◽  
M. Abdul Kader Khan ◽  
Nusrat Jahan Vabna ◽  
Kashfia Nawrin ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Medicinal Value ◽  
Aqueous Leaf Extract ◽  
Anti Inflammatory ◽  
Vitro Analysis ◽  
In Vitro Analysis

Background: Crotalaria verrucosa is a traditional plant frequently prescribed by the tribes for its medicinal value against inflammation. The present study was designed to investigate the scientific basis for medicinal value in inflammation by in vivo and in vitro analysis.Methods: Anti-inflammatory activity of the plant’s leaf was evaluated by two in vivo methods - carrageenan induced rat paw edema and xylene induced mice ear edema. Moreover, in vitro analysis was performed through heat induced hemolysis and heat induced protein denaturation methods.Results: The inflammation produced by carrageenan and xylene were effectively suppressed by the aqueous leaf extract of C. verrucosa (CVAQ) at 600 mg/kg body weight which was comparable to the standards. In heat induced hemolysis test the extract was able to inhibit the lysis up to 70% at 500 µg/ml whereas in heat induced protein denaturation test it reduces the percentage till 69% at the same concentration.Conclusions: The findings suggested that CVAQ possess moderate to high anti-inflammatory activity when applied in low to high concentrated doses. However, the study can only conclude from this basic evaluation that the extract needs to be further investigated for identifying the potential compound which contributed to such medicinal value of the plant.

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT AND DIFFERENT FRACTIONS OF CENTELLA ASIATICA LEAVES BY PROTEIN DENATURATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 86-89
Author(s):  
S Sharma ◽  
◽  
R. Trivedi ◽  
N. K. Choudhary
Keyword(s):  
Petroleum Ether ◽  
Skin Diseases ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Inflammation might be a complex organic reaction to a hazardous stimulant such as pathogens, or injured tissues and mainly causes itching, swelling, skin redness, warm and slight pain. Herbal drugs are widespread in India for their effectiveness, easy availability at low cost and provide low toxicity as compared to modern drugs. Centella asiatica is one of the oldest Ayurvedic medicinal plants, used in treatment of various skin diseases. The aim of our present research was to evaluate the in vitro anti-inflammatory activity of methanolic extract and different fractions of C. asiatica leaves. In protein denaturation method, the percentage inhibition for methanolic extract was observed to be 40.22%. The petroleum ether and n-butanol fraction of methanolic extract of C. asiatica were observed to exhibits 54.12 and 44.42% inhibition, respectively. Diclofenac sodium was used as a standard drug. In comparison with other fractions petroleum ether and n-butanol fractions showed best activity. The preliminary phytochemical studies of n-butanol fractions and n-butanol fractions showed the presence of terpenoids, flavonoids etc., which are used in the treatment of inflammation. Thus, we can call the latter as intense anti-inflammatory agent.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF NEURACANTHUS SPHAEROSTACHYUS DALZ. LEAVES

2019 ◽  
pp. 227-229
Author(s):  
DANGAR DK ◽  
PATEL NJ
Keyword(s):  
Vascular Permeability ◽  
Skin Diseases ◽  
Protein Denaturation ◽  
Scientific Evidence ◽  
Inflammatory Activity ◽  
Screening Methods ◽  
Paw Edema ◽  
Anti Inflammatory

Objective: Neuracanthus sphaerostachyus has been traditionally used to treat skin diseases, cough, and asthma. Lack of sufficient scientific evidence indicating the utility of this plant in the treatment of inflammation prompted us to investigate the anti-inflammatory activity of the plant in different experimental screening methods. Methods: In vitro and in vivo anti-inflammatory activity of the methanolic and aqueous extracts of N. sphaerostachyus (MENS and AENS) leaves at doses of 125, 250, and 500 mg/kg was evaluated with albumin denaturation and carrageenan-induced paw edema in rats and acetic acid-induced increased vascular permeability in mice. Results: Methanolic and aqueous extract significantly inhibited protein denaturation as well as edema induced by carrageenan and vascular permeability in mice dose dependently. Aspirin (0.1 mg/ml), indomethacin (10 mg/kg), and dexamethasone (5 mg/kg) were used as a standard control. Conclusion: It is concluded that MENS and AENS leaves exhibited significant anti-inflammatory activity.

scholarly journals Anti-inflammatory potential of african leaf water extract

2020 ◽  
Vol 1 (2) ◽  
pp. 46-53
Author(s):  
Lusi Agus Setiani ◽  
Zaldi Rusli
Keyword(s):  
Leaf Extract ◽  
Protein Denaturation ◽  
Scientific Information ◽  
Water Extract ◽  
Inflammatory Activity ◽  
In Vivo Test ◽  
Flavonoid Compounds ◽  
Anti Inflammatory

Inflammation is the immune system's main response to infection and irritation. African leaf (Gymnanthemum amygdalinum) is one of the medicinal plants that can be used as anti-inflammatory because of the presence of flavonoid compounds. Flavonoid compounds have an anti-inflammatory effect that can regulate arachidonic acid metabo-lism by inhibiting cyclooxygenase (COX) and lipooksigenase activi-ties. This study aims to obtain scientific information and the potential of African leaf extract as an anti-inflammatory in vitro and in vivo test. In this study, in vitro and in vivo anti-inflammatory activity tests were carried out. The in vitro anti-inflammatory activity was evaluated by the ability of African leaf extracts to prevent protein denaturation and in vivo anti-inflammatory activity by observing the reduction of edema in the soles of rats induced by carrageenan by giving three lev-els of doses of African leaf extract. The results showed that the African leaf extract at a concentration of 8273.91 mg L-1 was able to inhibit 50% denatured protein (IC50), while in the in vivo test African leaf had the potential as an anti-inflammatory with the most effective percent-age of inhibition at a dose of 200 mg kg-1 which is 85.20%. African leaf have the potential of approximately 2 times greater than the positive control of diclofenac sodium which is 45.70%.

scholarly journals In vitro and In vivo Anti-inflammatory Activity of the Extracts of Whole Plant Argyreia imbricata (Roth) Sant. & Patel

2020 ◽  
Vol 11 (4) ◽  
pp. 7317-7322
Author(s):  
Sebastin V ◽  
Gopalakrishnan G ◽  
Sreejith M ◽  
Anoob Kumar K I
Keyword(s):  
Ethyl Acetate ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Significant Activity ◽  
In Vivo Evaluation ◽  
Whole Plant ◽  
Anti Inflammatory

Plants of the genus Argyreia have ethnomedicinal importance, and several pharmacological activities are also reported. In this study, the anti-inflammatory activity of different extracts of Argyreia imbricata was evaluated by in vitro and in vivo methods. In both evaluations, standard, Diclofenac sodium was used for comparative evaluation. In this study, extraction of powdered whole plant material was done with different solvents viz., petroleum ether, chloroform, ethyl acetate and methanol by soxhelation. In vitro anti-inflammatory activity of all the prepared extracts was evaluated by stabilization of human red blood cell (HRBC) membrane in different temperature and tonicity conditions. Among the six different concentrations of four tested extracts, the ethyl acetate and methanol extracts (1000μg/ml) showed significant activity in the in vitro evaluation. They were selected for the in vivo evaluation on the paw oedema induced by carrageenan on Wistar albino rats. Two doses, 200mg.kg-1 and 400mg.kg-1 of the test extracts were subjected to evaluation. Both the tested extracts showed the activity, particularly, the methanol extract in the dose of 400mg.kg-1 showed significant activity. Results of this study strongly supported the anti-inflammatory activity of the tested extracts. Further, studies on toxicity, identification, isolation of the active constituents may give useful results.

scholarly journals Assessment of In vitro Anti-inflammatory Activity of Ginger and Diclofenac sodium combination

2020 ◽  
pp. 442-447
Author(s):  
Mousmi D. Thakur ◽  
Navin R. Sheth ◽  
Mihir K. Raval
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Research Work ◽  
Pharmacological Action ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Ginger Extract ◽  
Anti Inflammatory

The present research work aimed at evaluating the anti-inflammatory activity of Zingiber officinalis with Diclofenac sodium by HRBC membrane stabilization & Protein denaturation. The precluding of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization at different concentrations was performed for methanolic, hydro-methanolic ginger extract and diclofenac sodium. At a dose of 50µg/ml the maximum membrane stabilization 86.34% was found for Ginger extract(test) and at a dose of 500 mcg/ml membrane stabilization was found 91.16% for diclofenac sodium(standard) and the membrane stabilization for combination (ginger with diclofenac sodium) at a dose of 50µg/ml was recorded 86.43%, as the concentration increase(1000 mcg/ml) for combination(ginger with diclofenac sodium) the percentage protection was decreased. In vitro protein denaturation was performed by using egg albumin method. Maximum inhibition was observed in case of methanolic extract of ginger at concentration 1000mcg/ml and it was 78.83±5.17 and in hydro methanolic extract for Diclofenac sodium at concentration 1000mcg/ml and it was 63.37±2.78.Minimum inhibition observed in combination of methanolic extract of ginger and diclofenac sodium at concentration 1000mcg/ml and it was 25.27±1.76 and in combination of hydro-methanolic extract of ginger and Diclofenac sodium at concentration 1000mcg/ml and it was 28.23±3.14. The results of this study divulge that low dose combination of ginger and diclofenac sodium has higher anti-inflammatory activity than diclofenac sodium and ginger alone. With this initial study, research work could be extended further; therefore, the particular pharmacological action for the combination of ginger with diclofenac sodium could be discovered.

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF SYRINGIC ACID

2018 ◽  
pp. 71-73 ◽  
Author(s):  
Shilpee Chanda ◽  
Archana R. Juvekar
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Syringic Acid ◽  
Inflammatory Activity ◽  
Proteinase Activity ◽  
Membrane Stabilization ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Proteinase Inhibition

Objective: The present study was carried out to investigate the in vitro anti-inflammatory activity of syringic acid (SA).  Methods: SA was tested for it's in vitro anti-inflammatory activity at different concentrations in protein denaturation, proteinase inhibition and human red blood cell (HRBC) membrane stabilization assay. The reference drugs used were aspirin and diclofenac sodium. Results: SA showed concentration-dependent inhibition of protein denaturation and proteinase activity with a half-maximal inhibitory concentration (IC50) value of 49.38±0.56 µg/ml and 53.73±0.27 µg/ml respectively. Heat-induced haemolysis was inhibited by SA with an IC50 value of 57.13±0.24 µg/ml. SA also inhibited the hypotonicity-induced haemolysis (IC50 value of 53.87±0.72 µg/ml). Conclusion: From the present study, we can conclude that SA possesses appreciable anti-inflammatory effect against denaturation of proteins, proteinase activity, and human red blood membrane stabilization assays. Further studies are required for determining the possible mechanisms behind its anti-inflammatory action.

scholarly journals Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Godswill Nduka Anyasor ◽  
Azeezat Adenike Okanlawon ◽  
Babafemi Ogunbiyi
Keyword(s):  
Erythrocyte Membrane ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Paw Edema ◽  
In Vivo Models ◽  
In Vivo Inflammation ◽  
Anti Inflammatory

Abstract Background Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J. secunda leaves (MEJSL) using in vitro and in vivo inflammation models. Methods Seventy-percent MEJSL was prepared following standard procedure. In vitro anti-inflammatory assays were performed using heat-induced bovine serum albumin (BSA) denaturation and erythrocyte membrane stabilization assays. Carrageenan and formaldehyde induced inflammation in rat models were used to evaluate the anti-inflammatory activity of MEJSL in vivo. Diclofenac sodium was used as a reference drug. In addition, liver and kidney function assays and hematological analysis were carried out. Results Data revealed that varying concentrations of MEJSL significantly (P < 0.05) inhibited heat-induced BSA denaturation and stabilized erythrocyte membrane against hypotonicity-induced hemolysis when compared with diclofenac sodium in a concentration-dependent manner. In vivo study showed that 10 mg/kg body weight (b.w.) diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL suppressed carrageenan-induced paw edema at the sixth hour by 71.14%, 83.08%, and 89.05%, respectively. Furthermore, 10 mg/kg b.w. diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL inhibited formaldehyde-induced paw edema by 72.53%, 74.73%, and 76.48%, respectively. Animals treated with varying doses of MEJSL had reduced plasma aspartate aminotransferase and alanine aminotransferase activities; urea and creatinine concentrations; and modulated hematological parameters when compared with the untreated control group. Conclusions Findings from this study showed that MEJSL exhibited substantial anti-inflammatory actions in the in vitro and in vivo models. It also indicated that MEJSL anti-inflammatory mechanisms of action could be through interference with phase 2 inflammatory stressors, upregulation of cytoprotective genes, stabilization of inflammatory cell membranes and immunomodulatory activity.

scholarly journals EVALUATION OF IN VITRO ANTIDIABETIC AND ANTI-INFLAMMATORY ACTIVITIES OF LEAVES EXTRACT OF BOEHMERIA RUGULOSA

2018 ◽  
Vol 11 (9) ◽  
pp. 200
Author(s):  
Abha Shukla ◽  
Anchal Choudhary
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Standard Drug ◽  
Successive Extraction ◽  
Ic50 Value ◽  
Anti Inflammatory

Objective: The objective of the study is to evaluate in vitro antidiabetic and anti-inflammatory activity of different extracts of leaves of Boehmeria rugulosa by different methods.Methods: In vitro α-glucose and α-amylase were used for antidiabetic activity and lipoxygenase, and protein denaturation method of inhibition assays was used to measure anti-inflammatory activity. Successive extraction of leaves petroleum ether (PE), chloroform (CH), ethyl acetate (EA), acetone (AC), and ethanol (ETH) was performed, and extracts obtained from the extraction were applicable to these activities.Results: The AC extract of leaves shows significantly in vitro antidiabetic activity, and AC has offered significant result 470.07±0.65 μg/mL in the inhibition of α-glucosidase and also for α-amylase assay 698.15±1.71 μg/mL. Acarbose was used as standard. In lipoxidase method, AC had shown better results and in protein denaturation method EA shown the higher inhibition (78.06±0.5 μg/ml) than the other extracts. The standard drug diclofenac sodium also offered significant inhibition against lipoxidase enzyme method with IC50 value 21.76±1.29 μg/mL.Conclusion: These findings suggest that the AC and EA possess potent antidiabetic and anti-inflammatory activities in vitro conditions.

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