ANTI-INFLAMMATORY ACTIVITY OF CURCUMIN AND CAPSAICIN AUGMENTED IN COMBINATION

Objective: Dietary curcumin and capsaicin are well known for their health beneficial potencies. The current study was done to assess the anti-inflammatory activity of curcumin, capsaicin and their combination by employing in vitro and in vivo models.Methods: We investigated the protective effect of curcumin, capsaicin and their combination using in vitro heat induced human red blood cell (HRBC) membrane stabilisation, in vivo 3% agar induced leukocyte mobilisation and acetic acid induced vascular permeability assay.Results: Curcumin, capsaicin and their combination exhibited concentration dependent protective effect against heat-induced HRBC membrane destabilisation, while combined curcumin and capsaicin restored 87.0±0.64 % membrane stability and it is found to be better than curcumin, capsaicin and diclofenac sodium (75.0±0.25. 72±0.9 and 80.0±0.31 %) protective effect. In agar suspension induced leukocyte mobilization assay, the combined curcumin and capsaicin had shown 39.5±1.58 % of inhibition compared to individual curcumin and capsaicin, which showed moderate inhibition of 16.0±3.14 and 21.6±2.17 % respectively. Besides, the combined curcumin and capsaicin had shown highly significant inhibition of acetic acid-induced vascular permeability in rats (62.0±3.14 %), whereas individual curcumin and capsaicin showed moderate inhibition of vascular permeability with 36.0±2.41 and 43.0±1.92 % respectively.Conclusion: This study demonstrates the significant anti-inflammatory property of combined curcumin and capsaicin at half of the individual concentration of curcumin and capsaicin.

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ANTI-INFLAMMATORY ACTIVITY OF NEURACANTHUS SPHAEROSTACHYUS DALZ. LEAVES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i3.30059 ◽

2019 ◽

pp. 227-229

Author(s):

DANGAR DK ◽

PATEL NJ

Keyword(s):

Vascular Permeability ◽

Skin Diseases ◽

Protein Denaturation ◽

Scientific Evidence ◽

Inflammatory Activity ◽

Screening Methods ◽

Paw Edema ◽

Anti Inflammatory

Objective: Neuracanthus sphaerostachyus has been traditionally used to treat skin diseases, cough, and asthma. Lack of sufficient scientific evidence indicating the utility of this plant in the treatment of inflammation prompted us to investigate the anti-inflammatory activity of the plant in different experimental screening methods. Methods: In vitro and in vivo anti-inflammatory activity of the methanolic and aqueous extracts of N. sphaerostachyus (MENS and AENS) leaves at doses of 125, 250, and 500 mg/kg was evaluated with albumin denaturation and carrageenan-induced paw edema in rats and acetic acid-induced increased vascular permeability in mice. Results: Methanolic and aqueous extract significantly inhibited protein denaturation as well as edema induced by carrageenan and vascular permeability in mice dose dependently. Aspirin (0.1 mg/ml), indomethacin (10 mg/kg), and dexamethasone (5 mg/kg) were used as a standard control. Conclusion: It is concluded that MENS and AENS leaves exhibited significant anti-inflammatory activity.

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In vitro and In vivo Anti-inflammatory Activity of the Extracts of Whole Plant Argyreia imbricata (Roth) Sant. & Patel

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3872 ◽

2020 ◽

Vol 11 (4) ◽

pp. 7317-7322

Author(s):

Sebastin V ◽

Gopalakrishnan G ◽

Sreejith M ◽

Anoob Kumar K I

Keyword(s):

Ethyl Acetate ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

Albino Rats ◽

Significant Activity ◽

In Vivo Evaluation ◽

Whole Plant ◽

Anti Inflammatory

Plants of the genus Argyreia have ethnomedicinal importance, and several pharmacological activities are also reported. In this study, the anti-inflammatory activity of different extracts of Argyreia imbricata was evaluated by in vitro and in vivo methods. In both evaluations, standard, Diclofenac sodium was used for comparative evaluation. In this study, extraction of powdered whole plant material was done with different solvents viz., petroleum ether, chloroform, ethyl acetate and methanol by soxhelation. In vitro anti-inflammatory activity of all the prepared extracts was evaluated by stabilization of human red blood cell (HRBC) membrane in different temperature and tonicity conditions. Among the six different concentrations of four tested extracts, the ethyl acetate and methanol extracts (1000μg/ml) showed significant activity in the in vitro evaluation. They were selected for the in vivo evaluation on the paw oedema induced by carrageenan on Wistar albino rats. Two doses, 200mg.kg-1 and 400mg.kg-1 of the test extracts were subjected to evaluation. Both the tested extracts showed the activity, particularly, the methanol extract in the dose of 400mg.kg-1 showed significant activity. Results of this study strongly supported the anti-inflammatory activity of the tested extracts. Further, studies on toxicity, identification, isolation of the active constituents may give useful results.

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Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models

Clinical Phytoscience ◽

10.1186/s40816-019-0137-8 ◽

2019 ◽

Vol 5 (1) ◽

Cited By ~ 1

Author(s):

Godswill Nduka Anyasor ◽

Azeezat Adenike Okanlawon ◽

Babafemi Ogunbiyi

Keyword(s):

Erythrocyte Membrane ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

Dependent Manner ◽

Paw Edema ◽

In Vivo Models ◽

In Vivo Inflammation ◽

Anti Inflammatory

Abstract Background Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J. secunda leaves (MEJSL) using in vitro and in vivo inflammation models. Methods Seventy-percent MEJSL was prepared following standard procedure. In vitro anti-inflammatory assays were performed using heat-induced bovine serum albumin (BSA) denaturation and erythrocyte membrane stabilization assays. Carrageenan and formaldehyde induced inflammation in rat models were used to evaluate the anti-inflammatory activity of MEJSL in vivo. Diclofenac sodium was used as a reference drug. In addition, liver and kidney function assays and hematological analysis were carried out. Results Data revealed that varying concentrations of MEJSL significantly (P < 0.05) inhibited heat-induced BSA denaturation and stabilized erythrocyte membrane against hypotonicity-induced hemolysis when compared with diclofenac sodium in a concentration-dependent manner. In vivo study showed that 10 mg/kg body weight (b.w.) diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL suppressed carrageenan-induced paw edema at the sixth hour by 71.14%, 83.08%, and 89.05%, respectively. Furthermore, 10 mg/kg b.w. diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL inhibited formaldehyde-induced paw edema by 72.53%, 74.73%, and 76.48%, respectively. Animals treated with varying doses of MEJSL had reduced plasma aspartate aminotransferase and alanine aminotransferase activities; urea and creatinine concentrations; and modulated hematological parameters when compared with the untreated control group. Conclusions Findings from this study showed that MEJSL exhibited substantial anti-inflammatory actions in the in vitro and in vivo models. It also indicated that MEJSL anti-inflammatory mechanisms of action could be through interference with phase 2 inflammatory stressors, upregulation of cytoprotective genes, stabilization of inflammatory cell membranes and immunomodulatory activity.

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In vivo neuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigarna longifolia Roxb. bark extract

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0102 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 4

Author(s):

Md. Zia Uddin ◽

Md. Sohel Rana ◽

Shahadat Hossain ◽

Sumaiya Ferdous ◽

Emon Dutta ◽

...

Keyword(s):

Acetic Acid ◽

Free Radical ◽

Methanol Extract ◽

Diclofenac Sodium ◽

Neuroprotective Activity ◽

Clot Lysis ◽

Sleeping Time ◽

Anti Inflammatory

AbstractBackgroundThe study investigated the in vivo neuroprotective, antinociceptive, anti-inflammatory potential and in vitro antioxidant and clot lysis activities of crude methanol extract with its different solvent-soluble fractions like petroleum ether (PESF), carbon tetrachloride (CTSF), chloroform (CSF) and aqueous (AQSF) of Holigarna longifolia Roxb.MethodsPhenobarbitone-induced sleeping time method was used for the neuroprotective activity, writhing response experimental model introduced by acetic acid was designed for antinociceptive efficacy, carrageenan-induced paw edema model was carried out for anti-inflammatory activity, DPPH free radical scavenging activity was assessed for antioxidant activity and clot lysis model was investigated for the thrombolytic potential of the plant.ResultsOn investigation it was found that methanol extract and CS fraction revealed statistically meaningful (p<0.05) neuroprotective activity by increasing phenobarbitone-induced sleeping time of mice, produced substantial (p<0.05) inflammation inhibitory efficacy compared to standard diclofenac sodium and also exhibited statistically significant (p<0.01) oxidative stress inhibitory efficacy by inhibiting free radical formation compared to ascorbic acid as standard. Only methanol extract produced significant (p<0.05) antinociceptive activity by inhibiting abdominal writhes produced by acetic acid compared to standard analgesic drug diclofenac sodium. And only aqueous soluble fraction exhibited moderate clot lysis activity compared to streptokinase as standard.ConclusionThe findings demonstrate that H. longifolia could be potential neuroprotective due to its justified antioxidative capacity as well as clot lysis properties.

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EVALUATION OF IN VITRO ANTIDIABETIC AND ANTI-INFLAMMATORY ACTIVITIES OF LEAVES EXTRACT OF BOEHMERIA RUGULOSA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.25906 ◽

2018 ◽

Vol 11 (9) ◽

pp. 200

Author(s):

Abha Shukla ◽

Anchal Choudhary

Keyword(s):

Protein Denaturation ◽

Diclofenac Sodium ◽

Significant Inhibition ◽

Inflammatory Activity ◽

Antidiabetic Activity ◽

Standard Drug ◽

Successive Extraction ◽

Ic50 Value ◽

Anti Inflammatory

Objective: The objective of the study is to evaluate in vitro antidiabetic and anti-inflammatory activity of different extracts of leaves of Boehmeria rugulosa by different methods.Methods: In vitro α-glucose and α-amylase were used for antidiabetic activity and lipoxygenase, and protein denaturation method of inhibition assays was used to measure anti-inflammatory activity. Successive extraction of leaves petroleum ether (PE), chloroform (CH), ethyl acetate (EA), acetone (AC), and ethanol (ETH) was performed, and extracts obtained from the extraction were applicable to these activities.Results: The AC extract of leaves shows significantly in vitro antidiabetic activity, and AC has offered significant result 470.07±0.65 μg/mL in the inhibition of α-glucosidase and also for α-amylase assay 698.15±1.71 μg/mL. Acarbose was used as standard. In lipoxidase method, AC had shown better results and in protein denaturation method EA shown the higher inhibition (78.06±0.5 μg/ml) than the other extracts. The standard drug diclofenac sodium also offered significant inhibition against lipoxidase enzyme method with IC50 value 21.76±1.29 μg/mL.Conclusion: These findings suggest that the AC and EA possess potent antidiabetic and anti-inflammatory activities in vitro conditions.

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EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF MACROALGAE COLLECTED FROM BAJA CALIFORNIA SUR, MEXICO

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2021v13i8.41453 ◽

2021 ◽

pp. 81-88

Author(s):

ELENA BERENICE GARCÍA-LÓPEZ ◽

MAURICIO MUÑOZ-OCHOA ◽

CLAUDIA J. HERNÁNDEZ-GUERRERO ◽

ANTONIO NIETO-CAMACHO ◽

CHRISTINE J. BAND-SCHMIDT

Keyword(s):

Baja California ◽

Protein Denaturation ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

Baja California Sur ◽

Gracilaria Vermiculophylla ◽

Anti Inflammatory ◽

Membrane Protection

Objective: This study aimed to establish the anti-inflammatory potential of macroalgae from Baja California Sur, Mexico. Methods: The anti-inflammatory activity of the extracts from 10 macroalgae species was evaluated in vitro and in vivo. Dichloromethane, ethanolic, and aqueous extracts were obtained from each species. The criteria used to determine anti-inflammatory activity included 1) the membrane protection of human red blood cells (HRBCs) against hemolysis induced by hypotonicity, 2) the inhibition of heat-induced protein denaturation (IDP), and 3) the inhibition of mouse ear edema and the myeloperoxidase (MPO) enzyme. Results: The dichloromethane extracts of Gracilaria vermiculophylla and Opuntiella californica were the most active, both in vitro and in vivo. These extracts resulted in values of HRBC membrane protection above 95%, which were similar to those obtained with diclofenac sodium. And reduce the inflammation with edema inhibition percentage up to 60 % while the control indomethacin was able to inhibit edema by 82.3 %. Conclusion: The bioprospection of several macroalgae species from Bahía de La Paz, Mexico, allowed the identification of two species with promising anti-inflammatory activity. Gracilaria vermiculophylla showed interesting topical anti-inflammatory effects and a profile of compounds (flavonoids and terpenes) that may contribute to the potential biological properties of this seaweed. This study represents the first report of the anti-inflammatory potential of O. californica.

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Evaluation of anti-inflammatory activity and anti-oxidant potential of portulaca quadrifida linn.

Journal of Innovations in Applied Pharmaceutical Science (JIAPS) ◽

10.37022/jiaps.v6i3.255 ◽

2021 ◽

pp. 58-65

Author(s):

Anil Kumar Y ◽

Abdul Mahaboob Subhani Sk. ◽

Ravi Kumar Konda ◽

Prathyusha A

Keyword(s):

Acetic Acid ◽

Vascular Permeability ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

Permeability Model ◽

Anti Oxidant ◽

Anti Inflammatory ◽

Leading Compounds

Portulaca quadrifidaL. (PQ) having the phytochemicals like Alkaloids, flavonoids, Saponins, tannins, glycosides, carbohydrates, aminoacids, triterpenoids. The present study was performed to evaluate the antioxidant and anti-inflammatory activities by using acetic acid induced vascular permeability model in mice & acetic acid induced colitis in rats significantly. PQ (100 mg/kg, p.o.) presented a significant anti-inflammatory activity towards acetic acid induced vascular permeability model in mice in comparison to Diclofenac sodium(10 mg/kg, s.c.) and acetic acid induced colitis in rats in comparison to 5-ASA. Our findings suggest that, PQ contains potential antioxidant and anti-inflammatory compounds which will aid us to conduct bioactivity guided isolation & characterization of leading compounds in due course.

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