Nanoceria, alone or in combination with cigarette-smoke condensate, induce transforming and epigenetic cancer-like features in vitro

Nanomedicine ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 293-305
Author(s):  
Sandra Ballesteros ◽  
Irene Barguilla ◽  
Ricard Marcos ◽  
Alba Hernández
Keyword(s):  
Cigarette Smoke ◽  
Cell Transformation ◽  
Cigarette Smoke Condensate ◽  
Positive Interaction ◽  
Bronchial Epithelial ◽  
Cell Transforming ◽  
Potential Interactions ◽  
Transcriptome Level

Aim: To detect cell transformation effects of nanoceria after long-term exposure (up to 6 weeks) and to determine their potential interactions with cigarette smoke condensate, as a model of environmental carcinogenic pollutant. Materials & methods: Human bronchial epithelial BEAS-2 cells were used to determine transformation effects (invasion and tumorspheres induction), as well as changes in the expression of a battery of miRNAs related to the carcinogenesis process. Results: Nanoceria- and co-exposed cells exhibit cell transforming potential, with significantly increased invasion and tumorsphere formation abilities. Likewise, these exposures produced a high impact on the battery of miRNAs used. Conclusion: Nanoceria exposure induces cell-transformation and shows a positive interaction with the cell-transforming effects of cigarette smoke condensate. Besides, cerium dioxide nanoparticles and the co-exposure produced potential toxicity at the transcriptome level, which is related to tumorigenesis.

scholarly journals Gene Expression in Normal Human Bronchial Epithelial (NHBE) Cells Following In Vitro Exposure to Cigarette Smoke Condensate

2005 ◽  
Vol 86 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Wanda R. Fields ◽  
Randi M. Leonard ◽  
Pamela S. Odom ◽  
Brian K. Nordskog ◽  
Michael W. Ogden ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cigarette Smoke ◽  
Cigarette Smoke Condensate ◽  
Normal Human Bronchial Epithelial ◽  
Bronchial Epithelial ◽  
In Vitro Exposure ◽  
Normal Human

scholarly journals Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione

2005 ◽  
Vol 6 (1) ◽  
Author(s):  
Fabrizio Luppi ◽  
Jamil Aarbiou ◽  
Sandra van Wetering ◽  
Irfan Rahman ◽  
Willem I de Boer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Wound Closure ◽  
Bronchial Epithelial Cells ◽  
Cigarette Smoke Condensate ◽  
Bronchial Epithelial

scholarly journals Synthesis and in vitro characterization of the genotoxic, mutagenic and cell-transforming potential of nitrosylated heme

2020 ◽  
Vol 94 (11) ◽  
pp. 3911-3927 ◽  
Author(s):  
Tina Kostka ◽  
Jörg Fohrer ◽  
Claudia Guigas ◽  
Karlis Briviba ◽  
Nina Seiwert ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Malignant Cell ◽  
Red Meat ◽  
Colorectal Carcinogenesis ◽  
In Vivo Studies ◽  
Processed Meat ◽  
Cell Transforming ◽  
Malignant Cell Transformation

Abstract Data from epidemiological studies suggest that consumption of red and processed meat is a factor contributing to colorectal carcinogenesis. Red meat contains high amounts of heme, which in turn can be converted to its nitrosylated form, NO-heme, when adding nitrite-containing curing salt to meat. NO-heme might contribute to colorectal cancer formation by causing gene mutations and could thereby be responsible for the association of (processed) red meat consumption with intestinal cancer. Up to now, neither in vitro nor in vivo studies characterizing the mutagenic and cell transforming potential of NO-heme have been published due to the fact that the pure compound is not readily available. Therefore, in the present study, an already existing synthesis protocol was modified to yield, for the first time, purified NO-heme. Thereafter, newly synthesized NO-heme was chemically characterized and used in various in vitro approaches at dietary concentrations to determine whether it can lead to DNA damage and malignant cell transformation. While NO-heme led to a significant dose-dependent increase in the number of DNA strand breaks in the comet assay and was mutagenic in the HPRT assay, this compound tested negative in the Ames test and failed to induce malignant cell transformation in the BALB/c 3T3 cell transformation assay. Interestingly, the non-nitrosylated heme control showed similar effects, but was additionally able to induce malignant transformation in BALB/c 3T3 murine fibroblasts. Taken together, these results suggest that it is the heme molecule rather than the NO moiety which is involved in driving red meat-associated carcinogenesis.

scholarly journals Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium

2015 ◽  
Vol 87 (2) ◽  
pp. 997-1005 ◽  
Author(s):  
Jinqiang Hu ◽  
Tao Wei ◽  
Siwen Sun ◽  
Aijing Zhao ◽  
Chunping Xu
Keyword(s):  
Cigarette Smoke ◽  
Carbohydrate Composition ◽  
Cigarette Smoke Condensate ◽  
Essential Factor ◽  
Standard Strain ◽  
Acetic Acid Production ◽  
Bifidobacterium Animalis ◽  
Molecular Determination

The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium. Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC). The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC. The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production. Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination. By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition. The molecular weight mass of Fr-I changed from 3.33×105 g/mol (without CSC) to 2.99×105 (with CSC). In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.

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