scholarly journals Lettuce Production under Reduced Levels of N-fertilizer in the Presence of Plant Growth-promoting Bacillus spp. Bacteria

2019 ◽  
Vol 13 (4) ◽  
pp. 1941-1952
Author(s):  
Wilson Story Venancio ◽  
Juliana Marcolino Gomes ◽  
Andre Shigueyoshi Nakatani ◽  
Mariangela Hungria ◽  
Ricardo Silva Araujo
Keyword(s):  
Plant Growth ◽  
N Fertilizer ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Forage Mass, Tillering, Nutritive Value and Root System of Ruzigrass Inoculated with Plant Growth Promoting Bacteria Associated with Doses of N-Fertilizer

2020 ◽  
Vol 8 (10) ◽  
pp. 41-55
Author(s):  
Artur Roque Domingues Barreiros ◽  
Ulysses Cecato ◽  
Camila Fernandes Domingues Duarte ◽  
Mariangela Hungria ◽  
Thiago Trento Biserra ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Nutritive Value ◽  
Block Design ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

The aim of this study was evaluating the effect of the inoculation of plant growth promoting bacteria (PGPB) in forage mass, tillering, nutritive value and root system of ruzigrass (Urochloa ruziziensis (R. Germ. & Evrard) Crins (syn. of Brachiaria ruziziensis) associated with doses of N-fertilizer. The bacteria inoculated were Azospirillum brasilense Ab-V5, Pseudomonas fluorescens CCTB03 and Pantoea ananatis AMG 521, plus the control treatment (non-inoculated), associated with doses of N-fertilizer (0, 50 and 100 kg N ha-1). The experiment was performed in a randomized block design, in a 4x3 factorial scheme, with four replicates, totaling 48 plots (12 m2). There were no effects of the PGPB and the use of N-fertilizer on the leaf blade, stem+sheath, forage mass, daily and yearly accumulation of forage mass. The PGPB did not have influence on the density of tillers. The doses of 50 and 100 kg of N ha-1 increased the amount of tillers. The AMG 521 strain associated with N-fertilizer provided heavier tillers. There was no effect of the PGPB on crude protein (CP), neutral detergent fiber (NDF), as well as acid detergent fiber (ADF), and in vitro digestibility of the dry matter (IVDDM).  The use of 100 kg of N ha-1 contributed to an increase in CP and a decrease in NDF. The AMG 521 strain contributed to a smaller diameter of the root. Strains CCTB03 and AMG 521 demonstrated a smaller area, length and root density when associated with the dose of 50kg of N ha-1. In general, the PGPB were not efficient in promoting productive increments in ruzigrass.

scholarly journals Bacterial Diversity and Community Structure in Typical Plant Rhizosphere

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 179 ◽  
Author(s):  
Alawiye ◽  
Babalola
Keyword(s):  
Plant Growth ◽  
Soil Health ◽  
Plant Growth Promoting Rhizobacteria ◽  
Vital Role ◽  
Success Story ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Microbe Interactions ◽  
Growth Promoting

Bacteria play a vital role in the quality of soil, health, and the production of plants. This has led to several studies in understanding the diversity and structure in the plant rhizosphere. Over the years, there have been overwhelming advances in molecular biology which have led to the development of omics techniques which utilize RNA, DNA, or proteins as biomolecules; these have been gainfully used in plant–microbe interactions. The bacterial community found in the rhizosphere is known for its colonization around the roots due to availability of nutrients, and composition, and it affects the plant growth directly or indirectly. Metabolic fingerprinting enables a snapshot of the metabolic composition at a given time. We review metabolites with ample information on their benefit to plants and which are found in rhizobacteria such as Pseudomonas spp. and Bacillus spp. Exploring plant-growth-promoting rhizobacteria using omics techniques can be a true success story for agricultural sustainability.

scholarly journals Biomass Yield, Nitrogen Content and Uptake, And Nutritive Value of Alfalfa Co-Inoculated with Plant-Growth Promoting Bacteria

2020 ◽  
Vol 8 (5) ◽  
pp. 400-420
Author(s):  
Cecilio Viega Soares Filho ◽  
Leonardo Aurélio Silva ◽  
Jaqueline Silva Boregio ◽  
Mariangela Hungria ◽  
Adônis Moreira ◽  
...  
Keyword(s):  
Plant Growth ◽  
Sinorhizobium Meliloti ◽  
Nutritive Value ◽  
Dry Weight ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
Plant Growth Promoting Bacteria ◽  
Total N ◽  
Plant Growth Promoting ◽  
Growth Promoting

Alfalfa (Medicago sativa L.) has high forage yield potential, protein quality, palatability, and digestibility, and low seasonality. The aim of this study was to evaluate the effects of strains of Sinorhizobium meliloti and Azospirillum brasilense on the nutritive content, bromatological composition (crude protein [CP], neutral detergent fiber [NDF], acid detergent fiber [ADF], and in vitro digestibility of dry weight [IVDDW]), and shoot dry weight (SDW), relative chlorophyll index (RCI), number of tillers (NT), plant height (PH), and root dry weight (RDW) and volume (RV), of alfalfa grown in a Typic Ultisol. The experiment consisted of eight combinations of plant-growth promoting bacteria (PGPB). The treatments were as follows: T1:non-inoculated control without N-fertilizer (NI); T2: NI + N-fertilizer, and inoculated with T3: Sinorhizobium (=Ensifer) meliloti SEMIA 116 + N-fertilizer; T4: S. meliloti SEMIA 116 + A. brasilense Ab-V5 + Ab-V6 + N-fertilizer; T5: S. meliloti SEMIA 134 + N-fertilizer; T6: S. meliloti SEMIA 134 + co-inoculation + N-fertilizer; T7: S. meliloti SEMIA 135 + N-fertilizer; and T8: S. meliloti SEMIA 135 + co-inoculation + N-fertilizer. S. meliloti strains are used in commercial inoculants for the alfalfa, and A. brasilense for several non-legumes and legumes in Brazil. The experiment was performed for three successive cuts under greenhouse conditions. Application of N-fertilization increases the production cost, making alfalfa cultivation unviable. Inoculation with three strains of Sinorhizobium meliloti highly promoted alfalfa growth, considering several parameters, including PH, RCI, NT, SDW and RDW, nutritive value, and with an emphasis on RV, and total N content and total N accumulated in shoots and roots. No further increases were observed with the co-inoculation with the PGPB A. brasilense. Studies in field and greenhouse conditions are necessary to verify the benefits of the use of PGPB in the cultivation of alfalfa.

scholarly journals Screening of Potent Arsenic Resistant and Plant Growth Promoting Bacillus species from the Soil of Terai Region of Nepal

2019 ◽  
Vol 6 ◽  
pp. 1-9
Author(s):  
Pramod Poudel ◽  
Ashish Nepal ◽  
Rashmi Roka Magar ◽  
Pratibha Rauniyar ◽  
Lil Buda Magar
Keyword(s):  
Plant Growth ◽  
Sodium Arsenite ◽  
16S Rrna Gene Sequencing ◽  
Bacillus Species ◽  
Rrna Gene ◽  
Sodium Arsenate ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing

Objectives: To isolate arsenic resistant Bacillus spp. and to determine plant growth promoting activities.  Methods: Eighteen soil samples were collected from the agricultural soil of Terai region of Nepal. Selective isolation of Bacillus species was done by heating the soil at 80 ºC for 15 minutes before the isolation. Nutrient agar was used as an isolation medium. Screening of arsenic resistant Bacillus species was done using nutrient agar supplemented with 100 ppm sodium arsenate and sodium arsenite. For plant growth promoting activity; IAA production was detected taking 0.1% tryptophane and measuring absorbance at 540 nm, NH3 production was tested by Nessler’s reagent and phosphate solubilization activity was detected by growing colonies on Pikovskaya’s agar. Sugar assimilation test was performed to identify the isolates. Most potent arsenic resistant isolate was identified by 16S rRNA gene sequencing. Results: Among 54 randomly selected isolates, 42 were found to be Gram-positive rod-shaped, spore-forming while 12 isolates were Gram-negative bacteria. The isolates IN12a, M12a and BG34a showed growth on 100 ppm sodium arsenite containing NA. Only isolate M12a tolerated up to 1000 ppm and 15000 ppm of sodium arsenite and sodium arsenate respectively, while other isolates could not grow above 400 ppm sodium arsenite. The isolates IN12a and M12a were able to produce IAA and solubilize phosphate while BG34a could not. Both the isolates IN12a and M12a were able to utilize the sugars glucose, fructose, lactose, sucrose, galactose, mannose, mannitol, maltose and xylose.  Based on the 16S rRNA gene sequencing, isolate M12a was identified to be Bacillus flexus with highest similarity of 99.2%. Conclusion: Arsenic resistant and plant growth promoting Bacillus spp. was isolated from the agricultural soil of Terai region of Nepal

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