scholarly journals Forage Mass, Tillering, Nutritive Value and Root System of Ruzigrass Inoculated with Plant Growth Promoting Bacteria Associated with Doses of N-Fertilizer

2020 ◽  
Vol 8 (10) ◽  
pp. 41-55
Author(s):  
Artur Roque Domingues Barreiros ◽  
Ulysses Cecato ◽  
Camila Fernandes Domingues Duarte ◽  
Mariangela Hungria ◽  
Thiago Trento Biserra ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Nutritive Value ◽  
Block Design ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

The aim of this study was evaluating the effect of the inoculation of plant growth promoting bacteria (PGPB) in forage mass, tillering, nutritive value and root system of ruzigrass (Urochloa ruziziensis (R. Germ. & Evrard) Crins (syn. of Brachiaria ruziziensis) associated with doses of N-fertilizer. The bacteria inoculated were Azospirillum brasilense Ab-V5, Pseudomonas fluorescens CCTB03 and Pantoea ananatis AMG 521, plus the control treatment (non-inoculated), associated with doses of N-fertilizer (0, 50 and 100 kg N ha-1). The experiment was performed in a randomized block design, in a 4x3 factorial scheme, with four replicates, totaling 48 plots (12 m2). There were no effects of the PGPB and the use of N-fertilizer on the leaf blade, stem+sheath, forage mass, daily and yearly accumulation of forage mass. The PGPB did not have influence on the density of tillers. The doses of 50 and 100 kg of N ha-1 increased the amount of tillers. The AMG 521 strain associated with N-fertilizer provided heavier tillers. There was no effect of the PGPB on crude protein (CP), neutral detergent fiber (NDF), as well as acid detergent fiber (ADF), and in vitro digestibility of the dry matter (IVDDM).  The use of 100 kg of N ha-1 contributed to an increase in CP and a decrease in NDF. The AMG 521 strain contributed to a smaller diameter of the root. Strains CCTB03 and AMG 521 demonstrated a smaller area, length and root density when associated with the dose of 50kg of N ha-1. In general, the PGPB were not efficient in promoting productive increments in ruzigrass.

scholarly journals Biomass Yield, Nitrogen Content and Uptake, And Nutritive Value of Alfalfa Co-Inoculated with Plant-Growth Promoting Bacteria

2020 ◽  
Vol 8 (5) ◽  
pp. 400-420
Author(s):  
Cecilio Viega Soares Filho ◽  
Leonardo Aurélio Silva ◽  
Jaqueline Silva Boregio ◽  
Mariangela Hungria ◽  
Adônis Moreira ◽  
...  
Keyword(s):  
Plant Growth ◽  
Sinorhizobium Meliloti ◽  
Nutritive Value ◽  
Dry Weight ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
Plant Growth Promoting Bacteria ◽  
Total N ◽  
Plant Growth Promoting ◽  
Growth Promoting

Alfalfa (Medicago sativa L.) has high forage yield potential, protein quality, palatability, and digestibility, and low seasonality. The aim of this study was to evaluate the effects of strains of Sinorhizobium meliloti and Azospirillum brasilense on the nutritive content, bromatological composition (crude protein [CP], neutral detergent fiber [NDF], acid detergent fiber [ADF], and in vitro digestibility of dry weight [IVDDW]), and shoot dry weight (SDW), relative chlorophyll index (RCI), number of tillers (NT), plant height (PH), and root dry weight (RDW) and volume (RV), of alfalfa grown in a Typic Ultisol. The experiment consisted of eight combinations of plant-growth promoting bacteria (PGPB). The treatments were as follows: T1:non-inoculated control without N-fertilizer (NI); T2: NI + N-fertilizer, and inoculated with T3: Sinorhizobium (=Ensifer) meliloti SEMIA 116 + N-fertilizer; T4: S. meliloti SEMIA 116 + A. brasilense Ab-V5 + Ab-V6 + N-fertilizer; T5: S. meliloti SEMIA 134 + N-fertilizer; T6: S. meliloti SEMIA 134 + co-inoculation + N-fertilizer; T7: S. meliloti SEMIA 135 + N-fertilizer; and T8: S. meliloti SEMIA 135 + co-inoculation + N-fertilizer. S. meliloti strains are used in commercial inoculants for the alfalfa, and A. brasilense for several non-legumes and legumes in Brazil. The experiment was performed for three successive cuts under greenhouse conditions. Application of N-fertilization increases the production cost, making alfalfa cultivation unviable. Inoculation with three strains of Sinorhizobium meliloti highly promoted alfalfa growth, considering several parameters, including PH, RCI, NT, SDW and RDW, nutritive value, and with an emphasis on RV, and total N content and total N accumulated in shoots and roots. No further increases were observed with the co-inoculation with the PGPB A. brasilense. Studies in field and greenhouse conditions are necessary to verify the benefits of the use of PGPB in the cultivation of alfalfa.

scholarly journals Nitrogen in Shoots, Number of Tillers, Biomass Yield and Nutritive Value of Zuri Guinea Grass Inoculated with Plant-Growth Promoting Bacteria

2020 ◽  
Vol 8 (5) ◽  
pp. 437-463
Author(s):  
Cecilio Viega Soares Filho ◽  
Caroline Lopes Monteiro de Carvalho ◽  
Mariangela Hungria ◽  
Marco Antônio Nogueira ◽  
Adônis Moreira ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Biomass Yield ◽  
N Uptake ◽  
N Fertilizer ◽  
Plant Growth Promoting Bacteria ◽  
Rhizobium Tropici ◽  
Total N ◽  
Plant Growth Promoting ◽  
Guinea Grass ◽  
Growth Promoting

The objective of this study was to evaluate the effects of plant growth-promoting bacteria (PGPB) strains of Azospirillum brasilense, Pseudomonas fluorescens and Rhizobium tropici on biomass yield, number of tillers, nitrogen accumulation and nutritive value of shoots of Megathyrsus (syn. Panicum) maximus cultivar BRS Zuri (Zuri Guinea grass). For that, one experiment was performed for 14 months to evaluate inoculation and re-inoculation with Azospirillum brasilense strains Ab-V5 and Ab-V6, Pseudomonas fluorescens strain CCTB 03 and of co-inoculation with Rhizobium tropici strain CIAT 899 + A. brasilense strain Ab-V6 combined with N-fertilizer (100 kg of N ha-1). Shoot dry weight yield (SDWY), number of tillers (NT), total N concentration (TNC), total N uptake (TNU) and nutritive value of Zuri Guinea grass was evaluated for eight cuts, and inoculation increased all parameters. In the NT, the treatments inoculated with PGPB were superior to the positive non-inoculated control receiving N-fertilizer, by up to 36%. For the accumulated of SDWY the treatment re-inoculated with P. fluorescens CCTB 03 after each cut was statistically superior tin 7% the positive control. The PGPB when combined N-fertilizer also increased SDWY, NT, the relative chlorophyll index, TNC, total N uptake, neutral detergent fiber, acid detergent fiber, crude protein and in vitro digestibility dry matter of Zuri Guinea grass. The results indicate that PGPB can represent a sustainable alternative for reducing the use of N-fertilizers. The lower effects of re-inoculation with PGPB on the nutrition or yield of Zuri Guinea grass, demonstrating that the determination of the method of application and periodicity of inoculation still require investigation.

scholarly journals 68 Effects of Plant Growth-Promoting Rhizobacteria on Fall-Stockpiled Bermudagrass

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 31-32
Author(s):  
Madison Cole ◽  
Megan E Griffin ◽  
Sandra L Dillard ◽  
Mary K Mullenix ◽  
Russ B Muntifering ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutritive Value ◽  
Plant Growth Promoting Rhizobacteria ◽  
N Fertilizer ◽  
Alternative Form ◽  
Forage Production ◽  
Synthetic Fertilizer ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Forage production practices have been greatly affected by the increasing cost of N fertilization. Therefore, supplemental and alternative N sources are needed to ensure the economic viability of these systems. A 2-yr, small plot study was designed to evaluate plant growth-promoting rhizobacteria (PGPR) as an alternative form of N fertilizer for fall-stockpiled bermudagrass (Cynodon dactylon). Eighteen 1-m2 ‘Coastal’ bermudagrass plots were treated with a synthetic N fertilizer, DH44 (PGPR strain), DH44+fertilizer, Blend 20 (PGPR blend), Blend 20+fertilizer, and a control, then stockpiled through the fall. Two PGPR applications were applied in late-August and again 30 d later. Fertilizer and PGPR+fertilizer plots received (NH4)2SO4, at a rate of 56 kg N/ha. One-third of each plot was clipped to 2.5 cm in November, December, and January, respectively. Forage DM yield, CP, NDF, ADF, and ADL were determined via wet chemistry at the Auburn University Ruminant Nutrition Laboratory (Auburn, AL). Data were analyzed using PROC MIXED (SAS 9.4, SAS Inst., Cary, NC) as a completely randomized design. Yield was greatest (P ≤ 0.0318) for Blend 20+fertilizer, but it was not different (P = 0.2552) from that of the synthetic fertilizer (1,914 kg ha-1, 1,768 kg ha-1, respectively). Concentration of CP was least (P ≤ 0.0437) for DH44 and Blend 20 treatments (90 g kg-1 and 92 g kg-1, respectively). Concentrations of NDF for the control were different (P ≤ 0.0045) for all treatments except synthetic fertilizer (P = 0.1092). Concentrations of ADF were not different (P ≥ 0.1613) excluding the control (P ≤ 0.0525; 342.8 g kg-1and 358.0 g kg-1, respectively). In vitro true digestibility (IVTD) was not different (P = 0.0947) among all treatments (463.1 g kg-1). All yield and nutritive value parameters were greater (P ≤ 0.0246) in Year 2. These results indicate that PGPR is a viable option for biofertilization of fall-stockpiled bermudagrass; however, further investigation into the effects of PGPR inoculants at a field scale are needed.

scholarly journals Maize response to inoculation with strains of plant growth-promoting bactéria

2016 ◽  
Vol 20 (7) ◽  
pp. 606-611 ◽  
Author(s):  
Janaína Dartora ◽  
Vandeir F. Guimarães ◽  
Cid R. J. Menezes ◽  
Mariângela B. Freiberger ◽  
Gustavo Castoldi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Block Design ◽  
N Fertilization ◽  
Plant Growth Promoting Bacteria ◽  
Maize Seeds ◽  
Randomized Block Design ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Maize Response ◽  
Rhizobium Sp

ABSTRACT The aim of this study was to evaluate the response of maize to inoculation with strains of plant growth-promoting bacteria (PGPB) in two cultivation years. The experiment was set in a randomized block design with four replicates in two cultivation years (2012/13 and 2013/14). The treatments consisted of PGPB inoculation: control (without N and without inoculation); 30 kg of N ha-1 at sowing (N1); 160 kg of N ha-1 (N1 + 130 kg of N ha-1 as top-dressing); N1 + A. brasilense, Ab-V5; N1 + A. brasilense, HM053; N1 + Azospirillum sp. L26; N1 + Azospirillum sp. L27; N1 + Enhydrobacter sp. 4331; N1 + Rhizobium sp. 8121. Basal stem diameter, plant height, leaf area, shoot dry matter and yield were evaluated. The strain of Rhizobium sp. 8121and the isolate Azospirillum sp. L26 associated with 30 kg of N ha-1 at sowing promoted yields equivalent to that of the N fertilization of 160 kg ha-1, demonstrating the potential to be used in the inoculation of maize seeds.

scholarly journals Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance

Bragantia ◽  
2016 ◽  
Vol 75 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Leandro Teodoski Spolaor ◽  
Leandro Simões Azeredo Gonçalves ◽  
Odair José Andrade Pais dos Santos ◽  
André Luiz Martinez de Oliveira ◽  
Carlos Alberto Scapim ◽  
...  
Keyword(s):  
Plant Growth ◽  
Grain Yield ◽  
Nitrogen Fertilization ◽  
Block Design ◽  
N Fertilization ◽  
Plant Growth Promoting Bacteria ◽  
Promising Alternative ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
N Rates

ABSTRACT The use of plant growth-promoting bacteria is a promising alternative with low environmental impact to increase the efficiency of use of chemical fertilizers, ensuring high yield with better cost-effective ratio. In maize crops, several studies have demonstrated an increased yield when Azospirillum-based inoculants are used. In the case of popcorn, there are no available studies related to use of inoculation and its response on yield parameters. Thus, the aim of this study was to evaluate the field performance of popcorn when inoculated with the commercial product Masterfix L (A. brasilense Ab-V5 and A. brasilense Ab-V6) and the non-commercial inoculant UEL (A. brasilense Ab-V5 + Rhizobium sp. 53GRM1) associated with nitrogen fertilization. The trials were conducted in Londrina and Maringá, Paraná State, Brazil, in a randomized block design with four replications, in a split plot design with the inoculation treatments located in the plots (uninoculated, Masterfix L, and UEL) and the different N rates located in the subplots where ammonium sulphate was applied in the topdressing at the V6 stage (0, 50, 100, and 150 kg∙ha–1). The variance analysis showed significant effects (p < 0.05) of inoculation (Londrina environment) and N rates (both environments) only for grain yield. There was no inoculation effect in the grain yield when inoculants were applied together with N-fertilization at topdressing. In the absence of N-fertilization at topdressing, the inoculants Masterfix L. and UEL promoted higher grain yield as compared to the uninoculated plants, with resulting increases of 13.21 and 26.61% in yield, respectively.

scholarly journals Inoculation with plant growth-promoting bacteria and reduction of nitrogen fertilizer in herbage accumulation and nutritional value of Mavuno grass

2021 ◽  
Vol 9 (3) ◽  
pp. 16-34
Author(s):  
Amário Nuno Meireles Duarte ◽  
Cecílio Viega Soares Filho ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Caroline Lopes Monteiro de Carvalho ◽  
Mariangela Hungria ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutritional Value ◽  
Block Design ◽  
Control Treatment ◽  
Plant Growth Promoting Bacteria ◽  
Rhizobium Tropici ◽  
Mineral N ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Insoluble Fiber

Strategies that improve the use of nitrogen (N), by tropical forage grasses, can bring environmental and social gains. Thus, this study aimed to evaluate the forage productivity and the nutritional value (NV) of the hybrid Urochloa spp. cv. ´Mavuno`, under inoculation with plant growth-promoting bacteria (PGPB) and doses of N. The experiment was carried out in the field, for a period of 14 months, in a randomized block design, with nine treatments and four replications in plots of 9 m². Seed inoculations were tested with: Azospirillum brasilense Ab-V5 and Ab-V6 strains; Pseudomonas fluorescens CCTB 03 and co-inoculation with Rhizobium tropici CIAT 899 and A. brasilense Ab-V6, combined with 50 and 100 kg ha-1 of added mineral N; fertilization with N-mineral with these doses, without inoculation and the control treatment, without N and without inoculation. The bacteria were inoculated to the grass seeds. The forage was evaluated by determining the shoot dry weight yield (SDWY) and the levels of crude protein (CP), neutral detergent insoluble fiber (NDF), acid detergent insoluble fiber (ADF) and in vitro dry matter digestibility (IVDMD). The results were subjected to analysis of variance by the F test (p ≤ 0.05) and compared to each other by the Scott-Knott test at 5% probability. Inoculation with PGPB resulted in greater SDWY when associated with a dose of 50 kg ha-1 of N. In plants inoculated with A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with a dose of 50 kg ha-1 of N-mineral, SDWY was similar to that obtained with double the dose of N in the absence of inoculation. The inoculation with PGPB resulted in an increase in the NV of Mavuno grass, with emphasis on A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with the dose of 100 kg ha-1 of N-mineral that improved the nutritional value in all evaluated items. The inoculation with A. brasilense Ab-V5 + Ab-V6 was the most promising, followed by P. fluorescens CCTB 03, mainly in association with 50 kg ha-1 of N.

scholarly journals Nutrients Uptake in Shoots and Biomass Yields and Roots and Nutritive Value of Zuri Guinea Grass Inoculated with Plant Growth-promoting Bacteria

2019 ◽  
Vol 50 (22) ◽  
pp. 2927-2940
Author(s):  
Geovana C. R. Sá ◽  
Mariangela Hungria ◽  
Caroline Lopes Monteiro Carvalho ◽  
Adônis Moreira ◽  
Marco Nogueira ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutritive Value ◽  
Plant Growth Promoting Bacteria ◽  
Nutrients Uptake ◽  
Plant Growth Promoting ◽  
Biomass Yields ◽  
Guinea Grass ◽  
Growth Promoting

scholarly journals Bacterial Consortium for Improved Maize (Zea mays L.) Production

2019 ◽  
Vol 7 (11) ◽  
pp. 519 ◽  
Author(s):  
Oluwaseyi Samuel Olanrewaju ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Bacterial Consortium ◽  
Microbial Consortia ◽  
Plant Growth Promoting Bacteria ◽  
Promoting Effect ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

The ever-increasing human population is a major concern for food security. Maize is the third largest most important food crop. The major problems of cultivation arise from urbanization and land pollution. This reduces the amount of land available for agriculture. The use of chemicals in agriculture is not environmentally friendly. Thus, plant growth-promoting bacteria (PGPB) have been proposed as alternatives. This study aims to test the growth-promoting effect of maize inoculated with six indigenous PGPB isolates. These isolates were assayed for various biochemical and plant growth-promoting activities. They were also assayed for biocontrol activities. Based on the results, six isolates viz A1, A18, A29, NWU4, NWU14, and NWU198 were used to inoculate maize seeds. The inoculated seeds were tried out on the field. A randomized block design was used. PGPB used were in single, consortia of two, and three organisms. The length of the leaves, roots, and stem, plant height, numbers of leaves, and weight of 100 seeds were taken at the fourth and eighth weeks after planting. Microbial consortia increased growth parameters compared to single inoculant treatments. Thus, they can be of advantage in the eradication of low yield. They can also serve as reliable alternatives to chemical fertilizers.

scholarly journals Yield, yield components and nutrients uptake in Zuri Guinea grass inoculated with plant growth-promoting bacteria

2020 ◽  
Vol 8 (4) ◽  
pp. 103-124
Author(s):  
Gilmar Cotrin de Lima ◽  
Mariangela Hungria ◽  
Marco Antonio Nogueira ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Adônis Moreira ◽  
...  
Keyword(s):  
Plant Growth ◽  
N Fertilizer ◽  
Plant Growth Promoting Bacteria ◽  
Rhizobium Tropici ◽  
Nutrients Uptake ◽  
Plant Growth Promoting ◽  
Guinea Grass ◽  
Growth Promoting ◽  
Chlorophyll Index

The objective of this study was to evaluate the effects of strains of Azospirillum brasilense, Pseudomonas fluorescens and Rhizobium tropici on biomass yield and nutrients uptake of shoots and roots of Megathyrsus (syn. Panicum) maximus cultivar BRS Zuri (Zuri Guinea grass) inoculated with plant growth-promoting bacteria (PGPB). Treatments consisted of inoculation and re-inoculation with A. brasilense strains Ab-V5 and Ab-V6, P. fluorescens strain CCTB 03 and of co-inoculation with R. tropici strain CIAT 899 + A. brasilense Ab-V6, with or without N-fertilizer (100 mg dm-3). Evaluations were performed on three cuts for the determination of root and shoot dry weight yield, morphological compositions, tiller mass, number of tillers, and nutrient uptake. Inoculation with bacteria in association with N-fertilizer increased N, NH4+, Ca, Fe, Mn and Zn accumulation in shoots and P and K uptake in roots. P. fluorescens and co-inoculation with R. tropici CIAT 899 + A. brasilense Ab-V6 increased the relative chlorophyll index in relation to the non-inoculated control. As expected, PGPB were not able to fully replace N-fertilization. However, when combined with N-fertilizer, the PGPB increased yield, the relative chlorophyll index, and the uptake of N, NH4+, Ca, Zn, Mn and Fe of Zuri Guinea grass. The results indicate that PGPB can represent a sustainable alternative for reducing the use of N-fertilizers. There were no effects of re-inoculation with PGPB on the nutrition or yield of Zuri Guinea grass, demonstrating that the determination of the method of application and periodicity of inoculation still require investigation.

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