scholarly journals Investigation of BDNF gene expression level by analysis of dysregulation of miRNA-15a: An in-silico approach

2021 ◽  
Vol 9 (8) ◽  
pp. 2293-2299
Author(s):  
Aditi Pareek
Keyword(s):  
Vascular Disease ◽  
Vision Loss ◽  
Amacrine Cells ◽  
Quantitative Detection ◽  
Diabetic Patients ◽  
Retinal Ganglion ◽  
Middle Income ◽  
Type 2 Diabetic ◽  
Type 2 Diabetic Retinopathy

Abstract: Type 2 Diabetic retinopath (T2DR) remains the leading cause of vision loss and preventable blindness in adults aged 20–74 years, particularly in middle-income and high-income countries. The pathogenesis of T2DR is a predominant cause of visual impairment and a range of hyperglycemia linked pathways have been implicated in the initiation and progression of this condition. Apparently in the past T2DR was solely considered a vascular disease as opposed to the present time where it is recognised as a neuro-vascular disease. Some pro-survival neurotrophins such as brain derived neurotrophic factor (BDNF) are considered to guard retinal ganglion and amacrine cells from degenerative. Significant reduction in the levels of BDNF have been witnessed in diabetic patients as well as animal models. miRNAs are a group of 21-23 nucleotide long, highly conserved sequences of endogenous RNAs that do not encode for any protein. Researches carried out over the last decade gives plenty of proof about the miR-15a importance in T2DR. Henceforth, miR15a could be used for the experimental purposes. miRNAs can be considered as an efficient biomarker as they maintain their stability and utility over rigorous processing phases and the presence of quantitative detection boosts their therapeutical significance. Keywords: Type 2 Diabetic Retinopathy, Neurodegeneration, BDNF, Prognosis, Biomarker, miR-15a, Bioinformatics

scholarly journals Malondialdehyde, glutathione, glutathione peroxidase and homocysteine levels in type 2 diabetic patients with and without microalbuminuria

2005 ◽  
Vol 42 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Gülsen Ozdemır ◽  
Meltem Ozden ◽  
Hale Maral ◽  
Sevinc Kuskay ◽  
Pinar Cetınalp ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Vascular Disease ◽  
Healthy Subjects ◽  
Serum Levels ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Significant Difference ◽  
Total Homocysteine ◽  
Type 2 Diabetic

Background: High levels of homocysteine and oxidative stress are known to be associated with premature vascular disease in type 2 diabetes mellitus (DM). The aim of this study was to estimate homocysteine levels and oxidant-antioxidant status and to determine the relationship between them in type 2 diabetic patients with and without microalbuminuria. Methods: Fasting blood samples were obtained from 48 diabetic patients (17 with and 31 without microalbuminuria) and 20 healthy subjects. Serum total homocysteine (tHcy), plasma malondialdehyde (MDA) erythrocyte glutathione (GSH) and glutathione peroxidase (GPx) activity were measured in these patients and the results were compared with those of controls who were chosen among healthy subjects. Results: MDA levels were found to be significantly lower and GSH levels and GPx activities were found to be significantly higher in control subjects when compared with patients with and without microalbuminuria (MDA: P<0.0001, P<0.0001; GSH: P<0.0001, P<0.0001; GPx: P<0.0001, P<0.001, respectively). MDA levels were found to be significantly higher in patients with microalbuminuria compared with patients without microalbuminuria ( P<0.0001), while similarly GSH levels were found to be significantly lower in patients with microalbuminuria ( P<0.0001). Although there were no significant differences with respect to tHcy levels and GPx activities between the microalbuminuric and normoalbuminuric patients ( P>0.05), there was a significant difference with respect to tHcy levels between healthy controls and patients with microalbuminuria ( P<0.05). The serum levels of tHcy correlated best with plasma MDA and erythrocyte GSH concentrations in all diabetic patients ( r=0.549, P<0.0001; r=0.385, P<0.01). Conclusion: Decreased antioxidant levels, increased lipid peroxidation and increased tHcy levels were observed in patients with microalbuminuria. These changes may contribute to vascular disease, which is particularly prevalent in type 2 DM patients with microalbuminuria.

Middle Cerebral Artery Stenosis Increased the Risk of Vascular Disease Mortality among Type 2 Diabetic Patients

2008 ◽  
Vol 25 (3) ◽  
pp. 261-267 ◽  
Author(s):  
G. Neil Thomas ◽  
Xiang-Yan Chen ◽  
Jian Wen Lin ◽  
Brian Tomlinson ◽  
Wynnie W.M. Lam ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Vascular Disease ◽  
Cerebral Artery ◽  
Artery Stenosis ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Disease Mortality ◽  
Middle Cerebral Artery Stenosis ◽  
Type 2 Diabetic

scholarly journals Leukocyte Lipid Peroxidation, Superoxide Dismutase and Catalase Activities of Type 2 Diabetic Patients with Retinopathy

2005 ◽  
Vol 48 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Naciye Kurtul ◽  
Ebubekir Bakan ◽  
Hülya Aksoy ◽  
Orhan Baykal
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Diabetic Retinopathy ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Significant Difference ◽  
Type 2 Diabetic ◽  
Type 2 Diabetic Retinopathy

Increased oxidative stress might play an important role in the initiation and progression of diabetic complications. The present study has been undertaken to investigate whether there is any relationship between retinopathy degree and leukocyte superoxide dismutase (SOD) and catalase (CAT) activities and lipid peroxidation (LPO) in diabetic individuals with type 2 diabetic retinopathy. Patients were groupped with respect to the degree of retinopathy. Leukocyte malondialdehyde (MDA) levels, and SOD and CAT activities were measured in patients with type 2 diabetes mellitus (n=41) and nondiabetic healthy controls (n=23). Leukocyte LPO of the type 2 diabetic patients with retinopathy was significantly increased (p< 0.001), whereas SOD and CAT activities were decreased (p<0.001 and p<0.001, respectively) compared to those of controls. MDA concentrations rose while SOD and CAT activities fell with increasing severity of diabetic retinopathy, altough there was no significant difference in comprasion of the parameters mentioned above between the diabetic patients with and without retinopathy. Our results show that leukocytes in patients with type 2 diabetic retinopathy are affected by oxidative stress which might be contribute to pathogenesis of diabetic retinopathy. Prospective studies are needed to evaulate the relationship between the leukocyte antioxidants status and DR.

scholarly journals Natural History of Diabetic Retinopathy Through Retrospective Analysis in Type 2 Diabetic Patients—An Exploratory Study

2021 ◽  
Vol 9 ◽  
Author(s):  
Mehak Gupta ◽  
Amarjeet Singh ◽  
Mona Duggal ◽  
Ramandeep Singh ◽  
Sanjay Bhadada ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Natural History ◽  
Medical Records ◽  
Vision Loss ◽  
Adult Population ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
History Of ◽  
Type 2 Diabetic

Background and Aims: Diabetic retinopathy is the most common diabetes-associated microvascular complication and is among the leading causes of vision loss or blindness in the adult population. The present study is a retrospective study that reported the natural history of diabetic retinopathy.Methods: Retrospective medical records of 170 patients aged &gt; 20 years with a confirmed complication of diabetic retinopathy were recruited into the present study. A questionnaire was also sent to each subject for gathering their experiences, and verification was done by the attending medical physicians. The questionnaire was answered by all recruited patients.Results: The results showed that 23 (13.5%) subjects have a family history of diabetic retinopathy with 10 (5.9%) having mild NPDR, 63 (37.1%) with moderate NPDR, 60 (25.3%) have severe NPDR while 37 (21.8%) have PDR complications. The presence of co-morbidities was found in 139 (81.8%) subjects. Patients with PDR reported a significantly longer duration of diabetes mellitus with worse glycemic control.Conclusions: The study revealed and concluded that adherence to the prescribed management regimen is important, for which patient education was the key which was lacking.

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