scholarly journals Allelic variation at the Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of Pakistani spring wheat cultivars

Author(s):  
Muhammad Iqbal ◽  
Armghan Shahzad ◽  
Iftikhar Ahmed
2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Enid Perez-Lara ◽  
Kassa Semagn ◽  
Hua Chen ◽  
Van Anh Tran ◽  
Izabela Ciechanowska ◽  
...  

2012 ◽  
Vol 37 (11) ◽  
pp. 1917-1925 ◽  
Author(s):  
Fang-Ping YANG ◽  
Li-Ming HAN ◽  
Jun YAN ◽  
Xian-Chun XIA ◽  
Yong ZHANG ◽  
...  

2021 ◽  
Vol 22 (14) ◽  
pp. 7709
Author(s):  
Kyoungwon Cho ◽  
You-Ran Jang ◽  
Sun-Hyung Lim ◽  
Susan B. Altenbach ◽  
Yong Q. Gu ◽  
...  

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography–tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the ‘Aroona’ cultivar and 12 ‘Aroona’ near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for ‘Aroona’ and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in ‘Aroona’ and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement.


2016 ◽  
Vol 64 (22) ◽  
pp. 4545-4555 ◽  
Author(s):  
Thomas Etzerodt ◽  
Rene Gislum ◽  
Bente B. Laursen ◽  
Kirsten Heinrichson ◽  
Per L. Gregersen ◽  
...  

Crop Science ◽  
1996 ◽  
Vol 36 (4) ◽  
pp. 982-986 ◽  
Author(s):  
M. A. Moustafa ◽  
L. Boersma ◽  
W. E. Kronstad

2015 ◽  
Vol 48 (2) ◽  
pp. 51-60 ◽  
Author(s):  
Y. Shiri ◽  
M. Solouki ◽  
M. Forootan

AbstractIn order to study the allelic variation of Glu-1 gene (High molecular weight glutenin), 100 Iranian wheat cultivars including imported and domestic genotypes were analyzed using ALP-PCR technique. Four specific primer pairs were used based on the genetic loci of Glu-A1, Glu-B1, and Glu-D1 to perform the chain polymerase reactions. PCR reaction products were resolved on 2% agarose gel. Since allele “a” had the largest relative frequency (0.707), two alleles (a 344bp; b 362bp) were identified by P1-P2 primer for Glu-A1 locus. Three alleles (a 800bp; b 500bp; c 300bp) were detected for Glu-B1 locus by P5-P6 primer, and allele “b” was assumed as the highest relative frequency (0.618). Two primer pairs were applied for Glu-D1 locus. Ultimately, four alleles were identified, where allele “c” had the highest relative frequency (0.525). The observed genetic variation value for Glu-D1 locus (H=0.648) exhibits the maximal polymorphism. Using cluster analysis, the relationship between the observed polymorphism and geographical variation was investigated. The results indicated that there exists a remarkable variation in Glu- A1 locus between the Iranian wheat cultivars.


2020 ◽  
Vol 50 (2) ◽  
pp. 39-46
Author(s):  
A. A. Razina ◽  
F. S. Sultanov ◽  
О. G. Dyatlova

The results of studying resistance of mid-ripening spring wheat cultivars to root rot in the forest-steppe zone of Irkutsk region are presented. The study was conducted in a two-factor field experiment. Factor A – mid-ripening spring wheat cultivars: Tulunskaya 11 (control), Zoryana, Маrsianka, Stolypinka (new cultivars). Factor B – seeding dates: May 10, 20, 30, preceded by fallow. The experimental plot area was 70.0 m2. The experiment was repeated three times. Plot arrangement was randomized. Root rot prevalence was determined during the tillering phase of the crop. In 2018, the sowing dates did not affect the disease due to the long spring-summer drought (May-June) and a higher average daily temperature compared to long-term average values. In arid and cold conditions of May 2019, with the late sowing period (May 30), root rot prevalence was significantly lower than when sowing on May 10 and 20. In 2019, a higher level of the disease was registered than in 2018 by 14.3%. Significant differences in root rot prevalence in both years of research were noted between the control cultivar Tulunskaya 11 and the new cultivars of spring wheat Zoryana and Stolypinka. In the tillering phase of the latter two, disease indicators were lower by 5.6% and 10.5% in 2018, and by 8.8% and 7.9% in 2019, respectively. Маrsianka cultivar was at the control level for this indicator. The best cultivar under study was Stolypinka, which was not only less affected by root rot, but also gave a statistically significant yield increase of 0.16 t/ ha in 2018 and 0.22 t/ha in 2019.


Sign in / Sign up

Export Citation Format

Share Document