Distribution of Allelic Variation for Genes of Vernalization and Photoperiod among Wheat Cultivars from 23 Countries

2012 ◽  
Vol 37 (11) ◽  
pp. 1917-1925 ◽  
Author(s):  
Fang-Ping YANG ◽  
Li-Ming HAN ◽  
Jun YAN ◽  
Xian-Chun XIA ◽  
Yong ZHANG ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Wheat Cultivars

scholarly journals Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars

2021 ◽  
Vol 22 (14) ◽  
pp. 7709
Author(s):  
Kyoungwon Cho ◽  
You-Ran Jang ◽  
Sun-Hyung Lim ◽  
Susan B. Altenbach ◽  
Yong Q. Gu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Allelic Variation ◽  
Reversed Phase ◽  
Glutenin Subunit ◽  
Low Molecular Weight ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Reliable Determination ◽  
Isogenic Lines

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography–tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the ‘Aroona’ cultivar and 12 ‘Aroona’ near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for ‘Aroona’ and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in ‘Aroona’ and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement.

scholarly journals Analysis of Allelic Variation in Hmw-Glu-1 Gene Blocks in Iranian Wheat Cultivars using Alp Molecular Marker

2015 ◽  
Vol 48 (2) ◽  
pp. 51-60 ◽  
Author(s):  
Y. Shiri ◽  
M. Solouki ◽  
M. Forootan
Keyword(s):  
Molecular Weight ◽  
Cluster Analysis ◽  
Relative Frequency ◽  
Geographical Variation ◽  
Allelic Variation ◽  
Agarose Gel ◽  
Wheat Cultivars ◽  
Reaction Products ◽  
Specific Primer ◽  
The Relationship

AbstractIn order to study the allelic variation of Glu-1 gene (High molecular weight glutenin), 100 Iranian wheat cultivars including imported and domestic genotypes were analyzed using ALP-PCR technique. Four specific primer pairs were used based on the genetic loci of Glu-A1, Glu-B1, and Glu-D1 to perform the chain polymerase reactions. PCR reaction products were resolved on 2% agarose gel. Since allele “a” had the largest relative frequency (0.707), two alleles (a 344bp; b 362bp) were identified by P1-P2 primer for Glu-A1 locus. Three alleles (a 800bp; b 500bp; c 300bp) were detected for Glu-B1 locus by P5-P6 primer, and allele “b” was assumed as the highest relative frequency (0.618). Two primer pairs were applied for Glu-D1 locus. Ultimately, four alleles were identified, where allele “c” had the highest relative frequency (0.525). The observed genetic variation value for Glu-D1 locus (H=0.648) exhibits the maximal polymorphism. Using cluster analysis, the relationship between the observed polymorphism and geographical variation was investigated. The results indicated that there exists a remarkable variation in Glu- A1 locus between the Iranian wheat cultivars.

scholarly journals The Variability of Puroindoline-Encoding Alleles and Their Influence on Grain Hardness in Modern Wheat Cultivars Cultivated in Poland, Breeding Lines and Polish Old Landraces (Triticum aestivum L.)

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1075
Author(s):  
Mateusz Przyborowski ◽  
Sebastian Gasparis ◽  
Maciej Kała ◽  
Wacław Orczyk ◽  
Anna Nadolska-Orczyk
Keyword(s):  
Triticum Aestivum ◽  
System Analysis ◽  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Nucleotide Polymorphisms ◽  
Wheat Cultivars ◽  
Grain Hardness ◽  
Wild Type ◽  
Breeding Lines ◽  
Modern Wheat

Wheat (Triticum aestivum L.) grain hardness is determined mainly by variations in puroindoline genes (Pina-D1 and Pinb-D1), which are located on the short arm of chromosome 5D. This trait has a direct effect on the technological properties of the flour and the final product quality. The objective of the study was to analyze the mutation frequency in both Pin genes and their influence on grain hardness in 118 modern bread wheat cultivars and breeding lines cultivated in Poland, and 80 landraces from Poland. The PCR products containing the Pin gene coding sequences were sequenced by the Sanger method. Based on detected the SNPs (single-nucleotide polymorphisms) we designed CAPS (cleaved amplified polymorphic sequence) markers for the fast screening of Pinb alleles in a large number of genotypes. All analyzed cultivars, breeding lines, and landraces possess the wild-type Pina-D1a allele. Allelic variation was observed within the Pinb gene. The most frequently occurring allele in modern wheat cultivars and breeding lines (over 50%) was Pinb-D1b. The contribution of the remaining alleles (Pinb-D1a, Pinb-D1c, and Pinb-D1d) was much less (approx. 15% each). In landraces, the most frequent allele was Pinb-D1a (over 70%), followed by Pinb-D1b (21% frequency). Pinb-D1c and Pinb-D1g were found in individual varieties. SKCS (single-kernel characterization system) analysis revealed that grain hardness was strictly connected with Pinb gene allelic variation in most tested cultivars. The mean grain hardness values were significantly greater in cultivars with mutant Pinb variants as compared to those with the wild-type Pinb-D1a allele. Based on grain hardness measured by SKCS, we classified the analyzed cultivars and lines into different classes according to a previously proposed classification system.

scholarly journals Allelic Variation at Loci Associated with Grain Properties in Korean Wheat Cultivars

2020 ◽  
Vol 52 (4) ◽  
pp. 362-373
Author(s):  
Kyeong-Min Kim ◽  
Seong-Woo Cho ◽  
Ri Choe ◽  
Na Kyung Ryu ◽  
Jung-Yoon Hwang ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Wheat Cultivars ◽  
Grain Properties

scholarly journals Allelic Variation at the Vernalization Genes Vrn-A1 , Vrn-B1 , Vrn-D1 , and Vrn-B3 in Chinese Wheat Cultivars and Their Association with Growth Habit

Crop Science ◽  
2008 ◽  
Vol 48 (2) ◽  
pp. 458-470 ◽  
Author(s):  
X. K. Zhang ◽  
Y. G. Xiao ◽  
Y. Zhang ◽  
X. C. Xia ◽  
J. Dubcovsky ◽  
...  
Keyword(s):  
Growth Habit ◽  
Allelic Variation ◽  
Wheat Cultivars ◽  
Vernalization Genes ◽  
Chinese Wheat

Allelic variation and effects of 16 candidate genes on disease resistance in western Canadian spring wheat cultivars

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Enid Perez-Lara ◽  
Kassa Semagn ◽  
Hua Chen ◽  
Van Anh Tran ◽  
Izabela Ciechanowska ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Candidate Genes ◽  
Spring Wheat ◽  
Allelic Variation ◽  
Wheat Cultivars

scholarly journals Allelic Variation at theRht8Locus in a 19th Century Wheat Collection

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Linnéa Asplund ◽  
Matti W. Leino ◽  
Jenny Hagenblad
Keyword(s):  
Plant Breeding ◽  
20Th Century ◽  
19Th Century ◽  
Allelic Variation ◽  
Allelic Diversity ◽  
Wheat Breeding ◽  
Minor Role ◽  
Functional Markers ◽  
Wheat Cultivars ◽  
A Minor

Wheat breeding during the 20th century has put large efforts into reducing straw length and increasing harvest index. In the 1920s an allele ofRht8with dwarfing effects, found in the Japanese cultivar “Akakomugi,” was bred into European cultivars and subsequently spread over the world.Rht8has not been cloned, but the microsatellite marker WMS261 has been shown to be closely linked to it and is commonly used for genotypingRht8. The “Akakomugi” allele is strongly associated withWMS261-192bp. Numerous screens of wheat cultivars with different geographical origin have been performed to study the spread and influence of theWMS261-192bpduring 20th century plant breeding. However, the allelic diversity of WMS261 in wheat cultivars before modern plant breeding and introduction of the Japanese dwarfing genes is largely unknown. Here, we report a study of WMS261 allelic diversity in a historical wheat collection from 1865 representing worldwide major wheats at the time. The majority carried the previously reported 164 bp or 174 bp allele, but with little geographical correlation. In a few lines, a rare 182 bp fragment was found. Although straw length was recognized as an important character already in the 19th century,Rht8probably played a minor role for height variation. The use of WMS261 and other functional markers for analyses of historical specimens and characterization of historic crop traits is discussed.

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