scholarly journals Evaluation of Antiasthamic Activity of Electrohomeopathy Formulation Pettorale on Experimental Animals

2021 ◽  
Vol 11 (6) ◽  
pp. 121-126
Author(s):  
Prasant Kumar Sabat
Keyword(s):  
Amino Acids ◽  
Guinea Pig ◽  
Histamine Release ◽  
Antibody Production ◽  
Eosinophil Count ◽  
Leukocyte Count ◽  
Experimental Models ◽  
Phytochemical Screening ◽  
Experimental Animals ◽  
Egg Albumin

Objective: The current study was planned to evaluate the antiasthamatic effect of Electrohomeopathic medicine Pettorale in various experimental models. Methods: The antiasthmatic activity of Electrohomeopathic medicine Pettorale was studied on different experimental animals like histamine induced bronchospasm in guinea pig, haloperidol induced catalepsy in rats, egg albumin induced paw anaphylaxis in rats and milk induced leukocytosis in mice. Conclusion: Preliminary phytochemical screening has revealed the presence of alkaloids, glycosides, carbohydrates, amino acids, proteins, steroids and terpenoids. Petorella exhibited best antihistaminic activity at the dose of 400 mg/kg.  It inhibited haloperidol-induced catalepsy, increased leukocyte count and increased eosinophil count due to milk allergen. Antiasthmatic activity of Pettorale may be possible due to the membrane stabilising potential, suppression of antibody production and inhibition of antigen induced histamine release. Keywords: Electrohomeopathy,   Pettorale, catalepsy, asthma, histamine

Antigen-induced histamine release by penetration of egg albumin antigen through excised guinea pig skin

1981 ◽  
Vol 271 (3) ◽  
pp. 357-360 ◽  
Author(s):  
S. Yamamoto ◽  
I. Kimura ◽  
T. Numata ◽  
T. Yamura
Keyword(s):  
Guinea Pig ◽  
Histamine Release ◽  
Egg Albumin ◽  
Pig Skin

Evaluation of Antiasthmatic Activity of Murraya Koenigii L. Leaves

2017 ◽  
Vol 3 (2) ◽  
pp. 285-293
Author(s):  
Jitendra Singh Rathore ◽  
Umesh Gilhotra
Keyword(s):  
Body Weight ◽  
Guinea Pig ◽  
Potential Role ◽  
Leukocyte Count ◽  
Ethanolic Extract ◽  
Total Leukocyte Count ◽  
Significant Protection ◽  
Murraya Koenigii ◽  
Experimental Animals ◽  
Dose Dependent

The present investigation was designed to evaluate the anti-asthmatic activity of ethanolic extract of Murraya Koenigii Leaves in experimental animals. Murraya Koenigii Leaves was evaluated for anti-histaminic activity using isolated Guinea pig tracheal chain preparation, histamine induced Bronchoconstriction in Guinea pig and milk induced leucocytosis in mice. Ethanolic extract of Murraya Koenigii Leaves oral dose 400mg/kg body weight significantly inhibited dose dependent contraction of Guinea pig tracheal chain produced by histamine and also showed significant protection by prolonging PreConvulsion Dyspnoea time (PCT) in guinea pigs. Murraya Koenigii Leaves was evaluated by milk induced leucocytosis in mice showed significant decreased of total leukocyte count. Thus, Murraya Koenigii Leaves showed anti-allergic activity against histamine hence possesses potential role in the treatment of asthma.

Epithelial modulation of allergen and drug effects in guinea pig airways

1992 ◽  
Vol 72 (5) ◽  
pp. 1953-1959 ◽  
Author(s):  
N. Grundstrom ◽  
E. G. Lindstrom ◽  
K. L. Axelsson ◽  
R. G. Andersson
Keyword(s):  
Guinea Pig ◽  
Histamine Release ◽  
Contractile Response ◽  
Muscle Tension ◽  
Drug Effects ◽  
Potassium Ions ◽  
Epithelial Damage ◽  
Egg Albumin

The effect of egg albumin (EA) challenge on tracheal tube preparations from sensitized guinea pigs was studied with regard to EA permeability, histamine release and penetrability, and the contractile response of the preparation. We used a plethysmographic method that allowed simultaneous measurement of changes in smooth muscle tension and collection of samples for determination of mediators. Our results clearly show that epithelial damage potentiates the contractile response to histamine, potassium ions, and acetylcholine. Epithelial damage did not alter the maximal contractile response in preparations challenged with high antigen concentrations (EA, 1 mg/ml), but histamine release measured in the perfusate increased substantially. The permeability of the preparations to EA was greater when the epithelium was damaged. No increase in the permeability in response to the EA challenge was observed. The present study has demonstrated that guinea pig airway epithelium constitutes a barrier for both antigen and drugs. We also present a method for recording contractile responses from intact whole tracheal preparations, in which the epithelium can still act as a barrier, as is the case in vivo.

scholarly journals Effects of epithelium on antigen-induced histamine release from guinea-pig isolated trachea

1990 ◽  
Vol 52 ◽  
pp. 306
Author(s):  
Katsuyoshi Sugizaki ◽  
Tomokazu Hosokawa ◽  
Yutaka Kasuya
Keyword(s):  
Guinea Pig ◽  
Histamine Release ◽  
Isolated Trachea

scholarly journals THE EFFECT OF ADMINISTRATION OF EPINEPHRINE ON THE LEUKOCYTE COUNTS OF NORMAL SUBJECTS

Blood ◽  
1950 ◽  
Vol 5 (8) ◽  
pp. 723-731 ◽  
Author(s):  
COLIN WHITE ◽  
TSUIN HWA LING ◽  
ARNOLD M. KLEIN
Keyword(s):  
Eosinophil Count ◽  
Adrenocorticotropic Hormone ◽  
Leukocyte Count ◽  
Normal Subjects ◽  
Total Count ◽  
Subcutaneous Injections ◽  
History Of ◽  
Leukocyte Counts ◽  
Adrenocortical Hormone ◽  
The One

Abstract 1. Thirty-seven normal subjects were given subcutaneous injections of epinephrine, ranging from 0.25 to 0.5 mg., and the effects on the leukocytes were noted. 2. The neutrophils rose steadily for the three and one-half hours during which blood counts were made. The small lymphocytes rose in the first half hour, then fell below normal and finally returned towards normal. The eosinophils rose at first and then fell below normal for the remainder of the period. 3. The three doses of epinephrine used produced effects which differed quantitatively but not qualitatively. The most readily identified effect of the smallest dose was the one-half hour rise in lymphocytes or the one-half hour rise in total count. A dose of 0.5 mg. is satisfactory for work of this kind. 4. Subjects with a history of allergy showed a greater tendency than the remainder to exhibit a one-half hour rise in the eosinophil count. 5. The changes in the leukocyte count produced by epinephrine are similar to, but not identical with, those produced by adrenocortical hormone or adrenocorticotropic hormone.

Ethanol inhibition of antigen-induced histamine release from guinea pig lung: Correlation with intracellular levels of cyclic nucleotides

Life Sciences ◽  
1979 ◽  
Vol 24 (25) ◽  
pp. 2321-2331 ◽  
Author(s):  
Michael J. Schmidt ◽  
Lewis L. Truex ◽  
Carol S. Hooker ◽  
Stephen M. Spaethe ◽  
Jerome H. Fleisch
Keyword(s):  
Guinea Pig ◽  
Histamine Release ◽  
Cyclic Nucleotides ◽  
Ethanol Inhibition

scholarly journals IN-VIVO ANTI-DIARRHOEAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL STEM BARK EXTRACT OF VITELLARIA PARADOXA GAERTN F. (SAPOTACEAE)

2020 ◽  
Vol 4 (3) ◽  
pp. 247-251
Author(s):  
Z. Abdullahi ◽  
A. A. Jimoh ◽  
B. E. Patrick ◽  
M. I. Yakubu ◽  
D. Mallam
Keyword(s):  
Castor Oil ◽  
Lethal Dose ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Experimental Models ◽  
Bark Extract ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Stem Bark Extract

Different parts of Vitellaria paradoxa plant have many applications in ethno-medicine. Some of the uses of this plant include treatment of diarrhoea and other GIT disorders. In this study the antidiarrhoeal activity of the ethanol extract of Vitellaria paradoxa was evaluated using three experimental models: Castor oil-induced diarrhoea; small intestinal motility and intestinal fluid accumulation (enteropooling) models in mice. Five groups of five mice were used for each model. Group one mice received 10 ml/kg of distilled water, while groups 2, 3, and 4 received 125, 250 and 500 mg/kg of the extract orally respectively. Group 5 mice received Loperamide 5 mg/kg orally. Oral median lethal dose (LD50) of the extract was determined using OECD (2008) Guideline 425. Phytochemical studies were conducted using standard procedures. The LD50 was estimated to be greater than 5000 mg/kg body weight and there were no signs of mortality or visible signs of toxicity in all the mice treated. Phytochemical screening revealed the presence of carbohydrates, alkaloids, flavonoids, saponins, tannins, triterpenes, steroids, cardiac glycosides and anthraquinones glycosides. Extract showed a dose-dependent anti-diarrhoeal activity by reducing stool frequency and consistency. The extract at the higher doses significantly (p < 0.05) inhibited GIT motility and castor oil-induced enteropooling, comparable to that of the reference control drug Loperamide. The study showed that ethanol stem bark extract of Vitellaria paradoxa possess anti-diarrhoeal activity and thus justifies its ethno-medicinal use in the treatment of diarrhoea.

scholarly journals Anti-Inflammatory, Analgesic and Antipyretic Activity of Catunaregam spinosa (Thumb.) Tirveng Extracts

2019 ◽  
Vol 9 (5) ◽  
pp. 89-94
Author(s):  
Hansa Saini ◽  
Jayesh Dwivedi ◽  
Himanshu Paliwal ◽  
Udichi Kataria ◽  
Priyanka Chauhan ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Pharmacological Properties ◽  
Phytochemical Screening ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Egg Albumin ◽  
Antipyretic Activity ◽  
Ethanolic Extracts ◽  
Anti Inflammatory ◽  
Response Method

Catunaregam spinosa leaves have been ethnopharmacologically accounted for acquiring various pharmacological properties. The present study was undertaken to evaluate anti-inflammatory, analgesic and antipyretic potential of leaves of C. spinosa.The ethanolic extract was selected for this purpose based on phytochemical screening. Inflammation was inhibited at the dose of 200 mg/kg with percent inhibition of inflammation 32.06, 37.28 and 43.16 %, respectively, at 1, 3 and 5 h, while in egg albumin model % inhibition was found to be 47.81%. There was no significant analgesic activity seen in acetic acid induced writhing response method while significant effects were observed in the doses of 25 and 100 mg/kg on hot plate test. No antipyretic activity was shown by ethanolic extracts(25, 100 and 200 mg/kg) against Brewer’s yeast induced pyrexia in rats. Keywords: Catunaregam spinosa, Anti-inflammatory activity, Phytochemical screening, Ethanolic extract.

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