scholarly journals Growth Response of Two Pepper (Piper nigrum L.) Stem Cuttings on Application of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid)

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Rizky Wulandari ◽  
Yaya Hasanah ◽  
Meiriani Meiriani
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Growth Response ◽  
Block Design ◽  
Primary Branch ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Secondary Branch ◽  
Indole Butyric Acid ◽  
Significant Difference

Using fruit branch for pepper shrub propagation is one of alternatives for an efficient pepper multiplication which usually uses underlayer cuttings. This research is aimed at finding the growth response of two pepper (Piper nigrum L.) cuttings to the administration of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid). This research was conducted at the greenhouse of the Faculty of Agriculture, University of Sumatera Utara, Medan (± 32 m above sea level), from April to August 2017 using a factorial randomized block design with 2 factors, pepper branch cuttings (primary branch cuttings and secondary branch cuttings) and the administration of IBA and NAA (0+0 ppm, 2500+0 ppm, 0+2500 ppm, 1500+1000 ppm, and 1000+1500 ppm). The results show that the emerging shoot rate in the secondary branch cuttings was significantly faster than in the primary branch cuttings, but the volume of root in the primary branch cuttings is significantly larger than in the secondary branch cuttings. There was no significant difference in the administration of IBA and NAA on all observed variables. The highest interaction of shoot length was found in the  primary branch cuttings with the administration of  IBA 1500 ppm + NAA 1000 ppm and the highest percentage of root and root volume was found in the primary branch cuttings with the administration of IBA 2500 ppm + NAA 0 ppm.

scholarly journals Rooted cuttings by IBA (Indole butyric acid) and NAA (Naphthalene acetic acid) in black pepper (piper nigrum) - A case study in Vietnam

2020 ◽  
Vol 8 (3) ◽  
pp. 1880-1885
Author(s):  
Tran Quyen Nguyen ◽  
Ba Huy Nguyen ◽  
Dieu Hien Tran Thi ◽  
Oanh Duong Thi ◽  
Quang Ngoc Nguyen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Rooted Cuttings

scholarly journals PENGARUH PEMBERIAN IBA (Indole Butyric Acid) DAN KONSENTRASI NAA (Naphthalene Acetic Acid) TERHADAP KEBERHASILAN PENYETEKAN SIRIH MERAH (Piper crocatum Ruiz and Pav.)

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Desi Maulida ◽  
Rugayah Rugayah ◽  
Tri Dewi Andalasari
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Block Design ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Randomized Block Design ◽  
The Difference ◽  
The Given

The research was conducted at the cutting red bettle (Piper crocatum Ruiz and Pav.) to determined the effect of (1) the difference growth of cuttings of red bettle which are given IBA and without IBA, (2) the concentration of NAA on growth of cutting red bettle, (3) the concentration of NAA on growth of cutting red bettle on each given IBA. The treatment was arranged in factorial (2 x 4) in randomized block design with three replication. The first factor were without IBA (A) and the given of 1000 ppm IBA (A1). The second factor were the concentration of NAA consists of: 0 ppm (B), 1000 ppm (B1), 2000 ppm (B2), and 4000 ppm (B). The results showed that the NAA concentration of 4000 ppm produced the most number of roots on either at the node or at the base of cutting. Planting cutting red bettle which given IBA 1000 ppm was able to accelerated the time leaves open and increased the number of cutting which germinate. The mixtured of giving IBA 1000 ppm and the concentration of NAA 4000 ppm, produced the most number of cutting that germinated.

scholarly journals Adventitious root formation in branch cuttings of Taxus wallichiana Zucc.(Himalayan yew): A clonal approach to conserve the scarce resource

2017 ◽  
Vol 8 ◽  
Author(s):  
Mohd Aslam ◽  
Parvaiz A. Raina ◽  
Rouf Ur Rafiq ◽  
T.O. Siddiqi ◽  
Zafar A. Reshi
Keyword(s):  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Block Design ◽  
Natural Habitat ◽  
Adventitious Root Formation ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Himalayan yew (<em>Taxus wallichiana</em> Zucc.), is an economically valuable plant and critically endangered due to overexploitation for the isolation of Taxol,an exciting anticancer drug from its bark and leaves. Since the species is unisexual and due to its long seed dormancy period and rapid loss of viability coupled with low survival percentage, its natural regeneration from seeds is very poor. As the seed raised plants add little growth, propagation by stem cuttings was tried under natural conditions and a considerable success was achieved after making use of different auxins (Indole-3- Acetic Acid-IAA, Indole Butyric Acid-IBA and Naphthalene Acetic Acid-NAA) in different concentrations. A Randomized block design (RBD) was adopted for laying the experiment of the present study. Of the ten treatments studied, IBA at 500ppm performed best of all the treatments and registered higher callusing percentage, rooting percentage, number of roots and length of roots in the juvenile shoot cuttings of the species. The results achieved through the application ofplant growth regulators (PGRs)by way of adventitious root formation (ARF) could be useful for the management of this understory coniferous tree species, whether for conservation, habitat restoration or for the production of Taxol,a promising anti-cancer agent. The technique evolved will be the most handy, quickest, inexpensive and can be applied any where in its natural habitat for the restoration and restocking of this valuable plant, which is otherwise facing the peril of extinction throughout the range of its distribution including Indian Himalayas.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.)

2015 ◽  
Vol 60 (3) ◽  
pp. 263-275 ◽  
Author(s):  
Fardin Nasri ◽  
Arsalan Fadakar ◽  
Mahmood Saba ◽  
Bayzid Yousefi
Keyword(s):  
Butyric Acid ◽  
Control Treatment ◽  
Rosa Damascena ◽  
Indole Butyric Acid ◽  
Significant Difference ◽  
Maximum Root Length ◽  
Number Of Leaves ◽  
Maximum Root ◽  
Medicinal Properties ◽  
The Rose

Rosa damascena is very important for essential oil production, medicinal properties and it is also widely cultivated as a garden rose. The Rose species is mainly propagated by stem cutting. In the present study, the effect of different levels of 0, 500 and 1,000 mg l-1 (quick dip method for 20 s) of indole butyric acid (IBA) on the rooting of 12 wild genotypes (including: Kurdistan 1 to Kurdistan 12) of R. damascena was investigated. The results show that the rooting ability of R. damascena differs significantly between the twelve genotypes. The highest rooting (79.56%) and callus production (69.08%), number of roots (8.33), root fresh and dry weights (361.80 and 244.74 mg, respectively) were recorded in Kurdistan 5 genotype with 1,000 mg l-1 IBA. The maximum root length (5.84 cm) was observed in Kurdistan 5 genotype with 500 mg l-1 IBA that showed a significant difference compared to the control treatment (0.96 cm). The highest number of leaves per bud (7.33 at 500 mg l-1 IBA) and number of buds (5.00 at 1,000 mg l-1 IBA) were recorded in Kurdistan 1 genotype. The current study demonstrated that the different genotypes of R. damascena were in a difficult-toroot state, which suggests that cutting treatment with 1,000 mg l-1 IBA overcame the problem of the difficult-to-root state, and it can also enhance the rooting percentage in the studied genotypes.

scholarly journals Development of a robust method for propagation of Leptospermum scoparium J.R.Forst. & G.Forst. from semi-hardwood cuttings

2020 ◽  
Author(s):  
Tate Jason James Hancox ◽  
Rachel A Burton ◽  
Kate Louise Delaporte
Keyword(s):  
Clonal Propagation ◽  
Natural Populations ◽  
Naphthalene Acetic Acid ◽  
Maturity Stage ◽  
Root Induction ◽  
Indole Butyric Acid ◽  
Transplant Survival ◽  
Detailed Method ◽  
Significant Difference ◽  
Leptospermum Scoparium

Abstract BackgroundLeptospermum scoparium J.R.Forst. & G.Forst. is highly valued for the production of antimicrobial honey. Both researchers and the Australian honey industry are interested in the clonal propagation of these plants to be used for experiments and plantation establishment. However, little information is available on clonal propagation by cuttings. Here we sought to develop a detailed method for L. scoparium propagation by semi-hardwood cuttings by testing the influence of genotype and auxin (indole butyric acid (IBA) and IBA + naphthalene acetic acid (NAA)) concentration on plants originating from four natural populations in Victoria, Australia. ResultsThere was no significant difference in root induction, which ranged from 50.9 – 68.0%, in the four clonal source plants selected. The application of 3 g/L IBA increased root induction from 46.6% of the control cuttings to 77.6% of treated cuttings and stimulated longer roots. The application of 8 g/L IBA increased leaf fall, the presence of necrosis, from 22.5% on the controls to 82.8% on dipped cuttings, and cutting death.Although there was no significant difference in root induction, cuttings that were ‘watered-in’ after planting dropped significantly fewer leaves and had significantly lower rates of necrosis than cuttings watered in after 2-3 days. It was also found that the maturity stage of semi-hardwood cuttings did not significantly affect any of the variables measured. Transplant survival ranged from 78.1 – 89.0% for cuttings treated with auxin and 77.3 to 92.2% for the four clonal source plants. ConclusionThis study found that the clonal source plants selected did not affect the success of propagation. Overall for maximum success, semi-hardwood cuttings can be collected from the current or previous seasons growth, be treated with 3 g/L IBA gel, ‘watered in’ and placed into a propagation tent for approximately six weeks. Cuttings that form roots will transplant easily with high transplant survival.

scholarly journals Determining the Rooting Ability of Hardwood Cuttings of Blueberry (Vaccinium corymbosum L.) cv. ‘Bluecrop’ Stored in Refrigerating Conditions

2020 ◽  
Vol 8 (3) ◽  
pp. 81
Author(s):  
Sabri Braha ◽  
Petrit Rama ◽  
Agim Zajmi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Roots ◽  
Vaccinium Corymbosum ◽  
Mother Plant ◽  
Winter Period ◽  
Naphthalene Acetic Acid ◽  
Intact Control ◽  
Rooting Ability ◽  
Time Periods

This experiment has been carried out in order to determine the rooting ability of hardwood cuttings of the blueberry cultivar ‘Bluecrop’ collected during the deep dormant winter period in the last part of January. In order to induce the adventitious roots formation, the cuttings were stimulated using Indol Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) in various concentrations (1500, 3000, 4500 ppm), whilst one row in each box remained intact (control).  The collection of cuttings off the mother plant at the beginning of January enable the nutrients and other matter that help the process of rooting not to move from the base of cutting towards the top and consequently they remain at the base of cutting which results with quite a high rooting percentage (up to 72.5%) compared to the cuttings collected in February with rooting percentage of 67.5% and in March (52%). In both types of substrate, turf and turf-perlite 2:1 and in different time periods when cuttings have been collected, IBA proved to be better in inducing adventitious roots compared to NAA.

scholarly journals Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1511-1519
Author(s):  
Alejandra Rojas Vargas ◽  
A Castander-Olarieta ◽  
P Moncaleán ◽  
I.A Montalbán
Keyword(s):  
Climate Change ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Pinus Radiata ◽  
Basque Country ◽  
Sequoia Sempervirens ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Forest Species ◽  
Forestry Sector

Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population and demand for forestry products results in overexploitation of forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change to have available to afforestation. For this reason, propagation methods should be improved to be more efficient in terms of quality and productivity. The main species planted in the Basque Country is Pinus radiata; during the last three years, Pinus radiata plantations have suffered a fungus attack affecting mainly needles until the tree's death. This crisis is caused by the combined action of two fungi of the genus Dothistroma and Lecanosticta acicola, whose expansion seems to have been enhanced by weather-related factors, such as humid and hot summers. Although we have evidence of this disease's presence in our mountains since 1942, the disease has had a speedy expansion with an aggressive effect for reasons that are not scientifically known today. For the above, Basque Country forestry sector is looking for alternative species to be used in its plantations. Part of the forestry sector considers that Sequoia sempervirens could be a good choice for plantations. Besides, its high-quality wood and its tolerance to the attack of several pathogens and other diseases derived from climate change are characteristics that could confer some advantage over other forest species. The main goal of this study was to optimize the micropropagation of adult elite trees of Sequoia sempervirens. The effect of 6-benzylaminopurine, meta-Topolin and Kinetin, and 4 types of explant in the multiplication stage were analyzed to carry out this objective. Furthermore, the effect of two types of auxins: 1-naphthalene acetic acid, indole-3-butyric acid, and a mixture of both, were evaluated on the induction of roots and their subsequent effect on the acclimatization process. The best multiplication index was obtained when 4.4 µM 6-benzylaminopurine and apical explants longer than 1.5 cm of length were used. The root induction percentage was 75% in the most responsive genotype analyzed when 4.4 µM 6-benzylaminopurine was used on the induction stage, and 50 µM of 1-naphthalene acetic acid was used for rooting. Finally, after 3 months in the greenhouse, the explants cultured with Kinetin and rooted in a culture medium with indole-3-butyric acid showed the highest acclimatization success (94%).

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