POTENSI KULTUR CAMPURAN BAKTERI ENDOFIT SEBAGAI PEMACU PERTUMBUHAN BIBIT TANAMAN KARET

Bakteri endofit adalah bakteri yang hidup dalam jaringan tanaman, dapat diisolasi melalui sterilisasi permukaan jaringan tanaman. Isolasi bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan sangat penting dilakukan. Pembuatan kultur campuran dari bakteri endofit diharapkan meningkatkan potensi dalam memacu pertumbuhan yang dapat meningkatkan kualitas bibit batang bawah tanaman karet. Penelitian ini dilakukan dengan tujuan mendapatkan kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Lima bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan yaitu Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, Brachybacterium paraconglomeratum LPD74, bacterium (bakteri tidak dikenal) LPD76, dan Providencia vermicola KPA38, diuji kompatibilitas untuk mendapatkan kultur campuran yang dapat meningkatkan pertumbuhan bibit batang bawah PB 260. Semua bakteri endofit terpilih kompatibel satu dengan yang lain. Aplikasi kultur campuran untuk meningkatkan pertumbuhan bibit batang bawah PB 260 memberikan hasil 2 kultur campuran terbaik. Kultur campuran 1 terdiri 2 spesies bakteri yaitu Brachybacterium paraconglomeratum LPD74 dan Providencia vermicola KPA38. Kultur campuran 2 terdiri 3 spesies bakteri yaitu Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, dan Brachybacterium paraconglomeratum LPD74. Bakteri endofit mampu masuk ke planlet bibit karet microcutting yang dibuktikan dengan Scanning Electron Microscopy. Diterima : 19 Mei 2014; Direvisi : 30 Mei 2014; Disetujui : 21 Juni 2014 How to Cite : Hidayati, U., Chaniago, I. A., Munif, A., Siswanto., & Santosa, D. A. (2014). Potensi kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Jurnal Penelitian Karet, 32(2), 129-138. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/159

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Scanning Electron Microscopy Study of Pseudomonas Aeruginosa in Vivo Adherence to Rat Bladder Epithelium

The Journal of Urology ◽

10.1016/s0022-5347(17)52949-3 ◽

1982 ◽

Vol 128 (2) ◽

pp. 415-417 ◽

Cited By ~ 3

Author(s):

J.D. Sobel ◽

Y. Vardi

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Bladder Epithelium ◽

Scanning Electron Microscopy Study ◽

Rat Bladder ◽

Scanning Electron

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Morphologic Expression of the Interactions of Human Lymphocytes and Pseudomonas aeruginosa as Observed by Scanning Electron Microscopy

The Journal of Infectious Diseases ◽

10.1093/infdis/139.1.18 ◽

1979 ◽

Vol 139 (1) ◽

pp. 18-25 ◽

Cited By ~ 2

Author(s):

B. A. Waisbren ◽

D. J. Hurley ◽

K. A. Siegesmund ◽

R. M. Guttman

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Human Lymphocytes ◽

Scanning Electron

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Antibacterial ativity of biosynthesized silver nanoparticles against Pseudomonas aeruginosa in vitro

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v41i1.81 ◽

2017 ◽

Vol 41 (1) ◽

pp. 60-65

Author(s):

Ahmad N. A. Salih ◽

Mohammad J. Eesa

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Silver Sulfadiazine ◽

Diffusion Method ◽

Olive Leaves ◽

Scanning Electron

This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-friendly and easy process and UV-Visible absorption spectra of the silver nanoparticles revealed maximum absorbance at 420 and 430 nm. The Scanning Electron Microscopy analysis demonstrated the mean of the silver particles diameter was 26 nm. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against Pseudomonas aeruginosa in vitro.

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Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v82i2.20 ◽

2016 ◽

Vol 82 (2) ◽

Author(s):

Irma KRESNAWATY ◽

Agustin Sri MULYATNI ◽

Deden Dewantara ERIS ◽

Haryo Tejo PRAKOSO

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Palm Oil ◽

Ft Ir ◽

Pha Production ◽

Palm Oil Mill ◽

Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh tiga isolat potensial, yaitu : satu isolat Pseudo-monas aeruginosa dan dua isolat Bacillus subtilis. Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa berbeda dengan Bacillus subtilisyang ditandai perbedaan pada spectra gugus C-C dan C-H alifatik. Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

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Optimization of the Antimicrobial Effects of Surfactin against Bacillus cereus Spores

Journal of Food Protection ◽

10.4315/jfp-20-032 ◽

2020 ◽

Vol 83 (11) ◽

pp. 1983-1988

Author(s):

XIANQING HUANG ◽

LIANJUN SONG ◽

MINGWU QIAO ◽

PINGAN ZHANG ◽

QIUYAN ZHAO

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Bacillus Cereus ◽

Control Sample ◽

Outer Wall ◽

Experimental Results ◽

Optimal Conditions ◽

Design Expert ◽

Temperature Time ◽

Scanning Electron

ABSTRACT The purpose of this study was to establish a three-variable bactericidal model of temperature, time, and concentration to determine the optimal conditions for Bacillus cereus spore inactivation by surfactin. To obtain the binary regression equation of the inactivated spore model, a total of 17 simulations were performed using response surface methodology. The experimental results showed that the three factors each had a discernible but nonequal impact on the inactivation response value. Multiple regression analysis of experimental results using Design-Expert software yielded the following equation: Y = 1.47 + 0.39ξ1(temperature) + 0.38ξ2(time) + 0.39ξ3(concentration) − 0.20ξ1ξ2 + 0.22ξ1ξ2 − 0.12ξ2ξ3 − 0.23ξ12 − 0.11ξ22 − 0.40ξ32. Optimal inactivation of spores was achieved by treatment with surfactin at a concentration of 4 mg/mL for 40 h at 53°C, with the response value reaching 1.8. The spores were treated with surfactin under these conditions; the microstructural changes of spores were observed by use of scanning electron microscopy. We found that the structures of the outer wall of the spores were damaged, whereas the spores in the control sample showed no visible damage. HIGHLIGHTS

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Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v82i2.20 ◽

2016 ◽

Vol 82 (2) ◽

Author(s):

Irma KRESNAWATY ◽

Agustin Sri MULYATNI ◽

Deden Dewantara ERIS ◽

Haryo Tejo PRAKOSO

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Palm Oil ◽

Ft Ir ◽

Pha Production ◽

Palm Oil Mill ◽

Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh tiga isolat potensial, yaitu : satu isolat Pseudo-monas aeruginosa dan dua isolat Bacillus subtilis. Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa berbeda dengan Bacillus subtilisyang ditandai perbedaan pada spectra gugus C-C dan C-H alifatik. Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

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Candida albicans enhances meropenem tolerance of Pseudomonas aeruginosa in a dual-species biofilm

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz514 ◽

2019 ◽

Vol 75 (4) ◽

pp. 925-935 ◽

Cited By ~ 2

Author(s):

Farhana Alam ◽

Dominic Catlow ◽

Alessandro Di Maio ◽

Jessica M A Blair ◽

Rebecca A Hall

Keyword(s):

Electron Microscopy ◽

Cystic Fibrosis ◽

Scanning Electron Microscopy ◽

Extracellular Matrix ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Medical Devices ◽

Fungal Infections ◽

Biofilm Structure ◽

Scanning Electron

Abstract Background Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices. Patients are prone to secondary fungal infections, with Candida albicans being commonly co-isolated with P. aeruginosa. Both P. aeruginosa and C. albicans are able to form extensive biofilms on the surfaces of mucosa and medical devices. Objectives To determine whether the presence of C. albicans enhances antibiotic tolerance of P. aeruginosa in a dual-species biofilm. Methods Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics. Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure. Results C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L. This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem. Conclusions We propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C. albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.

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Reducing Cr6+ in electroplating wastewater with Bacillus cereus strain B1

Universitas Scientiarum ◽

10.11144/javeriana.sc24-1.rcie ◽

2019 ◽

Vol 24 (1) ◽

pp. 73-89

Author(s):

Jennifer Andrea Moreno-Benavides ◽

Enrique Javier Peña-Salamanca ◽

Neyla Benítez-Campo

Keyword(s):

Waste Water ◽

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Cell Wall ◽

Bacillus Cereus ◽

Bacterial Growth ◽

Bacterial Cell ◽

Reduction Percentage ◽

Reducing Capacity ◽

Scanning Electron

In this study Bacillus cereus was used to evaluate the Cr6+ reducing capacity in electroplating effluents. TheCr6+ bioreduction assays were carried out using real waste water (RWW) from an electroplating plant and artificial wastewater (AWW), with and without added glucose. The AWW was prepared using Ni ,Zn ,Cu, Pb, and Cr salts, simulating RWW concentrations. The Cr6+ concentration and bacterial growth were monitored three times a day for the duration of five days. Also, were evaluated the effects of wastewater on B. cereus B1 morphology using scanning electron microscopy. Cr6+ reduction percentage of 100 % was reached for AWW containing glucose, of 71 % for AWW without glucose, of 75.6 % for RWW with glucose, and of 31.7 % for RWW without glucose. Despite the low reduction percentage obtained for RWW without glucose, concentrations of 0.14 mg/L of Cr6+ were reached, which were within allowed limits (0.5mg/L of Cr6+). Electron Microscopy showed alterations in the bacterial cell wall and a decrease in size, mainly in the bacteria exposed to RWW. The results allow us to propose B. cereus B1 as a promising microorganism for use in the bioremediation of effluents containing Cr6+.

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